A field experiment was conduct in Agricultural Research Office fields / Al-Dibuni Research Station in Wasit-at spring season of 2020 in Clay Loam soil. The study aim effect of different fertilizers types in nitrogen, phosphorous, potassium concentrations and protein content in corn. A Factorial Experiment Design was used, including three levels of phosphorous fertilizer DAP (0, 25, 50 KgP2O5ha-1) representing levels P0 P1 P2, two levels of organic fertilizer were 0, 4 ton ha−1 representing M0, M1 and two levels of bio fertilizer which were B0, B1 respectively, According to the RCBD design with three replications. Corn seeds class 106 (Zea mays L.) sowed in plots (2*2m2) at amount of (120 Kg seeds ha−1). The Results showed that mineral, organic and bio fertilizers addition individually affected significantly of nitrogen, phosphorus and potassium concentration in dry matter, grains and protein content also, as the level P2, M1 and B1 was the best concentration compared to level P0, M0 and B0 respectively. Results showed the double interactions factors M1B1caused a significant increasing in nitrogen, phosphorous and potassium concentration in the dry matter, with increasing percentage 111.94, 161.53 and 85.44% in sequence compared with control treatment, and the same treatment M1B1 significantly affected the concentration of nitrogen and phosphorus in the grains, with increasing percentage 120.91 and 86.36% respectively, Comparison with control treatment, also noticed M1B1 treatment had a significant effect on protein content, with an increase percentage 121.46% compared to control treatment. Results showed the triple interactions P2M1B1 achieved affected significantly in nitrogen, phosphorus and potassium concentration in dry matter and grains, with increased percentage 142.86, 208.33 and 112.00%, respectively, compared with control treatment in dry matter, with increases of 145.33, 109.52 and 92.96% respectively compared with control treatment in grains. Results showed, that P2M1B1 treatment achieved affected significantly in protein content, with an increase percentage 144.68% compared to control treatment.