Coral Research Articles

Additive manufacturing of NiTi lightweight porous structures bio-mimicking coral skeleton with enhanced mechanical properties and shape memory functions

Additive manufacturing of NiTi lightweight porous structures bio-mimicking coral skeleton with enhanced mechanical properties and shape memory functions

Variability of the surface boundary layer of reef-building coral species

The coral-seawater interface is an important, highly dynamic microenvironment for reef-building corals. Also known as the concentration boundary layer (CBL), it is a thin layer of seawater bordering the coral surface that dictates the biochemical exchange between the coral colony and bulk seawater. The CBL is thus a key feature that modulates coral metabolism. However, CBL variation among small-polyped coral species remains largely unknown. Therefore, we recorded over 100 profiles of dissolved O2 concentration using microsensors to characterize CBL traits (thickness, surface O2 concentration, and flux) of three small-polyped branching coral species, Acropora cytherea, Pocillopora verrucosa, and Porites cylindrica. Measurements were conducted during light and darkness combined with low or moderate water flow (2 and 6 cm s−1). We found that CBL traits differed among species. CBL thickness was lowest in A. cytherea, while P. verrucosa showed the largest depletion of surface O2 in dark and highest dark flux. In addition, we found that O2 concentration gradients in the CBL occurred with three main profile shapes: diffusive, S-shaped, and complex. While diffusive profiles were the most common profile type, S-shaped and complex profiles were more frequent in P. verrucosa and P. cylindrica, respectively, and prevailed under low flow. Furthermore, profile types differed in CBL thickness and flux. Finally, low flow thickened CBLs, enhanced changes in surface O2 concentration, and reduced flux, compared to moderate flow. Overall, our findings reveal CBL variability among small-polyped branching corals and help understand CBL dynamics in response to changes in light and water flow conditions.

Coral spawning patterns on the Luhuitou fringing reef in Hainan Island of the northern South China Sea

Observation of coral spawning times is valuable to detect spawning patterns and identify the potential mechanisms behind coral reproduction. Although large amount of data on global coral spawning records have become available over the past decades, information on coral spawning in the northern South China Sea remains scarce. In this study, we investigated the spawning patterns of scleractinian corals in Luhuitou fringing reef, Hainan Island, China, from 2009 to 2021 in relation to lunar cycles (month and day). The spawning times of 22 coral species from five genera (Acropora, Montipora, Platygyra, Dipsastraea, and Galaxea) within three families (Acroporidae, Merulinidae, and Euphylliidae) were recorded, with spawning occurring from lunar February to May 2009–2021. Recorded spawning events started at a period of increasing seawater temperature. Acropora, the most documented genus, spawned between lunar February and April, but primarily in lunar March. Importantly, the spawning time of Acropora was delayed for one lunar month in 2016 most likely due to a rapid decrease in monthly mean seawater temperature in lunar February. Spawning lunar days before, on or after full moon of corals in the Luhuitou Reef, including those of the Acropora species, were highly variable between years even for the same species. No predictable pattern of spawning times can thus be detected. Nonetheless, our results fill up an information gap on coral spawning patterns in the northern South China Sea that may be useful for further understanding of the reproductive biology of scleractinian corals throughout the Indo–West Pacific.

Microplastic pollution status in the coral reef ecosystems on the Southern and Western coasts of Sri Lanka during the Southwest monsoon

We investigated the microplastics (MP) abundance, characteristics and pollution severity in the six coral reef ecosystems along the southern and western coasts of Sri Lanka during the southwest monsoon. The small blue-coloured fibres dominated within coral reefs and reef environments. The average coral, surface water and surface sediment MP concentration were 806.64 ± 93.72 particles kg−1ww, 23.42 ± 5.01 particles m−3, and 54.11 ± 8.04 particles kg−1dw, respectively. Coral MP concentration was significantly correlated with surface water (r = 0.65) and surface sediments (r = 0.59) indicating that the reef environment was the source of coral MP enrichment. Coral MP concentration was spatially different (p < 0.05) which may likely be due to the changes in winds, waves, coral biology and plastic sources. MP Pollution Load Index indicated that low MP contamination hazard. Since the presence of MP often deteriorates coral's health, further investigations are necessary to understand the MP's effects on the health of coral reef ecosystems.

Ultrastructural evaluation of the oocytes and spermatozoa of the scleractinian coral Mussismilia harttii

Global coverage of living coral has declined by half since 1950s. Reef-building species have been severely impacted in this climate crisis scenario, compromising the future of coral reefs. Despite their importance, there is a lack of knowledge regarding the reproductive biology of scleractinian corals. In the present study, we evaluated through electron microscopy approaches, the gametes of the endemic Southwestern Atlantic coral Mussismilia harttii. We observed spherical oocytes with microvilli throughout the outer membrane. Fine granular material dispersed in cytoplasm, lipid granules, numerous yolk bodies, and mitochondria were identified in the oocytes. In addition, small Symbiodinium-like cells were observed, suggesting a vertical transmission from parental coral to oocytes. The spherical-head sperm presents a 9.3 ± 2.1 μm flagellum. The nucleus is located centrally in the head, and the centrioles are positioned between the nuclear base and the flagellar insertion, which is connected to the axoneme. This axoneme has a microtubular arrangement (9+2). Vesicles, underlining the inner plasma membrane, presented the same electron-dense pattern as the Golgi complex, and mitochondria positioned surrounding the axoneme. The vesicles present in the sperm may have a role as an acrosome since the oocytes do not develop any cell specialization for fertilization.

Microbiome heterogeneity in tissues of the coral, Fimbriaphyllia (Euphyllia) ancora.

Coral microbiomes differ in the mucus, soft tissue and skeleton of a coral colony, but whether variations exist in different tissues of a single polyp is unknown. In the stony coral, Fimbriaphyllia ancora, we identified 8,994 amplicon sequencing variants (ASVs) in functionally differentiated polyp tissues, i.e., tentacles, body wall, mouth and pharynx, mesenterial filaments, and gonads (testes and ovaries), with a large proportion of ASVs specific to individual tissues. However, shared ASVs comprised the majority of microbiomes from all tissues in terms of relative abundance. No tissue-specific ASVs were found, except in testes, for which there were only two samples. At the generic level, Endozoicomonas was significantly less abundant in the body wall, where calicoblastic cells reside. On the other hand, several bacterial taxa presented significantly higher abundances in the mouth. Interestingly, although without statistical confirmation, gonadal tissues showed lower ASV richness and relatively high abundances of Endozoicomonas (in ovaries) and Pseudomonas (in testes). These findings provide evidence for microbiome heterogeneity between tissues within coral polyps, suggesting a promising field for future studies of functional interactions between corals and their bacterial symbionts.

Synthetic and practical reconstructions of SST and seawater pH using the novel multiproxy SMITE method.

Geochemical proxies of sea surface temperature (SST) and seawater pH (pHsw) in scleractinian coral skeletons are valuable tools for reconstructing tropical climate variability. However, most coral skeletal SST and pHsw proxies are univariate methods that are limited in their capacity to circumvent non-climate-related variability. Here we present a novel multivariate method for reconstructing SST and pHsw from the geochemistry of coral skeletons. Our Scleractinian Multivariate Isotope and Trace Element (SMITE) method optimizes reconstruction skill by leveraging the covariance across an array of coral elemental and isotopic data with SST and pHsw. First, using a synthetic proxy experiment, we find that SMITE SST reconstruction statistics (correlation, accuracy, and precision) are insensitive to noise and variable calibration period lengths relative to Sr/Ca. While SMITE pHsw reconstruction statistics remain relative to δ11B throughout the same synthetic experiment, the magnitude of the long-term trend in pHsw is progressively lost under conditions of moderate-to-high analytical uncertainty. Next, we apply the SMITE method to an array of seven coral-based geochemical variables (B/Ca, δ11B, Li/Ca, Mg/Ca, Sr/Ca, U/Ca & Li/Mg) measured from two Bermudan Porites astreoides corals. Despite a <3.5 year calibration period, SMITE SST and pHsw estimates exhibit significantly better accuracy, precision, and correlation with their respective climate targets than the best single- and dual-proxy estimators. Furthermore, SMITE model parameters are highly reproducible between the two coral cores, indicating great potential for fossil applications (when preservation is high). The results shown here indicate that the SMITE method can outperform the most common coral-based SST and pHsw reconstructions methods to date, particularly in datasets with a large variety of geochemical variables. We therefore provide a list of recommendations and procedures for users to begin implementing the SMITE method as well as an open-source software package to facilitate dissemination of the SMITE method.

Effects of flows on transparent exopolymer particles released from branching Acropora coral colonies

Transparent exopolymer particles (TEP), a major component of coral mucus, are responsible for particle aggregation. These particles contribute substantially to the carbon cycle in coral reefs, and serve as an energy source for bacteria and other microorganisms. Water flows and induced turbulent mixing control material exchange between the coral canopy and the surrounding water, which is critical for coral health. However, how these factors affect TEP release by coral colonies has yet to be evaluated. Using a recirculating flume, we assessed TEP release by branching Acropora coral colonies and associated bacterial growth in the water column under different unidirectional flows. Changes in TEP and bacterial concentrations after 24-h incubation were quantified for flow speeds of 0, 5, 10, and 30 cm/s. Particle image velocimetry (PIV) measurements provided an estimate of turbulent mixing efficiency above the coral canopy. TEP and bacterial concentrations in the water column increased after 24 h of incubation. The increase in TEP and bacterial concentrations were 6.2–9.3 times and 3.4–5.1 times higher in the absence of flows, respectively, than mean values under water flows. Although mixing efficiency increased linearly with mean flow speeds, TEP release and bacterial growth differed only marginally at flows ranging from 5–30 cm/s. Detailed flow measurements combined with evaluation of TEP release suggest that the complex geometry of corals facilitates efficient material exchange at a range of flow speeds, and highlight the importance of considering these factors when estimating coral reef biogeochemistry.

Ocean acidification in the tropical Indian Ocean over the past 37 years: Insights from [formula omitted]11B and B/Ca records in a Maldives coral

Boron isotopes (δ11B) in coral skeletons of Porites have been widely applied to reconstruct past seawater pH (pHSW) on decadal to centennial timescales. However, due to biological regulation within corals, an additional transfer function is required to estimate ambient seawater chemistry during the skeleton growth under the calcification site fluid pH. Temperature may also interfere with coral calcification fluid pH (pHCF) due to changes in kinetics of coral aragonite precipitation, or buffering capacity in coral calcification fluid. To decipher how coral Porites adjusts pHCF in response to pHSW from complex environmental controls, long-term records from sites with least fluctuations in environmental conditions other than pHSW are essential. Here we present a 37-year record of coral δ11B and B/Ca ratios derived from a coral core collected from southern Maldives, the tropical Indian Ocean. Our results show no clear seasonality in the coral δ11B and B/Ca ratios between monsoons, but a long-term decline in coral pHCF is evident across the entire record. When applying different existing transfer functions, we also observe discrepancies among the calculated pHCF values, model results and short-term instrumental data. Calculated calcification fluid dissolved inorganic carbon concentration ([DIC]CF) values are relatively low compared to literature, suggesting that coral calcification fluid carbonate chemistry may be under different levels of control, even within the same coral taxa. Thus, coral records from a wider geographic range are required to better quantify coral response to ocean acidification, and our results can serve as a baseline for future comparisons.

Reef location and season, but not recruitment substrate contour and composition, affect coral recruitment patterns

Most studies have quantified coral recruitment using recruitment tiles temporarily deployed on reefs. However, the wide range of tile types used in different studies potentially influences recruitment patterns thereby hindering accurate comparisons among reef areas. We examined the effect of different tile types with different surface structure and composition on spatial (reef locations) and temporal (season) patterns of coral recruitment in the northwestern Philippines. Dead coral skeleton, terracotta, and fibre-cement tiles were deployed and retrieved quarterly over a 15-month period. In contrast to previous studies, patterns of standardized density and composition of recruits were consistent among tile types. Recruits varied spatially and were highest in Caniogan reef, followed by Cory reef and Lucero reef, suggesting that coral recruitment in the Bolinao-Anda Reef Complex (BARC) is influenced by reef location and existing coral cover. Highest recruitment was also found during the peak coral spawning season. The results of this study contrast with some previous reports which indicate that coral recruitment patterns are strongly influenced by recruitment substrate types. Our study suggests that once sufficient biological conditioning of the tile surfaces has occurred, the microbial and algal community present on the different tile surfaces are similarly conducive to larval settlement of some coral taxa.

Three-dimensional reconstruction of high latitude bamboo coral via X-ray microfocus Computed Tomography

The skeletons of long-lived bamboo coral (Family Keratoisididae) are promising archives for deep-water palaeoceanographic reconstructions as they can record environmental variation at sub-decadal resolution in locations where in-situ measurements lack temporal coverage. Yet, detailed three dimensional (3D) characterisations of bamboo coral skeletal architecture are not routinely available and non-destructive investigations into microscale variations in calcification are rare. Here, we provide high-resolution micro-focus computed tomography (µCT) data of skeletal density for two species of bamboo coral (Acanella arbuscula: 5 specimens, voxel size, 15 µm (central branch scans) and 50 µm (complete structure scan); Keratoisis sp.: 4 specimens, voxel size, 15 µm) collected from the Labrador Sea and Baffin Bay deep-water basins, Eastern Canadian Arctic. These data provide reference models useful for developing methods to assess structural integrity and other fine-scale complexities in many biological, geological, and industrial systems. This will be of wider value to those investigating structural composition, arrangement and/or composition of complex architecture within the fields and subdisciplines of biology, ecology, medicine, environmental geology, and structural engineering.

Interaction between active tectonics, bottom-current processes and coral mounds: A unique example in the NW Moroccan Margin, southern Gulf of Cadiz

The NW Moroccan Margin has a complex geological evolution, being located close to the transition zone between the Azores – Gibraltar Fracture Zone and the western front of the Betic–Rif collisional orogen. The interaction between tectonic, halokinetic and fluid flow processes with bottom-current activity shapes the seafloor and influences the distribution of seafloor biological communities (such as the cold-water coral mounds) and deep-water sedimentation. The aims of this work are to study the interaction of the paleo-oceanographic and morpho-tectonic processes that generated the various seafloor features of the NW Moroccan Margin. To achieve this, high-resolution multibeam bathymetry and parasound data acquired in the “ALBOCA II” cruise have been used, complemented by high-resolution 2D seismic reflection data and the EMODnet bathymetric compilation.Several morphological features were identified in the margin, which are related to different processes of sedimentary (contourites and sediment waves), structural (faults and diapirs), gravitational (slide scars, mass transport deposits), fluid migration (mud volcanoes and pockmarks) and biogenic (exposed and buried coral mounds) nature. The structural features (e.g., strike-slip faults) have a major control on the seafloor morphology and, consequently, on the development and evolution of the sedimentary systems in the study area.The evolution of the NW Moroccan Margin during the late Quaternary has been controlled by climatic variations and tectonic activity. The action of these factors has been dominant in distinct parts of the study area where: i) contourite terraces developed when climatic and oceanographic changes were the prevalent factor, ii) mounded and confined contourite drifts and local mass transport deposits formed when the major control was tectonic activity.

Effects of water flow and ocean acidification on oxygen and pH gradients in coral boundary layer

Reef-building corals live in highly hydrodynamic environments, where water flow largely controls the complex chemical microenvironments surrounding them—the concentration boundary layer (CBL). The CBL may be key to alleviate ocean acidification (OA) effects on coral colonies by partially isolating them. However, OA effects on coral CBL remain poorly understood, particularly under different flow velocities. Here, we investigated these effects on the reef-building corals Acropora cytherea, Pocillopora verrucosa, and Porites cylindrica. We preconditioned corals to a control (pH 8.0) and OA (pH 7.8) treatment for four months and tested how low flow (2 cm s−1) and moderate flow (6 cm s−1) affected O2 and H+ CBL traits (thickness, surface concentrations, and flux) inside a unidirectional-flow chamber. We found that CBL traits differed between species and flow velocities. Under OA, traits remained generally stable across flows, except surface pH. In all species, the H+ CBL was thin and led to lower surface pH. Still, low flow thickened H+ CBLs and increased light elevation of surface pH. In general, our findings reveal a weak to null OA modulation of the CBL. Moreover, the OA-buffering capacity by the H+ CBL may be limited in coral species, though low flow could enhance CBL sheltering.

Rubble persistence under ocean acidification threatened by accelerated bioerosion and lower-density coral skeletons.

As the balance between erosional and constructive processes on coral reefs tilts in favor of framework loss under human-induced local and global change, many reef habitats worldwide degrade and flatten. The resultant generation of coral rubble and the beds they form can have lasting effects on reef communities and structural complexity, threatening the continuity of reef ecological functions and the services they provide. To comprehensively capture changing framework processes and predict their evolution in the context of climate change, heavily colonized rubble fragments were exposed to ocean acidification (OA) conditions for 55 days. Controlled diurnal pH oscillations were incorporated in the treatments to account for the known impact of diel carbonate chemistry fluctuations on calcification and dissolution response to OA. Scenarios included contemporary pH (8.05 ± 0.025 diel fluctuation), elevated OA (7.90 ± 0.025), and high OA (7.70 ± 0.025). We used a multifaceted approach, combining chemical flux analyses, mass alteration measurements, and computed tomography scanning images to measure total and chemical bioerosion, as well as chemically driven secondary calcification. Rates of net carbonate loss measured in the contemporary conditions (1.36 kg m-2 year-1) were high compared to literature and increased in OA scenarios (elevated: 1.84 kg m-2 year-1 and high: 1.59 kg m-2 year-1). The acceleration of these rates was driven by enhanced chemical dissolution and reduced secondary calcification. Further analysis revealed that the extent of these changes was contingent on the density of the coral skeleton, in which the micro- and macroborer communities reside. Findings indicated that increased mechanical bioerosion rates occurred in rubble with lower skeletal density, which is of note considering that corals form lower-density skeletons under OA. These direct and indirect effects of OA on chemical and mechanical framework-altering processes will influence the permanence of this crucial habitat, carrying implications for biodiversity and reef ecosystem function.

B(OH)4− and CO32− do not compete for incorporation into aragonite in synthetic precipitations at pHtotal 8.20 and 8.41 but do compete at pHtotal 8.59

Coral skeletal B/Ca (effectively B/CO32–), in combination with boron isotopic composition (δ11B), has been used to reconstruct the dissolved inorganic carbon chemistry of coral calcification media and to explore the biomineralisation process and its response to ocean acidification. This approach assumes that B(OH)4−, the B species incorporated into aragonite, competes with dissolved inorganic carbon species for inclusion in the mineral lattice. In this study we precipitated aragonite from seawater in vitro under conditions that simulate the compositions of the calcification media used to build tropical coral skeletons. To deconvolve the effects of pH and [CO32–] on boron incorporation we conducted multiple experiments at constant [CO32–] but variable pH and at constant pH but variable [CO32–], both in the absence and presence of common coral skeletal amino acids. Large changes in solution [CO32–], from < 400 to >1000 µmol kg−1, or in precipitation rate, have no significant effect on aragonite B/Ca at pHtotal of 8.20 and 8.41. A significant inverse relationship is observed between solution [CO32–] and aragonite B/Ca at pHtotal = 8.59. Aragonite B/Ca is positively correlated with seawater pH across precipitations conducted at multiple pH but this relationship is driven by the effect of pH on the abundance of B(OH)4– in seawater. Glutamic acid and glycine enhance the incorporation of B in aragonite but aspartic acid has no measurable effect. Normalising aragonite B/Ca to solution [B(OH)4–] creates KDB(OH)4− which do not vary significantly between pH treatments. This implies that B(OH)4– and CO32– do not compete with each other for inclusion in the aragonite lattice at pHtotal 8.20 and 8.41. Only at high pH (8.59), when [B(OH)4–] is high, do we observe evidence to suggest that the 2 anions compete to be incorporated into the lattice. These high pH conditions represent the uppermost limits reliably measured in the calcification media of tropical corals cultured under present day conditions, suggesting that skeletal B/Ca may not reflect the calcification media dissolved inorganic carbon chemistry in all modern day corals.

Recovery potential of fish and coral populations following ecological disturbance

AbstractThe health of coral reef benthic and fish communities is implicitly connected, yet typically studied and managed separately. By developing a coupled reef population model that connects coral populations and reef fish biomass through the habitat complexity that corals build and fish live among, we aim to address this gap by holistically quantifying ecological feedbacks and responses to ecological stressors. We explored the impacts of fishing effort in conjunction with three types of ecological disturbances as they propagated through a coral reef ecosystem: (1) a disturbance that disproportionately affected small, bio‐energetically vulnerable colonies, (2) a disturbance that predominantly affected large, mechanically vulnerable colonies, and (3) a disturbance that affected colonies of all sizes randomly. We found that joint coral and fish population recovery was fastest and most complete under events affecting small colonies, followed by recovery from disturbances affecting random sizes, and lastly large‐colony disturbances. These results suggest that the retention versus loss of large coral colonies with high reproductive potential critically influenced population recovery. Low fishing levels maintained fish and coral populations and allowed for recovery after disturbances, whereas high fishing levels prevented recovery due to greater fish‐dependent coral mortality. Finally, we tested various formulations of the relationship between coral size and habitat complexity (i.e., exponential, linear, logarithmic) that constrain fish carrying capacity. All formulations led to similar population projections in most disturbance scenarios, but there were exceptions where the timing and trajectory of recovery differed, such as faster and greater recovery potential when complexity is logarithmic with respect to coral size. These findings suggest that fishing and habitat complexity mediate the recovery of coral reef populations, emphasizing the importance of describing linkages between coral size distribution and reef habitat structure. Furthermore, our results highlight the utility of the coupled‐model framework for understanding and managing the impact of disturbances at ecosystem scales.

Thử nghiệm phục hồi san hô trên các giá thể reefball tại khu vực Đầm Báy, vịnh Nha Trang

340 hard coral colonies have been planted on 34 reefball constructions. The results show that survival rate ranged from 64.7 to 79.4%, the average of 69.4%, the highest survival rate was for the species Pocillopora verrucosa 79.4% and the lowest is for the species Acropora formosa 64.7%. The average growth rate of Acropora nobilis is about 7.7 mm per month, and one of Acropora formosa is around 6.7 mm per month. For table corals Acropora latistella, the average growth rate is approximately 9.6 mm per month, and for Acropora hyacinthus, the average growth rate is 7.7 mm/month. For species of cauliflower coral Pocillopora verrucosa has an average growth rate around 7.9 mm per month. The number of fishes species have increased from 3 to 10 species and the number of individuals increased from 16 individuals per 100m2 to 453 individuals per 100m2, of which the largest increase was Dascyllus trimaculatus up to 170 individuals per 100m2. Coral larvae have attached and grown on all 34 reefball constructions after one year. The recruit number varied between 7 - 15 colonies per reefball. A total of 325 coral larvae have settled on 34 reefball constructions, an average of 9.6 larvae per 1 construction. The diameter of coral larvae which were growing on reefball varied betwen 4-55 mm. Coral larvae mainly gather on the vertical surface of the substrate. The majority of coral larvae are Pocillopora verrucosa species, accounting for 69.9% (227 colonies). This study shows that reefball constructions can be effectively used as optimal means for rehabilitating damaged coral reefs.

In situ lesion recovery of Scleractinian branching coral wild colonies from asexual coral propagation

As coral reefs worldwide continue to degrade due to natural and anthropogenic threats, coral restoration efforts have been rapidly growing in recent decades to maintain the ecological function of coral reefs. In Malaysia, asexual propagation by fragmenting branches from healthy Acropora wild colonies is becoming the norm, but other coral genera are rarely considered in restoration efforts despite the need to enhance diversity and reef resilience. Additionally, restoration efforts in Malaysia mainly focus on the recovery of coral fragments in nurseries while overlooking the wild coral colonies they were sourced from. Lesion recovery from physical damage should be investigated to maximise restoration success and to ensure the wild coral populations do not degrade due to fragmentation from coral propagation efforts. To address this, we investigated the recuperation capabilities of two branching coral species, Acropora muricata and Echinopora horrida, in a shallow reef (<8 m) of Pulau Rawa, Johor, Malaysia. Coral colonies were induced with physical damage, and lesion recovery was measured via lesion site closure, colour score and relative growth of the broken colonies (n=30) over 129 days. The lesion site of A. muricata closed significantly more at 99.94±0.04SE%, compared to E. horrida with 86.97±3.53SE%. When compared to E. horrida, A. muricata had achieved full colour recovery by day 27 and more than double the value of relative growth (9.7 %). The differences in lesion recovery capabilities between A. muricata and E. horrida are hypothesised to be attributed to their different life adaptive strategies where A. muricata is an r adaptive strategist and E. horrida is a K adaptive strategist. The findings imply that future coral restoration efforts should investigate the recovery capabilities of the wild coral colonies before starting coral propagation efforts to prevent partial mortality of wild colonies and maintain species diversity and ecological functions of the reef.

Coral Health and Benthic Composition of Reefs in an MPA and Open-access Site in Western Calatagan, Batangas

Monitoring hard coral health and diversity is essential in assessing their resilience to disturbances such as coral bleaching. This study gathered baseline data on the health and benthic composition of coral reefs in western Calatagan, Batangas, the Philippines. Coral health in two sites – Buntong Gasang and the Quilitisan Marine Protected Area (MPA) – was assessed using the CoralWatch citizen science Coral Health Chart method. The average color score of more than 200 coral colonies was compared between sites and coral growth forms (i.e. branching, boulder, and plate). In addition, benthic composition in both sites was determined using photoquadrats. Buntong Gasang Point and Quilitisan MPA had mean coral scores that are 4.4 (± 1.0 SD) and 3.6 (± 1.0 SD), respectively, thus indicating normal symbiont densities. The analysis also showed little differences in mean color scores among coral growth forms ranging from 3.8–4.3. Both sites had relatively high coral cover (mean = 52.3% ± 4.3% SD in Buntong Gasang, mean = 49.7% ± 8.7% SD in Quilitisan MPA) despite having different benthic compositions [PERMANOVA, F(1,8) = 8.87, p = 0.006]. Branching coral cover was about six times higher in Buntong Gasang Point (17%), whereas encrusting corals dominated at Quilitisan MPA (~ 39%). In addition, algal cover was higher in Quilitisan (45.0%) than in Buntong Gasang (35.5%). Although other studies show that branching corals are less resistant than massive or encrusting forms to coral bleaching, examining coral color suggests that reefs in both Buntong Gasang and Quilitisan MPA appeared to have comparably recovered from the mass bleaching events in 2020–2021. However, regular monitoring is needed to assess the responses of these reefs to future thermal stress. This study shows the potential of monitoring coral color in assessing coral reef health in the Philippines.

Measuring multi-year changes in the Symbiodiniaceae algae in Caribbean corals on coral-depleted reefs.

Monitoring coral cover can describe the ecology of reef degradation, but rarely can it reveal the proximal mechanisms of change, or achieve its full potential in informing conservation actions. Describing temporal variation in Symbiodiniaceae within corals can help address these limitations, but this is rarely a research priority. Here, we augmented an ecological time series of the coral reefs of St. John, US Virgin Islands, by describing the genetic complement of symbiotic algae in common corals. Seventy-five corals from nine species were marked and sampled in 2017. Of these colonies, 41% were sampled in 2018, and 72% in 2019; 28% could not be found and were assumed to have died. Symbiodiniaceae ITS2 sequencing identified 525 distinct sequences (comprising 42 ITS2 type profiles), and symbiont diversity differed among host species and individuals, but was in most cases preserved within hosts over 3 yrs that were marked by physical disturbances from major hurricanes (2017) and the regional onset of stony coral tissue loss disease (2019). While changes in symbiont communities were slight and stochastic over time within colonies, variation in the dominant symbionts among colonies was observed for all host species. Together, these results indicate that declining host abundances could lead to the loss of rare algal lineages that are found in a low proportion of few coral colonies left on many reefs, especially if coral declines are symbiont-specific. These findings highlight the importance of identifying Symbiodiniaceae as part of a time series of coral communities to support holistic conservation planning. Repeated sampling of tagged corals is unlikely to be viable for this purpose, because many Caribbean corals are dying before they can be sampled multiple times. Instead, random sampling of large numbers of corals may be more effective in capturing the diversity and temporal dynamics of Symbiodiniaceae metacommunities in reef corals.