Aim: To develop an optimized approach for encapsulating a 2-alkylthioimidazolone-based copper coordination compound within liposomes, which could offertreatment of cancer and bacterial infectionsby reactive oxygen species generation toxicity mechanisms. Materials & methods: For drug-loaded liposome preparation, lipidsand drug mixturein organic solvents wasinjected into copper salt solution, forming a coordination compound simultaneouslyembedded in the lipid bilayer. In vitro tests were performed on MCF7 and MDA-MB-231 breast cancer cells. Results: Liposomes had a loading capacity of up to 1.75% (molar drug-to-lipid ratio). In vitro tests showed increased viability and accumulation of the liposomal formulation compared with free drugas well as lack of cytotoxicity in hepatocytes. Conclusion: This optimized technique for encapsulating large copper complexes in liposomes could be used to improve theirdelivery and better treat cancer and bacterial infections.