Copper Oxide Nanoparticles Research Articles

Toxicological assessment of the effects of CuCl2 and CuO nanoparticles on early developmental stages of the South American toad, Rhinella arenarum by standardized bioassays.

The release in aquatic environments of emergent contaminants such as copper oxide nanoparticles (CuO-NPs) has generated concerns on their short- and long-term toxicity and the potential risk for more vulnerable animal groups, such as amphibians. In this sense, the aim of this work was to evaluate the toxicity of CuO-NPs in comparison with its respective salt (CuCl2) in embryos and larvae of a native amphibian, Rhinella arenarum, by acute (96h) and chronic (504h) standardized bioassays. Lethality and sublethal effects such as developmental, morphological, and ethological alterations were assessed in a wide range of concentrations (0.001-100mg/L). Neurotoxic effects by acetyl (AChE) and butyrylcholinesterase (BChE) activity levels and changes in the lipid content were also assessed at sublethal concentrations. Results showed that CuCl2 caused higher lethality than CuO-NPs in both developmental periods. Embryos were more sensitive than larvae with LC50-96h = 0.080mg CuCl2/L and 1.26mg CuO-NPs/L and 0.21mg CuCl2/L and 20.17mg CuO-NPs/L, respectively. At acute exposure, embryos exhibited several developmental abnormalities such as developmental delay, edema, axial flexure, and microcephaly. Larvae presented spasmodic contractions and weak movements. Regarding neurotoxicity, a significant increase in AChE activity at low concentrations as well as an inhibition of BChE activity at all tested concentrations was evidenced for both substances at acute exposure. Moreover, an increment in phospholipid and triglyceride levels was observed at the highest concentration of CuO-NPs (10mg/L) at chronic exposure. The chromatographic separation of lipids showed no apparent differences in acylglycerols and free fatty acid bands, between the treatments and the control. The differences in toxicity between CuO-NPs and CuCl2 could be due to structural and physicochemical characteristics that influence their bioavailability and toxicity. Considering the exponential growth in the production and use of these substances, it is expected that the levels of contamination will rise considerably in the future, so that wildlife, particularly aquatic organisms, will be more increasingly exposed, representing a potential risk for their populations.

Ecofriendly Curcumin-Conjugated Copper Oxide Nanoparticles: Targeted Cytotoxicity and Apoptosis in Oral Carcinoma KB Cells

Ecofriendly Curcumin-Conjugated Copper Oxide Nanoparticles: Targeted Cytotoxicity and Apoptosis in Oral Carcinoma KB Cells

Twisted-tape inserts of rectangular and triangular sections in turbulent flow of CMC/CuO non-Newtonian nanofluid into an oval tube

Purpose This paper aims to study numerically the non-Newtonian solution of carboxymethyl cellulose in water along with copper oxide nanoparticles, which flow turbulently through twisted smooth and finned tubes. Design/methodology/approach The twisted-tape inserts of rectangular and triangular sections are investigated under constant wall heat flux and the nanoparticle concentration varies between 0% and 1.5%. Computational fluid dynamics simulation is first validated by experimental information from two test cases, showing that the numerical results are in good agreement with previous studies. Here, the impact of nanoparticle concentration, tube twist and fins shape on the heat transfer and pressure loss of the system is measured. It is accomplished using longitudinal rectangular and triangular fins in a wide range of prominent parameters. Findings The results show that first, both the Nusselt number and friction factor increase with the rise in the concentration of nanoparticles and twist of the tube. Second, the trend is repeated by adding fins, but it is more intense in the triangular cases. The tube twist increases the Nusselt number up to 9%, 20% and 46% corresponding to smooth tube, rectangular and triangular fins, respectively. The most twisted tube with triangular fins and the highest value of concentration acquires the largest performance evaluation criterion at 1.3, 30% more efficient than the plain tube with 0% nanoparticle concentration. Originality/value This study explores an innovative approach to enhancing heat transfer in a non-Newtonian nanofluid flowing through an oval tube. The use of twisted-tape inserts with rectangular and triangular sections in this specific configuration represents a novel method to improve fluid flow characteristics and heat transfer efficiency. This study stands out for its originality in combining non-Newtonian fluid dynamics, nanofluid properties and geometric considerations to optimize heat transfer performance. The results of this work can be dramatically considered in advanced heat exchange applications.

Preparation of jute waste-based activated carbon supported copper oxide nanoparticles for hydrogen storage in MgH2

Preparation of jute waste-based activated carbon supported copper oxide nanoparticles for hydrogen storage in MgH2

Copper oxide nanoparticles exacerbate chronic obstructive pulmonary disease by activating the TXNIP-NLRP3 signaling pathway.

Although copper oxide nanoparticles (CuONPs) offer certain benefits to humans, they can be toxic to organs and exacerbate underlying diseases upon exposure. Chronic obstructive pulmonary disease (COPD), induced by smoking, can worsen with exposure to various harmful particles. However, the specific impact of CuONPs on COPD and the underlying mechanisms remain unknown. In this study, we investigated the toxic effects of CuONPs on the respiratory tract, the pathophysiology of CuONPs exposure-induced COPD, and the mechanism of CuONPs toxicity, focusing on thioredoxin-interacting protein (TXNIP) signaling using a cigarette smoke condensate (CSC)-induced COPD model. In the toxicity study, CuONPs exposure induced an inflammatory response in the respiratory tract, including inflammatory cell infiltration, cytokine production, and mucus secretion, which were accompanied by increased TXNIP, NOD-like receptor protein 3 (NLRP3), caspase-1, and interleukin (IL)-1β. In the COPD model, CuONPs exposure induced the elevation of various indexes related to COPD, as well as increased TXNIP expression. Additionally, TNXIP-knockout (KO) mice showed a significantly decreased expression of NLRP3, caspase-1, and IL-1β and inflammatory responses in CuONPs-exposed COPD mice. These results were consistent with the results of an in vitro experiment using H292 cells. By contrast, TNXIP-overexpressed mice had a markedly increased expression of NLRP3, caspase-1, and IL-1β and inflammatory responses in CuONPs-exposed COPD mice. We elucidated the exacerbating effect of CuONPs exposure on the respiratory tract with underlying COPD, as well as related signaling transduction via TXNIP regulation. CuONPs exposure significantly increased inflammatory responses in the respiratory tract, which was correlated with elevated TXNIP-NLRP3 signaling.

Analysis of Brucine using Poly(L-Arginine) modified biosynthesized CuO nanoparticle composite sensor

The study investigated the sensitive and selective electrochemical behavior of Brucine (BC) using electrochemically polymerized L-Arginine (LA) modified with a composite comprising biosynthesized copper oxide (CuO) nanoparticles (NPs) and carbon paste electrode (PAMCuONPs/CPE). PAMCuONPs/CPE showed an optimum reversible redox-peak at 0.2 M phosphate buffer solution (PBS) at pH 2.5, allowing detection of BC. The characterization of PAMCuONPs/CPE and bare CuONPs composite carbon paste electrode (BCuONPs/CPE) was performed using scanning-electron microscopy (SEM), X-ray diffraction (XRD), and energy-dispersive spectroscopy (EDS). Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and linear sweep voltammetry (LSV) methods were employed to evaluate the performance of PAMCuONPs/CPE. Experimental parameters, including pH, and variation of concentration, were investigated. PAMCuONPs/CPE exhibited an electrochemical performance with a limit of detection (LOD) of 0.12×10−6 M and a limit of quantification (LOQ) of 0.43×10−6 M. It demonstrated good reproducibility, repeatability, and stability. PAMCuONPs/CPE was successfully validated in determining BC in real samples.

Synergistic Effect of Copper oxide Nanoparticles and Diazonium-Modified Carbon Nanotubes for Colorimetric Detection of Dimethoate Residues in Food Products

Synergistic Effect of Copper oxide Nanoparticles and Diazonium-Modified Carbon Nanotubes for Colorimetric Detection of Dimethoate Residues in Food Products

Toxicity of polystyrene nanoplastic and copper oxide nanoparticle in Artemiasalina: Single and combined effects on stress responses

Nanoparticles, such as copper oxide nanoparticle (CuO NP) and polystyrene nanoplastic (PSNP), are increasingly released into aquatic environments, and pose potential risks to aquatic animals such as brine shrimps. Understanding the toxicity of these nanoparticles, especially when combined, is very important to assess their environmental effects. Therefore, this work describes the toxicity of polystyrene nanoplastic (PSNP) and CuO nanoparticles (CuO NPs) for brine shrimp (Artemiasalina). The body length and stress biomarkers, including the activity of SOD, CAT, GST, Acid phosphatase, AChE, level of MDA and GSH, and expression of the hsp70 gene were quantified. The 48h-EC50 values for PSNP, CuO NPs, and their combination were determined as 1.024 and 5.089, and 0.512 mg L−1, respectively. The combined exposure groups showed the highest growth inhibition. This was associated with increased activity of SOD and GST, decreased activity of CAT, a significant decrease in the level of GSH, a significant increase in the MDA level, and expression of the hsp70 gene (P < 0.05). Moreover, an increased ACP and reduced AChE activity were observed in exposure groups. This study indicated that PSNP and CuO NPs have synergistic toxicity for A.salina, underscoring the importance of further investigation into their combined effect on aquatic animals.

Bimetallic peroxide nanoparticles induce PANoptosis by disrupting ion homeostasis for enhanced immunotherapy.

PANoptosis has recently emerged as a potential approach to improve the immune microenvironment. However, current methods for inducing PANoptosis are limited. Herein, through biological screening, the rational use of the nutrient metal ions Cu2+ and Zn2+ had great potential to induce PANoptosis. Inspired by these findings, we successfully developed hydrazided hyaluronic acid-modified zinc copper oxide (HZCO) nanoparticles as a PANoptosis inducer to potentiate immunotherapy. Bioactive HZCO actively delivered Cu2+ and Zn2+ while disrupting the cellular intrinsic ion metabolism pathway, resulting in double-stranded DNA release and organelle damage in cancer cells. Simultaneously, this process triggered the formation of PANoptosome and the activation of PANoptosis. HZCO-induced PANoptosis inhibited tumor growth and activated potent antitumor immune response, thereby enhancing the effectiveness of anti-programmed cell death 1 therapy. Overall, our work provides an insight into the development of PANoptosis inducers and the design of synergistic immunotherapy strategies.

Fabrication of Multifunctional Green-Synthesized Copper Oxide Nanoparticles Using Rumex vesicarius L. Leaves for Enhanced Photocatalytic and Biomedical Applications

Recently, the use of plant extracts has emerged as an innovative approach for the production of various nanoparticles. Enhancing green methods for synthesizing copper oxide (CuO) nanoparticles (NPs) is a key focus in the field of nanotechnology. This study presents a novel and eco-friendly synthesis of CuO NPs using Rumex vesicarius L. leaf extracts, offering a cost-effective and efficient method. The synthesized CuO NPs were evaluated for their cytotoxic effects against human cervical carcinoma (HeLa) cells, as well as their photocatalytic and antimicrobial activities. The morphology, size, and structural properties of the CuO NPs were characterized using various analytical techniques. X-ray diffraction (XRD) analysis confirmed the pure crystalline structure of the CuO NPs with a size of 19 nm, while transmission electron microscopy (TEM) showed particle sizes ranging from 5 to 200 nm. The photocatalytic performance of the CuO NPs was assessed through the photodegradation of crystal violet (CV) and methylene blue (MB) dyes under UV light. The NPs exhibited excellent decolorization efficiency, effectively degrading dyes in aqueous solutions under irradiation. Furthermore, the green-synthesized CuO NPs displayed strong antibacterial and antifungal activities against a variety of human pathogens. They also demonstrated significant dose-dependent cytotoxicity against the HeLa cancer cell line, with an IC50 value of 8 ± 0.54 μg/mL.

Effects of bulk forms and nanoparticles of zinc and copper oxides on the abundance, nitrogen cycling and enzymatic activities of microbial communities, morphometric parameters and antioxidant status of Hordeum vulgare L.

Uncontrolled use or improper disposal of bulk forms and nanoparticles of heavy metals may lead to their release into the environment. Coastal and floodplain ecosystems are particularly vulnerable, and the effects of metal nanoparticles on Fluvisol and Stagnic Fluvisol are poorly studied. This study aims to examine the effect of heavy metals on the enzymatic activity of the soil, the abundance of culturable microorganisms, growth, and antioxidant status of H. vulgare L. A model experiment was carried out with contamination of Stagnic Fluvisol Humic and Fluvisol with 2200 and 1320 mg kg-1 Zn and Cu, to assess the ecotoxicity of bulk forms and nanoparticles of ZnO and CuO in floodplain soils. The abundance of culturable microorganisms, namely copiotrophs, prototrophs, oligotrophs and nitrogen fixers increased. However, a sharp decrease in dehydrogenase activity and denitrification occurred. This effect was more pronounced in Fluvisol (7 times) than in Stagnic Fluvisol Humic (3 times). The accumulation of HMs was also higher in plants grown in Fluvisol (16-32 times) than in Stagnic Fluvisol Humic (13-24 times), which led to a decrease in plant growth and activation of antioxidant defense systems. An increase in the level of malondialdehyde, and the activity of superoxide dismutase and catalase indicates the induction of oxidative stress. Heavy metals have a greater impact on the biological properties of Fluvisol compared to Stagnic Fluvisol Humic. The presence of heavy metals boosts the abundance of culturable microorganisms, while nanoparticles hinder plant growth more than bulk heavy metals.

Investigation on CuO nanoparticle enhanced mahua biodiesel/diesel fuelled CI engine combustion for improved performance and emission abetted by response surface methodology

In this study, the characteristics of diesel engines were tested with in-house produced mahua biodiesel blended with diesel and copper oxide nanoparticles (CuO NP) catalyst. The preliminary investigation used mahua biodiesel-diesel blends (M10, M20, and M30) among them M20 outperformed. Further M20 and CuO NP with concentrations of 25, 50, and 75 ppm are studied. Finally, the response surface methodology (RSM) was used to determine the appropriate NP concentration for M20. The findings showed that the blend of M20 with 60 ppm NP at 80% load had the highest desirability (0.9740), and the developed RSM model predicted engine responses with a mean absolute percentage error (MAPE) of 3.0962% to the confirmation test confirming the model’s accuracy. The optimized M20NP60 blend demonstrated superior combustion, performance and emission characteristics.

Influence of CuO nanoparticles on photosystem II structural stability and functional activity of corn (Zea mays L.) under drought stress

Photoinhibition and photooxidation of photosystem II (PSII) is one of the key damages caused by drought stress. The present study aimed to assess whether or not foliar application of copper oxide nanoparticles (CuO NPs) is effective in promoting PSII stability and activity in corn plants under drought stress. Three-week old plants of corn were subjected to drought stress and varying levels of CuO nano-particles (0, 25, 50, and 100 mM) of the rooting medium. In this study, the effects of foliar application of copper oxide nanoparticles were assessed on growth, plant water status, nutrient uptake and structural stability of photosystem II of drought stressed corn plants. Drought stress impeded overall growth of the corn plants by reducing leaf relative water content, water potential, chlorophyll a content, and accumulation of K+ in plant leaves and roots. Exogenous application of 25 mM CuO NPs significantly enhanced the growth of corn. Exogenous application of CuO NPs improved the dry biomass and length of roots of the corn plants under drought stress. Although the application of nano-particles did not change leaf photosynthetic pigments, relative water content, K+ accumulation, it enhanced the accumulation of K+ in roots. Drought stress did not affect the structural stability of PSII, but it reduced its activity (performance index, PIABS) due to changes in the reaction center density and the biochemical reaction efficiency or electron transport capability. Exogenous application of CuO NPs improved PIABS due to increase in active reaction center density and electron transport efficiency. However, 100 mM CuO NPs application caused PSII damage at the donor end and reduced active reaction center density in the corn plants under both normal and drought stress conditions. The present findings provide a baseline information that foliar application of CuO-nanoparticles in low concentrations can improve the growth of corn by improving accumulation of K+ and increasing PSII activity. Further research is required to explore its effect on cellular redox balance and activities of PSII and PSI, and optimum dose of CuO-nanoparticles for developing formulations for their commercial applications in farmers’ fields.

Mechanical properties of poly(ethylene succinate) thin films incorporated with chemical synthesis copper oxide nanoparticles

This study investigates the synthesis and characterization of poly(ethylene succinate) (PES) thin films and their composites with copper oxide (CuO) nanoparticles. CuO nanoparticles were synthesized using a chemical synthesis method, achieving an average size of 20 nm. PES/CuO composites were prepared by incorporating CuO at various concentrations of 0, 1, 3, and 5 wt%. The thin films were produced through solution polymerization followed by casting. Mechanical testing revealed that the tensile strength of pure PES was 25 MPa, while incorporating 1 wt% CuO increased the tensile strength to 30 MPa. At 3 wt%, the tensile strength reached 35 MPa, and at 5 wt%, it peaked at 40 MPa, demonstrating a significant enhancement in mechanical properties. The elongation at break also improved, increasing from 200% for pure PES to 250% for the 5 wt% CuO composite. Characterization of the materials was performed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) to analyze structural and morphological properties. The results confirm that the PES/CuO composites exhibit enhanced mechanical properties, indicating their potential for diverse applications in sustainable materials and biomedical fields.

Comparison studies on the physicochemical properties and antimicrobial activities of copper (I) oxide nanoparticles synthesized using bulk and microfluidic methods

Comparison studies on the physicochemical properties and antimicrobial activities of copper (I) oxide nanoparticles synthesized using bulk and microfluidic methods

Copper Oxide Nanoparticles Impair Mouse Preimplantation Embryonic Development through Disruption of Mitophagy-Mediated Metabolism.

Copper oxide nanoparticles (CuONPs) have been widely applied, posing potential risks to human health. Although the toxicity of CuONPs on the liver and spleen has been reported, their effects on reproductive health remain unexplored. In this study, we investigate the effects of CuONPs on embryonic development and their potential mechanisms. Our results demonstrate that CuONPs exposure impairs mouse preimplantation embryonic development, particularly affecting the morula-to-blastocyst transition. Additionally, CuONPs were found to reduce the pluripotency of the inner cell mass (ICM) and mouse embryonic stem cells (mESCs). Mechanistically, CuONPs block autophagic flux and impair mitophagy, leading to the accumulation of damaged mitochondria. This mitochondrial dysfunction leads to reduced tricarboxylic acid (TCA) cycle activity and decreased α-ketoglutarate (α-KG) production. Insufficient α-KG induces the failure of DNA demethylation, reducing corresponding chromatin accessibility and consequently inhibiting ICM-specific genes expressions. Similar reduced development and inhibitions of pluripotency gene expression were observed in CuONPs-treated human blastocysts. Moreover, in women undergoing assisted reproductive technology (ART), a negative correlation was found between urinary Cu ion concentrations and clinical outcomes. Collectively, our study elucidates the mitophagy-mediated metabolic mechanisms of CuONPs embryotoxicity, improving our understanding of the potential reproductive toxicity associated with it.

Green Synthesis, Characterization, and Catalytic Applications of CuO Nanoparticles Using Prosopis Cineraria Seed Extract

Abstract The current study used a seed extract of Prosopis cineraria as a stabilizing and reducing agent to produce CuO nanoparticles via an easy, low-cost, affordable, and environmentally friendly synthesis process. The formation of copper oxide nanoparticles and the maximum absorbance of the CuO nanoparticles produced in the solution at 565 nm were verified by UV-vis. Copper oxide nanoparticles were found to have secondary metabolites on their surface, as shown by a distinctive Cu-O stretching band at 532 cm−1, which confirmed the reduced Cu2+ ions in copper oxide nanoparticles. This was confirmed by FTIR analysis. The XRD analysis confirmed the produced copper oxide nanoparticles’ monoclinic crystalline nanostructure with an average particle size of 34 nm. The phytonutrients in Prosopis cineraria seed extract stabilized and reduced copper, as demonstrated by the existence of copper and oxygen atoms at 85.2% and 12.5%, respectively, as demonstrated by SEM-EDX analysis. According to the HR-TEM study, copper oxide nanoparticles with a mean size of 18 nm are spherical in shape and well distributed. Prosopis cineraria seed extract-derived copper oxide nanoparticles were utilized as a catalyst in the Ullmann process to produce diphenyl ether. CuO nanoparticles produced by Prosopis cineraria seed extraction as a catalyst yielded 91% diphenyl ether. The results showed that a more ecologically friendly way of synthesizing copper oxide nanoparticles with great homogeneity of particle sizes could be achieved using seed extract. This work aims to facilitate heterogeneous catalysis from CuO nanoparticles utilising Prosopis cineraria seed extract. Overall, this technique offers several advantages, like high yields at fast reaction times, and low catalyst loading are just a few of this approach’s many benefits.

Multifunctional scaffolds of β-tricalcium phosphate/bioactive glass coated with zinc oxide and copper oxide nanoparticles

Incorporating nanoparticles into scaffolds with regenerative potential is a promissory strategy to provide them with antimicrobial activity. Bioceramics, such as β-tricalcium phosphate (β-TCP) and bioactive glasses (BGs), stand out among synthetic materials for bone regeneration. In this context, we report the incorporation of zinc oxide (ZnO) and copper oxide/copper nitrate (CuO/Cu2H3NO5) nanoparticles onto the surface of the β-TCP/BG scaffolds. This report addresses the physicochemical characterization of the scaffolds, their antimicrobial activity, and their response to MC3T3-E1 cells. Our findings show that the incorporation of both nanoparticles effectively inhibited S. aureus growth, including its biofilm formation. While the presence of the nanoparticles initially decreased MC3T3-E1 cell viability, cell proliferation improved with prolonged incubation. Overall, the β-TCP/BG_Zn and β-TCP/BG_Cu scaffolds showed an early antimicrobial response, aiding infection eradication, while also supporting cell proliferation over time.

Tecoma stans intermediated green synthesis of copper oxide nanoparticles, its characterization, paracetamol degradation and biological activities

The main objective of the present research work is to green synthesize copper oxide nanoparticles (CuO NPs) and to study its potential against various enmities to safeguard our environment and health. Based on this approach, preparation of CuO NPs was accomplished using four different volumes (10, 25, 40, and 50 mL of leaves extract) of Tecoma stansleaves extract. Moreover, all the synthesized CuO NPs were characterized using XRD, UV–visible, FTIR, and SEM with EDS analysis. From the XRD pattern, the structures of all the synthesized CuO NPs were found to be monoclinic in phase. According to the UV–visible studies, the calculated band gap energy values for all the prepared CuO NPs were higher compared to bulk CuO, which was 1.2 eV. Further, the FTIR results showed the presence of CuO bond and SEM with EDS analysis revealed the surface morphology of CuO NPs andthe presence of elements showing the purity of synthesized CuO NPs, respectively. The optimized condition for the preparation of CuO NPs were 50 mL of leaves extract mixed with 50 mL of double distilled water (CuO4 NPs) showed a spheroidal morphology as evident from the HRTEM images. XPS analysis used to find the oxidation state of detected elements especially for copper and oxygen. The predicted BET and Langmuir surface area of CuO4 NPs were calculated to be 40.305 m2/g and 5074.12 m2/g respectively. Photocatalytic degradation of paracetamol (PCM) using CuO4 NPs was investigated, and the effect of parameters such as pH, initial concentration of PCM solution, and catalyst dosage were studied under UV light irradiation. The highest removal of 95.15 % had been achieved at 120 min under optimized reaction conditions. Additionally, CuO4 NPs also exhibited exemplary antibacterial activity against bacillus subtilis bacteria with a zone of inhibition of 26 mm. Further, in vitro cytotoxic behaviour of CuO4 NPs was estimated using A549 cancer cells by MTT assay. Thus, the green synthesis of CuO NPs using Tecoma stans leaves extract has the capacity to participate in photocatalytic and biological activities.

Lemon Peel‐Extracted Recyclable Copper Oxide Nanoparticles for Methylene Blue Dye Adsorption: Optimization, Isotherms, Kinetics, Thermodynamics, and Reusability Study

AbstractResearchers are exploring sustainable methods for synthesizing metal oxide nanoparticles to address wastewater pollution, which poses a global threat to aquatic life and human health. The present research aims to biosynthesize recyclable Lemon peel‐extracted copper oxide nanoparticles (CuO NPs) to remove methylene blue dye via adsorption. Our approach involves a comprehensive range of techniques, such as FTIR, XRD, SEM‐EDX, Zeta potential, and UV‐VIS, to thoroughly characterize the NPs. SEM analysis confirmed that the CuO NPs, with an average size of 64.5 nm and a nearly spherical shape, exhibited a zeta potential value of – 16.34, indicating their relative stability. Optimized conditions for the 92.20% adsorption efficiency for methylene blue dye were pH at 6, contact time of 90 min, 0.06 g of adsorbent dose, 10 ppm dye conc., and Temp. 27 °C. Langmuir 1‐based maximum adsorption capacity (qm) was 617.28 mg/g for CuO NPs. Langmuir 1 and Freundlich are the most suitable isotherm models for experimental data, supporting Physi‐chemisorption and chemisorption as rate‐limiting steps in the exothermic adsorption process. A reusability study of up to three cycles proved the sustainability of the materials.