Copticum Extract Research Articles

In vitro anti-mycobacterial property and phytochemical profiling of Senna auriculata and Trachyspermum copticum

Mycobacterium tuberculosis is a contagious disease that is the leading cause of death by a single causative organism. The present study aims to investigate and assess the anti-mycobacterial property of some traditionally important medicinal plants and analyze their phytochemical constitution. The extracts of some plants based on their medicinal importance were checked for their activity against M. smegmatis using anti-mycobacterial disc diffusion plate assay. Aqueous extracts of Senna auriculata, Trachyspermum copticum and Musa spp. were tested against M. smegmatis. Further Senna auriculata and Trachyspermum copticum extracts were taken to bioactivity guided sequential extraction. On screening, ethyl acetate extract of S. auriculata, T. copticum and methanol extract of S. auriculata showed considerable inhibition with an average zone of inhibition of about 2 cm dia against M. smegmatis. Further phytochemical analysis was done to narrow down the class of compounds that might be responsible for the inhibitory activity. The tests revealed the presence of some important group of natural compounds like alkaloids, anthocyanins, tannins, β- cyanins, coumarins and terpenoid. GC-MS analysis of the samples showed some biologically important compounds with potent anti-tubercular activity.

Evaluation of in vitro antibacterial activity of Carum copticum extract against drug resistant Staphylococcus aureus from clinical and food sources

Staphylococcus aureus is commensal and human pathogen both. The bacterium is one of the most common causes of food borne infections also in all over the world. C. copticum (Ajwain) is an aromatic spice used as a medicinal plant from ancient time for treating different human diseases including infectious diseases. The highest anti-staphylococcal activity in terms of inhibition zone was recorded 18.5 mm whereas 14.5 mm as lowest. The MIC range of ethanolic extract was evaluated amid 1.56 mg/ml to 3.12 mg/ml. The C. copticum (seed) extract might be useful as anti-staphylococcal agent as well as natural food preservative.

Ajwain (Trachyspermum copticum) extract in broiler diets: effect on growth performance, carcass components, plasma constituents, immunity and cecum microflora

Hypothesis is that dietary ajwain (Trachyspermum copticum L.), a phytogenic feed additive, could improve the performance of broiler chickens. On a total of 200 Ross 308 male broilers, during the 42-d growing period (starter: day 1–14; grower: day 15–28 and finisher: day 29–42), varying levels of ajwain alcoholic extract (0, 150, 350 and 450 ppm) were added to the drinking water. A control treatment containing the Virginiamycin antibiotic was also studied. Within each treatment and period, the performances were evaluated and the Economic index calculated. On day 42, two birds per pen were killed and the carcase and organ weights determined. On day 42, on two birds per pen, blood samples were collected on the farm to study the serum parameters. On birds, vaccinated against sheep red blood cells at 14 and 35 days, blood samples were collected at 21 and 42 days of age to determine total Ig, IgG and IgM antibodies and, at day 42, the spleen and bursa of Fabricius were weighed. The microflora on the caecum content was counted. Ajwain extract significantly (p < .05) influenced productive performances, blood parameters, immune parameters and gut microbiota, indicating its possible role as a substitute for antibiotics in a sustainable poultry industry.

Effective inhibition and eradication of pathogenic biofilms by titanium dioxide nanoparticles synthesized using Carum copticum extract.

Most bacteria exist in nature in the form of biofilms. One of the key survival strategies by bacteria to withstand chemical and physical stresses is by forming biofilms on biotic and abiotic surfaces. A different set of genes are expressed in biofilms compared to the planktonic mode of bacterial growth. According to data from the National Institutes of Health (NIH) and Centers for Disease Control and Prevention (CDC), nearly 80 percent of all human infections are encouraged by biofilms and roughly 65 percent of all hospital-acquired infections are associated with biofilms. Hence, considering the role of biofilms in clinical settings, there is an urgent need for the discovery/development of novel antibiofilm agents. In this study, we have tested the effect of freshly prepared titanium dioxide nanoparticles (TiO2-NPs) synthesized using Carum copticum extract on biofilms, both against Gram +ve and Gram −ve bacteria. Being environment friendly in nature, the green route of nanoparticle synthesis is believed to be advantageous over chemical synthesis of metal nanoparticles. The synthesized nanoparticles were found to be predominantly spherical or spheroidal in shape with an average size of 12.01 ± 5.58 nm. As evident from data, more than 70% inhibition of biofilms of test bacteria was achieved in the presence of TiO2-NPs. Electron microscopic analysis revealed that the adherence and colonization of bacteria on the glass surface were remarkably reduced by the treatment of TiO2-NPs. The EPS secretion of E. coli ATCC 25922 and P. aeruginosa PAO1 were inhibited by 62.08 and 74.94%, respectively. The EPS secretion of S. aureus MTCC 3160 was least inhibited (<55%) compared to other test bacteria. Moreover, TiO2-NPs successfully eradicated the preformed biofilms of E. coli ATCC 25922, P. aeruginosa PAO1, and S. aureus MTCC 3160 by 60.09, 64.14, and 48.30%, respectively. The findings demonstrate the efficacy of green synthesized titanium dioxide nanoparticles in inhibiting and eradicating the biofilms of bacterial pathogens and they may be further exploited for the development of a new alternative antibiofilm agent.

Study the antibacterial and antibiofilm activity of Carum copticum against antibiotic-resistant bacteria in planktonic and biofilm forms

ObjectivesMicrobial biofilms has attracted interest in the recent years because they has become the most important cause of nosocomial infections. This study was aimed to examine the antibacterial activities of Carum copticum extracts on the development of microbial biofilms and planktonic form of six pathogenic bacteria. MethodsAntimicrobial activity of the crude extracts against the planktonic form of six pathogenic bacteria: Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii and Klebsiella pneumonia was evaluated by using the disc diffusion method. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values was determined by macro-broth dilution technique. Anti-biofilm effects were assessed by microtiter plate method. The chemical composition of the herbal extract was identified by GC-MS. ResultsAccording to disc diffusion test (MIC and MBC) the ability of C. copticum extracts for inhibition of bacteria in planktonic form was confirmed. The best inhibitory effect of this plant on S. aureus and low inhibitory effect on A. baumannii in planktonic forms were observed. These extracts were efficient to inhibit biofilm structures and concentration of each extract has direct relation with inhibitory effect. The maximum and minimum inhibitory effects of C. copticum methanolic extract on biofilm formation were observed on A. baumannii (98%) and K. pneumoniae (19%) respectively. ConclusionThe GC-MS analysis revealed that five active compounds were present in the extract of this plant. Data obtained, suggested that the C. copticum extracts applied as antimicrobial agents against these pathogens particularly in biofilm making.

Bioactive extracts of Carum copticum L. enhances efficacy of ciprofloxacin against MDR enteric bacteria.

The widespread occurrence of extended spectrum β-lactamases (ESβLs) producing enteric bacteria and their co-resistance with flouroquinolones has impaired the current antimicrobial therapy. This has prompted the search for new alternatives through synergistic approaches with herbal extracts. In this study Carum copticum (seeds) was extracted first in methanol and then subsequently extracted in different organic solvents. MIC of plant extracts, ciprofloxacin and thymol was determined by broth micro-dilution method using TTC. Synergism between plant extracts and ciprofloxacin was assayed by the checkerboard method. Chemical constituents of active extracts were analyzed by GC-MS. Methanolic, hexane and ether extract of Carum copticum exhibited significant antibacterial activity with MIC values ranged from 0.25 mg/ml to 2.0 mg/ml. Synergy analysis between Carum copticum extracts and ciprofloxacin combinations revealed FIC index in the range of 0.093–0.25. About 81% ciprofloxacin resistant ESβL producing enteric bacteria were re-sensitized in the presence of 15.6–250 μg/ml of methanolic extract of Carum copticum. Moreover, ciprofloxacin showed 8 to 64 folds reduction in MIC in presence of 250 and 500 μg/ml of hexane extract. Whereas, 4–32 folds reduction in MIC of ciprofloxacin was achieved in the presence of 31.25 and 62.5 μg/ml of ether extract, indicating synergistic enhancement of drug activity. The chemical analysis of hexane and ether extracts by GC-MS revealed the common occurrence of one or more phenolic hydroxyl at different locations on benzene ring. This study demonstrated the potential use of herbal extract of Carum copticum in combination therapy against ESβL producing bacteria.

Relaxant effects of aqueous cold extracts of Carum copticum on smooth muscle

Background: The objective was to study the nature of relaxant effect of cold aqueous extracts of Carum copticum on rat alimentary tract and uterus.Methods: The aqueous cold extracts of Carum copticum (omum) whole seeds (OE1) or ground seeds (OE2) was prepared by leaving them (10g to 100mL) in distilled water for 4 hrs. Rat isolated tissues like stomach, duodenum, jejunum or uterus 4 cm pieces were mounted in a 20mL bath containing Tyrode solution or De Jalons at 37°C with continuous aeration. The effect of Carum copticum extracts and thymol on spontaneous activity of intestine and on acetylcholine (ACh), Histamine (H), Barium chloride (BaCl2) induced contractions or Adrenaline produced (Adr) relaxations were observed. Thymol content of omum was estimated by HPLC.Results: Omum extracts decreased or abolished spontaneous contractions of intestine but did not antagonize the contractions induced by ACh, H or relaxation induced by adrenaline. Thymol did not show any such effect. The stimulant action of BaCl2 was antagonized by aqueous cold extracts of omum similar to Papaverine.Conclusions: The relaxant effect of Carum copticum on rat intestine appears to be a direct effect on smooth muscle like papaverine.

Larvicidal, ovicidal activities and histopathological alterations induced by Carum copticum (Apiaceae) extract against Culex pipiens (Diptera: Culicidae).

An experiment was carried out, firstly, to determine the possible toxicity of Carum copticum (Apiaceae) extract against Culex pipiens (Diptera: Culicidae), and, secondly, to study the histopathological alterations in the midgut of Cx. pipiens as a result of treatment with C. copticum extract. Larvicidal and ovicidal activities of C. copticum extract against the larvae of Cx. pipiens was determined according to World health organization (WHO). The inhibition effect of C. copticum was assessed by determining the mortality of the treated larvae and eggs. The histopathological effect of the C. copticum extracts on midgut epithelium of the larvae was examined under both light and transmission electron microscopy. The crude extract of C. copticum exerted 100% mortality for Cx. pipiens after 24 h at 200 μm/ml, and zero hatchability (100% mortality) at 150 μm/ml for Cx. pipiens. The histopathological study showed that larvae treated with C. copticum extract had cytopathological alterations of the midgut epithelium. The study provided information on various effects of C. copticum extract against Cx. pipiens.

The Effect of Ferula assa-foetida L and Carum copticum Hydroalcoholic Extract on the Expression Levels of Staphylococcus aureus Genes Involved in Quorum Sensing.

BackgroundQuorum sensing is a microbial cell-to-cell communication process. Quorum sensing bacteria produce and release extracellular messenger molecules called autoinducers. Gram-positive and Gram-negative, homoserine lactones, and oligopeptides are autoinducers used to communicate and regulate gene expression.ObjectivesThe goal of this study was to assess the impact of subinhibitory concentrations of Ferula assa-foetida l oleo-gum resin and Carum copticum fruit on the expression of tst and hld genes of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains.MethodsThis analytical study was performed using standard strains of MRSA (ATCC 33591) and MSSA (ATCC 29213). Suspensions of MRSA and MSSA bacteria were incubated at 37°C for 7 and 16 hours in the presence of ethanol extracts from F. assa-foetida and C. copticum. The expression of the hld and tst genes was then assessed using the real-time PCR protocol and SYBR Green Master Mix. The data analysis was carried out using the 2-ΔΔCT method.ResultsThe hld gene expression (RNAIII) of MRSA after 7 and 16 hours of exposure to the sMIC of the F. assa-foetida extract showed a fold change of -1 and 0.08, respectively, in comparison with controls. After 7 and 16 hours of exposure to the sMIC of the C. copticum extract, the fold change was -0.23 and -0.27, respectively. After exposure to the sMIC of the C. copticum extract for 16 hours, the fold change in the expression of the tst (TSST-1) MSSA gene was 0.37 lower than that of the control sample.ConclusionsThe results indicate that sMICs of ethanol extracts from F. assa-foetida and C. copticum can be used to control the expression of virulence genes in pathogenic bacteria, such as MRSA and MSSA.

Antibacterial activity of Carum copticum extract loaded MnFe2O4 nanoparticles coated with PEGylated chitosan

To deal with antibiotic resistance, utilization of natural antibacterial agents such as essential oils and extracts of medicinal plants seems necessary. Carum copticum is a traditional famous spice and food preservative in Indian medical system. C. copticum seeds extract have wealthy antibacterial compounds, hence to enhance stability and antibacterial activity, we loaded CE on PEGylated chitosan-MnFe2O4 NPs, which has significant antibacterial activity especially against gram negative bacteria. The nanoparticle size and crystal structure, respectively demonstrate by XRD and SEM image. CE loading analysis was investigated by FT-IR. VSM was developed to evaluate synthesized NPs magnetic properties. Releasing profile and actual CE loading were determined by UV–vis spectroscopy at (λ=275nm). Nanoparticle size was measured approximately 21nm with spherical and uniform shape. MIC of CE-PEG-chitosan-MnFe2O4 NPs were determined by liquid broth dilution method. Chitosan as biodegradable and compatible natural polymer not only has antibacterial effect, but also improves nanoparticles characteristics. We stabilized NPs with PEG against RES which increases NPs circulation in-vivo.

Comparison of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts versus systemic glucantime in the treatment of cutaneous leishmaniasis in Balb/c mice

Comparison of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts versus systemic glucantime in the treatment of cutaneous leishmaniasis in Balb/c mice

In vitro activity of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts against Leishmania major in Iran

In vitro activity of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts against Leishmania major in Iran

Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats

ObjectiveTo investigate the antidiarrhoeal activity of 95% total alcoholic extract (TAE) and total aqueous extract (TAQ) of Carum copticum (C. copticum) seeds. MethodsAntidiarrhoeal activity of C. copticum seed extracts at a dose of 100 mg/kg BW was evaluated using experimentally induced castor oil diarrhoea, gastrointestinal transit of charcoal meal and enteropooling activity in male wistar rats and compared to standard drugs. ResultsAt a dose of 100 mg/Kg BW (TAQ and TAE) significantly decreased the diarrhoeal droppings in castor oil induced diarrhoea, the mean distance travelled by charcoal meal showed a significant reduction in the secretion of gastrointestinal fluid accumulation by 39.90% to 50.70%. C. copticum extracts on castor oil induced fluid accumulation showed a greater inhibitory effect on Na+ levels than on K+ concentrations. ConclusionsThese results suggest that C. copticum seed extracts could be used for the treatment of diarrhoea.

The Effect of Carum Copticum Extract on Acetylcholine Induced Contraction in Isolated Rat's Ileum

Aims There are many biological investigations for determining an effective cure for the dysfunction of gastrointestinal tracts, using herbal medicine. It has been reported that Carum Copticum is a bactericidal agent and possesses anticholinergic, antihistaminic and b-adrenergic stimulatory effects in some tissues. However, these effects of Carum Copticum on mechanical activities of isolated intestine are not clearly identified yet. The present study has been designed to find out the specific effects of Carum Copticum on mechanical activity of isolated rat's ileum. Materials and Methods In this study rat's ileum contraction was recorded through an isolated tissue chamber in an organ bath by using isotonic transducer and oscillographic device. The effect of Carum Copticum extract on acetylcholine induced contraction in isolated rat's ileum was evaluated. Results Our findings showed that 1% aqueous extract of Carum Copticum reduces the basal contractile activity of rat's ileum. The extract also reduced acetylcholine induced contraction to 40% of its maximum response. The inhibitory action of Carum Copticum extract on acetylcholine induced contraction was similar but slower than that of atropine sulfate. Conclusion The results of this study showed an inhibitory effect of Carum Copticum extract on acetylcholine induced contraction in rat's ileum.

The analgesic effect of Carum copticum extract and morphine on phasic pain in mice

Pain is a universal complaint, which needs further investigations for new pain relieving agents. Carum copticum (L.) Sprague ex Turrill is a plant in Umbelliferae family, which is mentioned to have some therapeutic effects on headache and joint pains in Iranian traditional literature, but there are not enough scientific reports to prove its effects on pain. So, we conducted to design an experimental clinical trial study to assess and compare the analgesic effect of ethanolic extract of Carum copticum fruit with morphine by using a tail-flick analgesiometer device. Our results indicate that the test drug produced significant increase in tail-flick latency (TFL) during 2 h post-drug administration ( p < 0.05). The peak of the effect was observed at 45 min after drug injection, which was comparable to that of 1 mg/kg morphine (i.p.). Positive results in this type of analgesiometric test indicate that the antinociceptive action may be of the opoid type. The present study supports the claims of Iranian traditional medicine showing that Carum copticum extract possesses a clear-cut analgesic effect. However, further investigations are required to evaluate the efficacy and safety of this herbal medication in man.

EFFECT OF PLANT EXTRACTS ON THE ACTIVITY OF THREE PAPAYA VIRUSES

Crude leaf extract of Achyranthes aspera, Aloe barbadens, Capsicum frutescens and Carica papaya inhibit the activity of mild mosaic, distortion ringspot and ringspot viruses of papaya; Argemone mexicana and Carum copticum extracts inhibit the infectivity of DR and RS while Adenocalymna alliaceae is effective against MM only. Infectivity of all the three isolates was also inhibited by the seed extracts of Argemone mexicana, Datura fastuosa, Rhyncosia asnaris and Raphanus sativus. Seed extract of Rhyncosia asnaris which completely inhibited the infectivity of MM in vitro reduced its infectivity when sprayed on the leaves of host plants prior to inoculation or when applied as a soil drench. It was found to be stable against heat, dilution, ethyl alcohol and was partially dialysable. This seems to be of a complex nature having a protein and sugar as its components.