A low-voltage electric field assisted strategy is considered to be effective in improving compost effect of conventional chicken manure composting (CCMC), but it lacks a critical assessment of NH3 mitigation and suitability for complex initial materials. This study firstly constructed an electric field-assisted aerobic co-composting (EFAC) of chicken manure and kitchen waste to evaluate NH3 mitigation and compost maturity. The results showed that the NH3 emissions of EFAC were 48.73% lower than those of CCMC. The proposed mechanisms suggest that the combined effect of reduced acidity and electric field inhibited the activities and functions related to ammoniation and ammonia–nitrogen conversion. The germination index of EFAC was 54.29% higher than that of CCMC, due to the enhancement of compost maturation. This study demonstrates that the electric field-assisted strategy for co-composting has a broad potential to reduce ammonia emissions and enhance the disposal of complex feedstocks.