Coral Research Articles

A comparative study of surface modified nanohydroxyapatite using PVA polymer extracted from Seashells, Coral Skeletons and Eggshells for biomedical applications

The necessity and usage of hydroxyapatite in the form of bone and dental substitutes are increasing day by day. Hydroxyapatite derived from natural biomaterials promotes better osteointegration and biocompatibility with host bone. The present investigation aims to compare the properties of three distinct naturally occurring calcium precursors (seashells, coral skeletons, and eggshells) derived from nHAp/PVA composites (Polymeric composites). All the prepared samples were examined through characteristic instruments such as XRD (X-ray diffraction), FT-IR (Fourier Transform Infrared) Spectroscopy, TG-DTA (Thermogravimetric-Differential Thermal Analysis), SEM/EDAX (Scanning Electron Microscopy/Energy Dispersive X-Ray Spectroscopy), and XPS (X-Ray Photoelectron Spectroscopy). Furthermore, the samples were subjected to the Simulated Body Fluid (SBF), Hemocompatibility and Antimicrobial assay to find the Bioactivity, Hemocompatible and Antibacterial efficiency of the samples. The surface morphology of seashell nHAp/PVA shows a spherical shape while coral and eggshell-derived nHAp/PVA exhibits rod-shaped morphology. However, the achieved particle is found to be in the range of 32 to 41 nm. Also, coral-derived nHAp/PVA composites show a maximum crystallinity of 83.7% with a minimum weight loss of about 49.96%. This study demonstrates that among the prepared samples, coral nHAp/PVA performs better, but in terms of availability, seashell/PVA takes the lead with good results in all aspects. Though the eggshell nHAp/PVA satisfies several characteristic studies, it shows less stableness on TG-DTA. This study suggests that both Seashells and Corals can be utilized as calcium precursors in the making of quality hydroxyapatite that can be further processed in the making of hard tissue replacements/substitutes.

Newly deceased Caribbean reef-building corals experience rapid carbonate loss and colonization by endolithic organisms

Coral mortality triggers the loss of carbonates fixed within coral skeletons, compromising the reef matrix. Here, we estimate rates of carbonate loss in newly deceased colonies of four Caribbean reef-building corals. We use samples from living and recently deceased colonies following a stony coral tissue loss disease (SCTLD) outbreak. Optical densitometry and porosity analyses reveal a loss of up to 40% of the calcium carbonate (CaCO3) content in dead colonies. The metabolic activity of the endolithic organisms colonizing the dead skeletons is likely partially responsible for the observed dissolution. To test for the consequences of mass mortality events over larger spatial scales, we integrate our estimates of carbonate loss with field data of the composition and size structure of coral communities. The dissolution rate depends on the relative abundance of coral species and the structural properties of their skeletons, yet we estimate an average reduction of 1.33 kg CaCO3 m−2, nearly 7% of the total amount of CaCO3 sequestered in the entire system. Our findings highlight the importance of including biological and chemical processes of CaCO3 dissolution in reef carbonate budgets, particularly as the impacts of global warming, ocean acidification, and disease likely enhance dissolution processes.

Microorganisms and dissolved metabolites distinguish Florida's Coral Reef habitats.

As coral reef ecosystems experience unprecedented change, effective monitoring of reef features supports management, conservation, and intervention efforts. Omic techniques show promise in quantifying key components of reef ecosystems including dissolved metabolites and microorganisms that may serve as invisible sensors for reef ecosystem dynamics. Dissolved metabolites are released by reef organisms and transferred among microorganisms, acting as chemical currencies and contributing to nutrient cycling and signaling on reefs. Here, we applied four omic techniques (taxonomic microbiome via amplicon sequencing, functional microbiome via shotgun metagenomics, targeted metabolomics, and untargeted metabolomics) to waters overlying Florida's Coral Reef, as well as microbiome profiling on individual coral colonies from these reefs to understand how microbes and dissolved metabolites reflect biogeographical, benthic, and nutrient properties of this 500-km barrier reef. We show that the microbial and metabolite omic approaches each differentiated reef habitats based on geographic zone. Further, seawater microbiome profiling and targeted metabolomics were significantly related to more reef habitat characteristics, such as amount of hard and soft coral, compared to metagenomic sequencing and untargeted metabolomics. Across five coral species, microbiomes were also significantly related to reef zone, followed by species and disease status, suggesting that the geographic water circulation patterns in Florida also impact the microbiomes of reef builders. A combination of differential abundance and indicator species analyses revealed metabolite and microbial signatures of specific reef zones, which demonstrates the utility of these techniques to provide new insights into reef microbial and metabolite features that reflect broader ecosystem processes.

Coral Detection, Ranging, and Assessment (CDRA) algorithm-based automatic estimation of coral reef coverage

Climate change poses significant threats to the health of coral reefs. The detection of coral coverage is a quantitative method for assessing the health of coral reefs. Valuable insights into the effects of climate change on coral reef ecosystems and the trends in coral reef development can be gained through the monitoring of coral coverage. However, traditional manual methods for estimating coral coverage are time-consuming and labor-intensive, limiting the scalability and efficiency of coral surveys. The field of automated processing for coral video imagery is currently limited, impeding the development of effective techniques for comprehensive coral monitoring and further hindering progress in coral ecosystem monitoring. To cope with these challenges, there is a crucial need for automated systems to efficiently handle large volumes of video data, thereby transforming imaging devices, such as underwater robots, into autonomous sensors capable of conducting reliable and comprehensive surveys. In response to this pressing issue, this study presents a novel video analysis approach, termed the Coral Detection, Ranging, and Assessment (CDRA) algorithm, which combines cutting-edge computer vision techniques including You Only Look Once version 5 (YOLOv5) object detection, binocular stereo vision, and tracking algorithms. The CDRA algorithm aims to analyze continuous video segments of coral reefs, enabling the identification, counting, and estimation of size and location of individual coral colonies. Experimental evaluations conducted in controlled pool environments demonstrated the high accuracy of the proposed method, with an average accuracy of 90.7% in estimating coral coverage at different locations. Moreover, field trials conducted in Xidao Island of China verified the effectiveness and robustness of the CDRA algorithm under varying water quality and lighting conditions. The findings of this study represent a significant step towards the development of reliable and automated techniques for coral reef detection, contributing to the advancement of coral reef conservation efforts.

Changes in the community structure of stony corals in the southern Mexican Caribbean

The Mexican Caribbean coral reef ecosystem has endured the effects of global and regional stressors and, recently, the massive arrivals of the free-living, floating brown algae Sargassum spp. This study aimed to evaluate spatiotemporal changes in the stony coral community structure in the southern Mexican Caribbean by a temporal comparison of live coral cover and colony density using a data set collected in 2008–2009 and a recent survey in 2021 within a Protected Natural Area. A multivariate analysis approach was used to reveal spatiotemporal changes in coral cover and colony densities. Coral cover ranged from 6.9 to 8.9% in 2008–2009 to 6.5% in 2021, the lowest values recorded for the area. Coral colony density ranged from 0.68 to 0.78 colonies m−1 in 2008–2009 to 0.68 colonies m−1 in 2021. The present results appear to represent subtle changes during the last decade.

Stony coral tissue loss disease intervention with amoxicillin leads to a reversal of disease-modulated gene expression pathways.

Stony coral tissue loss disease (SCTLD) remains an unprecedented disease outbreak due to its high mortality rate and rapid spread throughout Florida's Coral Reef and wider Caribbean. A collaborative effort is underway to evaluate strategies that mitigate the spread of SCTLD across coral colonies and reefs, including restoration of disease-resistant genotypes, genetic rescue, and disease intervention with therapeutics. We conducted an in-situ experiment in Southeast Florida to assess molecular responses among SCTLD-affected Montastraea cavernosa pre- and post-application of the most widely used intervention method, CoreRx Base 2B with amoxicillin. Through Tag-Seq gene expression profiling of apparently healthy, diseased, and treated corals, we identified modulation of metabolomic and immune gene pathways following antibiotic treatment. In a complementary ex-situ disease challenge experiment, we exposed nursery-cultured M. cavernosa and Orbicella faveolata fragments to SCTLD-affected donor corals to compare transcriptomic profiles among clonal individuals from unexposed controls, those exposed and displaying disease signs, and corals exposed and not displaying disease signs. Suppression of metabolic functional groups and activation of stress gene pathways as a result of SCTLD exposure were apparent in both species. Amoxicillin treatment led to a 'reversal' of the majority of gene pathways implicated in disease response, suggesting potential recovery of corals following antibiotic application. In addition to increasing our understanding of molecular responses to SCTLD, we provide resource managers with transcriptomic evidence that disease intervention with antibiotics appears to be successful and may help to modulate coral immune responses to SCTLD. These results contribute to feasibility assessments of intervention efforts following disease outbreaks and improved predictions of coral reef health across the wider Caribbean.

Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD).

Effective treatment and prevention of any disease necessitates knowledge of the causative agent, yet the causative agents of most coral diseases remain unknown, in part due to the difficulty of distinguishing the pathogenic microbe(s) among the complex microbial backdrop of coral hosts. Stony coral tissue loss disease (SCTLD) is a particularly destructive disease of unknown etiology, capable of transmitting through the water column and killing entire colonies within a matter of weeks. Here we used a previously described method to (i) isolate diseased and apparently healthy coral colonies within individual mesocosms containing filtered seawater with low microbial background levels; (ii) incubate for several days to enrich the water with coral-shed microbes; (iii) use tangential-flow filtration to concentrate the microbial community in the mesocosm water; and then (iv) filter the resulting concentrate through a sequential series of different pore-sized filters. To investigate the size class of microorganism(s) associated with SCTLD transmission, we used 0.8 µm pore size filters to capture microeukaryotes and expelled zooxanthellae, 0.22 µm pore size filters to capture bacteria and large viruses, and 0.025 µm pore size filters to capture smaller viruses. In an attempt to further refine which size fraction(s) contained the transmissible element of SCTLD, we then applied these filters to healthy "receiver" coral fragments and monitored them for the onset of SCTLD signs over three separate experimental runs. However, several factors outside of our control confounded the transmission results, rendering them inconclusive. As the bulk of prior studies of SCTLD in coral tissues have primarily investigated the associated bacterial community, we chose to characterize the prokaryotic community associated with all mesocosm 0.22 µm pore size filters using Illumina sequencing of the V4 region of the 16S rRNA gene. We identified overlaps with prior SCTLD studies, including the presence of numerous previously identified SCTLD bioindicators within our mesocosms. The identification in our mesocosms of specific bacterial amplicon sequence variants that also appear across prior studies spanning different collection years, geographic regions, source material, and coral species, suggests that bacteria may play some role in the disease.

The comparative effects of chronic microplastic and sediment deposition on the scleractinian coral Merulina ampliata

Despite increasing research into the effects of microplastics on corals, no study to date has compared this relatively novel pollutant with a well-established stressor such as downwelling sediments. Here, Merulina ampliata coral fragments were exposed to polyethylene terephthalate (PET) and calcium carbonate particles (200–300 μm) at two deposition levels, high (115.20 ± 5.83 mg cm−2 d−1, mean ± SE) and low (22.87 ± 1.90 mg cm−2 d−1) in specially-designed Flow-Through Resuspension (FloTR) chambers. After 28 d, there were no significant differences between fragments exposed to sediments and microplastics for coral skeletal growth, Symbiodiniaceae density, and areal or cellular chlorophyll a concentrations. There were also no significant differences between levels of treatments, or with the control fragments. More PET microplastic particles were incorporated into the coral skeletons of fragments exposed to microplastics compared to those exposed to sediment and the control fragments, but there was no difference between fragments exposed to high and low microplastic levels. Together, the results show that M. ampliata appears to be able to cope with both microplastic and sediment stress, and suggests that microplastics do not represent a more serious threat than downwelling sediments at the levels tested.

Symbiotic communities associated with nursery-reared and natural corals: are they similar?

Coral aquaculture techniques have been developed for many species based on a well-documented understanding of the factors that affect coral growth and fitness. However, there is limited knowledge about the composition and structure of ectosymbiotic communities associated with cultured coral colonies. To address this gap, we conducted a study ofPocillopora verrucosacolonies reared from fragments in fixed nurseries and exposed for 6 and 12 months, as well as natural colonies in the same location. Our analysis focused on the species composition, species richness, and density of obligate and facultative ectosymbionts associated with the colonies. Obtained results indicate that the community associated with nursery-reared colonies was highly diverse, comprising 25 ectosymbionts, including 9 obligate and 16 facultative species. The prevalence, species richness, and density of the communities associated with natural colonies were significantly higher than those associated with reared ones. We also observed differences in the communities associated with reared colonies of different exposures, but we were able to group them as stages of the same community based on the size and exposure of the colonies. The differences between communities associated with reared and natural colonies may be related to the microhabitat properties of the symbiotic communities, as the former were elevated above the substrate, while the latter were attached to it. The age of natural colonies was also estimated to be more than three times higher than that of reared colonies of the same size, contributing to the differences. Our study clearly demonstrates that coral nurseries not only serve as a means of propagating corals but also offer artificial habitats for the maintenance and conservation of associated fauna. These findings have important implications for the management and conservation of coral reefs.

La Niña‐related coral death triggers biodiversity loss of associated communities in the Galápagos

AbstractDuring a cold La Niña period (August 2007–January 2008) in the central Galápagos archipelago, 70% of Pocillopora branching corals were severely bleached across three long‐term monitoring sites, affording an opportunity to examine its impact on the persistence of these corals and their associated community of fish and mobile macroinvertebrates. Using a time series empirical approach, we tagged and tracked the fate of 96 coral heads and their associates. When surveyed in July 2008, recovered live and dead corals that were previously severely bleached supported similar levels of species richness (randomized observed and estimated Chao 1). By contrast, richness on the surviving live corals remained fairly stable, while Chao 1 estimated richness on dead corals underwent a nearly 50% increase between July and January 2009, thereafter declining to 50% of originally surveyed richness by February 2010. This nonlinear change in species richness was largely due to an influx and decline in opportunistic generalists including pencil urchin bioeroders, gastropod snails, and hermit crabs that colonized dead corals and fed on sessile invertebrates and algae that had initially recruited to dead and undefended coral substrate. Thus, dead corals retained high overall species richness until live corals had recovered, after which richness declined as dead corals eroded and disintegrated (July 2011). Live corals attracted a less speciose but stable assemblage of mutualistic xanthid crabs and fishes that increased in abundance over time with the recovery and growth of live coral tissue. Overall, coral status (live/dead), planar area and maximum branch length predicted the number of species associated with each colony. The delayed diversity loss of associated species following La Niña disturbance to a foundation species represents a local extinction debt of 32–49‐month duration. A better understanding of the scale of extinction debt in foundational marine ecosystems is needed to quantify the breadth of impacts of climate oscillations on biodiversity and ecosystem functioning.

Reconstructing past disturbance in coral communities using U-Th dating of dead coral skeletons

Abstract In the absence of detailed broad-scale studies, both spatially and temporally, the overall status (disturbed, recovering, or in decline) of many of the reefs that make up the Great Barrier Reef remains uncertain. Moreover, of the numerous and varied threats, their relative role in impacting individual reefs is generally unclear. Here, we adopt a novel approach to reliably reconstruct historical disturbance events at Rib and Davies Reefs, two mid-shelf reefs, using uranium-thorium (U-Th) dating of dead corals. Corrected 230Th ages obtained from dead Acropora spp. bracket time periods of lowest coral cover observed during independent ecological surveys in the late 1980s and 2000s at 1988.2 ± 1.3 and 2003.9 ± 6.2 CE, shortly after the arrival of crown-of-thorns starfish (CoTS) at Rib Reef in 1983–1985 and 2000–2002, respectively. At Davies Reef, 230Th ages dated to 1999.2 ± 1.2 CE when coral cover was halved as a result of Cyclone Tessi and an “unknown” disturbance in 2000–2001. Prior to modern surveys, there is remarkable overlap in 230Th ages between reefs, with repeated peaks in the age distribution having a return period of ~10–15 years, akin to the periodicity of modern CoTS outbreaks. Our findings suggest that U-Th dating of dead corals can provide a robust foundation for understanding disturbance history and show promise in contributing to the effective monitoring of coral communities by providing a reliable benchmark with which to assess recovery.

Underwater surveys reveal deep-sea corals in newly explored regions of the southwest Atlantic

Deep-sea coral distribution and composition are unknown in much of the global ocean, but repurposing ocean industry surveys can fill that gap. In Santos Basin, southeast Brazil, areas (241–963 m depth) were surveyed during seven Petrobras cruises, mapping bottom topography with multibeam sonar, then collecting video with remotely operated vehicles. Here, we defined deep-sea coral species distribution and richness, using these surveys, correlating them to physical oceanographic properties. Solenosmilia variabilis was the most prevalent colonial species in coral mounds. Overall, 67% of species were Octocorallia. Coral assemblage structure, abundance, and richness varied among sites both within and among depths, with higher density and richness in the northernmost Santos basin. Depth was the strongest predictor for scleractinian coral distribution, with depth ranges varying by species. Assemblage differences corresponded to changes in water mass. Desmophyllum pertusum was more abundant in South Atlantic Central Water and S. variabilis in Antarctic Intermediate Water influenced areas.

Sr/Ca-temperature calibration for the coral Montastraea cavernosa

The Sr/Ca ratio in coral skeletons is an important proxy for the reconstruction of sea surface temperatures (SST) of the environment and helps our knowledge about SST variability in the tropics. In the western tropical South Atlantic where the species Montastraea cavernosa is one of the most important reef-builders, only a few species have been evaluated as coral-based archives for environmental changes. Here we evaluate the Sr/Ca ratio recorded in the thecal wall and other skeletal structures of the species M. cavernosa and its implications for paleothermometry investigations. Sampling along the thecal wall generated a record of Sr/Ca ratios with well-defined annual cycles, but also with noisy intervals. A linear regression of the most recent 8 years of well-defined Sr/Ca ratios and SST results in the calibration equation Sr/Ca (mmol/mol) = −0.040 (±0.005) * SST (°C) + 10.15 (±0.15). This is the first calibration equation for this species. The skeletal elements of M. cavernosa were characterized by heterogeneous Sr/Ca ratios, with higher values for the columellae and septum, which could potentially generate a cold bias in reconstructed SST of up to 7.5 °C. The variability of Sr/Ca ratios in these structures overwhelms the annual seasonality of Sr/Ca ratios in the thecal wall, and the difference in Sr/Ca ratios observed from distinct skeletal structures may be related to biological or vital effects influencing the uptake of Sr into different coral structures. Furthermore, improving sampling techniques for M. cavernosa should be considered in future studies for the successful recovery of robust geochemical signals, eventually expanding the number of species used as natural archives in the western tropical South Atlantic.

Chasing Interannual Marine Paleovariability

AbstractSeveral modes of tropical sea‐surface temperature (SST) variability operate on year‐to‐year (interannual) timescales and profoundly shape seasonal precipitation patterns across adjacent landmasses. Substantial uncertainty remains in addressing how SST variability will become altered under sustained greenhouse warming. Paleoceanographic estimates of changes in variability under past climatic states have emerged as a powerful method to clarify the sensitivity of interannual variability to climate forcing. Several approaches have been developed to investigate interannual SST variability within and beyond the observational period, primarily using marine calcifiers that afford subannual‐resolution sampling plans. Amongst these approaches, geochemical variations in coral skeletons are particularly attractive for their near‐monthly, continuous sampling resolution, and capacity to focus on SST anomalies after removing an annual cycle calculated over many years (represented as geochemical oscillations). Here we briefly review the paleoceanographic pursuit of interannual variability. We additionally highlight recent research documented by Ong et al., (2022, https://doi.org/10.1029/2022PA004483) who demonstrate the utility of Sr/Ca variations in capturing SST variability using a difficult‐to‐sample meandroid coral species, Colpophyllia natans, which is widespread across the Caribbean region and can be used to generate records spanning multiple centuries.

Elemental Characterization of Indonesian Coral Skeleton Using Underwater Laser-Induced Breakdown Spectroscopy (LIBS), X-Ray Fluorescence Spectroscopy (XRF), and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES)

The identification and determination of elements from the Indonesian coral skeleton are required to characterize anthropogenic activities. To this end, various spectroscopy methods were used for elemental analysis including underwater laser-induced breakdown spectroscopy (LIBS), x-ray fluorescence spectroscopy (XRF), and inductively coupled plasma – optical emission spectroscopy (ICP-OES). The results show that the LIBS was able identify the major elements Ca, Mg, and Sr. Using ICP-OES, all elements including major, minor, and trace elements were quantitatively determined. The results show that coral skeletons from Karimunjawa contain the highest concentrations of trace elements, which may be due to expanded human activities.

Ploidy Variation and Its Implications for Reproduction and Population Dynamics in Two Sympatric Hawaiian Coral Species.

Standing genetic variation is a major driver of fitness and resilience and therefore of fundamental importance for threatened species such as stony corals. We analyzed RNA-seq data generated from 132 Montipora capitata and 119 Pocillopora acuta coral colonies collected from Kāne'ohe Bay, O'ahu, Hawai'i. Our goals were to determine the extent of colony genetic variation and to study reproductive strategies in these two sympatric species. Surprisingly, we found that 63% of the P. acuta colonies were triploid, with putative independent origins of the different triploid clades. These corals have spread primarily via asexual reproduction and are descended from a small number of genotypes, whose diploid ancestor invaded the bay. In contrast, all M. capitata colonies are diploid and outbreeding, with almost all colonies genetically distinct. Only two cases of asexual reproduction, likely via fragmentation, were identified in this species. We report two distinct strategies in sympatric coral species that inhabit the largest sheltered body of water in the main Hawaiian Islands. These data highlight divergence in reproductive behavior and genome biology, both of which contribute to coral resilience and persistence.

High densities, rapid infestation and high feeding rates of corallivore gastropods on corals in Bora-Bora Island, French Polynesia

The global decline of coral reefs has prompted research into understanding the impacts of anthropogenic stressors, as well as the interactions between corals and their natural predators, especially those that proliferate into outbreaks. However, studies on gastropod corallivores are lagging behind. The present study, conducted on Bora-Bora (French Polynesia), focused on two genera of corallivore muricid gastropods: Drupella spp. living on Acropora colonies and Coralliophila violacea on Porites rus colonies. An observational experiment of Drupella spp. and C. violacea showed strong spatial variation in densities across different habitats (fringing reef, channel, barrier reef) in Bora-Bora lagoon. The highest densities found were 21 ± 6 ind.m−3 of coral for Drupella spp. on the fringing reef, and 342 ± 51 ind.m−3 for C. violacea on the barrier reef. A manipulation experiment monitored the infestation dynamics of these gastropods. For both genera, colonization of vacant coral colonies was rapid during the first 14 days and then stabilized over the next 42 days. The manipulation experiment showed that the feeding rate on live and healthy coral tissue did not change significantly with Drupella spp. density (0.6 ± 0.2 cm 2/day/ind for 1 Drupella spp., and 0.5 ± 0.1 cm 2/day/ind for 5 Drupella spp.). Overall, our study highlights the high densities of two gastropod species, their rapid movement and infestation of vacant colonies and their role in the necrosis of corals during feeding. In view of the possible outbreaks involving high densities of such predatory gastropods in a major tourist destination in the Pacific Ocean, human resources could be put in place to manually remove these gastropods in a sustainable manner, but this would require a lot of effort in terms of manpower and time. Nature-based solutions such as the conservation of natural predators should be considered to prevent large-scale destructions by these gastropod aggregations.

The role and risks of selective adaptation in extreme coral habitats

The alarming rate of climate change demands new management strategies to protect coral reefs. Environments such as mangrove lagoons, characterized by extreme variations in multiple abiotic factors, are viewed as potential sources of stress-tolerant corals for strategies such as assisted evolution and coral propagation. However, biological trade-offs for adaptation to such extremes are poorly known. Here, we investigate the reef-building coral Porites lutea thriving in both mangrove and reef sites and show that stress-tolerance comes with compromises in genetic and energetic mechanisms and skeletal characteristics. We observe reduced genetic diversity and gene expression variability in mangrove corals, a disadvantage under future harsher selective pressure. We find reduced density, thickness and higher porosity in coral skeletons from mangroves, symptoms of metabolic energy redirection to stress response functions. These findings demonstrate the need for caution when utilizing stress-tolerant corals in human interventions, as current survival in extremes may compromise future competitive fitness.

The cold-water coral province of the eastern Ligurian Sea (NW Mediterranean Sea): historical and novel evidences

Several bathyal cold-water coral provinces, characterized by a lush growth of habitat-forming scleractinians, have been recognized in the Mediterranean Sea. However, the search for this biogenic habitat only marginally targeted the Italian coast of the Ligurian Sea (NW Mediterranean basin) despite historical and a few recent local studies in the region reporting the presence of corals. This study used bathymetry maps, side-scan sonar profiles, historical charts, and trawling routes to identify sites that could potentially host coral habitats in the eastern sector of the Ligurian Sea. Remotely operated vehicle video footage from various projects (2015-2021, 20 dives) was then used to characterize four sub-areas (Genoa Plateau, Portofino, Deiva Marina, and Monterosso) where corals were detected between 450 m and 750 m depth. Radiocarbon dating was used to trace back the geological history of the coral structures. A small coral mound, impacted by trawling activities, was found on the Genoa Plateau, while four massive coral structures were found in the other sub-areas, mainly located in a morphologically complex and highly energetic canyon region. High levels of megafaunal biodiversity, including rarely reported alcyonaceans as Placogorgia coronata, were observed together with moderate fishing impact. Overall, the identified coral areas potentially account for 9 km2 of both subfossil mounds (as old as 13300 years BP), dominated by Desmophyllum pertusum, and living reefs, dominated by Madrepora oculata, the latter representing up to 23% of the substrate coverage. The few living colonies of D. pertusum in the area represent the first documented records for the Ligurian Sea. These data support the presence of a distinct eastern Ligurian cold-water coral province.

Morphological and molecular analysis of the tropical hermit crab Calcinus vachoni (Decapoda, Diogenidae) and its potential association with colonial anemone.

Calcinus is the colorful hermit crab genus belonging to the family Diogenidae and is often found in coral reefs of the tropical Indo-West Pacific region, including southern Honshu, Japan, which is the northern limit of their occurrence. In the present study, we found C. vachoni for the first time in the intertidal zone of Jeju Island-the southernmost island of South Korea. We examined their morphology and provided a diagnosis of their morphological details with illustrations. In addition, the Korean Calcinus population was genetically characterized using mtDNA cox1 sequences and by placing them into three previously reported regional haplogroups. The phylogenetic tree from maximum likelihood analysis revealed that Korean C. vachoni is assigned to the C. vachoni haplogroups exclusively, one of the three well-supported mitochondrial haplogroups with distinct geographic ranges (i.e., C. vachoni, C. aff. vachoni Cooks, and C. aff. vachoni Mascarenes). This result provides new information on the species distribution of C.vachoni, extending their geographic range further north into the southern coast of Korea. In this study, we also first report the potential association of C. vachoni with their co-occurring colonial anemone species Palythoa aff. mutuki and dead coral head of Pocillopora species based on our on-site observation and a public coral collection database of Calcinus species. However, their ecological association with co-occurring coral species is putatively assumed for now and therefore has to be validated by compelling evidence from further field observation and experimental studies (i.e., whether the presence/absence of colonial anemones affects the behavior and survival of the hermit crabs).