Type Conversion Research Articles

Tunable multicolor supramolecular assemblies based on phosphorescence cascade energy transfer for photocatalytic organic conversion and anti-counterfeiting

Making full use of the captured energy by phosphorescence light-harvesting systems (PLHSs) and the tunable photoluminescence in energy transfer process to realize the multiple applications is still the challenge of PLHSs research. In this study, we have successfully constructed a highly effective PLHS with tunable multicolor luminescence and efficient conversion of photosensitizer types, which can further be used in photocatalytic organic conversion, information anti-counterfeiting and storage. The supramolecular polymer of BDBP-CB[8], which is generated by cucurbit[8]uril (CB[8]) and 4-(4-bromophenyl)-pyridine derivative (BDBP), realizes a phosphorescence emission and a change in luminescence color. Notably, white light emission was achieved and the logic gate systems were constructed utilizing the application of adjustable luminescence color. More interestingly, PLHS can be constructed by employing BDBP-CB[8] as energy donors, Sulforhodamine 101 (SR101) and Cyanine5 (Cy5) as energy acceptors, which results in a remarkably tunable multicolor photoluminescence to achieve the information storage. Furthermore, we have also found that BDBP-CB[8] can serve as type II photosensitizer for the effective production of singlet oxygen (1O2) during the photooxidation process of styrene in aqueous environments, attaining a remarkable output rate reaching as high as 89 %. Particularly, compared with 1O2 produced by type II photosensitizer BDBP-CB[8], the construction of PLHS can effectively convert type II photosensitizer to type I photosensitizer and efficiently generate superoxide anion radical (O2•−), which can be used for photocatalytic cross-dehydrogenative coupling (CDC) reaction in the aqueous solution with a yield of 90 %. Thus, we have created a PLHS that not only achieves tunable multicolor emission for information anti-counterfeiting and storage, but also realizes the conversion of reactive oxygen species (ROS) for different types photocatalytic oxidation reactions.

Nano-Se exhibits limited protective effect against heat stress induced poor breast muscle meat quality of broilers compared with other selenium sources

BackgroundAt present, heat stress (HS) has become a key factor that impairs broiler breeding industry, which causes growth restriction and poor meat quality of broilers. Selenium (Se) is an excellent antioxidant and plays a unique role in meat quality improvement. Recent years, nano-selenium (NanoSe) has received tremendous attention in livestock production, due to its characteristic and good antibacterial performance in vitro. Here, we developed the heat stressed-broiler model to investigate the protective effects of NanoSe on growth performance and meat quality of broilers and compare whether there are differences with that of other Se sources (Sodium selenite, SS; Selenoyeast, SeY; Selenomethionine, SeMet).ResultsHS jeopardized the growth performance and caused poor meat quality of breast muscle in broilers, which were accompanied by lowered antioxidant capacity, increased glycolysis, increased anaerobic metabolism of pyruvate, mitochondrial stress and abnormal mitochondrial tricarboxylic acid (TCA) cycle. All Se sources supplementation exhibited protective effects, which increased the Se concentration and promoted the expression of selenoproteins, improved the mitochondrial homeostasis and the antioxidant capacity, and promoted the TCA cycle and the aerobic metabolism of pyruvate, thus improved the breast muscle meat quality of broilers exposed to HS. However, unlike the other three Se sources, the protective effect of NanoSe on meat quality of heat stressed-broilers was not ideal, which exhibited limited impact on the pH value, drip loss and cooking loss of the breast muscle. Compared with the other Se sources, broilers received NanoSe showed the lowest levels of slow MyHC, the highest levels of fast MyHC and glycogen, the highest mRNA levels of glycolysis-related genes (PFKM and PKM), the highest protein expression of HSP60 and CLPP, and the lowest enzyme activities of GSH-Px, citroyl synthetase (CS) and isocitrate dehydrogenase (ICD) in breast muscle. Consistent with the SS, the Se deposition in breast muscle of broilers received NanoSe was lower than that of broilers received SeY or SeMet. Besides, the regulatory efficiency of NanoSe on the expression of key selenoproteins (such as SELENOS) in breast muscle of heat stressed-broilers was also worse than that of other Se sources.ConclusionThrough comparing the meat quality, Se deposition, muscle fiber type conversion, glycolysis, mitochondrial homeostasis, and mitochondrial TCA cycle-related indicators of breast muscle in heat stressed broilers, we found that the protective effects of organic Se (SeY and SeMet) are better than that of inorganic Se (SS) and NanoSe. As a new Se source, though NanoSe showed some protective effect on breast muscle meat quality of heat stressed broilers, the protective effect of NanoSe is not ideal, compared with other Se sources.

Bridging the Gap: Establishing Spatial Correlations and Type Conversion Relationships among Different Aerosol Idetification Methods

Effective identification of aerosol type is the basis of accurate quantification of aerosol-climate and aerosol-environment interactions. However, there are different aerosol category settings and identifying parameter selections among different aerosol identification methods, which causes incoherence in data comparisons and uncertainty in quantifying thier climate and environment effects. To bridge the gap, this study establishes spatial correlations and aerosol type conversion relationships among four commonly-used aerosol identification methods, which includes AOD-AE, AE-SSA, FMF-SSA, and SAE-AAE. Aerosol optical properties observed at 37 typical AERONET sites are selected as the testbed, including marine, desert dust, clean continent, urban industry, and biomass burning sites. Results show that marine and desert dust aerosols are the most effectively identified types, while urban industry and biomass burning aerosols overlap much in the identification spaces since they contain similar chemical components. Dust and marine aerosol generally have the best agreement among the four identification methods, because desert dust and marine particles have distinctive chemical components from other kinds, thus the category setting from the perspective of emission source, light absorbing ability, or chemical components has less influence on the identification results. Spatial correlation between the four commonly-used aerosol identification methods futher refines the correspondence of aerosol category, light absorption capacity, and chemical components. And we find that uncertain aerosols defined in FMF-SSA are mostly marine aerosols.

Innate lymphoid cell-based immunomodulatory hydrogel microspheres containing Cutibacterium acnes extracellular vesicles for the treatment of psoriasis

Psoriasis is a chronic skin inflammation influenced by dysregulated skin microbiota, with the role of microbiota in psoriasis gaining increasing prominence. Bacterial extracellular vesicles (bEVs) serve as crucial regulators in the interaction between hosts and microbiota. However, the mechanism underlying the therapeutic potential of bEVs from commensal bacteria in psoriasis remains unclear. Here, we investigated the therapeutic role of Cutibacterium acnes (C. acnes)-derived extracellular vesicles (CA-EVs) in psoriasis treatment. To prolong the active duration of CA-EVs, we encapsulated them in gelatin methacrylate (GelMA) to fabricate hydrogel microspheres (CA-EVs@GHM) with sustained release properties. As GelMA degraded, CA-EVs were gradually released, maintaining a high concentration in mouse skin even 96 h post-treatment. In human keratinocyte cells (HaCaT), CA-EVs@GHM enhanced resistance to Staphylococcus aureus (S. aureus), promoted proliferation and migration of HaCaT cells exposed to S. aureus, and significantly reduced the expression of inflammatory genes such as interleukin (IL)-6 and C-X-C motif chemokine ligand 8 (CXCL8). In vivo, CA-EVs@GHM, more potent than CA-EVs alone, markedly attenuated proinflammatory gene expression, including tumor necrosis factor (TNF), Il6, Il17a, Il22 and Il23a in imiquimod (IMQ)-induced psoriasis-like mice, and restored skin barrier function. 16S rRNA sequencing revealed that CA-EVs@GHM might provide therapeutic effects against psoriasis by restoring microbiota diversity on the back skin of mice, reducing Staphylococcus colonization, and augmenting lipid metabolism. Furthermore, flow cytometry analysis showed that CA-EVs@GHM prevented the conversion of type 2 innate lymphoid cells (ILC2) to type 3 innate lymphoid cells (ILC3) in psoriasis-like mouse skin, reducing the pathogenic ILC3 population and suppressing the secretion of IL-17 and IL-22. In summary, our findings demonstrate that the long-term sustained release of CA-EVs alleviated psoriasis symptoms by controlling the transformation of innate lymphoid cells (ILCs) subgroups and restoring skin microbiota homeostasis, thus offering a promising therapy for psoriasis treatment. Statement of significanceCutibacterium acnes, which is reduced in psoriasis skin, has been reported to promote skin homeostasis by regulating immune balance. Compared to live bacteria, bacterial extracellular vesicles (bEVs) are less prone to toxicity and safety concerns. bEVs play a pivotal role in maintaining bacterial homeostasis and modulating the immune system.However,bEVs without sustained release materials are unable to function continuously in chronic diseases. Therefore, we utilized hydrogel microspheres to encapsulate Cutibacterium acnes (C. acnes)-derived extracellular vesicles (CA-EVs), enabling long term sustained release. Our findings indicate that, CA-EVs loaded gelatin methacrylate hydrogel microspheres (CA-EVs@GHM) showed superior therapeutic effects in treating psoriasis compared to CA-EVs. CA-EVs@GHM exhibited a more significant regulation of pathological type 3 innate lymphoid cells (ILC3) and skin microbiota, providing a promising approach for microbiota-derived extracellular vesicle therapy in the treatment of skin inflammation.

Spatio-temporal Evolution and Prediction of Carbon Storage in Huaibei City Based on InVEST-PLUS Model

This study aimed to investigate the impact of spatiotemporal changes in land use on ecosystem carbon storage. The study analyzed the spatiotemporal changes in carbon storage in the study area based on land use data from five periods (1985, 1995, 2005, 2015, and 2020) using the InVEST model. The PLUS model was used to predict land use changes in the study area under four different scenarios (natural development, farmland protection, ecological protection, and double protection of farmland and ecology) in 2035, and the ecosystem carbon storage under different scenarios was estimated. The results of the study indicated that the farmland in the area under investigation had been decreasing consistently from 1985 to 2020, with a more rapid rate of change observed between 2015 and 2020. During this period, the overall dynamic attitude towards land use reached 34.62 %. Additionally, the carbon storage in the area showed a decreasing trend over the years, with a decrease of 1.55×105 t from 1985 to 2020. Between 2005 and 2015, the carbon storage showed a decrease of 1.22×105 t, with an average annual decrease of 1.22×104 t. The areas with higher carbon storage were located in the eastern part of the study area, whereas areas with lower carbon storage were found in the central and northwestern parts. Although the proportion of carbon storage in farmland decreased from 66.89 % to 57.73 %, farmland remained the most important carbon pool in the study area. The conversion of other land use types to grassland and forestland was advantageous for increasing ecosystem carbon storage. Finally, the study projected that by 2035, the carbon storage in the natural development scenario, the farmland protection scenario, the ecological protection scenario, and the dual protection scenario would be 81.77×105, 82.45×105, 82.82×105, and 82.51×105 t, respectively.

A mechanical manipulated electromechanical coupling design with stretchable electret film: mechanical sensing, energy harvesting, and actuation

Electrical and mechanical energy converts around the nature, and electromechanical coupling effect is applied in various conditions such as mechanical sensing, electrical actuation, and self-powering. During the energy type conversion, electromechanical parameters are among the key issues, such as enlarging the sensitivity and range of mechanical sensing, and energy harvesting efficiency. In this work, a mechanical manipulated approach with stretchable electret is proposed to continuously manipulate the electromechanical parameters. An electromechanical coupling demonstration with pre-stretched electret films and non-contact electrodes are applied, verifying high and regulable electromechanical coupling parameters, and it is advantaged from large deformable and overload permissible capability. This mechanical manipulation approach proposes a new possibility on simplifying the structural and mechanical design of various electromechanical devices, and further enhancing the general applicability with certain geometry and material with ultra-high and tunable electromechanical coupling parameters.

Non-amphoteric N-type doping with Sn of GaAs(631) layers grown by molecular beam epitaxy

The Sn-doping effects on the electrical conduction and optical properties of GaAs(631)A epilayers grown by molecular beam epitaxy were investigated. We found that the conduction type conversion, frequently observed in the doping of layers grown on high-index substrates, is avoided when tin-doping is implemented. The maximum free-carrier concentration (n) obtained in GaAs(631):Sn was 2 × 1019 cm−3, an order of magnitude higher than previously reported for GaAs(631):Si, and within the same order of magnitude for the growth of GaAs(100):Si. The electron mobility was suitable for many optoelectronic applications. Raman spectroscopy showed low lattice disorder in (631) oriented samples, compared with singular (100) samples. The photoluminescence characterization of the samples revealed the blueshift of the optical transitions close to E0 associated with the Moss–Burstein effect for Sn doping. Photoreflectance spectroscopy was used to study the doping properties at the critical points E1 and E1 + Δ1, where the major affectation with n was perceived in the broadening parameter Γ.

Multi-objective genetic algorithm embedded with reinforcement learning for petrochemical melt-flow-index production scheduling

In the petrochemical industry, product type conversion can cause capacity loss and produce transition products, leading to waste. Our study aims to minimize these transition products and tardiness in meeting customer due dates. We introduce the Engineering Experience Heuristic (EEH), which utilizes scheduling reference sheets to identify key production traits and constraints, thereby generating efficient schedules. Further building on the EEH, we propose a non-dominated sorting genetic algorithm II enhanced with reinforcement learning (NSGAeRL) to address the multi-objective scheduling optimization problem. The incorporation of reinforcement learning (RL), particularly through a model-based Markov Decision Process (MDP), allows for the dynamic adjustment of genetic algorithm (GA) parameters, including crossover and mutation mechanisms, based on the optimal policy derived from RL. We validate our proposed methodologies through empirical studies and numerical experiments. The results demonstrate that NSGAeRL outperforms standard benchmarking algorithms, offering superior performance in minimizing transition products and scheduling tardiness.

Grape seed proanthocyanidin extract promotes skeletal muscle fiber type transformation through modulation of cecal microbiota and enhanced butyric acid production.

The conversion of fast-twitch fibers into slow-twitch fibers within skeletal muscle plays a crucial role in improving physical stamina and safeguarding against metabolic disorders in individuals. Grape seed proanthocyanidin extract (GSPE) possesses numerous pharmacological and health advantages, effectively inhibiting the onset of chronic illnesses. However, there is a lack of research on the specific mechanisms by which GSPE influences muscle physiology and gut microbiota. This study aims to investigate the role of gut microbiota and their metabolites in GSPE regulation of skeletal muscle fiber type conversion. In this experiment, 54 male BALB/c mice were randomly divided into three groups: basal diet, basal diet supplemented with GSPE, and basal diet supplemented with GSPE and antibiotics. During the feeding period, glucose tolerance and forced swimming tests were performed. After euthanasia, samples of muscle and feces were collected for analysis. The results showed that GSPE increased the muscle mass and anti-fatigue capacity of the mice, as well as the expression of slow-twitch fibers. However, the beneficial effects of GSPE on skeletal muscle fibers disappeared after adding antibiotics to eliminate intestinal microorganisms, suggesting that GSPE may play a role by regulating intestinal microbial structure. In addition, GSPE increased the relative abundance of Blautia, Muribaculaceae, and Enterorhabdus, as well as butyrate production. Importantly, these gut microbes exhibited a significant positive correlation with the expression of slow-twitch muscle fibers. In conclusion, supplementation with GSPE can increase the levels of slow-twitch fibers by modulating the gut microbiota, consequently prolonging the duration of exercise before exhaustion. PRACTICAL APPLICATION: This research suggests that grape seed proanthocyanidin extract (GSPE) has potential applications in improving physical stamina and preventing metabolic disorders. By influencing the gut microbiota and increasing butyric acid production, GSPE contributes to the conversion of fast-twitch muscle fibers into slow-twitch fibers, thereby enhancing anti-fatigue capacity and exercise endurance. While further studies are needed, incorporating GSPE into dietary supplements or functional foods could support individuals seeking to optimize their exercise performance and overall metabolic health.

Paradoxical reaction to IL-17A inhibitor: a case report and literature review.

A case of pustular psoriasis after treatment with secukinumab in a patient with plaque psoriasis is reported, which is the first case in China. To summarize the clinical characteristics of patients who developed the rare paradoxical reaction and treatment options received IL-17A antagonist therapy, we conducted a further literature review. Data were analyzed from a patient with plaque psoriasis who developed pustular psoriasis after treatment with secukinumab. A comprehensive review of relevant domestic and international literature was conducted, focusing on cases that met our inclusion criteria for analysis and synthesis. Anti IL-17A therapy may lead to type conversion, with reported cases more prevalent in women and varying in onset time, predominantly involving palmoplantar pustulosis. Given the increasing use of IL-17A antagonists in psoriasis treatment, it is crucial to monitor for rare adverse reactions, including the paradoxical induction of pustular psoriasis.

The pathway to curb greenwashing in sustainable growth: The role of artificial intelligence

Artificial Intelligence (AI) can improve production efficiency and general quality of life through assisting human labor, potentially leading to the conversion of employment types, enhancing industrialization, and upgrading energy structure. This paper enriches the role of AI in improving sustainable growth by curbing hypocritical sustainable and greenwashing behaviors. By accessing the panel data from Chinese listed-firms for the period 2014–2021, we have shown that AI can significantly mitigate the existence of greenwashing behaviors by raising the disclosure quality of ESG rating scores. Moreover, the role of AI in mitigating greenwashing behaviors performs significantly in SOEs, less pollution-intensive industries, high environmental regulation and less developed green finance regions. Furthermore, the potential mechanisms of AI in mitigating greenwashing behaviors are displayed, including alleviating financial constraints, easing management cost, improving green innovations.

ANALISA POTENSI KECEPATAN ANGIN PADA MODUL LATIH RANCANG BANGUN PLTB

Electrical energy is energy that can be generated from the conversion of various types of energy, one of which is wind energy. The average wind speed in Indonesia is classified as low speed, only certain areas have moderate to high wind speeds. Low wind speed does not mean that the potential energy contained in it cannot be utilized or converted into electrical energy, it can still be utilized but a generator is needed that matches the characteristics of the wind speed. Therefore, the turbine used is the Savonius type, which can work in low wind speeds. The purpose of this research is to measure output power against wind speed. With experimental methods using an anemometer and measuring voltage, current and output power. From the results of this study, the maximum power generated by the wind power training module is 0.008 Watt with a wind speed of 4.31 m/s and the minimum power generated is 0.001 Watt with a wind speed of 1.17 m/s. In a day, the average wind speed is 2.94 m/s so from the wind speed the wind power training module is able to produce an average power of 0.001 Watt.

Multimedia healthcare cloud personal archives security system based on compressed sensing and multi-image encryption

Cloud services in the medical process have become commonplace, and the semi-trusted nature of the healthcare cloud makes security in the communication process particularly important. This paper summarizes the four major pain points in the process of collecting, storing in the cloud, and communicating information in the medical process, and proposes a multimedia medical cloud information security system. The pressure of channel transmission is reduced by compressing two-dimensional information images with compressed sensing (CS) technology. The multimedia information type conversion technology can convert multimedia information into 2D images, and the converted 2D images are merged into a multi-image cube, which realizes the “one-person-one-cube” medical information archives management mode. A chaotic multi-image encryption (MIE) based on Identity Mutual Recognition Keys (IMRKs) is designed to encrypt the cube by transmitting it to the edge cloud, which ensures security and solves the problem of unauthorized use of medical information. The 3D Positioning Fifth Generation Message-Digest (3DPMD5) tamper determination algorithm is utilized to generate 32-bit hexadecimal numbers that are transmitted back to the medical terminal to achieve cloud tamper resistance. It is able to be demonstrated from the simulation results that the system has good reconstruction results, relatively safe performance, and high encryption and decryption efficiency.

Spike structure of gold nanobranches induces hepatotoxicity in mouse hepatocyte organoid models

BackgroundGold nanoparticles (GNPs) have been extensively recognized as an active candidate for a large variety of biomedical applications. However, the clinical conversion of specific types of GNPs has been hindered due to their potential liver toxicity. The origin of their hepatotoxicity and the underlying key factors are still ambiguous. Because the size, shape, and surfactant of GNPs all affect their properties and cytotoxicity. An effective and sensitive platform that can provide deep insights into the cause of GNPs’ hepatotoxicity in vitro is therefore highly desired.MethodsHere, hepatocyte organoid models (Hep-orgs) were constructed to evaluate the shape-dependent hepatotoxicity of GNPs. Two types of GNPs with different nanomorphology, gold nanospheres (GNSs) and spiny gold nanobranches (GNBs), were synthesized as the representative samples. Their shape-dependent effects on mice Hep-orgs’ morphology, cellular cytoskeletal structure, mitochondrial structure, oxidative stress, and metabolism were carefully investigated.ResultsThe results showed that GNBs with higher spikiness and tip curvature exhibited more significant cytotoxicity compared to the rounded GNSs. The spike structure of GNBs leads to a mitochondrial damage, oxidative stress, and metabolic disorder in Hep-orgs. Meanwhile, similar trends can be observed in HepG2 cells and mice models, demonstrating the reliability of the Hep-orgs.ConclusionsHep-orgs can serve as an effective platform for exploring the interactions between GNPs and liver cells in a 3D perspective, filling the gap between 2D cell models and animal models. This work further revealed that organoids can be used as an indispensable tool to rapidly screen and explore the toxic mechanism of nanomaterials before considering their biomedical functionalities.

Conversion of silicon wafer type from perfect-interstitial to perfect-vacancy by rapid thermal process

Vacancies, self-interstitials, and Frenkel pairs represent the basic point defects in silicon single-crystals and are unavoidably introduced in the ingots during the manufacturing process. In semiconductor technology, several problems are caused by points defects that are not at thermodynamic equilibrium. For this reason, virtually defect-free silicon wafers having a small residual concentration of vacancies in the bulk (“Perfect-vacancy”, or Pv silicon) are solely used for the fabrication of advanced microelectronic devices, whereas similar wafers containing a residual concentration of silicon self-interstitials (Pi silicon) are often regarded as unsuitable substrates for device fabrication. This paper describes the possibility of using Pi silicon wafers, after their conversion to Pv, for those applications requiring only vacancy-rich perfect silicon as the substrate. The conversion from Pi to Pv is achieved in these wafers by using rapid thermal annealing at high temperature to produce Frenkel pairs first and to re-install a specific vacancy concentration in the bulk of the wafer later, thus reassigning a new Pv character to the silicon substrate.

Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle.

Skeletal muscle plays critical roles in providing a protein source and contributing to meat production. It is well known that microRNAs (miRNAs) exert important effects on various biological processes in muscle, including cell fate determination, muscle fiber morphology, and structure development. However, the role of miRNA in skeletal muscle development remains incompletely understood. In this study, we observed a critical miRNA, miR-24-3p, which exhibited higher expression levels in Tongcheng (obese-type) pigs compared to Landrace (lean-type) pigs. Furthermore, we found that miR-24-3p was highly expressed in the dorsal muscle of pigs and the quadriceps muscle of mice. Functionally, miR-24-3p was found to inhibit proliferation and promote differentiation in muscle cells. Additionally, miR-24-3p was shown to facilitate the conversion of slow muscle fibers to fast muscle fibers and influence the expression of GLUT4, a glucose transporter. Moreover, in a mouse model of skeletal muscle injury, we demonstrated that overexpression of miR-24-3p promoted rapid myogenesis and contributed to skeletal muscle regeneration. Furthermore, miR-24-3p was found to regulate the expression of target genes, including Nek4, Pim1, Nlk, Pskh1, and Mapk14. Collectively, our findings provide evidence that miR-24-3p plays a regulatory role in myogenesis and fiber type conversion. These findings contribute to our understanding of human muscle health and have implications for improving meat production traits in livestock.

Evolution of point defects in mechanical cracks of fused silica after CO2 laser melting

The traditional polishing method inevitably results in subsurface cracks in the fused silica, which seriously degrades their ultraviolet laser damage resistance. CO2 laser irradiation can melt these cracks and improve their laser induced damage threshold (LIDT). Photoluminescence spectrum and SEM-FIB were employed to investigate the changes in the material microstructure at the crack location with CO2 laser melting. The density of the oxygen-deficient centers of type II (ODC II) defects decreases, while the density of the non-bridging oxygen hole center (NBOHC) defects increases after high-temperature melting. The reason for this change is related to the dihydroxylation reaction and the participation of environmental oxygen in the defect type conversion. The reduction of ODC II defects is most likely the reason for the improvement of LIDT.

Bilirubin/morin self-assembled nanoparticle-engulfed collagen/polyvinyl alcohol hydrogel accelerates chronic diabetic wound healing by modulating inflammation and ameliorating oxidative stress

Chronic diabetic wounds pose a serious threat to human health and safety because of their refractory nature and high recurrence rates. The formation of refractory wounds is associated with wound microenvironmental factors such as increased expression of proinflammatory factors and oxidative stress. Bilirubin is a potent endogenous antioxidant, and morin is a naturally active substance that possesses anti-inflammatory and antioxidant effects. Both hold the potential for diabetic wound treatment by intervening in pathological processes. In this study, we developed bilirubin/morin-based carrier-free nanoparticles (BMn) to treat chronic diabetic wounds. In vitro studies showed that BMn could effectively scavenge overproduced reactive oxygen species and suppress elevated inflammation, thereby exerting a protective effect. BMn was then loaded into a collagen/polyvinyl alcohol gel (BMn@G) for an in vivo study to maintain a moist environment for the skin and convenient biomedical applications. BMn@G exhibits excellent mechanical properties, water retention capabilities, and in vivo safety. In type I diabetic mice, BMn@G elevated the expression of the anti-inflammatory factor IL-10 and concurrently diminished the expression of the proinflammatory factor TNF-α in the tissues surrounding the wounds. Furthermore, BMn@G efficiently mediated macrophage polarization from the M1-type to the M2-type, thereby fostering anti-inflammatory effects. Additionally, BMn@G facilitated the conversion of type III collagen fiber bundles to type I collagen fiber bundles, resulting in a more mature collagen fiber structure. This study provides a promising therapeutic alternative for diabetic wound healing.

Novel peptides from sea cucumber intestines hydrolyzed by neutral protease alleviate exercise-induced fatigue via upregulating the glutaminemediated Ca2+ /Calcineurin signaling pathway in mice.

Sea cucumber intestines are considered a valuable resource in the sea cucumber processing industry due to their balanced amino acid composition. Studies have reported that peptides rich in glutamate and branched-chain amino acids have anti-fatigue properties. However, the function of the sea cucumber intestine in reducing exercise-induced fatigue remains unclear. In this study, we enzymatically hydrolyzed low molecular weight peptides from sea cucumber intestines (SCIP) and administered SCIP orally to mice to examine its effects on exercise-induced fatigue using swimming and pole-climbing exhaustion experiments. The results revealed that supplementation with SCIP significantly prolonged the exhaustion time of swimming in mice, decreased blood lactate and urea nitrogen levels, and increased liver and muscle glycogen levels following a weight-loaded swimming test. Immunofluorescence analysis indicated a notable increase the proportion of slow-twitch muscle fiber and a significant decrease the proportion of fast-twitch muscle fiber following SCIP supplementation. Furthermore, SCIP upregulated mRNA expression levels of Ca2+ /Calcineurin upstream and downstream regulators, thereby contributing to the promotion of skeletal muscle fiber type conversion. This study presents the initial evidence establishing SCIP as a potential enhancer of skeletal muscle fatigue resistance, consequently providing a theoretical foundation for the valuable utilization of sea cucumber intestines.

‘Uncertainty audit’ for ecosystem accounting: Satellite-based ecosystem extent is biased without design-based area estimation and accuracy assessment

There are currently no guidelines in the System of Environmental-Economic Accounting Ecosystem Accounting (SEEA EA) for quantifying and disclosing uncertainty. However, without quantifying uncertainty, it is unclear whether or not accounting tables contain biased (erroneous) area estimates which do not reflect real land cover changes. We use Oslo municipality in Norway as a case study to illustrate best practices in quantifying unbiased area estimates using design-based statistical methods. As input for ecosystem extent accounts, we compared a custom Sentinel-2 land cover map with a globally available one called Dynamic World for 2015, 2018 and 2021. The design-based area estimation involved (i) generating a stratified probability sample of locations using the satellite-based maps to define strata, (ii) assigning ecosystem type labels to the samples using photointerpretation according to a response design protocol, and (iii) applying a stratified area estimator to produce 95% confidence intervals around opening, closing and change stocks in the extent accounting table. We found that pixel counting practices, currently adopted by the SEEA EA community, led to biased extent accounts, particularly for ecosystem conversions, with biases averaging 195% of the true change value derived from design-based methods. We found that the uncertainty inherent in state-of-the-art satellite-based maps exceeded the ability to detect real change in extent for some ecosystem types including water and bare/artificial surfaces. In general, uncertainty in extent accounts is higher for ecosystem type conversion classes compared to stable classes, and higher for 3-yr compared to 6-yr accounting periods. Custom, locally calibrated satellite-based maps of ecosystem extent changes were more accurate (81% overall accuracy) than globally available Dynamic World maps (75%). We suggest that rigorous accuracy assessment in SEEA EA will ensure that ecosystem extent (and consequently condition and service) accounts are credible. A standard for auditing uncertainty in ecosystem accounts is needed.