Accurate definition of the borders of cortical visual areas is essential for the study.of neuronal processes leading to perception. However, data used for definition of.areal boundaries have suffered from issues related to resolution, uniform.coverage, or suitability for objective analysis, leading to ambiguity. Here, we.present a novel approach that combines widefield optical imaging, presentation.of naturalistic movies, and encoding model analysis, to objectively define borders.in the primate extrastriate cortex. We applied this method to test conflicting.hypotheses about the third-tier visual cortex, where areal boundaries have.remained controversial. We demonstrate pronounced tuning preferences in the.third-tier areas, and an organizational structure in which the dorsomedial area.(DM) contains representations of both the upper and lower contralateral.quadrants, and is located immediate anterior to V2. High-density.electrophysiological recordings with a Neuropixels probe confirm these findings.Our encoding-model approach offers a powerful, objective way to disambiguate.areal boundaries…