The fluctuating heat sources and seasonal condensing environments are critical to the operation of Organic Rankine Cycle (ORC) under off-design conditions. This paper presents a comprehensive steady-state mathematical model developed using a newly built experimental platform. Focusing on the operational characteristics, including the evaporation pressure, condensation temperature, mass flow rate, and the performance of key equipment during the regulation of the expander, working fluid pump, and cooling water pump. Results show that the net efficiency initially increases before decreases, with both evaporation pressure and mass flow rate rising alongside the working fluid pump frequency. The quasi-two-stage single screw expander achieves an isentropic efficiency above 65 %. Additionally, flexible adjustments to the cooling water pump effectively regulate the cooling system, with timely cleaning of cooling pipelines enhancing net efficiency by up to 3.27 %. Optimization using the particle swarm algorithm improves system performance, achieving net efficiency enhancements of 1.8 % and 12.3 % under specific conditions. The novelty of this study lies in directly regulation of the expander, working fluid pump, and cooling water pump, significantly enhancing the off-design performance. This research provides valuable insights for researchers and designers, enabling flexible regulation of ORC operations to ensure efficient performance under varying conditions.