Core Complex Research Articles

Encapsulation of three different types of polyphenols in casein using a customized pH-driven method: Preparation and characterization

Phenolic compounds represent natural compounds endowed with diverse biological functionalities. However, their inherent limitations, characterized by poor water solubility and low oral bioavailability, limit their broader applications. Encapsulation delivery systems are emerging as a remedy, able to ameliorate these limitations by enhancing the stability and solubility of phenolic compounds. In this study, a novel, customized pH-driven approach was developed by determining the optimal deprotonation and protonation points of three different types of polyphenols: ferulic acid, resveratrol, and rhein. The polyphenols were successfully encapsulated in a casein carrier. The solubility, stability, LogD, and LogS curves of the three polyphenols at different pH values were analyzed to identify the optimal deprotonation points for ferulic acid (pH 9), resveratrol (pH 11), and rhein (pH 10). Based on these findings, three different nanoparticles were prepared. The encapsulation efficiencies of the three phenolic compounds were 95.86%, 94.62%, and 94.18%, respectively, and the casein nanoparticles remained stable at room temperature for seven days. FTIR spectroscopy, fluorescence spectroscopy, and molecular docking study substantiated the encapsulation of phenolic compounds within the hydrophobic core of casein-based complexes, facilitated by hydrogen bonding interactions and hydrophobic interactions. Furthermore, the analysis of antioxidant activity elucidated that casein nanoparticles heightened both the water solubility and antioxidant efficacy of the phenolic compounds. This customized encapsulation technique, by establishing a transitional pH value, resolves the challenges of chemical instability and facile degradation of polyphenols under alkaline conditions in the application process of pH-driven methods. It presents novel insights for the application of polyphenols in the domains of food and biomedical fields.

From toxic bonds to healing relationships. A journey through group analytic theory and practice

The essentials of therapeutic practice in group analysis are summarised through three basic principles. The conductor is responsible for fostering a climate that allows each of a group’s members to find their own voice; bear witness to the voices of others; and come out of the shadows together through the group’s discourse. These principles are built on the author’s earlier writing about the speech, silence, and the language of the group which can make a home amongst strangers. They are framed in the ordinary language of daily experience and the parallel idiom of musicology to address the most primary of musical instruments—the voice. They incorporate of a range of other terms and concepts that bring our attention to core complexities at the heart of many peoples’ deepest sources of disquiet arising out of unresolved intergenerational trauma. The group’s work is akin to a mourning process set in motion for hitherto unacknowledged grief. Disturbance can move about unpredictably. Our challenge is to locate and translate hidden, buried or displaced injury into the everyday language of human suffering where seeds can be sown for resolution. This multi-modal presentation is illustrated with clinical vignettes from groups at work and with collage and paintings. It opens with a Paul McCartney song, ‘Blackbird’ (1968), whose music and lyrics give historical significance to the struggle for human rights. Its closing vignettes explore ‘tools for conviviality’ with images of lost and found brotherhood and a spontaneous online ceremony of eating together in China. The paintings of Morris Nitsun bring us to an ultimate agency for change generated by small groups—the shifting mood-scape of their dynamic life. One image conveys the tragedy and desolation of the pandemic, and another offers the natural world’s consolation in beauty.

Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.

Enhancing the efficiency of photosynthesis represents a promising strategy to improve crop yields, with keeping the steady state of PSII being key to determining the photosynthetic performance. However, the mechanisms whereby the stability of PSII is maintained in oxygenic organisms remain to be explored. Here, we report that the Psb28 protein functions in regulating the homeostasis of PSII under different light conditions in Arabidopsis thaliana. The psb28 mutant is much smaller than the wild-type plants under normal growth light, which is due to its significantly reduced PSII activity. Similar defects were seen under low light and became more pronounced under photoinhibitory light. Notably, the amounts of PSII core complexes and core subunits are specifically decreased in psb28, whereas the abundance of other representative components of photosynthetic complexes remains largely unaltered. Although the PSII activity of psb28 was severely reduced when subjected to high light, its recovery from photoinactivation was not affected. By contrast, the degradation of PSII core protein subunits is dramatically accelerated in the presence of lincomycin. These results indicate that psb28 is defective in the photoprotection of PSII, which is consistent with the observation that the overall NPQ is much lower in psb28 compared to the wild type. Moreover, the Psb28 protein is associated with PSII core complexes and interacts mainly with the CP47 subunit of PSII core. Taken together, these findings reveal an important role for Psb28 in the protection and stabilization of PSII core in response to changes in light environments.

Ultrafast Energy Transfer in a Diatom Photosystem II Supercomplex.

The light-harvesting complexes (LHCs) of diatoms, specifically fucoxanthin-Chl a/c binding proteins (FCPs), exhibit structural and functional diversity, as highlighted by recent structural studies of photosystem II-FCP (PSII-FCPII) supercomplexes from different diatom species. The excitation dynamics of PSII-FCPII supercomplexes isolated from the diatom Thalassiosira pseudonana was explored using time-resolved fluorescence spectroscopy and two-dimensional electronic spectroscopy at room temperature and 77 K. Energy transfer between FCPII and PSII occurred remarkably fast (<5 ps), emphasizing the efficiency of FCPII as a light-harvesting antenna. The presence of long-wavelength chlorophylls may further help concentrate excitations in the core complex and increase the efficiency of light harvesting. Structure-based calculations reveal remarkably strong excitonic couplings between chlorophylls in the FCP antenna and between FCP and the PSII core antenna that are the basis for the rapid energy transfer.

Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro.

Eukaryotic cells tether the nucleoskeleton to the cytoskeleton via a conserved molecular bridge, called the LINC complex. The core of the LINC complex comprises SUN-domain and KASH-domain proteins that directly associate within the nuclear envelope lumen. Intra- and inter-chain disulphide bonds, along with KASH-domain protein interactions, both contribute to the tertiary and quaternary structure of vertebrate SUN-domain proteins. The significance of these bonds and the role of PDIs (protein disulphide isomerases) in LINC complex biology remains unclear. Reducing and non-reducing SDS-PAGE analyses revealed a prevalence of SUN2 homodimers in non-tumorigenic breast epithelia MCF10A cells, but not in the invasive triple-negative breast cancer MDA-MB-231 cell line. Furthermore, super-resolution microscopy revealed SUN2 staining alterations in MCF10A, but not in MDA-MB-231 nuclei, upon reducing agent exposure. While PDIA1 levels were similar in both cell lines, pharmacological inhibition of PDI activity in MDA-MB-231 cells led to SUN-domain protein down-regulation, as well as Nesprin-2 displacement from the nucleus. This inhibition also caused changes in perinuclear cytoskeletal architecture and lamin downregulation, and increased the invasiveness of PDI-inhibited MDA-MB-231 cells in space-restrictive in vitro environments, compared to untreated cells. These results emphasise the key roles of PDIs in regulating LINC complex biology, cellular architecture, biomechanics, and invasion.

Work Motivation and Work Enthusiams of Madrasah Muallimin Muhammadiyah Yogyakarta Teachers

Teachers at Madrasah Muallimin Muhammadiyah Yogyakarta have complex and diverse core tasks and functions that affect motivation and work spirit. The purpose of this research is to know the motivation of work and the work spirit of teachers at Madrasah Muallimin Muhammadiyah Yogyakarta. The study uses qualitative design through in-depth interviews and direct observation of 4 (four) teachers with non-permanent teacher status. Testing the credibility of data with member checking is by confirming the informant that the informants agree with the data found. The data analysis process is carried out through the stages of data collection, data reduction, data presentation, and data verification. The results of the research show that the spirit of work arises from the desire to accomplish this, which is reinforced by the presence of reasonable compensation. The need for affiliation becomes an important thing for teachers to become comfortable and enthusiastic at work. Maintaining harmony between teachers and having friends will have an impact on comfort and increased productivity at work. Great power brings great responsibility, and as a teacher grows competent, it will automatically affect greater responsibility.

Botanical and ecological characteristics of the floristic core of woody-shrub plant communities on technogenically devastated lands (Kryvyi Rih, Ukraine)

The long-term effect of man-made impact on the environment leads to a radical change in the biogeocenotic cover, among which a special place is given to the devased lands of the Kryvyi Rih region. Their area has increased significantly over the past fifty years and continues to grow and today is more than 30 thousand hectares. hectare. The process of restoring these lands, in particular the quarry-dump type, which were formed during open-pit mining, is usually slow, the natural vegetation cover here quite specific. The present study examines the results of assessing the floristic composition, ecomorphic characteristics, and vitality of woody and shrub plants that constitute the floristic core on technogenically devastated lands. The study utilized the results of our own research conducted from 2019 to 2023 on the territory of the disturbed areas in the Kryvyi Rih iron ore region. It has been determined that the floristic core on the devastated lands of the Kryvyi Rih region consists of 19 species belonging to 15 genera and 11 families. Within the ecological spectrum of the floristic complex (core) comprising woody and shrubby species, mesoxerophytes are prevalent, succeeded by mesotrophs, with a consistent occurrence of sciophytes and heliophytes. Our results indicate that the ecological conditions of the devastated lands are relatively favorable for the growth and development of woody and shrub plant species. It was found that the vital condition of the floristic complex (core) was assessed as «weakened». The condition of woody plants suggests the relative favorability of the ecological conditions of the dump for their growth and development.

Autonomous Feedback-Driven Engineered DNAzyme-Coated Trojan Horse-like Nanocapsules for On-Demand CRISPR/Cas9 Delivery.

Manipulating the expression of cellular genes through efficient CRISPR/Cas9 delivery is rapidly evolving into a desirable tumor therapeutics. The exposure of CRISPR/Cas9 to a complex external environment poses challenges for conventional delivery carriers in achieving responsive and accurate release. Here, we report a Trojan horse-like nanocapsule for the on-demand delivery of CRISPR/Cas9 in a microRNA-responsive manner, enabling precise tumor therapy. The nanocapsule comprises a nanoassembled, engineered DNAzyme shell encasing a Cas9/sgRNA complex core. The DNAzyme, functioning as a catalytic unit, undergoes a conformational change in the presence of tumor-associated microRNA, followed by activating a positive feedback-driven autonomous catabolic cycle of the nanocapsule shell. This catabolic cycle is accomplished through chain reactions of DNAzyme "cleavage-hybridization-cleavage", which ensures sensitivity in microRNA recognition and effective release of Cas9/sgRNA. Utilizing this Trojan horse-like nanocapsule, as low as 1.7 pM microRNA-21 can trigger the on-demand release of Cas9/sgRNA, enabling the specific editing of the protumorigenic microRNA coding gene. The resulting upregulation of tumor suppressor genes induces apoptosis in tumor cells, leading to significant inhibition of tumor growth by up to 75.94%. The Trojan horse-like nanocapsule, with superior programmability and biocompatibility, is anticipated to serve as a promising carrier for tailoring responsive gene editing systems, achieving enhanced antitumor specificity and efficacy.

Inhomogeneous energy transfer dynamics from iron-stress-induced protein A to photosystem I.

Cyanobacteria respond to iron limitation by producing the pigment-protein complex IsiA, forming rings associated with photosystem I (PSI). Initially considered a chlorophyll-storage protein, IsiA is known to act as an auxiliary light-harvesting antenna of PSI, increasing its absorption cross-section and reducing the need for iron-rich PSI core complexes. Spectroscopic studies have demonstrated efficient energy transfer from IsiA to PSI. Here we investigate the room-temperature excitation dynamics in isolated PSI-IsiA, PSI, IsiA monomer complexes and IsiA aggregates using two-dimensional electronic spectroscopy. Cross analyses of the data from these three samples allow us to resolve components of energy transfer between IsiA and PSI with lifetimes of 2-3 ps and around 20 ps. Structure-based Förster theory calculations predict a single major timescale of IsiA-PSI equilibration, that depends on multiple energy transfer routes between different IsiA subunits in the ring. Despite the experimentally observed lifetime heterogeneity, which is attributed to structural heterogeneity of the supercomplexes, IsiA is found to be a unique, highly efficient, membrane antenna complex in cyanobacteria.

Correction: Subdomains of the Helicobacter pylori Cag T4SS outer membrane core complex exhibit structural independence.

Correction: Subdomains of the Helicobacter pylori Cag T4SS outer membrane core complex exhibit structural independence.

Unveiling the connection between lode gold mineralization and the metamorphic core complex evolution from the large Yangzhaiyu gold deposit, North China Craton

Abstract The Xiaoqinling gold province, located in the Neoarchean–Paleoproterozoic uplifted footwalls of the Xiaoqinling metamorphic core complex (XMCC), is one of China’s largest gold producers; however, achieving a consensus regarding their metallogenic model remains elusive. Scheelite is an indicator mineral that commonly occurs in lode gold deposits worldwide used to recognize deposit types and understand hydrothermal evolution and the origin of features. Xenotime, monazite, and rutile are common hydrothermal minerals in association with lode gold deposits worldwide. Here, we provide textual, in situ U-Pb geochronology of xenotime, monazite, and rutile, and in situ elemental and Sr-Nd isotopic compositions of scheelite within different stages from the large Yangzhaiyu lode gold deposit, aiming to elucidate its genesis and, for the first time, establish a holistic correlation between the lode gold mineralization and the evolution of the XMCC. Notably low εNd(t) values (−30.7 to −23.7), high 87Sr/86Sr ratios (0.72659–0.75914), and distinct rare earth elements, Sr, Mo, and As contents of scheelite confirm a metamorphic crustal source. Xenotime U-Pb dating and pre-ore (Stage I) scheelite reveal that ore-barren metamorphic fluids at ca. 140 Ma were oxidized with low Bi contents and buffered by greenschist facies metamorphism when the XMCC initiated. Monazite and rutile U-Pb dating combined with ore-stage scheelite geochemistry indicate a compositional shift in the more reduced auriferous metamorphic fluids, which dominated during major gold deposition periods (stages II and III) from 130 Ma to 120 Ma, characterized by significantly depleted Na and increased Bi contents. This resulted from the prograde greenschist-to-amphibolite metamorphism at mid-lower crustal depths as the result of the XMCC isostatic doming and the lithospheric mantle thinning after 130 Ma. This study highlights the crucial role of metamorphic core complexes in governing the timing, locations, and resources of the lode gold metallogenic system.

Investigating the Balance between Structural Conservation and Functional Flexibility in Photosystem I.

Photosynthesis, as the primary source of energy for all life forms, plays a crucial role in maintaining the global balance of energy, entropy, and enthalpy in living organisms. Among its various building blocks, photosystem I (PSI) is responsible for light-driven electron transfer, crucial for generating cellular reducing power. PSI acts as a light-driven plastocyanin-ferredoxin oxidoreductase and is situated in the thylakoid membranes of cyanobacteria and the chloroplasts of eukaryotic photosynthetic organisms. Comprehending the structure and function of the photosynthetic machinery is essential for understanding its mode of action. New insights are offered into the structure and function of PSI and its associated light-harvesting proteins, with a specific focus on the remarkable structural conservation of the core complex and high plasticity of the peripheral light-harvesting complexes.

Hydrothermal flow and serpentinization in oceanic core complexes controlled by mafic intrusions

Hydrothermal flow and serpentinization in oceanic core complexes controlled by mafic intrusions

Molecular structure and characterization of the Thermochromatium tepidum light-harvesting 1 photocomplex produced in a foreign host

Purple phototrophic bacteria possess light-harvesting 1 and reaction center (LH1-RC) core complexes that play a key role in converting solar energy to chemical energy. High-resolution structures of LH1-RC and RC complexes have been intensively studied and have yielded critical insight into the architecture and interactions of their proteins, pigments, and cofactors. Nevertheless, a detailed picture of the structure and assembly of LH1-only complexes is lacking due to the intimate association between LH1 and the RC. To study the intrinsic properties and structure of an LH1-only complex, a genetic system was constructed to express the Thermochromatium (Tch.) tepidum LH1 complex heterologously in a modified Rhodospirillum rubrum mutant strain. The heterologously expressed Tch. tepidum LH1 complex was isolated in a pure form free of the RC and exhibited the characteristic absorption properties of Tch. tepidum. Cryo-EM structures of the LH1-only complexes revealed a closed circular ring consisting of either 14 or 15 αβ-subunits, making it the smallest completely closed LH1 complex discovered thus far. Surprisingly, the Tch. tepidum LH1-only complex displayed even higher thermostability than that of the native LH1-RC complex. These results reveal previously unsuspected plasticity of the LH1 complex, provide new insights into the structure and assembly of the LH1-RC complex, and show how molecular genetics can be exploited to study membrane proteins from phototrophic organisms whose genetic manipulation is not yet possible.

Knockdown of the SDHB Subunit Induces Mitochondrial Remodeling in Human Airway Smooth Muscle Cells

Succinate dehydrogenase (SDH) is a mitochondrial enzyme that serves a dual role in mitochondrial respiration: catalyzing the oxidation of succinate to fumarate in the tricarboxylic acid (TCA) cycle and transferring electrons from succinate to ubiquinone as complex II in the electron transport chain (ETC). SDH is composed of four subunits (SDHA-SDHD) and the loss of SDHB subunit, specifically, has been shown to increase oxidative stress. As a part of the catalytic core of the SDH complex, the SDHB subunit mediates electron transfer to ubiquinone. In human airway smooth muscle (hASM) cells with siRNA-mediated knockdown of SDHB, the maximum velocity of the SDH reaction (SDHmax) is decreased. Moreover, SDHB-knockdown leads to an increase in mitochondrial volume density, indicating a role of SDH in mitochondrial remodeling. However, the impact of SDHB-knockdown on mitochondrial dynamics has not been explored. Therefore, we hypothesize that SDHB-knockdown promotes mitochondrial fragmentation as part of mitochondrial remodeling in hASM cells. To investigate this hypothesis, bronchiolar tissue samples were collected in female and male patients undergoing lung surgery with no current history of smoking or respiratory diseases, the smooth muscle layer was dissected and hASM cells were dissociated. The targeted knockdown of SDHB was achieved by siRNA-mediated knockdown. Mitochondria in hASM cells were labeled with MitoTracker Green (200 nM) and imaged in 3D with a Z optical slice of 0.5 μm using an oil-immersion ×60/1.4 NA objective on a Nikon Eclipse A1 laser scanning confocal system. Optical slices were deconvolved to enhance contrast and the MitoTracker labeling was thresholded to create binary images, which were then reconstructed in 3D. From these images, the mitochondrial complexity index (MCI) was calculated to quantify the extent of fragmentation. To identify the signaling pathway involved in mitochondrial fragmentation, the expression and phosphorylation of dynamin-related protein 1 (DRP1) was quantified by Western blot. Mitochondrial fragmentation is associated with increased phosphorylation of DRP1 at Ser616 (pDRP1s616). Furthermore, mitophagy was assessed by determining the mitochondrial expression of PINK1 and the phosphorylation of Parkin at Ser65 (pParkinS65). Mitochondrial biogenesis was also evaluated through the expression of PGC1α, NRF1/2 and TFAM. Our results show that SDHB-knockdown increased mitochondrial fragmentation (reduced MCI) in hASM cells, and increased pDRP1s616 phosphorylation and its translocation to mitochondria. SDHB-knockdown also increased mitochondrial expression of PINK1 and pParkins65 phosphorylation indicating activation of mitophagy. We also confirmed that SDHB-knockdown increased expression of PGC1α, NRF1/2 and TFAM consistent with mitochondrial biogenesis. These findings unveil the mechanisms underlying SDHB-knockdown-mediated mitochondrial remodeling. Supported by NIH grant R01-HL157984 (GCS). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

The power of modern 3-D visualization of high-resolution terrain models in geologic mapping: Complex fold geometries revealed by 3-D mapping in the Panamint metamorphic complex, eastern California, USA

We use structure from motion–multiview stereo (SM) terrain models developed from ground-based images and images acquired from uncrewed aircraft (aka drones) as a base map for three-dimensional (3-D) mapping on the walls of a deep canyon in the Panamint Range of eastern California, USA. The ability to manipulate the 3-D model with views from arbitrary look directions and broad scale range revealed structures that were invisible to conventional two-dimensional (2-D) mapping because of both the scale of the structures and their exposure on vertical to near-vertical cliff faces. The analysis supports field evidence for four phases of ductile deformation, with only one of the younger phases documented on early geologic maps of the area. The oldest deformational event (D1) produced the main metamorphic fabric and pre-dates Late Cretaceous plutons. This deformation produced a 200–250-m-thick high-strain zone localized along marbles at the top of the Kingston Peak Formation and lower Noonday Formation. Geometric analysis from the model suggests strongly that large sheath folds at scales of 100–300 m are developed within these marbles. Large measured finite strains indicate displacement across this apparent shear zone of at least 4–5 km and displacements of tens of kilometers are allowable, yet the structure is invisible to conventional mapping because the high-strain zone is stratabound. The main fabric shows two clear overprints and a third that is likely an even younger deformation. D2 and D3 generated tight to close, recumbent folds and open to tight, upright folds, respectively, both folding the main foliation with localized development of crenulation cleavages axial planar to the folds. An additional overprint shows no clear cross-cutting relationship with D2 or D3 fabrics and could be a manifestation of either of those events, although the deformation is spatially limited to a narrow shear zone beneath a brittle, dextral-normal fault with the same kinematics as a mylonitic fabric in a Cretaceous granite in the footwall. This observation suggests an extensional, core complex–style deformation to produce this structure. We suggest that 3-D mapping has the potential to revolutionize geologic mapping studies, particularly where steep topography provides 3-D views that are virtually invisible on conventional 2-D maps. Previously bewildering geologic puzzles can be solved by the ability to visualize large cliff exposures from arbitrary angles and map the features in true 3-D at resolutions to the centimeter level. Although this study emphasized intermediate scales imaged by a drone, our methods here are easily extended to larger scales using a crewed aircraft for imaging. We suggest these methods should be used routinely in frontier areas with steep terrain where aviation is already in use for access, but the methods can be employed anywhere steep terrain “hides” major rock exposures on conventional 2-D maps.

Local earthquake tomography of the Aegean crust: Implications for active deformation, large earthquakes, and arc volcanism

Three-dimensional velocity models can enable accurate earthquake location, improved seismic hazard assessment, and can enhance our understanding of geodynamic processes. This is particularly true for areas such as the Aegean, where the crust is marked by active volcanoes as well as shear and rift zones, all of which make this region highly heterogeneous. This work describes the application of local earthquake tomography in order to elucidate the crustal structure of the Aegean. We utilized a dataset of 2,135,625 P arrivals and 1,095,515 S arrivals from 178,676 events recorded across 324 stations. Results indicate that the boundary separating the slow Vp from the fast anomalies at 20–40 km depth is well correlated with the Aegean Moho obtained from receiver functions. A slow Vp (−5%) zone at depths >30 km along the Hellenic arc is likely associated with basal underplating that forms the base of the crust. On the other hand, fast Vp and moderate to high Vp/Vs (1.77–1.92) in the upper-middle crust may indicate evolving metamorphic core complexes. Steeply dipping clusters of earthquakes could highlight pathways of fluid migration from the base to the middle/upper crust, albeit more detailed seismological studies are needed to confirm this interpretation. The 3D velocity model can be also utilized in order to investigate the amount of melt fraction beneath active volcanoes and the influence of fluids on the rupture zone of large earthquakes. Our results show a melt fraction between 4 and 10% beneath active volcanoes, with the largest volume of melt present beneath Santorini caldera. The ruptures zones of 10 large earthquakes (Mw ≥ 6.0), show slow Vp anomalies and moderate to high Vp/Vs (> 1.77), suggesting that these events were likely triggered by weakening of their source zones by fluid activity.

New insights into the structure and dynamics of the TOM complex in mitochondria.

To date, there is no general physical model of the mechanism by which unfolded polypeptide chains with different properties are imported into the mitochondria. At the molecular level, it is still unclear how transit polypeptides approach, are captured by the protein translocation machinery in the outer mitochondrial membrane, and how they subsequently cross the entropic barrier of a protein translocation pore to enter the intermembrane space. This deficiency has been due to the lack of detailed structural and dynamic information about the membrane pores. In this review, we focus on the recently determined sub-nanometer cryo-EM structures and our current knowledge of the dynamics of the mitochondrial two-pore outer membrane protein translocation machinery (TOM core complex), which provide a starting point for addressing the above questions. Of particular interest are recent discoveries showing that the TOM core complex can act as a mechanosensor, where the pores close as a result of interaction with membrane-proximal structures. We highlight unusual and new correlations between the structural elements of the TOM complexes and their dynamic behavior in the membrane environment.

Subdomains of the Helicobacter pylori Cag T4SS outer membrane core complex exhibit structural independence.

The Helicobacter pylori Cag type IV secretion system (Cag T4SS) has an important role in the pathogenesis of gastric cancer. The Cag T4SS outer membrane core complex (OMCC) is organized into three regions: a 14-fold symmetric outer membrane cap (OMC) composed of CagY, CagX, CagT, CagM, and Cag3; a 17-fold symmetric periplasmic ring (PR) composed of CagY and CagX; and a stalk with unknown composition. We investigated how CagT, CagM, and a conserved antenna projection (AP) region of CagY contribute to the structural organization of the OMCC. Single-particle cryo-EM analyses showed that complexes purified from ΔcagT or ΔcagM mutants no longer had organized OMCs, but the PRs remained structured. OMCCs purified from a CagY antenna projection mutant (CagY∆AP) were structurally similar to WT OMCCs, except for the absence of the α-helical antenna projection. These results indicate that CagY and CagX are sufficient for maintaining a stable PR, but the organization of the OMC requires CagY, CagX, CagM, and CagT. Our results highlight an unexpected structural independence of two major subdomains of the Cag T4SS OMCC.

Magma intrusion and migration under the continental large igneous provinces

Large igneous provinces (LIPs) are often the surface expressions of mantle plume process. Intensive magma could occur in the continental crust when a mantle plume penetrates the Moho. How magmas migrate in the crust, forming LIPs, remains debated. In this study, we employ 2D thermo‐mechanical numerical modelling to study the dynamics of magma intrusion and migration within the continental crust affected by a mantle plume. Our results suggest that (1) lateral magma migration dominates crustal deformation, promoting the possible formation of metamorphic core complexes; (2) two distinct crustal deformation modes are recognized regarding the presence or absence of crustal break‐up, affected by whether a significant vertical magma migration occurs; (3) the crustal thickness, Moho temperature and the length of the preset weak crust–mantle decoupling zone are the three key parameters controlling crustal magma migration. This study highlights the importance of lateral magma migration in the lower crust and provides physical mechanisms for the interpretation of magma migration under LIPs.