Cosmetic Skin Research Articles

Skin Improvement Effects of Ultrasound-Enzyme-Treated Collagen Peptide Extracts from Flatfish (Paralichthys olivaceus) Skin in an In Vitro Model.

Collagen is considered to be an intercellular adhesive that prevents tissue stretching or damage. It is widely utilized in cosmetic skin solutions, drug delivery, vitreous substitutions, 3D cell cultures, and surgery. In this study, we report the development of a green technology for manufacturing collagen peptides from flatfish skin using ultrasound and enzymatic treatment and a subsequent assessment on skin functionality. First, flatfish skin was extracted using ultrasound in distilled water (DW) for 6 h at 80 °C. Molecular weight analysis via high-performance liquid chromatography (HPLC) after treatment with industrial enzymes (alcalase, papain, protamex, and flavourzyme) showed that the smallest molecular weight (3.56 kDa) was achieved by adding papain (0.5% for 2 h). To determine functionality based on peptide molecular weight, two fractions of 1100 Da and 468 Da were obtained through separation using Sephadex™ G-10. We evaluated the effects of these peptides on protection against oxidative stress in human keratinocytes (HaCaT) cells, inhibition of MMP-1 expression in human dermal fibroblast (HDF) cells, reduction in melanin content, and the inhibition of tyrosinase enzyme activity in murine melanoma (B16F10) cells. These results demonstrate that the isolated low-molecular-weight peptides exhibit superior skin anti-oxidant, anti-wrinkle, and whitening properties.

Natural active ingredients in cosmetics for mature skin

The human skin performs key functions in the human body, while also being a reflection of the many changes occurring within it. The face, which is most exposed to external factors, is the area where the skin ageing process progresses most rapidly. The study aimed to review the most commonly used active ingredients in natural cosmetics for mature skin, available on the Polish cosmetics market. Raw materials of natural origin are a rich source of active substances, which are widely used in cosmetics. These substances not only moisturise, nourish, cleanse, soften and smooth the skin, but also stimulate circulation in blood vessels, eliminating redness and swelling. The use of active ingredients of natural origin can effectively maintain mature skin in good condition, significantly delaying the ageing processes occurring within it.

FABRICATION AND PHYSIOCHEMICAL CHARACTERISATION OF MEDIUM MOLECULAR WEIGHT HYALURONIC ACID COPPER OXIDE BASED GBR MEMBRANE

Introduction: Hyaluronic acid, as a natural linear polysaccharide, has attracted researchers’ attention from its initial detection and isolation from tissues in 1934 until the present day. Hyaluronic acid has a wide range of uses in bioengineering and biomedicine because of its biocompatibility and high biodegradation rate, from polymeric scaffolds and wound dressings to endoprostheses for joint fluid and biorevitalizing skin cosmetics. The aim of the study was to find about fabrication and Physicochemical characterization of medium molecular weight hyaluronic acid /CuO GBR membrane.

Formulation and evaluation of herbal scrub soap

Scrubbing Soap are the agents which are use to remove the dead cell from skin, pigmentation and blackheads, white heads and make skin glowing, smooth, soft and healthy. Scrubbing soap can be directly applied onto the skin by gently massage is recommended on application of the scrubbing soap which helps to improve blood circulation and increase oxygen supply to all surface of the skin. Due to some environmental factors many people face some problem like pigmentation and uneven skin tone. Solution for this problem is use of scrubbing soap which consist ingredients which increases cleansing, softening, moisturizing, fairness of skin. In this preparation neem, tulsi and poppy seeds are use as main active ingredients. Other ingredients like glycerin base, essential oil and rose water, ritha powder, alovera, turmeric vitamin E are use in the formulation. Thus, the developed formulation can be used as an effective scrubbing soap for using it to bear a healthy and glowing skin. Ayurvedic cosmetics are also known as the herbal cosmetics the natural content in the herbs does not have any side effect on the human body most herbal supplement are based on several botanical ingredients with long histories of traditional or folk medicine usage. Among the numerous botonical ingredients available in the market today. Numerous chemical toxins microorganism present in the atmosphere may cause chemical infection and damage to skin cosmetics alone are not sufficient to take care of skin and body parts. Neem (Azadirachta indica) tree has attracted worldwide prominance owing to its wide range of medicinal properties, neem leaves and its constituents have been demonstrated to exhibit anti–inflammatory, antihyperglycemic, antiulcer, antimalarial, antifungal, antibacterial, antimutagenic and anticarcinogenic properties. This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extract from neem leaves.

Formulation and Evaluation of Herbal Soap

Numerous chemical toxins and microorganisms present in the The atmosphere may cause chemical infection and damage to the Skin cosmetics alone are not sufficient to take care of the Skin. Herbal soap ingredients use neem, tulsi, and turmeric and alovera were found effective against Dermatophytes, turmeric shows anti-inflammatory and Anti-bacterial properties and tulsi shows anti-viral Activity.

Experience of using drospirenone/ethinyl estradiol combination in women of reproductive age

Introduction. A woman’s unwillingness to enter pregnancy due to social and somatic factors is associated with a high risk of its termination and concomitant complications. A personalized selection of a method of contraception is necessary, which best meets the needs of a woman, including in achieving non-contraceptive effects.Aim. To evaluate the effectiveness, safety and compliance indicators when using a combined drug (3 mg of drospirenone + 20 mcg of ethinyl estradiol) in women in the early and mature reproductive period.Materials and methods. A study involving 68 women (polycystic ovary syndrome (PCOS), n = 31, adenomyosis, n = 20, uterine fibroids, n = 7, a combination of 2 or more diagnoses) was conducted on the basis of the Scientific polyclinic Department of the V.I. Kulakov National Research Medical Center of the Ministry of Health of the Russian Federation) of reproductive age (18–34 years) who took a combined contraceptive drug for 6 months or more. The control of the results was carried out on the basis of monitoring in the form of a survey.Results. The average time of use of the drug was 9 (Q₁–Q₃: 8–11) months. After 3 and 6 months, 77.8% (21/27) and 100% of the observed patients, respectively, noted a decrease in the severity of PMS symptoms. 91.7% (n = 11/12) of patients noted a subjective decrease in the severity of acne and/or hirsutism after 6 months. 91.3% (n = 21/23) of women with copious painful menstruation noted normalization of the bleeding profile (volume of menstrual blood loss). 95.6% (65 patients out of 68) noted 100% compliance in the use of the drug.Conclusions. The drug Dimia has a favorable profile of clinical and pharmacological safety and efficacy, along with the complete reversibility of contraceptive action when actualizing the issue of the realization of reproductive function. The advantages of the drug are to achieve well-studied non-contraceptive effects, in which patients with PCOS, internal endometriosis and uterine fibroids are interested, in the form of relieving manifestations of PMS and reducing the severity of dysmenorrhea, as well as cosmetic skin imperfections (acne-type rashes, hirsutism). Thus, the drug can be recommended to women of reproductive age as a reliable contraceptive, as well as to achieve non-contraceptive effects.

Cosmetic skin lightening: Contextualizing biomedical and ethical issues

The skin-lightening (SL) industry has a global reach and is projected to continue to grow over the coming decade. Although SL treatments may be safely prescribed for the treatment of some dermatologic conditions, many over-the-counter SL products contain ingredients that can cause harm to the skin and other organ systems. Given a lack of transparent information to patients and the historical colorist foundation that contextualizes a component of the cosmetic SL industry, dermatologists need to navigate biomedical and ethical concerns when explaining SL products to patients. This commentary briefly outlines the medical ethical issues surrounding this topic and describes avenues by which dermatologists may provide informed patient care that best supports beneficence, justice, autonomy, and nonmaleficence.

A hybrid sensory feedback system for thermal nociceptive warning and protection in prosthetic hand.

Advanced prosthetic hands may embed nanosensors and microelectronics in their cosmetic skin. Heat influx may cause damage to these delicate structures. Protecting the integrity of the prosthetic hand becomes critical and necessary to ensure sustainable function. This study aims to mimic the sensorimotor control strategy of the human hand in perceiving nociceptive stimuli and triggering self-protective mechanisms and to investigate how similar neuromorphic mechanisms implemented in prosthetic hand can allow amputees to both volitionally release a hot object upon a nociceptive warning and achieve reinforced release via a bionic withdrawal reflex. A steady-state temperature prediction algorithm was proposed to shorten the long response time of a thermosensitive temperature sensor. A hybrid sensory strategy for transmitting force and a nociceptive temperature warning using transcutaneous electrical nerve stimulation based on evoked tactile sensations was designed to reconstruct the nociceptive sensory loop for amputees. A bionic withdrawal reflex using neuromorphic muscle control technology was used so that the prosthetic hand reflexively opened when a harmful temperature was detected. Four able-bodied subjects and two forearm amputees randomly grasped a tube at the different temperatures based on these strategies. The average prediction error of temperature prediction algorithm was 8.30 ± 6.00%. The average success rate of six subjects in perceiving force and nociceptive temperature warnings was 86.90 and 94.30%, respectively. Under the reinforcement control mode in Test 2, the median reaction time of all subjects was 1.39 s, which was significantly faster than the median reaction time of 1.93 s in Test 1, in which two able-bodied subjects and two amputees participated. Results demonstrated the effectiveness of the integration of nociceptive sensory strategy and withdrawal reflex control strategy in a closed loop and also showed that amputees restored the warning of nociceptive sensation while also being able to withdraw from thermal danger through both voluntary and reflexive protection. This study demonstrated that it is feasible to restore the sensorimotor ability of amputees to warn and react against thermal nociceptive stimuli. Results further showed that the voluntary release and withdrawal reflex can work together to reinforce heat protection. Nevertheless, fusing voluntary and reflex functions for prosthetic performance in activities of daily living awaits a more cogent strategy in sensorimotor control.

Maize‐derived zein/acrylic hybrid emulsion for superior skin adhesion and water resistance in cosmetic adhesives

AbstractWaterborne acrylic emulsions are considered suitable materials for cosmetic skin adhesives because of their positive attributes, such as their safe water‐based polymerization process and low volatile‐organic‐compound emissions. The significant challenge of inadequate water resistance in emulsion adhesives, which is primarily caused by the excess surfactants retained at the interface of emulsion particles upon film formation, necessitates urgent resolution for their viable application as cosmetic adhesives. Zein, a corn‐derived protein, possesses hydrophobic amino acids that provide exceptional water‐repellent characteristics. Therefore, zein is expected to exhibit a remarkable skin‐adhesion attribute. This paper presents the introduction of a pioneering zein/acrylic hybrid emulsion exhibiting remarkable skin adhesion and water resistance, which was achieved through the use of zein, a corn‐derived protein. After formation, the zein/ sodium dodecyl sulfate (SDS) complex can unfold the zein chain, thereby enhancing skin adhesion, while concurrently inhibiting surfactant migration to enhance water resistance. The zein/SDS complex was introduced into the Pre‐emulsion (PE) manufacturing process during emulsion polymerization. As expected, the zein/acrylic hybrid emulsion demonstrated favorable skin adhesion and a notable improvement in water resistance. This approach presents a promising avenue for the practical application of emulsion nanoparticles as eco‐friendly cosmetic adhesives, offering excellent durability and skin adhesion through the utilization of naturally derived proteins.

Radio frequency hyperthermia system for skin tightening effect by filled waveguide aperture antenna with compact metamaterials.

Radio frequency (RF) hyperthermia focuses on raising the target area temperature to a value exceeding 45°C. Collagen is stimulated when the temperature rises to 45°C at the dermal layer, resulting in skin tightening. However, most studies on RF hyperthermia have focused on tumor ablation or using electrodes to radiate an electromagnetic field, which is highly inefficient. This study proposed a non-invasive RF hyperthermia skin-tightening system with a compact metamaterial-filled waveguide aperture antenna. The proposed RF system increased the temperature by 11.6°C and 35.3°C with 20 and 80W of 2.45GHz RF power, respectively, within 60s and exhibited a very focused effective area. Furthermore, a metamaterial was proposed to reduce the size of the waveguide aperture antenna and focus the electromagnetic field in the near-field region. The proposed metamaterial-filled waveguide aperture antenna was compact, measuring 10mm × 17.4mm, with a peak gain of 2.2dB at 2.45GHz. The measured hyperthermia performance indicated that the proposed RF system exhibited better power- and time-efficient hyperthermia performance than other RF hyperthermia systems in the cosmetic skin lifting commercial market. The proposed RF hyperthermia systems will be applied into a new generation of beauty cosmetic devices.

Study of Effect of Temperature on Commercial Moisturising Cream by UV-Visible Spectroscopy

Abstract: Skin moisturisation has become a part of our daily skin care routine whether the skin is dry or normal. To keep the skin hydrated moisturising creams are widely used across the world. Antioxidants are important ingredients in commercial cosmetic skin creams. It helps in extending the shelf life of a cream and preventing them from becoming rancid. α-Tocopherol acetate is a popular antioxidant in moisturising skin creams whether it is a herbal moisturising cream or a synthetic moisturising cream. The matrix of cosmetic creams is very complex due to the presence of several ingredients and therefore their extraction needs a special process. In this paper concentration of antioxidant in herbal and synthetic moisturizing creams have been calculated at various temperatures and at a specific time period by UV-Visible spectroscopy. This method is simple, sensitive, cost effective and reliable

Skin patch based makeup finish assessment technique by deep neural network.

Skin color and texture play a significant role in influencing impressions. To understand the influence of skin appearance and to develop better makeup products, objective evaluation methods for makeup finish have been explored. This study aims to apply machine learning technology, specifically deep neural network (DNN), to accurately analyze and evaluate delicate and complex cosmetic skin textures. "Skin patch datasets" were extracted from facial images and used to train a DNN model. The advantages of using skin patches include retaining fine texture, eliminating false correlations from non-skin features, and enabling visualization of the inferred results for the entire face. The DNN was trained in two ways: a classification task to classify skin attributes and a regression task to predict the visual assessment of experts. The trained DNNs were applied for the evaluation of actual makeup conditions. In the classification task training, skin patch-based classifiers for age range, presence or absence of base makeup, formulation type (powder/liquid) of the applied base makeup, and immediate/while after makeup application were developed. The trained DNNs on regression task showed high prediction accuracy for the experts' visual assessment. Application of DNN to the evaluation of actual makeup conditions clearly showed appropriate evaluation results in line with the appearance of the makeup finish. The proposed method of using DNNs trained on skin patches effectively evaluates makeup finish. This approach has potential applications in visual science research and cosmetics development. Further studies can explore the analysis of different skin conditions and the development of personalized cosmetics.

Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms.

The development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal peptidoglycan hydrolases, depolymerases, etc. Enzybiotic-based preparations are being studied for the treatment of various infections and, among others, can be used as topical formulations and dressings with protein-polysaccharide complexes. Here, we investigate the antibiofilm properties of a novel enzybiotic cocktail of phage endolysin LysSi3 and bacteriocin lysostaphin, formulated in the alginate gel matrix and its ability to control the opportunistic skin-colonizing bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, as well as mixed-species biofilms. Our results propose that the application of SiL-gel affects different components of biofilm extracellular polymeric substances, disrupts the matrix, and eliminates the bacteria embedded in it. This composition is highly effective against biofilms composed of Gram-negative and Gram-positive species and does not possess significant cytotoxic effects. Our data form the basis for the development of antibacterial skin care products with a gentle but effective mode of action.

Scientific Evidence for the Cosmetic Skin and Hair Benefits of Hemp-derived Cannabinoids: A Review

Cannabinoids derived from hemp, known as phytocannabinoids, are a group of approximately 120 bioactive compounds found in the Cannabis sativa plant. They have gained increasing attention in recent years, emerging as promising ingredients in the cosmetics industry due to their proven therapeutic and cosmetic benefits for skin and hair. It is worth noting that they do not produce psychoactive effects associated with tetrahydrocannabinol (THC). This paper summarizes the available published in vitro, in vivo and human studies on cosmetic benefits of cannabinoids for hair and skin. Several of the mechanisms of action of cannabinoids are novel and entirely different from all other cosmetic ingredients because they interact with specific receptors in the hair and skin cells to turn “on” or “off” cellular processes. In addition, there are issues related to the formulation, legal requirements and regulatory considerations associated with incorporating cannabinoids into cosmetics formulations as discussed.

A critical review investigating the use of nanoparticles in cosmetic skin products

This review article focuses on current cosmetic ingredients claiming a nano nature, the unique characteristics of nanomaterials and their behaviour on the skin, how they can be suitable for natural cosmetics and nanocosmetic environmental fate.

Функціональний стан шкіри в жінок із метаболічним синдромом

It is known that in people with metabolic diseases, reparation processes are disturbed during surgical interventions. However, the functional state of the skin during the development of the metabolic syndrome has been little studied. The aim of the work was to investigate the functional state of the skin in women with metabolic syndrome. Materials and methods. The study included 60 women aged 39 to 55 who sought surgical care for cosmetic skin defects. The control group consisted of 30 practically healthy women. Signs of metabolic syndrome were registered in 30 women who were included in the main group. The rate of reproduction of basal keratinocytes was determined according to the method of P. V. Kozhevnikova. The neutralizing ability of the skin was determined by the colorimetric method according to the time of disappearance of the bright crimson color (in minutes). The elastometric indicators of the skin were studied by the vacuum aspiration method using an applied cuvette with a diameter of 20 mm at a negative pressure of 20 kPa. The results. It was found that in women with metabolic syndrome, the duration of exfoliation of the stratum corneum increased by 48.1% (p < 0.01) compared to controls. This indicates a decrease in the rate of division of basal epidermocytes in the case of the development of metabolic syndrome. In the main group, the neutralization of the irritating agent was slower by 24.1% (p < 0.01), which indicates a decrease in the reactogenic properties of the skin in people with signs of metabolic syndrome. In patients with metabolic syndrome, the elastometric index of the skin increased by 46.0% (p < 0.001), which indicates changes in the tinctorial properties of the skin associated with the metabolic destruction of collagen. Conclusion. Significant changes in the functional state of the skin were registered in women with metabolic syndrome compared to healthy women: the rate of division of basal keratinocytes decreased, the elasticity of the skin deteriorated, and the index of its reactogenicity decreased. The identified changes, to a certain extent, explain the reasons for the violation of reparative processes in the skin after surgical interventions. Key words: metabolic syndrome, exfoliation of the stratum corneum of the skin, rate of division of basal keratinocytes, skin elasticity.

Clinico-epidemiology vitiligo profile in childhood at Mangusada General Hospital Badung

Introduction: Vitiligo is a depigmentation disorder characterized by well-demarcated white macules and patches on the skin. Vitiligo commonly occurs in children or young adults. Vitiligo can be classified into segmental, non-segmental and mixed type. Childhood vitiligo is often associated with many negative consequences, especially marked psychosocial and long-lasting effects on the self-esteem of the affected children. This study investigated characteristics and risk factors associated with vitiligo in childhood. Method: This was a retrospective descriptive study of patient aged 0-18 years old that was diagnosed with vitiligo derived from interview and medical records of Dermatovenereology Outpatient Clinic in Mangusada Hospital, Badung, Bali, from January to September 2023. This study included profiling data such as gender, the onset of vitiligo, clinical distribution, risk factors, and family history that suggested influencing the development of vitiligo. This study used a total sampling method, and 21 patients were participating. Results: Vitiligo in childhood was found in 11 patients female (52%) and 10 male (48%) patients in this study. A third of the patients were in elementary school, and only one (5,5%) was in preschool. The age of vitiligo patients was 13-15 (23,81%). The site of onset vitiligo was mostly in the face (21,42%), lower limb (17,85%), scalp, lumbar, and foot (10,71%). The distribution type focal distribution was predominant (38,09%), followed by segmental and generalized distribution (23,8%) and acrofacial (14,28%). Conclusions: Vitiligo was predominantly affected in a female patient. Idiopathic was the most common risk factor for vitiligo, and focal distribution was the most common type of lesion. Since vitiligo is a cosmetic skin problem and is not contagious or threatening the patient’s life, the available treatment is sufficient.

Rapid Colorimetric Detection of Hg (II) Based on Hg (II)-Induced Suppressed Enzyme-Like Reduction of 4-Nitrophenol by Au@ZnO/Fe3O4 in a Cosmetic Skin Product

Herein, we synthesized gold-coated ZnO/Fe3O4 nanocomposites. Initially, we prepared Fe3O4 magnetic nanoparticles based on the co-precipitation of Fe3+ and Fe2+ under aqueous ammonia as a precipitating agent. Thereafter, the ZnO/Fe3O4 composite was prepared by dispersing the synthesized magnetic nanoparticles into an alkaline zinc nitrate solution. After calcination of the precipitate, the formed ZnO/Fe3O4 composites were coated with gold nanostructures by dispersing the composites in auric acid/ethylene glycol solution in a water bath. The synthesized Au@ZnO/Fe3O4 hybrid material was able to catalyze the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP). We demonstrate that this catalytic activity can be exploited for the detection of Hg2+ ions in a cosmetic product. In the presence of Hg2+ ions, the catalytic activity of Au@ZnO/Fe3O4 was greatly suppressed. This novel finding underlies a straightforward, sensitive, and highly selective detection probe for Hg2+. The material exhibited excellent analytical performance as marked by the very low limit of detection (LOD) of 2.34 nM, which was well below acceptable levels of 4.99 μM for mercury in cosmetics as set by the US Food and Drug Administration (FDA), and within the linear dynamic ranges of 0–10 nM. High recoveries ranging from 96.5 to 100.3% accompanied by excellent selectivities toward Hg2+ over potentially interfering species were obtained.

Adalimumab Experience in the Management of Hidradenitis Suppurativa Burdened with Rapp-Hodgkin Syndrome

Background. Rapp-Hodgkin syndrome is a rare genetic disease from the ectodermal dysplasia group. It manifests with damage of ectodermal structures — layer of embrional tissue that provides the development of many organs and tissues in the body, such as: skin, sweat glands, hair, teeth, and nails. Disease symptoms may range from mild to severe and include dental abnormalities, fragile, thin or no hair, abnormal nails, hypohidrosis (due to the reduced number of sweat glands), cleft lips and palate. Hidradenitis suppurativa is a chronic inflammatory disease that predominantly affects apocrine glands areas. This nosology is characterized by painful nodes, abscesses, and fistulas, prone to relapses and leading to scarring.Clinical case description. The authors present a clinical case demonstrating the combination of Rapp-Hodgkin syndrome and severe hidradenitis suppurativa in 17-year-old male patient.Conclusion. The heterogeneous clinical findings of hidradenitis suppurativa and history of rare genetic disease result in patients' admissions to various medical specialists. Thus, it leads to delayed verification of the diagnosis and incorrect treatment methods. As a result, patients have persistent cosmetic skin defects, possible complications of irrational treatment, and decreased quality of life by the time of diagnosis.

LB1668 Anti-aging effects of autophagy analyzed within a 3D microfluidic skin-on-a-chip for skin cosmetics testing

LB1668 Anti-aging effects of autophagy analyzed within a 3D microfluidic skin-on-a-chip for skin cosmetics testing