Conventional Dairy Research Articles

Fermented buttermilk drinks fortified by plant raw materials

The research featured fortified fermented drinks from pasteurized buttermilk with such natural additives as Jerusalem artichoke syrup and beetroot dietary fiber. The optimal symbiotic culture included Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus: it provided rapid fermentation and a creamy, homogeneous structure with delta pH time = 3.5 h. Jerusalem artichoke syrup was added in amounts of 3, 6, and 9%. Its optimal share proved to be 6% by the weight of the finished product. Beet dietary fiber was added in amounts of 2, 4, and 6%, where the optimal amount was 4%. A higher percentage affected the consistency of the finished product but not its clotting or taste. The experimental drinks were produced by the tank method and fermented at 42 ± 2°C until dense clotting and titratable acidity = 72 ± 2°T. The finished product was stored at 4 ± 2°C. The shelf-life was 12 days for the sample with Jerusalem artichoke syrup and 14 days for the drink fortified with beetroot fiber. The physical and chemical indicators showed that the energy value of the fortified fermented buttermilk drinks was on average 45.3% lower compared to conventional fermented dairy drinks. As a result of research, it has been established that the use of plant components, namely Jerusalem artichoke syrup and beet dietary fiber in the production technology of fermented milk drink from buttermilk makes it possible to obtain a finished product with improved consumer properties.

Consumers’ categorizations of dairy products and plant-based milk, yogurt, and cheese alternatives

Plant-based dairy alternatives have many benefits in terms of sustainability, animal welfare, and health, but they can only be successful in the market if consumers perceive them as suitable substitutes for conventional dairy. Consumers' expectations for new products are strongly influenced by the food categories into which they place these products. The present study aims to reveal consumers' categorizations of plant-based dairy products to gain insights into their potential as dairy substitutes. In a free sorting task, 100 participants from the German-speaking part of Switzerland sorted a variety of plant-based and conventional animal-based dairy products into groups, indicating their spontaneous similarity perceptions. Additionally, we assessed the participants' characteristics and attitudes toward plant-based dairy products to test potential differences in categorization strategies among consumer groups. Multidimensional scaling and cluster analysis showed that consumers' mental representations of plant-based dairy and conventional animal-based dairy were clearly separated across a wide range of product types. This pattern was even observed among consumers who ate less meat, had higher exposure to vegan dietary styles, and had less negative attitudes toward dairy alternatives. The results suggest that taxonomic distinctions based on plant or animal origin dominate consumers' perceptions and are likely to hinder the substitution of dairy with plant-based dairy. Nevertheless, they also imply that plant-based products that manage to emphasize shared goals and functional properties akin to conventional dairy products are more likely to form a common goal–derived category in consumers’ minds and thus have better prospects as substitutes.

A registry-based comparative analysis of antibiotic usage reporting for adult cattle on Danish dairy farms

BackgroundAntimicrobial resistance (AMR) is a significant global health concern, necessitating the monitoring of antimicrobial usage (AMU). However, there is a lack of consensus on the standardized collection and reporting of AMU data in the veterinary field. In Denmark, the Danish Cattle Database (DCDB) contains treatment information on animal level, which allows counting of number of treatments carried out, used daily doses (UDD). The Danish VetStat database (VetStat) contains information on veterinary medicinal prescriptions at farm level and uses fixed standard doses of each product to calculate number of daily treatments, animal daily doses (ADD). This study aimed to compare two different numerators, UDD and ADD, used to describe AMU on Danish cattle farms, and estimate their correlation.ResultsRoutinely collected registry data from conventional dairy farms in Denmark for 2019 were used, including a total of 2,197 conventional dairy farms. The data from VetStat and the DCDB were aggregated and analysed, and treatment frequencies (TF) were calculated for both UDD and ADD, adjusting for farm size. Spearman correlation analysis and Bland–Altman plots were employed to assess the relationship and agreement between TF for ADD and UDD, respectively.The results showed a high correlation between TF for ADD and UDD for most prescription groups, i.e., groups used to categorise antibiotics based on target organs. An exception is found for the Udder prescription group, where a systematic underreporting of UDD compared to ADD was observed. This discrepancy may be due to combination treatments, and potential missing or grouped registrations in the DCDB.ConclusionsOur UDD and ADD comparison yields valuable insights on farm-level AMU. We observe strong correlations between UDD and ADD, except for udder treatments, where some farms report only 1/3 UDD compared to ADD, indicating potential underreporting. Further investigations are needed to understand the factors contributing to these patterns and to ensure the accuracy and completeness of recorded information. Standardizing AMU data collection and reporting remains crucial to tackle the global challenge of AMR effectively.

Flexibility exploitation in Net-Zero Energy Factories. A technical and economic study case for dairy systems located in Central and South Europe

The decarbonization of energy systems (electric, thermal, gas, and transportation) requires flexibility to integrate power generated by volatile and renewable energy sources (vRES). Industrial systems can contribute to exploiting the flexibility needed to integrate the electricity generated by vRES into energy systems. Designing industrial systems as Net-Zero Energy Factories (NZEF) offers the possibility of exploiting the flexibility that could be used internally to integrate the power generated by renewable energy sources. This study addresses, on one hand, the presentation of a methodology to identify and quantify the flexibility potential of continuous processes typical in dairy systems. On the other hand, it aims to demonstrate a methodology for designing additional flexibility options to operate dairy systems as NZEF. A model has been developed in which the energetic and material flows of conventional diary systems are simulated. The model allows for the design of conventional dairy systems as NZEF and the evaluation of their potential to exploit flexibility. Two different European climate zones have been investigated: Bari (Italy) and Magdeburg (Germany). This study is relevant for designers and facility managers who are following their decarbonization plans, aiming to operate their industrial facility as Net-Zero Energy. The results of this study show that for dairy systems designed as Net-Zero Energy Factories and located in southern Europe (Bari, Italy), the operational costs are much lower than those operating in central Europe (Magdeburg, Germany). Specifically, integrating an 800 kW PV system reduced operational costs by 16.3 % in Bari, compared to 3.8 % in Magdeburg. Additionally, achieving 97 % vRES integration in Bari requires a 1000 kWh thermal storage capacity, whereas in Magdeburg, a 500 kWh capacity is sufficient. These findings suggest that NZEF designs can be optimized based on regional climatic conditions to maximize cost efficiency and vRES integration. The analysis indicates that sensible heat storage systems should be preferred over electrochemical batteries when designing additional flexibility options, considering both technical and economic criteria.

The LTAR Integrated Common Experiment at Upper Mississippi River Basin-Platteville.

Alternative agronomic practices are needed to address the various climatic, agronomic, edaphic, and water quality related challenges faced by the dairy farmers of the Driftless Area (DA) in the Upper Mississippi River Basin (UMRB). These practices should be innovative in nature, inclusive of regional stakeholders, and sustainable to meet the future food and climate related challenges of Wisconsin agriculture. Here, we outline our Integrated (grazing and cropland) Long-Term Agroecosystem Research Common Experiment at the University of Wisconsin-Platteville Pioneer Farm (UW-P PF) in the UMRB and describe our collaboration in this USDA network. In this field-scale experiment, we are comparing the conventional dairy production system common to this region (i.e., corn-on-corn [Zea mays L.] for 4 years, followed by alfalfa [Medicago sativa L.] for 3 years, with no cover crops) with two alternative dairy production systems-(1) soil health management with no-till, cover crops, and application of a novel manure-based nutrient-rich stable product, and (2) management intensive grazing-and rotational grazing on pastures established with diverse forage-legume mix. Meteorological, edaphic, hydrologic, and agronomic data are collected and analyzed at regular frequencies. Going forward, the experiment will continue as a form of stakeholder-driven adaptive research and receive evaluation on a regular basis to determine whether any changes are required to address the "real-world" challenges faced by the farmers in the Midwest.

Investigating the Dietary Impact on Trans-Vaccenic Acid (Trans-C18:1 n-7) and Other Beneficial Fatty Acids in Breast Milk and Infant Formulas.

Maternal diet plays a significant role in the fatty acid composition of breast milk. Dietary products such as milk and meat are the primary sources of natural TFAs for humans. These peculiar fatty acids hold nutritional significance as they not only lack the detrimental effects of industrially produced trans fats on the endothelium characteristic, but they also exhibit anti-inflammatory properties. The relationship between the presence of eight fatty acids in breast milk (including natural TFAs trans-vaccenic and conjugated linoleic acid) and the maternal diet has been explored, and their abundance has been compared to that of infant formulas. Two cohorts of lactating women, originating from a Spanish region, participated in this study; they adhered to the Southern European Atlantic diet or the Atlantic diet. While the consumption of conventional meat or dairy products does not seem to increase the abundance of TFAs in breast milk, trans-vaccenic and oleic acid are among the most distinctive features of breast milk fat in mothers consuming naturally improved dairy products with an improved fatty acid profile. The most significant differences between natural breastfeeding and formula feeding lie in natural TFAs, since formulas are notably deficient in natural TFAs while being overfortified in alpha-linolenic acid in comparison to breast milk. We suggest an improvement in the formulation of these products through using cow's milk with an optimal fatty acid profile that better mimics the fatty acid composition found in human milk.

Comparative Antimicrobial Resistance and Prevalence of Methicillin Resistance in Coagulase-Positive Staphylococci from Conventional and Organic Dairy Farms in South Korea.

Bovine mastitis (BM) has caused huge economic and financial losses in the dairy industry worldwide, with Staphylococcus aureus as one of its major pathogens. BM treatment still relies on antibiotics and its extensive use often generates methicillin-resistant S. aureus (MRSA) and mupirocin-resistant S. aureus (MuRSA). This study compared the antimicrobial resistance trend in coagulase-positive Stapholococci (CoPS) isolated from BM milk in conventional and organic dairy farms and checked prevalence of MRSA and MuRSA. A total of 163 presumptive Staphylococci were isolated, wherein 11 out of 74 from 4 conventional farms (CF1, CF2, CF3, CF4) and 17 out of 89 from 3 organic farms (OF1, OF2, OF3) exhibited coagulase activity. Multiplex-PCR amplification confirmed at least one coagulase-positive isolate from CF1, CF2, CF3, CF4, and OF1 as S. aureus, denoted by the presence of the nuc gene. Three isolates from CF2 contained the mecA gene, indicating MRSA prevalence, while the MuRSA gene marker, mupA, was not detected in any of the isolates. Antimicrobial testing showed that conventional farm isolates were more resistant to antibiotics, especially ampicillin and tetracycline. This suggests a risk of developing multidrug resistance in dairy farms if antibiotic use is not properly and strictly monitored and regulated.

Investigating the effect of sharing environmental information on consumer responses to conventional and hypothetical ‘precision fermented’ yoghurt

SummaryPrecision fermented dairy (PFD) is a novel technology used to produce milk proteins that can be used to replicate conventional dairy (CD) products. With PFD products likely to be available soon in the United Kingdom, this study aimed to explore consumer acceptance of these products. Specifically, the effect of sharing information related to the process and environmental impact of PFD on overall liking and emotional response for yoghurts labelled as CD and PFD. Overall, all participants (n = 62) were willing to try the yoghurts labelled as PFD, and no significant difference in liking between yoghurts labelled as CD and PFD was found, indicating acceptance and trust. However, sharing information slightly increased liking for PFD yoghurt and evoked more positive emotions (‘understanding’, ‘adventurous’ and ‘enthusiastic’). In contrast, information decreased liking for CD yoghurt and had minimal impact on the emotional response, but made participants feel slightly ‘guilty’. In particular, sharing information led high food‐neophobic and food technology‐neophobic individuals to be more ‘understanding’ towards PFD in comparison to the low‐neophobic groups. Findings suggest emotional responses can provide deeper insights beyond liking, which will benefit the food industry when reviewing consumer attitudes. The results show promise that consumers will accept PFD products when available, provided the novel technology can mimic the sensory properties of CD. Furthermore, when marketing products, sharing information may increase liking for sustainable products, but future studies would benefit from exploring the effect of different types of information on consumer acceptance.

Comparison of Greenhouse Gas Emissions, Nitrogen Intensity, Gross Margin, and Land Use Occupation between Comparable Conventional and Organic Managed Dairy Farms

In this study, 200 Norwegian dairy farms were analyzed over three years to compare greenhouse gas emissions, nitrogen (N) intensity, gross margin, and land use occupation between organically and conventionally managed farms. Conventionally managed farm groups were constructed based on propensity matching, selecting the closest counterparts to organically managed farms (n=15). These groups, each containing 15 farms, were differentiated by an increasing number of matching variables. The first group was matched based on geographical location, milk quota, and milking cow units. In the second match, the proportion of milking cows in the total cattle herd was added, and in the third, the ratio of milk delivered to milk produced and concentrate usage per dairy cow were included. The analysis showed that the conventionally managed farms (n=185) had higher greenhouse gas emissions (1.42 vs 0.98 kg CO2 per 2.78 MJ of edible energy from milk and meat, calculated as GWP100-AR4) and higher N intensity (6.9 vs 5.0 kg N input per kg N output) compared to the organic farms (N=15). When comparing emissions per kg of energy-corrected milk (ECM) delivered, conventional farms also emitted more CO2 (1.07 vs 0.8 kg CO2 per kg ECM). Furthermore, conventionally managed farms showed lower gross margins both in terms of NOK per 2.78 MJ edible energy delivered (5.8 vs 6.5 NOK) and per milking cow unit (30 100 vs 34 400 NOK), and they used less land (2.9 vs 3.6 m² per 2.78 MJ edible energy delivered) compared to organic farms. No differences were observed among the three conventionally managed groups in terms of emissions, N intensity, land use occupation, and gross margin.

Are Organic Farms Less Efficient? The Case of Estonian Dairy Farms

The paper investigates the technical efficiency of conventional and organic dairy farms in Estonia in the period 2006‒2015 using Farm Accountancy Data Network. We analyse self-selection into organic farming using the propensity-score-matching approach and explicitly test the hypothesis that organic and conventional farms apply homogeneous technology. We find that organic farms are less efficient. However, the difference in technical efficiency between organic and conventional farms decreases substantially when the technical efficiency assessment incorporates the use of the appropriate technology. The lack of growth of technical efficiency over time indicates that there might be a lack of knowledge in organic milk production that hinders its development. Since technical efficiency increases with farm size, it is important that organic dairy farms increase their scale.

Investigating the Productivity of Traditional Dairy Farms in Suburbs of Aligarh, India

The productivity index is one of the most efficient indices in economic analysis. This index can compare the existing activity of an operating unit with the desired situation and show the amount of quantitative and qualitative changes made in the production unit. Investigating the productivity of traditional dairy farms is essential because they significantly contribute to India's milk production and are effective in improving the welfare of farmers. This study tries to estimate the productivity of traditional dairy farm units by emphasizing the incomes and expenses of conventional dairy farms (between one and seven heads of dairy farm) using appropriate models. It calculates the highest level of productivity according to the user classes. In this study, the productivity index has been calculated for each user class in 2022-2023 and compared with each other. In addition, the productivity of single-heads, double-heads, and seven-heads has also been calculated. The results of the research showed that the increase in the number of cows leads to a decrease in the cost of maintenance and, as a result, an increase in the productivity index. It was also found that the units of single-head have problems in the field of inseminating cows at the right time due to the lack of timely recognition of cow estrus, that this issue has caused the prolongation of the Reproduction Cycle and the increase of the costs of the dry period of cows, so that the productivity index of single head cattle farms is less than one. Thus, economically, the existence of these dairy farms is not profitable; while in cattle farms with seven dairy cows, this index was calculated as 1.7, which indicates better productivity.

Sensory properties of thickened and protein-enriched plant-based frozen desserts.

The number of consumers following plant-based diets has increased and in turn, the variety of plant-based foods available on the market has also increased. Many plant-based foods aim to mimic the functionality and sensory properties of conventional dairy products; however, they may not be suitable for specific populations. Dysphagia, for example, is a swallowing condition requiring texture-modified foods that meet specific criteria. While many conventional thickened products exist that are safe for individuals with dysphagia, the growing interest in plant-based eating alongside the increasing prevalence of dysphagia prompts a need for research on the use and safety of thickened plant-based alternatives. This study investigated the sensory properties of a thickened protein-enhanced ice cream (dairy and whey) compared to thickened protein-enhanced plant-based frozen desserts (cashew and pea, and coconut and pea). The formulations were evaluated using the International Dysphagia Diet Standardization (IDDSI) Spoon Tilt Test and a sensory trial (n = 104 participants, 47 flexitarians and 57 typical consumers) using static (hedonic scales and check-all-that-apply [CATA]), and dynamic (temporal check-all-that-apply (TCATA)) methods. The dairy and whey sample consistently passed the IDDSI test, while the plant-based samples did not. TCATA identified that the plant-based samples had an increased cohesiveness and adhesiveness, and decreased slipperiness when compared to the dairy and whey sample. The differences in textural properties may explain why the plant-based samples did not pass the IDDSI test. The study identified that although plant-based foods strive to mimic conventional dairy products, they have different textural and flavor properties.

Contribution of plant-based dairy and fish alternatives to iodine nutrition in the Swiss diet: a Swiss Market Survey

PurposeWith dairy products and fish being major sources of iodine in Switzerland, the growing popularity of plant-based alternatives may impact iodine nutrition. This study aimed to assess the iodine content in plant-based dairy and fish alternatives available in the Swiss market and compare them with conventional products.MethodsIn 2022, a market survey was conducted in Zurich, Switzerland, to identify the plant-based dairy and fish alternatives available and assess their iodine content. To evaluate the impact of plant-based alternatives on iodine consumption in Switzerland, we modeled dietary scenarios by substituting the intake of dairy and fish items with plant-based alternatives. In addition, we investigated fortification with calcium, vitamins B2, B12, and D.ResultsOut of 477 identified products, only four milk-alternative products were iodine fortified (median iodine concentration: 22.5 μg/100 ml). The median iodine concentration in unfortified plant-based alternatives was negligible compared to conventional dairy and fish products (milk: 0.21 vs 9.5 μg/100 ml; yogurt 0.36 vs 6.1 μg/100 g; cheese: 0.10 vs 20 μg/100 g; fish 0.50 vs 44 μg/100 g). Three portions of dairy per day as recommended by the Swiss Food Pyramid provide 25% of the RDA for iodine (150 μg/day), whereas substituting those with unfortified alternatives provides only 0.7% of the RDA.ConclusionOnly four out of 477 plant-based alternative products are iodine fortified in the Swiss market. Thus, the risk for consumers to miss out on the ca. 25% of the RDA for iodine by consuming plant-based alternatives is high, placing them at a risk for inadequate iodine intake.

Carcass Yield and Subprimal Cutout Value of Beef, High- and Low-Yielding Beef × Dairy, and Dairy Steers

This study compared carcass yield and cutout value of conventional beef and dairy cattle to high-yielding (HY) and low-yielding (LY) crossbred beef × dairy cattle and identified the contribution of carcass regions to carcass yield andcutout value among beef × dairy crossbreds. Carcasses of conventional beef, beef × dairy crossbred, and dairy cattle were selected according to industry-average slaughter endpoints for their cattle type. Carcasses were fabricated at a commercial processing facility, and weights of carcass components were obtained. Post hoc subsampling was used to segregate HY and LY beef × dairy crossbreds based on subprimal yield. Multiple linear regression was used to assess carcass yield and sub-primal cutout value between the 4 cattle types (n=21 to 26 per cattle type). Beef cattle and HY crossbreds produced 1.59 to 3.04 percentage units greater (P<0.05) subprimal yield than LY crossbreds and dairy cattle. Dairy cattle produced at least 1.16 percentage units more (P<0.05) bone than any other cattle type. Subprimal to bone was not different (P>0.05) between HY crossbreds and beef cattle, and subprimal to fat was lesser (P<0.05) in HY crossbreds than beef cattle. Subprimal cutout value was more than 5 US dollars (USD)/45.4 kg different (P<0.05) between cattle types, which were ranked HY crossbreds > beef cattle > LY crossbreds > dairy cattle. In beef × dairy cattle, subprimal to bone in the round contributed most greatly to an increase (P=0.02), by 3.79 USD/45.4 kg, in subprimal cutout value. Together, these results suggested carcass value of beef × dairy cattle may be maximized when cattle are harvested at a lesser overall fatness than conventional beef cattle and when considerable muscling, especially in the round, is achieved.

An observational study demonstrates human-adapted Staphylococcus aureus strains have a higher frequency of antibiotic resistance compared to cattle-adapted strains isolated from dairy farms making farmstead cheese

BackgroundStaphylococcus aureus is a multi-host zoonotic pathogen causing human and livestock diseases. Dairy farms that make artisan cheese have distinctive concerns for S. aureus control. Antimicrobial-resistant (AMR) S. aureus is a public and animal health concern. There is a need to study the population structure of AMR S. aureus at the human-animal interface and understand the path of zoonotic transmission. This cross-sectional observational study aimed to assess the genetic diversity and AMR patterns of S. aureus isolated from cattle and humans on conventional and organic Vermont dairy farms that produce and sell farmstead cheese.ResultsA convenience sample of 19 dairy farms in Vermont was enrolled, and 160 S. aureus isolates were collected from cow quarter milk (CQM), bulk tank milk (BTM), human-hand and -nasal swabs. After deduplication, 89 isolates were used for the analysis. Sequence types (STs) were determined by multilocus sequence typing and cataloged to the PubMLST database. Nine defined and five novel STs were identified. For BTM and CQM samples, six STs were identified within cow-adapted CC97 and CC151. Two human-adapted STs were isolated from BTM and CQM. Seven human-adapted clonal complexes with eight STs were identified from human samples. One cow-adapted ST was isolated from a human. Antimicrobial susceptibility of the isolates was tested using disc diffusion and broth microdilution methods. Approximately 27% of the isolates were beta-lactam resistant and blaZ gene-positive. S. aureus isolates from human swabs were more likely to carry blaZ compared to isolates from CQM or BTM. S. aureus isolated from cows and humans on the same farm belonged to different STs.ConclusionHumans were more likely to carry beta-lactam-resistant S. aureus compared to cows, and on organic farms only human-adapted blaZ positive STs were isolated from BTM. Moreover, we identified potential spillover events of S. aureus sequence types between host species. The presence of penicillin-resistant-human-adapted S. aureus on both organic and conventional dairy farms highlights a “One Health” concern at the junction of public and animal health requiring further surveillance.

A Business Case for Climate Neutrality in Pasture-Based Dairy Production Systems in Ireland: Evidence from Farm Zero C

Agriculture in Ireland is responsible for producing and exporting healthy, nutritional food pivotal for meeting the Sustainable Development Goals (SDGs) such as global food security, economic development and sustainable communities. However, the agricultural sector, dominated by a large bovine population, faces the challenge of reducing greenhouse gas (GHG) emissions to reach climate neutrality by 2050. The objective of the current study was to model the environmental and economic impact of simultaneously applying farm-level climate change mitigation strategies for a conventional grass-based dairy farm in Ireland. An average farm of 52 ha with a spring-calving herd of 93 was used as a reference scenario to create a business case. Partial budgeting was used to calculate the annual net benefit. A cradle-to-grave life cycle assessment (LCA) was used to model the reduction in GHG emissions, which was expressed as kg of carbon dioxide equivalent per kilogram of fat- and protein-corrected milk (kg CO2-eq/kg FPCM). The baseline for average emissions was 0.960 kg CO2-eq/kg FPCM. An average farm would reduce its annual emissions by 12% to 0.847 kg CO2-eq/kg FPCM in Scenario 1, where climate change mitigation strategies were applied on a minimal scale. For Scenario 2, the emissions are reduced by 36% to 0.614 kg CO2-eq/kg FPCM. In terms of annual savings on cash income, an increase of EUR 6634 and EUR 18,045 in net savings for the farm are realised in Scenarios 1 and 2, respectively. The business case provides evidence that farms can move towards climate neutrality while still remaining economically sustainable.

Comparative Assessment of the Nutritional and Sanogenic Features of Certain Cheese Sorts Originating in Conventional Dairy Farms and in “Mountainous” Quality System Farms

In order to highlight the influence of cattle farming systems on dairy products, assessments were carried out on certain varieties of cheese—marked with the “Mountain product” quality label in comparison with those conventionally produced ones not bearing the quality label. The study was carried out using products obtained from raw milk issued from seven farms and transformed into cheese in four small dairy factories from the mountainous area of Dornelor Basin, Suceava County, Northeastern Romania. The analyzed cheese issued from the “mountain” production system were “Călimani” Schweizer, “Călimani” Cașcaval, “Călimani” smoked Cașcaval, and “Călimani” Telemea—salty brined cheese. Both the “Mountain cheese” and conventional cheese samples produced throughout the same shift were collected and kept under refrigeration conditions until laboratory analysis in order to compare the production systems. The physico-chemical analysis revealed higher amounts of minerals (2.8 to 10.7% Ca; 2.8 to 9.5% P; 12.3% to double the amount of Fe, p < 0.001) and polyunsaturated fatty acids (+5.6 to +13.7%), in mountain cheeses versus the conventionally processed ones. Also, the sanogenic indices had higher values in the “Mountain cheese”, such as the polyunsaturation index (+4.3 to 7.8%) and hypocholesterolic/hypercholesterolic fatty acid ratio (+1.8 to 3.7%), while the atherogenic index and the thrombogenic index had lower values (−1.9 to −4.3%) compared to the conventionally produced cheese, thus revealing healthier properties for consumers. The Enterobacteriaceae family species were identified in “Mountain cheese”, while they were absent from conventionally processed cheese, knowing the raw matter milk is thermally treated at ultra-high temperatures in the latter ones. In the “Mountain cheese”, such microorganisms were found within the safety regulation limits and contributed to providing flavor, taste, color, and specific texture, making it superior in terms of sensorial quality compared to the conventionally produced cheese.

The impact on shelf life of Tulum cheese of modified atmosphere packaging

Tulum cheese is one of the most important conventional fermented dairy products produced in Türkiye. In this study, the effects of modified atmosphere packaging on the microbiological, physicochemical, sensory properties, and shelf life of Tulum cheese were investigated. For this aim, Tulum cheese samples were divided into four groups (B=100% CO2, C =100% N2, D=70% N2+30% CO2, E=75% N2+25% CO2), and the control samples were packaged in the air (A). These samples stored at 4±1°C were analyzed for the microbiological, chemical, and sensory on days 0, 30, 60, 90, 120, 150, 180, 210, and 240. TMAB, LLP, lactic streptococcus, lipolytic microorganisms, proteolytic microorganisms, and yeast-mold counts increased while the counts of coliform group bacteria, Enterobacteriaceae, and Staphylococcus aureus continually decreased in all groups during the storage. During the storage period, the E. coli and sulfate-reducing anaerobic bacteria counts were detected below detectable levels (<1.0 log10 CFU/g) in all groups. Other chemical parameter values increased continuously while pH values continually decreased during the storage in all the groups. It was observed that the total sensory scores of the samples decreased continually during the storage period. However, when the scores of the E group were evaluated within themselves, significant differences were obtained. In conclusion, Tulum cheese samples packaged in MAP showed significant changes in quality, and their shelf life was extended.
 Keywords: Modified atmosphere packaging; Quality characteristics; Sensory evaluation; Shelf-life extension; Tulum cheese

Effect of a dairy farmworker stewardship training program on antimicrobial drug usage in dairy cows

Antimicrobial use (AMU) is critical to preserving animal health and welfare. However, the development of antimicrobial resistance (AMR) represents a public health threat. Although most antimicrobials used on the farm require a veterinarian prescription, farmworkers make daily on-farm treatment decisions. Therefore, farmworker training is vital to promote responsible AMU. This research project aimed to evaluate the effect of farmworker antimicrobial stewardship (AMS) training on the quantity of AMU on dairy farms in Ohio and California. We hypothesized that farms receiving AMS training would have reduced AMU in dairy cattle compared with farms where training wasn't administered. We designed a quasiexperimental study with 18 conventional dairy farms enrolled in Ohio and California. Twelve farms received AMS training, and 6 farms did not. The AMS program included a 12-wk training focused on accurate identification of cows requiring antimicrobial treatment. We quantified AMU by collecting used drug containers, manually counted by researchers during farm visits. Treatment incidence (TI) was calculated using animal daily doses (ADD), and Poisson regression models were used to analyze the TI data. Disease incidence rate (DIR) in lactating cows was 2.2, 1.5, 1.0, 0.4, 0.3, 0.2, and 0.03/1,000 cow-days for mastitis, lameness, metritis, pneumonia, retained placenta, diarrheas, and other diseases (e.g., conjunctivitis, injuries), respectively. The highest TI by antimicrobial class (provided in ADD/1,000 cow-days) was cephalosporin (5.9), penicillin (5.2), tetracyclines (0.4), lincosamides (0.2), and sulfonamides (0.1). Among the trained farms using a within-treatment group analysis, no significant differences were observed in TI pre-intervention (10.9 ADD/1,000 cow-days) compared with TI postintervention (10.3 ADD/1,000 cow-days). Treatment incidence from the training group was 10.8 ADD/1,000 cow-days and although numerically lower, it was not significantly different compared with TI in the control groups at 13.9 ADD/1,000 cow-days (rate ratio = 0.77, CI = 0.25-2.38). Future research on AMS should incorporate farmworker training with social science approaches to overcome barriers and promote on-farm responsible use.

Machine learning approaches to predict and detect early-onset of digital dermatitis in dairy cows using sensor data.

The present study aimed to employ machine learning algorithms based on sensor behavior data for (1) early-onset detection of digital dermatitis (DD) and (2) DD prediction in dairy cows. Our machine learning model, which was based on the Tree-Based Pipeline Optimization Tool (TPOT) automatic machine learning method, for DD detection on day 0 of the appearance of the clinical signs has reached an accuracy of 79% on the test set, while the model for the prediction of DD 2 days prior to the appearance of the first clinical signs, which was a combination of K-means and TPOT, has reached an accuracy of 64%. The proposed machine learning models have the potential to help achieve a real-time automated tool for monitoring and diagnosing DD in lactating dairy cows based on sensor data in conventional dairy barn environments. Our results suggest that alterations in behavioral patterns can be used as inputs in an early warning system for herd management in order to detect variances in the health and wellbeing of individual cows.