In recent years, the world has been facing severe challenges from climate change and environmental issues, with carbon dioxide emissions being considered one of the main driving factors. Many studies have proven that activities in various industries and fields have a significant impact on carbon dioxide emissions. However, few studies have explored the impact of gender on carbon dioxide emissions. This study aims to explore the potential impact of gender diversity on carbon dioxide emissions in the boards of directors of developed and emerging market enterprises. In addition, we also analyzed how board cultural diversity affects carbon dioxide emissions. We searched two European indices provided by Morgan Stanley Capital International (MSCI) from the Bloomberg database and conducted empirical analysis. We selected the MSCI index and MSCI emerging market index from 2010 to 2019 as samples and thoroughly cleaned up the data by removing any observations containing missing information on any variables. Statistical methods such as t-test, ordinary least squares, panel data analysis, regression analysis, and robustness testing were used for statistical analysis. At the same time, differential testing was conducted on sensitive and non-sensitive sectors, and the average representation of female boards in sensitive industries was low. The research results show that the proportion of female members on a company's board of directors is negatively correlated with carbon dioxide emissions. This discovery is consistent with the legitimacy theory advocating for gender equality and environmental sustainability, emphasizing the importance of gender diversity in reducing greenhouse gas emissions. However, agency theory suggests that diversity may lead to internal conflicts within a company, leading to agency costs and information asymmetry. The research results show a negative correlation between board cultural diversity and carbon dioxide emissions, indicating the potential challenge of board cultural diversity. This study provides important insights for decision-makers and managers, not only inspiring corporate social responsibility and environmental policy formulation, but also of great significance for academic research in the field of climate change. Our research findings help deepen our understanding of the factors that affect carbon dioxide emissions in different sectors and countries, while also expanding the research field between gender diversity, cultural diversity, and environmental sustainability. Although this study still needs to be further expanded and deepened, it provides useful insights into the relationship between board gender and cultural diversity and carbon dioxide emissions.