The single trench fiber (STF) is a promising fiber design for mode area scaling and higher order mode (HOM) suppression. In this Letter, we experimentally demonstrate the strong HOM-suppression in a homemade STF using the spatially and spectrally resolved imaging (S2) technique. This STF has a 20-µm core and its performance is compared to a conventional step-index fiber with almost the same parameter. Results show that the bending loss of the HOM in STF is 8-times larger than conventional fiber at a bend radius of 7 cm. In addition, when severe coupling mismatch is introduced at the input end of the fiber, the STF can keep the fundamental-mode output while the conventional fiber cannot. To the best of our knowledge, this is the first time to experimentally analyze the HOM content in an STF and compare its performance with that of a conventional fiber. Our results indicate the great potential of the STF for filtering the HOM in fiber laser applications.