Costa Rica Research Articles

A new species of planthopper in the genus Colpoptera (Hemiptera:Fulgoroidea: Nogodinidae) from the Caribbean coast of Costa Rica

A new species of Colpoptera, C. stonei sp. nov., (Colpopterinae: Colpopterini) is described from Costa Rica, during a survey of planthoppers associated with palms. Molecular data for the five-prime region of the cytochrome c oxidase subunit I (COI) gene, 18S rRNA gene, and 28S rRNA gene are provided for available members of Colpopterini to support placement in Colpoptera.

Synopsis of the Castniidae (Lepidoptera) of Costa Rica

Based on an exhaustive review of the bibliography, and consultation of entomological collections around the world, we present an illustrated catalog with 16 Castniidae taxa present in Costa Rica. Corybantes veraguana veraguana (Westwood, 1877) is recorded for the first time in the country and new records are reported for rare and little-known species such as Athis analibiae (Espinoza-Sanabria & González, 2005), Athis delecta (Schaus, 1911) and Mirocastnia pyrrhopygoides smalli Miller, 1980. A taxonomic catalog of each taxon is included, as well as general information on geographic distribution, biogeography, ecology, seasonality, flight habits, material examined, and illustrations of males and females for all those taxa known from more than one specimen from Costa Rica.

Occurrence of pharmaceuticals in Latin America: case study on hazard assessment and prioritization in Costa Rica.

Pharmaceuticals are considered as contaminants of emerging concern, and their occurrence in diverse environmental matrices has been described during the last 25years. Nonetheless, pharmaceutical occurrence has not been evenly described worldwide, and reports from some geographical areas such as most parts of Latin America are scarce. This work aims to address the situation of water pollution due to pharmaceuticals in Latin America by means of two main goals: i. First, reviewing the monitoring studies performed in Latin America on this topic (period 2009-2024), which were conducted in Brazil, Mexico, Colombia, Ecuador, Peru and Argentina, to highlight the most frequently detected compounds from each therapeutic group in the region. ii. Second, analyzing the case of Costa Rica through the hazard assessment and prioritization of pharmaceuticals based on the monitoring performed in this country (years 2011; 2018-2019). The monitoring in Costa Rica comprised a total of 163 sampling points: wastewater treatment plants (WWTPs) (14 urban WWTPs plus two landfill WWTPs; total samples n = 44 influents and n = 34 effluents), nine hospital effluents (n = 32), wastewater from livestock farms (six swine farms and seven dairy farms; n = 23 influents and n = 37 effluents), 64 continental surface water sampling points (n = 137), and 61 coastal seawater sampling points (n = 61). Risk assessment of detected concentrations by the hazard quotient (HQ) approach (period 2018-2019) revealed a total of 25 mediumorhigh-hazard compounds (out of 37 detected compounds). The prioritization approach (which included the Frequency of Appearance (FoA), the Frequency of PNEC exceedance (FoE), and the Extent of predicted no-effect concentration (PNEC) exceedance (EoE)), showed a critical list of nine pharmaceuticals: caffeine, diphenhydramine, acetaminophen, lovastatin, gemfibrozil, ciprofloxacin, ibuprofen, doxycycline and norfloxacin. These compounds should be taken into account as a first concern during the implementation of environmental policies related to pharmaceutical products in the region.

American Cutaneous Leishmaniasis: Imported cases in Berlin 2000-2023.

American Cutaneous Leishmaniasis (ACL) shows variable response to therapy, but data on species-specific treatment efficacy is scarce. We describe the clinical characteristics and outcome of patients with ACL imported to a tertiary centre in Germany and determine whether species-specific therapy according to the 2014 "LeishMan" group recommendations is associated with cure. A retrospective chart review was conducted at the Charité Institute of International Health in Berlin. We analysed data on PCR-confirmed ACL cases collected between 2000 and 2023. Systemic therapy included liposomal amphotericin B, miltefosine, pentavalent antimony, ketoconazole or itraconazole. Localized therapy included perilesional pentavalent antimony or paromomycin ointment. Cure was defined as re-epithelialization of ulcers or disappearance of papular-nodular lesions after 3 months of treatment. Logistic regression models were used to quantify the effect of species-specific systemic therapy on the outcome. 75 cases were analysed. Most patients were male (62%), median age was 35 years, no patient had a history of immunosuppression. The most common reason for travel was tourism (60%), the most common destination was Costa Rica (28%), the median duration of illness was 8 weeks, and most patients presented with ulcers (87%). Lesions were complex in 43%. The most common Leishmania (L.) species was L. braziliensis (28%), followed by L. panamensis (21%). 51/73 (70%) patients were cured after initial therapy and 17/21 (81%) after secondary therapy. Cure after systemic therapy was more frequent when species-specific treatment recommendations were followed (33/45; 73%), compared to when not followed, (6/17; 35%, P = 0.008). This association was independent of age, sex, previous therapy, complex lesions, and Leishmania species (adjusted OR, 5.06; 95% CI, 1.22-24.16). ACL is a rare, imported disease in Germany. Complex lesions were common, challenging successful therapy. This study highlights the importance of identifying the parasite species and suggests that a species-specific approach to treatment leads to better outcomes.

Lipid nanoparticle mRNA therapy improves survival and reduces serum branched-chain amino acids in mouse models of maple syrup urine disease.

Maple syrup urine disease (MSUD) is a rare, inherited, metabolic disorder characterized by dysfunction of the multi-subunit, mitochondrial enzyme complex branched-chain alpha-keto acid dehydrogenase (BCKDH). BCKDH catalyzes the oxidative decarboxylation of branched-chain amino acids (BCAAs). BCAAs and their neurotoxic alpha-keto intermediates can accumulate in the blood and tissues in the absence of functional BCKDH. We evaluated a lipid nanoparticle (LNP)-based treatment approach to address all possible genetic mutations that can cause MSUD (BCKDHA, BCKDHB, and DBT). In the intermediate MSUD mouse model, which harbors a mutation in the dihydrolipoamide branched-chain transacylase E2 (DBT) subunit of BCKDH, repeated administration of LNP-encapsulated mRNA therapy significantly extended survival and reduced serum leucine levels. We also evaluated our LNP approach in several models of classic MSUD, namely DBT knockout (KO) mice and the new BCKDHA KO and BCKDHB KO mice. The latter two were generated by CRISPR/Cas9 gene editing and contain the highly prevalent classic MSUD-causing mutations seen in the Mennonite and Costa Rican populations. Intravenous LNP-encapsulated mRNA administration extended survival and increased body weight in the DBT KO and BCKDHA KO models of classic MSUD but was not effective in BCKDHB KO mice. Our data provide a promising proof-of-concept that a universal, mutation-independent approach to treating MSUD is possible and viable.

Spectrum of Oral Lesions Histopathologically Diagnosed at the Faculty of Dentistry, Universidad de Costa Rica

Spectrum of Oral Lesions Histopathologically Diagnosed at the Faculty of Dentistry, Universidad de Costa Rica

Changes in the economics of coffee production between 2008 and 2019: a tale of two Central American countries

Increasing costs of coffee production relative to coffee prices has led to concern across the industry of lack of profitability of coffee production especially for smallholders who comprise a large majority of producers. This study compares coffee production costs and income over a decadal interval of 2008 versus 2019 for coffee farmers in some of the main coffee growing regions of Costa Rica and Guatemala. Costs and income were collected by farmer recall using a standard questionnaire with trained research surveyors. Net income as assessed by EBITDA (earnings before interest, taxes, depreciation and amortization) increased by about 30% in Costa Rica, but declined to a third of its 2008 level in Guatemala. Agronomic costs of production per hectare increased by 31% in Costa Rica and 62% in Guatemala, mostly due to increased labor costs (higher daily wage rates), while fertilizer usage increased but unit costs remained stable. Gross income was stable in Guatemala but increased in Costa Rica due to receiving significantly higher prices for their coffee in 2019 compared to 2008, while in Guatemala prices declined. Nevertheless, the response was not uniform between farms in Costa Rica while high and medium productivity groupings of farms had higher EBITDA, low and very low productivity farms experienced a decline similar to Guatemala. The difference in performance of farm groups in Costa Rica was due to a decline in production per hectare of the lower productivity group; while the difference between Guatemala and Costa Rica was firstly due to price differences, and secondarily due to lower productivity of some farm groups. The investment of Costa Rican farmers was undoubtedly supported by the substantially increased price received by farmers (as compared to Guatemala), reflected in the increase in export price of coffee from Costa Rica relative to Guatemala. This shows the importance of farmers receiving higher prices for their produce in enabling them to cover increasing production costs, invest in increasing productivity and maintain profitability.

Bocconia frutescens little leaf, a new plant disease associated to a ´Candidatus Phytoplasma pruni´ related strain in Costa Rica

Objective/Background. Bocconia frutescens (Papaveraceae) is a small tree distributed naturally from Mexico to Argentina and the Caribbean Bassin. Bocconia trees showing symptoms resembling phytoplasmas infection, such as little leaves and witches´-broom, were found in Cartago province, Costa Rica. Detection and identification of the potential phytoplasmas associated with B. frutescens little leaf symptoms was the objective out of this study. Materials and Methods. Evaluation of leaves tissue using transmission electron microscopy (TEM), nested PCR using universal and specific primers to amplify phytoplasmas 16S rRNA and secA genes. Nucleotidic sequences (Sanger method) were obtained from amplicons, and used for BLASTn, phylogenetic analyses, and in silico RFLP’s. Results. Presence of phytoplasmas into phloem tissue, only in symptomatic trees, was evidenced by TEM. Comparison of partial sequences (16Sr and secA genes) by BLASTn, in silico RFLP´s and phylogenetic analyses, showed the occurrence of a ´Candidatus Phytoplasma pruni´ related strain in the samples evaluated. Conclusion. Phytoplasmas were found only in the symptomatic B. frutescens trees evaluated. The phytoplasmas were identified as a ´Ca. Phytoplasma pruni´ related strain. This is the first report of B. frutescens as a natural host of ´Ca. Phytoplasma pruni´.

When the predator becomes the prey: new records of intraguild predation among Central American and Caribbean arachnids (Arachnida: Amblypygi, Araneae, Scorpiones)

ABSTRACT Intraguild predation occurs when an organism preys on a competitor. This ecological interaction can have considerable consequences on the population dynamics of predators and indirectly affect their prey. In arachnids, it is a quite common phenomenon, but it has been poorly documented in the Neotropical region. Here, we present records of 16 species feeding on other 14 species of arachnids. The most common families that acted as predators were Buthidae (Scorpiones) and Salticidae (Araneae), while the most common prey were members of the families Araneidae and Ctenidae (Araneae). In our survey, Drake Bay, in Costa Rica, is overrepresented due to years of systematic observations by two of the coauthors.

When the predator becomes the prey: new records of intraguild predation among Central American and Caribbean arachnids (Arachnida: Amblypygi, Araneae, Scorpiones)

ABSTRACT Intraguild predation occurs when an organism preys on a competitor. This ecological interaction can have considerable consequences on the population dynamics of predators and indirectly affect their prey. In arachnids, it is a quite common phenomenon, but it has been poorly documented in the Neotropical region. Here, we present records of 16 species feeding on other 14 species of arachnids. The most common families that acted as predators were Buthidae (Scorpiones) and Salticidae (Araneae), while the most common prey were members of the families Araneidae and Ctenidae (Araneae). In our survey, Drake Bay, in Costa Rica, is overrepresented due to years of systematic observations by two of the coauthors.

Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean Countries.

The purpose of this article is to describe the activities developed within the framework "Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean countries," developed between October 16th and 19th of the year 2023 in the city of San José, Costa Rica. The workshop was carried out as part of a joint work between the Pan American Health Organization (PAHO) and the World Health Organization (WHO), in cooperation with the International Atomic Energy Agency (IAEA). The main objective of the regional workshop was to gather the experiences and future work planning among participants in the Optimization of Protection in Pediatric Interventional Radiology in Latin America and the Caribbean (OPRIPALC) program. It involved professionals from 14 centers across 11 countries in the region, along with 4 experts from PAHO/WHO/IAEA. The work modalities during the workshop consisted of keynote presentations, individual presentations, group work, and general discussions. An online survey was carried out after the workshop, with the objective of knowing the opinion of the event participants and determining the impact and projection of the OPRIPALC program. During the workshop the centers had to present their experiences: the use of the DOLQA dose management system was presented and work was done on the consensus document on good practices. The activities, topics and organization of the workshop were valued positively by the participants. There is unanimity among the centers that the OPRIPALC program has had a positive impact and they wish to continue actively participating in the next biennium.

Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean Countries.

The purpose of this article is to describe the activities developed within the framework "Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean countries," developed between October 16th and 19th of the year 2023 in the city of San José, Costa Rica. The workshop was carried out as part of a joint work between the Pan American Health Organization (PAHO) and the World Health Organization (WHO), in cooperation with the International Atomic Energy Agency (IAEA). The main objective of the regional workshop was to gather the experiences and future work planning among participants in the Optimization of Protection in Pediatric Interventional Radiology in Latin America and the Caribbean (OPRIPALC) program. It involved professionals from 14 centers across 11 countries in the region, along with 4 experts from PAHO/WHO/IAEA. The work modalities during the workshop consisted of keynote presentations, individual presentations, group work, and general discussions. An online survey was carried out after the workshop, with the objective of knowing the opinion of the event participants and determining the impact and projection of the OPRIPALC program. During the workshop the centers had to present their experiences: the use of the DOLQA dose management system was presented and work was done on the consensus document on good practices. The activities, topics and organization of the workshop were valued positively by the participants. There is unanimity among the centers that the OPRIPALC program has had a positive impact and they wish to continue actively participating in the next biennium.

Geomorphology, morphodynamic, and surficial material of the Orosi Valley, Costa Rica

The Orosi Valley in Costa Rica holds significant geomorphological importance due to its diverse landscapes shaped by tectonic activity, river systems, erosion processes, and rich biodiversity. Moreover, this valley is important for agriculture, tourism, water reservoirs, and hydroelectricity production. This study presents a comprehensive geomorphological mapping of the Orosi Valley in Costa Rica. Six distinct geomorphological units have been identified and classified through a combination of field surveys, topographic data analysis, and remote sensing techniques. These units include hillslopes, alluvial fans, terraces at the valley margins, volcanic landforms, colluvial deposits, landslide scars, and fluvial landforms. The results provide valuable insights into the geomorphological processes and landform evolution within the Orosi Valley, contributing to scientific knowledge, land use planning, natural hazard assessment, and environmental management in the region. This study holds global importance by deepening the understanding of how geomorphology, disaster risk, and socio-economic development are interconnected. It informs worldwide initiatives to create resilient communities in response to evolving environmental hazards.

Geomorphology, morphodynamic, and surficial material of the Orosi Valley, Costa Rica

The Orosi Valley in Costa Rica holds significant geomorphological importance due to its diverse landscapes shaped by tectonic activity, river systems, erosion processes, and rich biodiversity. Moreover, this valley is important for agriculture, tourism, water reservoirs, and hydroelectricity production. This study presents a comprehensive geomorphological mapping of the Orosi Valley in Costa Rica. Six distinct geomorphological units have been identified and classified through a combination of field surveys, topographic data analysis, and remote sensing techniques. These units include hillslopes, alluvial fans, terraces at the valley margins, volcanic landforms, colluvial deposits, landslide scars, and fluvial landforms. The results provide valuable insights into the geomorphological processes and landform evolution within the Orosi Valley, contributing to scientific knowledge, land use planning, natural hazard assessment, and environmental management in the region. This study holds global importance by deepening the understanding of how geomorphology, disaster risk, and socio-economic development are interconnected. It informs worldwide initiatives to create resilient communities in response to evolving environmental hazards.

Shaping the future: Proposed bills to regulate new and emerging tobacco and nicotine products in Latin America and the Caribbean amidst public health concerns and industry interests.

To examine legislative efforts to regulate new and emerging tobacco and nicotine products (NETNPs) in Latin America and the Caribbean (LAC) between 2017 and 2022. Analysis of on-line national legislation databases to track NETNP bills using standard snowball search methods. Bills were identified in 15 out of the 33 LAC countries, accounting for a total of 91 bills targeting NETNPs (Table 1). Mexico had the highest number (n=38) of bills, followed by Brazil (n=16), Argentina (n=10), Colombia (n=8) and Chile (n=5). The remaining countries introduced two or fewer NETNP bills. Among the identified 91 bills, the majority (n=60) addressed both electronic cigarettes and HTPs, almost one third of bills (n=29) applied only to electronic cigarettes, and only two focused solely on HTPs. Six of the 15 countries with identified bills, enacted one NETNP law including Barbados, Bolivia, Costa Rica, Guyana, Mexico, and Panama. Since 2019, there has been a steady increase in the amount of NETNP bills introduced in LAC. In 2017 and 2018, 10 NETNP bills were introduced each year followed by 23 NETNP bills introduced in each 2019 and 2020, 19 in 2021, and 16 by August 2022. The substantial increase in the number of NETNP bills introduced since 2019 indicates the growing importance of regulating NETNPs in LAC. As NETNPs continue to evolve and grow it is important to continue monitoring and evaluating tobacco and nicotine regulatory initiatives while also staying ahead of the evolving market of NETNPs. Previous studies have analyzed the regulatory environment of new and emerging tobacco and nicotine products (NETNPs) but it is less known about legislative and regulatory efforts and attempts. This is the first known study to analyze legislative bills to regulate NETNPs in Latin America and the Caribbean (LAC). The substantial increase in the number of NETNP bills introduced in LAC since 2019 indicates the growing importance of regulating NETNPs in LAC. As NETNPs continue to evolve and grow it is important to continue monitoring and evaluating tobacco and nicotine regulatory initiatives while also staying ahead of the evolving market of NETNPs.

Traditional rural dietary pattern and all-cause mortality in a prospective cohort study of elderly Costa Ricans: the Costa Rican Longevity and Healthy Aging Study (CRELES)

BackgroundCosta Rica is experiencing a fast demographic aging. Healthy diets may help to ameliorate the burden of aging-related conditions. ObjectiveTo investigate the association of a traditional dietary pattern and two of its major components (beans and rice) with all-cause mortality among elderly Costa Ricans. DesignThe Costa Rican Longevity and Healthy Aging Study (CRELES) a prospective cohort study of 2,827 elderly Costa Ricans (60+ years at baseline) started in 2004. We used a food frequency questionnaire (FFQ) to assess usual diet. We calculated dietary patterns using Principal Component Analysis (PCA). Multivariate energy-adjusted proportional-hazards models were used to estimate hazard ratios (HR) and 95% confidence intervals (CI). ResultsOver a 15-year follow-up, encompassing 24,304 person-years, 1,667 deaths occurred. The traditional Costa Rican dietary pattern was more frequent in rural parts of the country, and it was inversely associated with all-cause mortality. Subjects in the fifth quintile of intake had 18% lower all-cause mortality compared with those in the first quintile (HR = 0.82; 95% CI = 0.69, 0.98; P-trend = 0.01), particularly among males (HR = 0.73; 95% CI = 0.56, 0.95). Beans intake was associated with lower all-cause mortality among all subjects (HR = 0.79; 95% CI = 0.68, 0.91 highest compared with lowest tertile) and in sex-stratified analysis. Rice consumption was inversely associated with all-cause mortality solely among males (HR = 0.75; 95% CI = 0.60, 0.94 highest compared with lowest tertile). ConclusionsOur results suggest that a traditional Costa Rican rural dietary pattern is associated with lower all-cause mortality in elderly Costa Ricans. Beans, a major component of this traditional dietary pattern, was also associated with lower all-cause mortality. These findings could have important implications for public health, given the nutritional transition and the reduction of intake of traditional diets in Latin American countries.

Hematology and serum biochemistry of free-ranging mantled howler monkeys (Alouatta palliata) at La Pacifica, Costa Rica.

Hematologic and blood biochemical values are key tools for assessing primate health. A long-term behavioral study of howler monkeys at a single site (La Pacífica, Guanacaste, Costa Rica), afforded the opportunity to develop baseline values for a large group of animals, evaluating differences between adult males and females and comparing to a report in the same population two decades later. In 1998, 64 free-ranging mantled howler monkeys were anesthetized and sampled for hematologic and biochemical analysis. Blood analysis is reported for 29 adult females, 9 juvenile females, 19 adult males and 3 juvenile males. Four adults were excluded due to external injury or disease. There were few significant differences between adult females, juvenile females, and adult males. Baseline blood parameters are useful for determining normal values for howler monkey populations. The values for total protein, blood urea nitrogen, glucose, liver enzymes and potassium differed from a later study in 2019 may indicate changes that are influencing howler monkey health.

Social disparities in flood exposure and associations with the urban environment in 44,698 neighborhoods in 276 cities in eight Latin American countries.

Climate change is expected to greatly increase exposure to flooding, particularly in urban populations in low- and middle-income countries. We examined within-city social disparities in exposure to flooding in 276 Latin American cities and associated features of the neighborhood urban environment. We used a spatially granular dataset of historical flood events from 2000 to 2018 to describe neighborhood flooding within cities across eight Latin American countries (Argentina, Brazil, Chile, Colombia, Costa Rica, Guatemala, Mexico, and Panama). We estimated the percentage of the population living in flooded neighborhoods, described social disparities in flooding based on neighborhood educational attainment, and compared the magnitude of disparities across and within cities. We used multilevel models to examine how city- and neighborhood-level factors are related to neighborhood flooding. We examined 44,698 neighborhoods in 276 cities from eight countries with a total of 223 million residents and 117 distinct flood events from 2000-2018. One in four residents in neighborhoods in the lowest education quintile lived in neighborhoods with flooding, compared to one in 20 residents of the highest neighborhood education quintile. Greater neighborhood flooding was associated with lower neighborhood-level educational attainment and with neighborhoods that were coastal, less dense (population or intersection), further from the city center, greener, and had steeper slopes. There was no association between city-level educational attainment and flooding. There are large social disparities in neighborhood flooding within Latin American cities. Residents of areas with lower education attainment face substantially higher risks of flooding. Policymakers must prioritize flood adaptation and recovery efforts in neighborhoods with lower socioeconomic position.

Metáfora conceptual y embodiment en la noción «Costa Rica verde» en Esencial Costa Rica

A semantic analysis is presented of the advertising language used by the brand Essential Costa Rica. A measurable strategy based on the conceptual metaphor “green is gold” is evident in these texts. It is examined using two macrocategories present in the words and expressions that make up the brand’s tourism advertising: “Costa Rica awaits a treasure” and “Costa Rica is a paradise.” They both generate an identity image of the region, apparently idyllic and paradisiacal, where socio-environmental problems typical of the reality that the country faces are distorted and become invisible.

A Comparative Approach for Pronunciation Instruction in English

A tool is proposed to reinforce the pronunciation of vowels and consonants for the instruction of adult learners in Costa Rica. A critical review of literature on approaches to teaching vowels and consonants is followed by a comparative discussion of two transcription systems: that of the International Phonetic Association, and those found in textbooks, dictionaries and pronunciation websites. This is complemented by a brief comparison of English and Spanish vowels and consonants, and descriptions of common pronunciation challenges for Spanish speakers. Open problems are explored, as are ethical reflections on the teaching of English pronunciation.