Emerging contaminants such as antibiotics in wastewater have posed a challenge on conventional biological treatment processes. Algae-bacteria symbiotic mode could improve the performance of biological treatment processes. Self-suspended algae-bacteria symbiotic particles (ABSPs) were prepared with Chlorella vulgaris and Bacillus subtilis using the sol-gel method and hollow glass microspheres in this study. The removal effect of nitrogen and phosphorus as well as the feedback mechanism of ABSPs under tetracycline stress were investigated through three-cycles wastewater treatment experiments. The antioxidant enzyme activity and phycosphere extracellular polymeric substance (EPS) content were identified as well. The results indicated that the removal rates of NH4+-N, TP, COD, and tetracycline in the ABSPs group finally reached 96.18%, 95.44%, 81.36%, and 74.20%, respectively, which were higher than the single algae group apparently. The phycosphere EPS content increased by 20.41% and algae cell structure maintained integrity in ABSPs group as compared with that in single algae group. This study demonstrates that the self-suspended ABSPs can improve contaminants removal performance and alleviate the antioxidant stress response of algae through algal-bacterial reciprocity mechanism.