ABSTRACT THE purpose of this research was to study the application of electrostatic principles in removing flower parts from onion seeds. The optimal machine and environmental factors that would cause separation of flower parts from an onion seed/flower part mixture were determined. The electrostatic separator had a beam type electrode, conducting type conveyor belt, and a 25 kVDC power supply. The chemical contents of the seeds and the flower parts were determined. The germination percentages and the densities of the seeds in the different separated fractions were determined. The electrostatic separator, at 17 kV in the pinning position, was effective in separating flower parts from onion seeds. Humidities less than 40% and temperatures less than 24 C were found to be essential for effective separation of flower parts from onion seeds. The onion seeds had higher protein dry weight, crude fat, acid detergent fiber content but lower ash and nitrogen-free extract than the flower parts. The electrostatic separator was capable of separating seeds based on density. Seeds having higher density had higher germination.