Soil stabilization is a very important method of science and engineering for improving the properties of soil. This paper aims to stabilize expansive black cotton soil through a biological approach involving plant extracts, plant waste materials, and microorganisms. While chemical methods exist, i.e., lime stabilization, geotextiles, etc., they are not economically feasible for large-scale applications. The primary issue with black cotton soil is due to the presence of montmorillonite clay mineral, which makes it unsuitable for the construction of roads and airfields. The cation exchange capacity (CEC) can be defined as the ability of soil to absorb and exchange positively charged ions; thus, if free positively charged ions are not available, the soil will not exchange them with others. The CEC of the soil is diminished, and ultimately, the soil is stabilized to some extent. This paper explores the preparation of plant extract, which contains a high number of anions, and directly inoculates it with soil, which nullifies the positive charge of the soil and diminishes the CEC. The use of cellulose and lignin-degrading microorganisms as an energy source and other minerals that are needed for their growth will be utilized from the soil to reduce CEC, i.e., Mg required for DNA replication and Ca required for their growth and maintenance. Another approach to diminishing the CEC is to use the microorganisms that produce EPS, which require Ca and Mg as adhesions for the formation of biofilm, i.e., Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli. The use of microorganisms that have specific enzymes is also used in the diminishing soil CEC, i.e., by using ureolytic enzyme-producing bacteria like Sporosarcina pasteurii, Bacillus paramycoides, Citrobacter sedlakii, and Enterobacter bugadensis.
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