The innovation environment is the basis for the operation of the innovation ecosystem. In an era where knowledge resources are increasingly recognized as core assets, the advent of transactive memory system (TMS) has significantly enhanced the knowledge collaboration among R&D (Research & Development) teams, thereby boosting their innovation performance. Based on the empirical analysis of 235 valid questionnaires, this paper explores the relationship among the innovation environment, R&D Team's TMS, and knowledge collaboration. The results show that: (1) the Innovation environment has a direct and significant positive effect on the team's TMS and knowledge collaboration; (2) Team's TMS has some direct and significant positive effects and mediating effects on Knowledge Collaboration, and the Coordination of team's TMS has no significant effect on the Knowledge Integration process of Knowledge Collaboration; (3) The innovation environment can indirectly affect the knowledge collaboration process through the Specialization and Credibility of the TMS. This study further enriches the relevant research in the field of knowledge management and provides theoretical support and countermeasures for enterprises to promote innovation and development by optimizing knowledge management.