Corynebacterium Glutamicum Biomass Research Articles

Performance, kinetics and equilibrium in biosorption of anionic dye Reactive Black 5 by the waste biomass of Corynebacterium glutamicum as a low-cost biosorbent

The protonated biomass of Corynebacterium glutamicum was used for the removal of Reactive Black 5 (RB 5). In batch experiments, the parameters studied included the effect of the dye concentration, pH, contact time, salt concentration and temperature. In the range of pH 1–3, the removal of RB 5 was kept at near 100%. The maximum uptakes estimated by using the Langmuir model were 169.5 and 185.2 mg/g at 20 and 40 ◦ C, respectively. Kinetic studies showed a pseudo second-order rate of biosorption with respect to the solution. The uptake of RB 5 was

Interaction between protonated waste biomass of Corynebacterium glutamicum and anionic dye Reactive Red 4

The protonated waste biomass of Corynebacterium glutamicum discharged from an industrial lysine fermentation plant was used as a new type of biosorbent for the removal of anionic dye Reactive Red 4. The dye uptake increased as the solution pH was decreased and at pH >7, the uptake was negligible. The maximum uptake of dye was estimated to be 104.6 ± 8.7 mg/g at pH 1. To identify the binding sites in the biomass, the biomass was potentiometrically titrated. As a result, three types of functional groups were present in the biomass, which was confirmed by FTIR analysis. Among functional groups, primary amine groups (–NH 2) were likely the binding sites for anionic Reactive Red 4. The proton dissociated constant p K H and molar content of the protonated amine groups were 9.14 ± 0.07 and 0.68 ± 0.02 mmol/g, respectively. It was also found that carboxyl and phosphonate groups played a role in electrostatic interference with the binding of dye molecules.

Biosorptive Decolorization of Reactive Orange 16 Using the Waste Biomass of Corynebacterium glutamicum

The protonated waste biomass of Corynebacterium glutamicum discharged from an industrial lysine fermentation plant was used for the removal of Reactive Orange 16 (RO16). The maximum sorption capacities of the biomass were as high as 186.6 ± 7.1 and 154.8 ± 2.8 mg g-1 at pH 1 and 2, respectively, which are comparable to those of commercial sorbents such as activated carbons and ion-exchange resins. As the solution pH decreased, the dye uptake increased and, under neutral conditions, it was negligible. Because the RO16 molecule has two negatively charged sulfonate groups, it is likely that the dye cannot be bound to a negatively charged carboxyl and/or phosphonate sites of the biomass. Instead, positively charged amine-occurring protein molecules seem to be dye binding sites. The uptake of RO16 was not significantly affected by a high concentration of salts, and the biomass could be repeatedly reused up to eight times per sorption/desorption cycle, reflecting that the biomass wastes deserve to be a potential regenerable biosorbent for dye removal.

Lead biosorption by waste biomass of Corynebacterium glutamicum generated from lysine fermentation process.

Biomass waste, mainly Corynebacterium glutamicum, is generated from large-scale lysine fermentation process. In this study, protonated C. glutamicum biomass was evaluated as a biosorbent for the removal of lead from synthetic wastewater. As Pb2+ were bound to the biomass, the solution pH deceased, indicating that protons in the biomass were exchanged with lead ions. The Corynebacterium biomass bound Pb2+ at up to 2.74 mmol g(-1) at pH 5, where lead does not precipitate. Compared with other biosorbents and conventional sorbents, such as natural zeolite, activated carbon and synthetic ion exchange resin, the protonated C. glutamicum biomass was considered to be a useful biomaterial for lead biosorption.