Event Abstract Back to Event Cutaneous candidiasis in BALB/c mice Tacito G. Campois1*, Amanda Z. Zucoloto1, Gustavo F. Quirino1, Luiz A. Custodio1, Ivete C. Costa1, Eduardo J. Araújo1, Terezinha I. Svidzinski2 and Ionice Felipe1 1 Universidade Estadual de Londrina, Brazil 2 Universidade Estadual de Maringá, Brazil Individuals with diabetes mellitus are predisposed to cutaneous candidiasis. BALB/c mice were chosen as model of chronic cutaneous candidiasis because in further studies, this mycosis will be studied in diabetic BALB/c. C. albicans pseudohyphae (5X106) were injected in the deep dermis of the hindpaw and PBS in the contralateral paw. After 0, 1, 4, 7,14 and 21 days of infection the mice were euthanized and tissues injured by infection and the popliteal lymph nodes collected for histopathological and cytokines analysis. The extent of the inflammatory area was evaluated daily with a paquimeter. The peak of inflammatory area occurred at day 6 of infection (2.64 ± 0.57 mm), persisted until day 9 (2.70 ± 0.54 mm), reducing significantly in size until day 15 as compared to day 9. C. albicans caused significant thickening of the epidermis and induced migration of inflammatory cells to the lesion site. IFN-g, IL-6, TNF-a and IL-10 were found in low levels at the infection sites, whereas the immune response at the popliteal lymph nodes was intense: the levels of IFN-g (994±54 pg) and TNF-a (437±31pg) were high until day 4 of infection; those of IL-6 were high (520±8pg) until day 4 and those of IL-10 remained low in the very initial phase and increased from day 4 on. These cytokines at these levels could play important roles in this model of cutaneous candidiasis: IFN-g and TNF-a in Candida antigens processing and presentation and therefore fungal clearance, IL-6 in neutrophils migration and IL-10 in lesion repair. Acknowledgements Fundação Araucária, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). References KAGAMI, S.; RIZZO, H. L.; KURTS, S.E.; MILLER, L.S.; BLAUVELT, A. IL-23 and IL-17 A, but not IL12 and IL22, are required for optimal skin host defense against Candida albicans. J Immunol. 2010; 185: 5453-5462. Keywords: Cutaneous candidiase, Cytokines, Inflammation, injury, mouse models Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Campois TG, Zucoloto AZ, Quirino GF, Custodio LA, Costa IC, Araújo EJ, Svidzinski TI and Felipe I (2013). Cutaneous candidiasis in BALB/c mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00241 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Tacito G Campois, Universidade Estadual de Londrina, Londrina, Brazil, tacito@biologo.bio.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tacito G Campois Amanda Z Zucoloto Gustavo F Quirino Luiz A Custodio Ivete C Costa Eduardo J Araújo Terezinha I Svidzinski Ionice Felipe Google Tacito G Campois Amanda Z Zucoloto Gustavo F Quirino Luiz A Custodio Ivete C Costa Eduardo J Araújo Terezinha I Svidzinski Ionice Felipe Google Scholar Tacito G Campois Amanda Z Zucoloto Gustavo F Quirino Luiz A Custodio Ivete C Costa Eduardo J Araújo Terezinha I Svidzinski Ionice Felipe PubMed Tacito G Campois Amanda Z Zucoloto Gustavo F Quirino Luiz A Custodio Ivete C Costa Eduardo J Araújo Terezinha I Svidzinski Ionice Felipe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.