Background: Cervical cancer is the 2nd commonest cancer among women worldwide and is the leading cause of cancer deaths among women in developing countries. Optimal cervical cancer screening uptake is one of the most cost effective control strategies for the disease. Objectives: To assess the determinants of cervical cancer screening uptake among women in Orlu Local Government Area, Imo State, Nigeria. Materials & Methods: A community based cross-sectional study with the use of 502 interviewer-administered questionnaires was conducted among women in Orlu Local Government Area of Imo State. All the women in the community attending the annual 2018 August meeting were recruited for this study. Results:The study showed that the mean age of the respondents was 42±2.8 years. Furthermore, 82.3% of respondents attained postsecondary school educational level. Also 80.5% of respondents have heard about cervical cancer screening but level of cervical cancer screening uptake was very low among the respondents (13.5%). Significant associations were found between embarrassment, lack of symptoms, lack of physician request, lack of counselling, cost, unavailability of services and cervical cancer screening uptake. Conclusion: Despite the high knowledge of cervical cancer screening among women in Orlu LGA, uptake of cervical cancer screening was low. Enhancing health education and provision of free and widely accessible health screening services may increase uptake among these women. Key words: Cervical cancer, screening, Orlu women, uptake.