Cotinus Coggygria Scop Research Articles

The effect of IBA on anatomical changes and antioxidant enzyme activity during the in vitro rooting of smoke tree (Cotinus coggygria Scop.)

Induction of adventitious roots is crucial for the in vitro micropropagation of woody species. In this study the histological and biochemical aspects of the adventitious root formation in microcuttings of smoke tree were evaluated. Smoke tree shoots grown on Murashige and Skoog's (1962) medium with 1mgdm−3 N6-benzyladenine (BA) and 0.1mgdm−3 1-naphtaleneacetic acid (NAA) were transferred onto a rooting medium with or without 20mgdm−3 indole-3-butyric acid (IBA) and sampled at various intervals for histological and biochemical analyses of the rooting process. First cell divisions were observed on the first day of culture in the area of primary bark identifiable root primordia with an oval shape and several cell layers were present at Day 3 in cuttings on both media. Roots with organized tissue were observed 9days after the root induction on the medium with IBA; In shoots rooted on medium with IBA, the induction phase corresponded to a transient increase in the oxidase indole-3-acetic acid (IAAO) activity and levels of endogenous indole-3-acetic acid (IAA) and hydrogen peroxide (H2O2) coincidental with a reduction in the peroxidase (POX) and polyphenol oxidase (PPO) activity and the polyphenoloacids contents. An increase the PPO and POX activity, accompanied by a low activity of IAAO during phases of root development and elongation resulted in a reduction of the endogenous auxin and H2O2 contents in the IBA-treated cuttings.

Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats

Context: Cotinus coggygria Scop. (Anacardiaceae) leaves that were used as wound healing in traditional Balkan and Anatolian folk medicine, could be potentially effective in treating diabetic wounds.Objective: This study investigates biochemical and histological effects of ethanol extract of C. coggygria (CCE) on excision wound model in diabetic rats.Materials and methods: This study was conducted on diabetic Wistar albino rats, which were injected by a single dose (50 mg/kg i.p.) streptozotocin. Afterward an excision wound model was created in all animals; diabetic control rats were applied topically simple ointment and diabetic treatment rats were applied topically 5% (w/w) ointment with CC, once a day during the experimental period. Malondialdehyde, glutathione and hydroxyproline levels in wound tissues were investigated at the end of 3rd, 7th, and 14th days. Histopathological examination was also performed.Results: Hydroxyproline content was significantly increased in the CCE treated group versus control after the 3rd and 7th days (15.33 versus 11.83; 19.67 versus 15.67 mg/g, p < 0.05; respectively). A statistically significant elevation in glutathione at the end of 3rd, 7th, and 14th days (5.13 versus 1.58, p < 0.05; 4.72 versus 1.88, p < 0.05; 3.83 versus 1.88 μmol/g, p < 0.05, respectively) and a statistically significant decrease in malondialdehyde level at the end of 7th day (4.49 versus 1.48 nmol/g, p < 0.05) were determined in the treated group versus control group. These results were also supported by histological analyses.Discussion and conclusion: These findings indicate that CCE accelerated the cutaneous wound healing process in diabetic wounds, in confirmation of its traditional use.

Polyphenolic Profile and Targeted Bioactivity of Methanolic Extracts from Mediterranean Ethnomedicinal Plants on Human Cancer Cell Lines.

The methanol extracts of the aerial part of four ethnomedicinal plants of Mediterranean region, two non-seed vascular plants, Equisetum hyemale L. and Phyllitis scolopendrium (L.) Newman, and two Spermatophyta, Juniperus communis L. (J. communis) and Cotinus coggygria Scop. (C. coggygria), were screened against four human cells lines (A549, MCF7, TK6 and U937). Only the extracts of J. communis and C. coggygria showed marked cytotoxic effects, affecting both cell morphology and growth. A dose-dependent effect of these two extracts was also observed on the cell cycle distribution. Incubation of all the cell lines in a medium containing J. communis extract determined a remarkable accumulation of cells in the G2/M phase, whereas the C. coggygria extract induced a significant increase in the percentage of G1 cells. The novelty of our findings stands on the observation that the two extracts, consistently, elicited coherent effects on the cell cycle in four cell lines, independently from their phenotype, as two of them have epithelial origin and grow adherent and two are lymphoblastoid and grow in suspension. Even the expression profiles of several proteins regulating cell cycle progression and cell death were affected by both extracts. LC-MS investigation of methanol extract of C. coggygria led to the identification of twelve flavonoids (compounds 1–11, 19) and eight polyphenols derivatives (12–18, 20), while in J. communis extract, eight flavonoids (21–28), a α-ionone glycoside (29) and a lignin (30) were found. Although many of these compounds have interesting individual biological activities, their natural blends seem to exert specific effects on the proliferation of cell lines either growing adherent or in suspension, suggesting potential use in fighting cancer.

The Activity of Cotinus coggygria Scop. Leaves on Staphylococcus aureus Strains in Planktonic and Biofilm Growth Forms.

The purpose of this study was to detect the effectiveness of Cotinus coggygria Scop. leaves methanol extract against planktonic and biofilm growth forms of Staphylococcus aureus. The antimicrobial activity was determined by the broth microdilution test. Minimal inhibitory concentrations and minimal bactericidal concentrations were detected against two collection and ten clinical S. aureus strains. Anti-biofilm activity of the tested extract was detected using 24 h bacterial biofilm on the surface of microtiter plate wells. The biofilm inhibitory activity was evaluated visually after 24 h interaction of extract with biofilm, and the eradicating activity by a regrowth method. The tested extract showed bactericidal activity against all S. aureus strains (methicillin susceptible or methicillin resistant) in concentrations ranging from 0.313 to 0.625 mg·mL−1. Biofilm inhibitory concentrations were 10-times higher and biofilm eradicating concentrations 100-times higher (8 and 32 mg·mL−1, respectively). The phytochemical analysis of C. coggygria leaves 60% methanol extract performed by LC-DAD-MS/MS revealed quercetin rhamnoside, methyl gallate, and methyl trigallate as main constituents. Results of our study indicate that C. coggygria, rich in tannins and flavonoids, seems to be a prospective topical antibacterial agent with anti-biofilm activity.

Changes in the anti-inflammatory activity of aurone and chalcone class flavonoids from Cotinus coggygria extracts after complexation with cyclodextrins

Aurones and chalcones are less studied flavonoid classes with emerging therapeutical potential. Using the heartwood of European smoke tree (Cotinus coggygria Scop. syn Rhus cotinus L.) which contains aurones (sulfuretin, S) and chalcones (butein, B) [1], the aim of the present research was to obtain cyclodextrin (CDX) derivatives with enhanced water solubility and improved biological activity. Following steps were performed: 1) A flavonoid-enriched extract was prepared and standardized to its content in S, B, fustin, 2,3-dihydroquercetagetin, and quercetin using an established HPLC method [1]; 2) C. coggygria extract, S and B were each complexed through kneading with hydroxypropyl-beta cyclodextrin (HPBCD) and randomely methylated beta-cyclodextrin (RAMEB). A molar ratio of CDX: flavonoid = 2:1 was employed; 3) Formation of CDX complexes was confirmed by DSC, FT-IR and Karl Fisher titration techniques; 4) Anti-inflammatory effects of C. coggygria extract, S, B, and their CDX complexes were assessed in the model of the mouse ear edema (SKH1 male mice). Corneometry was used to measure the hydration level of the stratum corneum, this level being inversely correlated with skin irritation. Results showed that the noncomplexed extract, B, and S reduced inflammation with 50%, 33% and 50%, respectively. They are as well capable to lessen (with 26%, 97% and 30%, respectively) the skin dehydration induced by TPA. Complexation with CDXs enhances the anti-inflammatory effect of B (maximal for HPBCD) and S (maximal for RAMEB), but reduces it in case of the extract. These findings encourage further development of S- and B-CDX derivatives with improved biological effects.

Botanical Provenance of Historical Chinese Dye Plants

Botanical Provenance of Historical Chinese Dye Plants. Dye plants are an essential part of historical dyeing activities for textiles. Their correct botanical provenance is an important basis for understanding the sources mentioned in dyeing instructions and for chemical identification of the dyes on the textiles themselves. This paper describes a combined study of dyeing recipes in four historical manuscripts of the Ming (1368–1644 C.E.) and Qing Dynasties (1644–1911 C.E.). Within the context of historical plant medicine and botanical classification, this study resolves uncertainties about the names, provenance, and preferences for the significant dyes in Chinese textile history for the first time, namely safflower, sappanwood, smoketree, pagoda bud, Amur cork tree, indigo, Chinese gallnut, acorn cup, dark plum, munjeet, gromwell, gardenia, and turmeric. Most dye plants in the historical manuscripts refer to one or two major species or variants, with ambiguity arising mainly from non-uniform naming conventions and conversion between different taxonomies. It is now clear that saffron was confused with safflower in Ming Dynasty herbal plants instructions, that Su mu 蘇木 meant sappanwood (Caesalpinia sappan L.), and occasionally foreign redwoods from genera Caesalpinia and Haematoxylum, and that huang lu mu 黃櫨木, huang lu黃蘆 and lu mu 蘆/櫨木 were all variants of Cotinus coggygria Scop. Pagoda bud can be identified by huai hua 槐花, huai mi 槐米, huai hua mi 槐花米, and huai zi 槐子, while jiang huang 薑黃 and yv jin 郁金, recorded for dyeing, both referred to the rhizome of Curcuma longa L. Of the various zhi zi 梔子 available, zhi zi for dyeing was Gardenia jasminoindes Ellis f. longicarpa Z·W·Xie et M. Okada, although whether this preference was widely recognized or practiced is not clear. This research promotes a better understanding of historical Chinese dye plants. It also complements existing research results of historical herbal medicine in the aspect of dyeing.

English

English

Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential.

The Anacardiaceae Lindl. family comprises of many species which are used in nutrition and in traditional folk medicine for the treatment of several human diseases. Cotinus coggygria Scop. commonly known as “smoke tree”, is a commercial ornamental plant with high medicinal usages, belongs to the family Anacardiaceae. The present review provides a comprehensive report of empirical investigations on important pharmacological activities and phytochemical screening of essential oils and extracts. Relevant information was collected from scientific journals, books, and reports via library and electronic search using Medline, PubMed, Google Scholar, ScienceDirect, Web of Science, and Scopus. The plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. Numerous activities namely antioxidative, antibacterial, antifungal, antiviral, anticancer, antigenotoxic, hepatoprotective and anti-inflammatory have been demonstrated for all parts of these plants by in vivo and in vitro studies. Essential oils and extracts showed various pharmacological and biological properties which make them an effective remedy for various kinds of illnesses. Considering data from the literature, it could be demonstrated that C. coggygria possesses diverse bioactive properties and immense utilization in medicine, health care, cosmetics and as health supplements.

Progress of phytochemicals, pharmacological effects and clinical applications on Cotinuscoggygria Scop

Cotinus coggygria Scop. is the Anacardiaceae Cotinus L. plant and used for natural drugs in the treatment of acute icteric infectious hepatitis. The pharmacological research showed that Cotinus coggygria Scop. has the effects of reducing jaundice and enzyme, gallbladder Cholagogic and strengthen immune function. The current research on Cotinus coggygria Scop. is still not deep enough, the pharmacological effects of Cotinuscoggygria Scop. are mostly limited to the anticoagulant, hemolytic and anti liver chemical injury. In order to better study the scientific connotation of antitumor of Cotinus coggygria Scop., the active ingredient of Cotinuscoggygria Scop., pharmacological action and clinical application were reviewed. Key words: Cotinus coggygria Scop.; Phytochemicals; Pharmacological action; Clinical application

The effect of topical ethanol extract of Cotinus coggygria Scop. on cutaneous wound healing in rats

The aim of this study is to determine the cutaneous wound healing effects of the ethanol extract of Cotinus coggygria leaves in rats by excision wound model to provide scientific evidence for the traditional use of C. coggygria Scop. The levels of malondialdehyde, catalase, superoxide dismutase, glutathione and hydroxyproline were investigated in wound tissues. Histopathological examination was also performed. The hydroxyproline content of the granulation tissue and the glutathione levels were both significantly higher in the treatment group than in the control group (p < 0.05 for both); while the malondialdehyde levels were significantly lower in the treatment group (p < 0.05). These results were supported with histological results. The ethanol extract of C. coggygria Scop could be considered as an effective agent in wound healing in accordance with its traditional use.

Adaptive genetic variation in the smoke tree (Cotinus coggygria Scop.) is driven by precipitation

The landscape genetics of a widespread and locally adaptated species increases the understanding of the adaptive evolutionary process of local flora and vegetation. In this study, we selected Cotinus coggygria, which is widespread and locally adaptated species in China's warm-temperate zone, to investigate its landscape genetic pattern. We used eight microsatellite loci to examine the adaptive genetic variations of C. coggygria. A total of 43 microsatellite alleles were genotyped in 142 individual plants from 16 wild populations. The data demonstrated significant population differentiation within C. coggygria, which was caused by geographical distance, human activities, and precipitation. Five ecologically relevant microsatellite alleles, which were all related to the precipitation, were identified by association analysis. Our results indicate that precipitation is an important factor that drives adaptive genetic differentiation in C. coggygria.

Leaf structural reddening in smoke tree and its significance

The smoke tree (Cotinus coggygria Scop.) is not only an important ornamental tree that changes the color of its leaves in autumn but also maintains red juvenile leaves on vigorous sprouting shoots. To elucidate the process of leaf reddening in the smoke tree, we examined the structure and function of its leaf venation system, anthocyanin levels, leaf water conservation ability in red juvenile leaves and green mature leaves, leaf temperature, stomata conductance and snap strength of petiole. A combination of thermography, image analyses and photosynthetic measuring technology was used to study the facts of leaf reddening of smoke tree. We found that spatial and temporal heterogeneity in the pattern of leaf reddening in the smoke tree illustrates the interaction between internal metabolism and environmental factors. These results indicate that there are periods that show water and energy imbalances at leaf lamina and that this phenomenon is essential to leaf reddening, especially during periods of leaf expanding and leaf shedding. We consistently observed a relationship between high temperatures and leaf reddening, which indicates the latter is a photoprotective response induced by imbalances in water and energy.

Isolation and Characterization of Microsatellite Markers for Cotinus coggygria Scop. (Anacardiaceae) by 454 Pyrosequencing

Cotinus coggygria Scop. (Anacardiaceae) is a deciduous shrub or small tree that is native to a large area covering from southern Europe, east across central Asia, and the Himalayas in northern China. Shotgun 454 pyrosequencing was used to develop microsatellite markers from the genome of C. coggygra. In this study, 349 microsatellite loci were identified from 40,074 individual sequence reads produced by one-sixteenth run, and primer pairs were designed for these loci. To test the primer amplification efficiency, 50 microsatellite primer pairs were tested across 12 individuals from two C. coggygria populations (Wuzhi Mountain: 36°30'N, 113°39'E; Tianlong Mountain: 37°42'N, 112°26'E). Among the 50 tested primer pairs, eight were found to be polymorphic. The average allele number of the microsatellites was 3.5 per locus, with a range from two to five. The inbreeding coefficient ranged from −0.478 to 0.222. The observed and expected heterozygosities varied from 0.167 to 0.750 and from 0.163 to 0.743, respectively. This set of markers is potentially useful for assessing the genetic diversity, as well as for understanding the population structure and phylogeographical and landscape genetic patterns, of C. coggygria.

Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury

The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12h prior to administration of 100mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity.

Evaluation of the Antiviral Activity of Extracts from Plants Grown in the Qinling Region of China Against Infection by Tobacco mosaic virus (TMV)

AbstractTo investigate their antiviral potential, extracts from 126 plants grown in the Qinling region of China belonging to 103 plant species of 36 families were evaluated for antiviral activity against Tobacco mosaic virus. The activity of each plant extract on infection and replication was determined by local lesion and leaf‐disc methods. Extracts from nine species [Lactuca tatarica (Linn.) C. A. Mey, Rubus flosculosus Focke, Thermopsis lanceolata R. Br, Cotinus coggygria Scop Pulsatilla chinensis (Bunge) Regel, Thlaspi rvense Linn, Rodgersia podophylla A. Gray, Achnatherum splendens (Trin.) Nevski and Rhodiola eurycarpa (Frod.) S. H. Fu] strongly inhibited both infection and viral replication.

Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test

The genotoxic and antigenotoxic effects of Cotinus coggygria Scop. methanol extract was investigated using the Drosophila sex-linked recessive lethal (or SLRL) test. The results presented here show that the methanol extract of Cotinus coggygria in a concentration of 5% and artificial chemical agent ethyl methanesulfonate EMS (0.75 ppm) induce recessive lethal mutations on X-chromosome on Drosophila melanogaster in all broods (I, II and III). Post-treatment with lower concentration of the methanol extract of Cotinus coggygria (2%) was effective in reducing genotoxicity ofmutagen.

Identification of materials in post-Byzantine textiles from Mount Athos

Numerous textiles of inestimable historical significance have been preserved in the monasteries of Mount Athos for several centuries. However, our knowledge regarding the materials used in these objects is very limited. In the present study, microsamples extracted from ecclesiastical textiles (16th – 19th c.) of the monastery of Xeropotamou (Mount Athos) are investigated using Optical Microscopy (OM), Fourier Transform Infrared Spectroscopy (FTIR), High Performance Liquid Chromatography coupled to a Photodiode Array Detector (HPLC-PDA) and Scanning Electron Microscopy equipped with an Energy Dispersive X-ray spectrometer (SEM-EDX). The first goal of the study is to identify the colouring materials of the historical samples, which were of silk nature (OM). The presence of Prussian blue is revealed using FTIR and organic colourants such as cochineal, dyer’s broom ( Genista tinctoria L.), fuchsin, indigo carmine, old fustic ( Chlorophora tinctoria L.), soluble redwood ( Caesalpinia trees), weld ( Reseda luteola L.), young fustic ( Cotinus coggygria Scop.) and indigoid dyestuff source(s) are identified using HPLC-PDA. Dyestuff extraction from silk substrates is achieved using the TFA method, which preserves the contained glycosides. Thus, the report provides further evidence to support the efficacy of the TFA method to extract various organic colourants from historical silk samples (second goal). Finally, metal threads included in some historical samples are studied using OM and SEM-EDX. The latter is employed to analyse the composition of the metal surfaces. Silver (Ag) and gold (Au) are detected on the surfaces of the majority of the samples. Two samples were manufactured only with Ag.

Extract of the plantCotinus coggygriaScop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats

To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500mg·(kg body weight)(-1)) either 2or 12h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α(2)-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.

HPLC–DAD–MS analysis of dyes identified in textiles from Mount Athos

Organic colorants contained in 30 textiles (16th to early 20th century) from the monastery of Simonos Petra (Mount Athos) have been investigated using high-performance liquid chromatography equipped with diode-array detection and mass spectrometry (HPLC-DAD-MS). The components of natural dyes identified in samples treated by the standard HCl dyestuff extraction method were: alizarin, apigenin, butein, carminic acid, chrysoeriol, dcII, dcIV, dcVII, ellagic acid, emodin, fisetin, flavokermesic acid, fustin, genistein, haematein derivative (Hae'), indigotin, indirubin, isoliquiritigenin, isorhamnetin, kaempferide, kaempferol, kermesic acid, luteolin, naringenin, purpurin, quercetin, rhamnazin, rhamnetin, sulfuretin, and type B and type C compounds (last two are markers for Caesalpinia trees). Early, semi-synthetic dyes, for example indigo carmine, fuchsin components, and rhodamine B were identified in objects dated late 19th to early 20th century. A dyestuff extraction method which involves use of TFA, instead of HCl, was applied to selected historical samples, showing that the mild method enables efficient extraction of weld (Reseda luteola L.) and dyer's broom (Genista tinctoria L.) glycosides. The marker compound (Hae') for logwood (Haematoxylum campechianum L.) identification after treatment with HCl was investigated by liquid chromatography coupled to mass spectrometry (LC-MS) in negative electrospray ionization (LC-MS-ESI(-)) mode. LC-MS in negative atmospheric pressure chemical ionization (LC-MS-APCI(-)) mode was used, probably for the first time, to investigate cochineal (Dactylopius coccus Costa) samples. Positive electrospray ionization (LC-MS-ESI(+)) mode was used for identification of fuchsin components. Detailed HPLC-DAD studies were performed on young fustic (Cotinus coggygria Scop.) and Persian berries (Rhamnus trees).

High-speed counter-current chromatography: an effective method for the isolation of flavonoids and profisetinidin from fustic (Cotinus coggygria Scop.)

While chromatographic methods such as column chromatography and semi-preparative HPLC are widely employed during purifications of plant extracts, high-speed counter-current chromatography (HSCCC) is only occasionally utilized. This method based on liquid-liquid partition is advantageous as it avoids the loss of analytes due to irreversible adsorption. The present research focuses on the analysis of the diethyl ether-soluble fraction of a crude extract from Cotinus coggygria (Anacardiaceae) wood. After preliminary separation through vacuum liquid chromatography over RP-18 material (solvent: acetonitrile/water gradient), two of the resulted fractions (A-3, CH3CN/H2O 10/90, v/v and A-8, CH3CN/H2O 36/64, v/v) were purified on Sephadex LH-20 (solvent: methanol), yielding enriched fractions; four thereof were selected for HSCCC separations. An optimized solvent system was developed, comprising a mixture of hexane, ethyl acetate, methanol and water (1/2.5/1/1; all v/v). For separations, a P.C. Inc. (Potomac, USA; HSCCC multilayer coil, series 690) instrument was used. The system was operated in the „tail to head“ mode, with the upper solvent layer as mobile phase. HSCCC using the above solvent system afforded the isolation of seven compounds: fustin (1), dihydroquercetagetin (2), epifisetinidol-(4β→8)-(+)-catechin (3), fisetin (4), quercetin (5), butein (6), and the new natural compound C-3/C-3“ dimer of 3′,4′,7-trihydroxyflavanone (7). Their structure was elucidated by 1D (1H NMR, 13C NMR) and 2D (HSQC, HMBC) NMR experiments. The here described HSCCC solvent system is suitable for the separation of medium-polarity phenolic compounds from C. coggygria wood.