You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (II)1 Apr 20131020 EFFECTS OF VARIOUS DURATIONS OF PRIAPISM ON CORPORAL OXIDATIVE STRESS PARAMETERS AND ANTIOXIDANT ENZYMES IN RAT MODEL Faruk Kucukdurmaz, Canan Kucukgergin, Oner Sanli, Tolga Akman, Sule Seckin, and Ates Kadioglu Faruk KucukdurmazFaruk Kucukdurmaz Gaziantep, Turkey More articles by this author , Canan KucukgerginCanan Kucukgergin Istanbul, Turkey More articles by this author , Oner SanliOner Sanli Istanbul, Turkey More articles by this author , Tolga AkmanTolga Akman Istanbul, Turkey More articles by this author , Sule SeckinSule Seckin Istanbul, Turkey More articles by this author , and Ates KadiogluAtes Kadioglu Istanbul, Turkey More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.605AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Ischemic priapism is characterized by progressive hypoxia,hypercapnia and acidosis which results with corporal necrosis.Previous studies reported decreased rate of erectile recovery after treatment of priapism longer than 36 hours. However,a recent study revealed that half of the patients with priapism longer than 72 hours achieved erectile recovery after surgery (T-shunt). Though, mechanism of corporal tissue resistance to ischemia still remained unclear. Our aim is to investigate the effect of varying durations of priapism on oxidative stress parameters and antioxidant enzymes in animal model of ischemic priapism. METHODS In this study, 24 Sprague-Dawley male rats were divided into four equal groups. Group 1 served as the control.Groups 2,3 and 4 represent 1,2 and 4 hours of priapism induced by vacuum erection device and a rubber band that was placed at the base of erect penis, respectively. After ½ hour of reperfusion,penectomy and blood withdrawal from heart were performed to investigate the levels of malondialdehyde (MDA),protein carbonyl (PC),superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GPX) RESULTS Corporal tissue MDA levels progressively increased with increased durations of priapism(p=0.01).In post hoc analysis, MDA in group 4 was significantly higher than group 1 and 2 (p<0.05).Corporal PC insignificantly increased according to duration of priapism (p=0.06).Corporal SOD significantly differed between group 1 and 4 (p=0.002) and between group 2 and 4(p=0.002). Also,there were significant differences in corporal GPX levels of group 1 and 4 (p=0.004) and group 2 and 4 (p=0.01).Since corporal CAT levels were higher in group 4,post hoc multivariable analysis did not reveal any significant differences between groups.Plasma MDA levels of group 2,3 and 4 were significantly higher than control group.Plasma GPX levels of group 3 and 4 were significanly higher than control group (p=0.0001; p=0.003).There were no significant differences among groups in terms of plasma CAT, SOD and PC levels. CONCLUSIONS Increase in antioxidant enzymes with increased duration of priapism may be related to resistance of corporal tissue to ischemic injury in the acute period.This may explain recovery of erectile function after treatment of prolonged priapism. Oxidative stress parameters and antioxidant enzyme levels in corporal tissue Group 1 Group 2 Group 3 Group 4 p value MDA(pmol/mg protein) (mean±SD;min-max) 3.52±0,77 (2.28-4.54) 4.29±0.55 (3.6-5.08) 5.29±1.52 (3.41-7.29) 6.36±1.10 (4.76-7.99) 0.01* PC (nmol/mg protein) (mean±SD;min-max) 2.37±0,45 (1.59-2.97) 3.38±1.02 (1.52-4.39) 3.87±1.65 (2.23-6.92) 4.16±1.02 (4.02-4.98) 0.06 GPX(nmol/mg protein) (mean±SD;min-max) 83.66±14.58 (67.2-102.5) 94.20±26.04 (80-125) 166.70±70.04 (85.5-256.8) 162.70±30.38 (136.2-198.1) 0.002* SOD(U/mg protein/ml) (mean±SD; min-max) 7.23±1.18 (5.56-8.36) 8.17±1.72 (7.83-10.23) 12.13±4.76 (8.63-17.52) 16.04±3.80 (11.74-20.82) 0.01* CAT(mM/mg protein) (mean±SD; min-max) 13.83±3.03 (9.79-17.27) 11.39±2.18 (8.19-13.73) 11.20±4.73 (4.87-18.22) 17.49±4.22 (10.96-22.44) 0.02# Post hoc multivariable analysis; *: between group 4 and group 1,2; #:no significant differences when compared between groups © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e418 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Faruk Kucukdurmaz Gaziantep, Turkey More articles by this author Canan Kucukgergin Istanbul, Turkey More articles by this author Oner Sanli Istanbul, Turkey More articles by this author Tolga Akman Istanbul, Turkey More articles by this author Sule Seckin Istanbul, Turkey More articles by this author Ates Kadioglu Istanbul, Turkey More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...