Conventional Cigarette Smoking Research Articles

Comparison of Biomarkers of Exposure in a Controlled Study of Smokers Switched from Conventional Cigarettes to Heated Tobacco Products.

The heated tobacco product (HTP) heats rather than burns tobacco to release an aerosol with significantly fewer toxicants than conventional cigarette smoke and has received global attention in recent years. To investigate whether changes in biomarkers of exposure could be detected after switching from conventional cigarettes (CCs) to HTPs, 224 subjects from four cities in China participated in this study. Nine biomarkers containing tobacco-specific nitrosamines (TSNAs), volatile organic compounds (VOCs), and the biomarkers for acrolein and crotonaldehyde were determined by UPLC-MS/MS. The levels of the sum of nine biomarkers in CCs were 5.4 and 5.2 times higher than in an Original-HTP and Menthol-HTP, respectively. Among the nine biomarkers, 3HPMA and 3HMPMA accounted for the highest proportions. Switching from CCs to HTPs is good for both men and women because the changes in each biomarker in urine samples were the same in men and women. Among all the subjects, subjects aged 20-39 years had the greatest reduction in biomarker residues in urine. The findings of the present study provided useful information for the health risk research of HTPs in China.

The relationship between heated cigarette smoking and blood white blood cell count: a population-based cross-sectional study

ObjectivesConventional cigarette smoking increases inflammation and white blood cell (WBC) counts. However, there have been limited studies on the relationship between heated cigarette smoking and WBC counts. This study aimed to examine this relationship using nationally representative population-based health data. Study designThis was a population-based cross-sectional study. MethodsThe Korea National Health and Nutrition Examination Survey database was used to analyze this relationship. Data related to sex, age, body mass index (BMI), WBC count, and smoking history were obtained from the database. The smoking-related questionnaires included smoking status, smoking type (heated or conventional cigarettes), and smoking amount. The summary statistics of the WBC counts were calculated according to sex, smoking status, and smoking type. In addition, the exposure–response relationship between the smoking amount and WBC count was examined by smoking type, controlling for sex, age, and BMI. ResultsIn total, 9747 WBC measurements were used in the analyses. WBC count increased in conventional cigarette smokers, while there was no significant difference in WBC count between heated cigarette smokers and non-smokers. The WBC count showed a positive dose–response relationship with the smoking amount in both conventional and heated cigarette smokers. ConclusionsThe results confirm that conventional cigarette smoking increases WBC counts. Furthermore, the results suggest that heated cigarette smoking does not lead to a significant increase in WBC counts, although it indicates a potential dose–response relationship with WBC count. Further research with larger sample sizes is needed to confirm whether these results reflect true associations.

Vaping E-Cigarette and Smoking Conventional Cigarettes are Associated with Depression Regardless of Gender, Race, and Ethnicity: Analysis of the Health Information National Trends Survey (HINTS) 2022

Background: While previous research has explored the bidirectional relationships between smoking, vaping, and depression, limited attention has been given to examining potential gender-, race-, and ethnic- specific patterns in these associations. Aims: This study aimed to investigate gender, race, and ethnic differences in the associations between smoking conventional and vaping e-cigarettes and depressive symptoms. Methods: Data from the Health Information National Trends Survey (HINTS–2022) survey were analyzed, including a representative sample (probability sample) of US adults. Variables of interest included smoking status, e–cig use, depression (PHQ-4), and demographic characteristics. Binary logistic regression models were employed to assess the association between smoking and e-cig use (current and ever), and depression overall, adjusting for covariates (age, education, marital status, and employment). Statistical Package for the Social Sciences (SPSS) software was used for data analysis, using univariate, bivariate, and multivariable models. We tested potential interactions between race, ethnicity, gender, and depression. Results: The study findings indicated significant associations between smoking, e-cig use, and depression. Individuals who were experiencing depression compared to those non-depressed were more likely to use conventional (p<0.05) and e-cig (p<0.05). However, no significant interactions were found for gender, race, or ethnicity. Conclusions: This study suggests that the association between smoking, e-cig use, and depressive symptoms is not different by race, sex, and gender. These findings contribute to the existing literature, emphasizing the need for universal approaches to promote mental health as a tool to help individuals quit smoking and vaping, regardless of their gender, ethnicity, and race.

E-Cigarette, Conventional Cigarette, and Depression: Role of Race/Ethnicity in the Health Information National Trends Survey (HINTS) 2022

Background: While previous research has established the role of depression as a correlate of tobacco use, limited research has been conducted on potential racial variations in these associations. Aims: This study had two objectives: (1) to investigate the associations between depression and smoking conventional and vaping e-cigarettes, and (2) to explore the role of race in modifying these associations. Methods: Data from the Health Information National Trends Survey (HINTS-2022) survey, which is a national probability sample of US adults, were analyzed. Variables of interest included lifetime and current smoking status, e-cigarette use, depression (PHQ-4), socioeconomic status (SES), and demographic characteristics. Binary logistic regression models were applied to assess the associations between depression as the independent variable and current and ever smoking conventional cigarettes and vaping e-cigarettes as dependent variables, adjusting for covariates (age, gender, education, marital status, and employment). We tested potential interactions between race/ethnicity (non-Hispanic Whites, non-Hispanic Blacks, Hispanic Whites and any other/mixed race/ethnicity) and depression on our outcomes. Results: The study findings indicated significant associations between depression and use of conventional cigarettes and e-cigarettes. Although individuals who were experiencing depression were more likely to use conventional cigarettes (p < 0.05) and e-cigarettes (p < 0.05) compared to those non-depressed, the positive associations between depression and lifetime and current smoking were stronger for Black than White individuals. Conclusions: This study suggests that depression may have a more salient role in smoking cigarettes and e-cigarette use for Black than White adults. It is unclear if Black adults are more likely to smoke if they are depressed, or if Black adults who smoke cigarettes are more likely to be depressed because of higher stigma and lower access to mental health care services.

Dual and Poly Use of Tobacco Products in a Sample of Pregnant Smokers: A Cross-sectional Study

ObjectivesOur aim was to assess the use of dual/poly tobacco in a sample of pregnant women. Design: cross-sectional survey.MethodsTwenty prenatal care units in Botucatu, Sao Paulo, Brazil. We evaluated 127 high-risk pregnant smokers during prenatal care. Those who were 12–38 weeks pregnant and were currently smoking conventional cigarettes. The study enrollment took place between January 2015 and December 2015. The dual/poly prevalence of tobacco products during pregnancy and the characteristics related to smoking in pregnant smokers through a specific questionnaire containing questions related to sociodemographic characteristics, comorbidities, gestational history, smoking history, secondhand smoke exposure, nicotine dependence, motivation stage and use of alternative forms of tobacco.ResultsMean age was 26.9 ± 6.6 years, most had only elementary education and belonged to lower income economic groups. Twenty-five (19.7%) smoked conventional cigarettes only while 102 used conventional and alternative forms of tobacco products. Smoking pack-years was significantly lower in those only smoking conventional cigarettes than in dual/poly users. Proportion of patients with elevated degree of nicotine dependence was higher in conventional cigarettes users. On the other side, alcohol intake was higher in dual/poly smokers when compared to conventional cigarettes group. The alternative forms of smoking were associated with significantly higher occurrences of comorbidities as pulmonary, cardiovascular and cancer.Conclusions for PracticeThe prevalence of alternative forms users of smoking products is high during pregnancy. These data reinforce the importance of a family approach towards smoking in pregnant women and education about the risks of alternative forms of tobacco.

The effect of cigarette smoke exposure on rat’s spermatogenesis: A systematic literature review and meta-analysis

Objective: To conduct a systematic review and meta-analysis of all available published data related to rats about spermatogenesis to provide a comprehensive overview of the effects of cigarette smoke exposure on the parameters of spermatogenesis.Methods: A comprehensive search of electronic databases including PubMed, EBSCO, ProQuest, Science Direct, Taylor & Francis, Springer Link, GARUDA, and Google Scholar, spanning from their inception until January 29th, 2021, was conducted to retrieve relevant studies and full-text articles evaluating the effect of cigarette smoke exposure on the parameters of spermatogenesis in rats. Data were taken from eligible studies. We use SYRCLE’s Risk of Bias tool for this animal studies. The overall size effect was measured, and forest plots and funnel plots were generated using the statistical software JASP.Results: The present study comprised 23 studies involving 527 rats. The findings of this meta-analysis revealed that parameters of spermatogenesis, such as sperm count, morphology, and viability, were substantially impaired in response to conventional cigarette smoke exposure, exhibiting and aggregate correlation coefficient of 0,70 (95% CI:0.61-0.79, p < .001).Conclusion: Exposure to conventional cigarette smoke has a significant negative effect on spermatogenic parameters in rats, particularly in terms of sperm count, morphology, and viability.

Total and bioavailable polycyclic aromatic hydrocarbons in unused and operated heat-not-burn tobacco products and conventional cigarettes

Tobacco product waste poses a global environmental issue, affecting urban and coastal areas alike. The present studies report, for the first time, the total and bioavailable polycyclic aromatic hydrocarbons (PAHs) concentrations in unused and operated heat-not-burn (HnBs) tobacco products. To enable direct comparisons, identical sets of studies were conducted using conventional cigarettes (CCs). Five low-molecular PAHs were determined in HnBs at total concentrations that were of the same order before and after operation (Σ5PAH = 47.37 ± 3.44 ng unit−1 and Σ5PAH = 69.36 ± 5.78 ng unit−1 in unused and used HnBs, respectively). The incomplete combustion of organics during smoking of CCs, yielded substantially higher amounts of PAHs with their sum (Σ10PAHs = 1449 ± 113 ng unit−1) being >20 times larger than those in HnBs. The tobacco and filter were the most contaminated parts in HnBs. In unused CCs, tobacco had the highest PAHs load and after smoking, the spent filter was the most contaminated part, containing ∼80% of the total amount of PAHs. Naphthalene was the most abundant PAH detected in all tobacco products. Despite the high total PAH concentrations found in smoked CCs, the sums of the bioavailable PAH concentrations were of the same order in all tested tobacco products (Σ5PAH = 61.38 ± 1.79 ng unit−1 in unused HnBs, Σ5PAH = 70.87 ± 7.67 ng unit−1 in used HnBs, Σ4PAH = 66.92 ± 5.95 ng unit−1 in unused CCs, and Σ6PAH = 47.94 ± 1.26 ng unit−1 in smoked CCs). This finding was related to smoking affecting PAHs’ leachability from CCs and delaying their desorption from the solid matrix. Adjusting the pH, salt and humic acids content at environmentally relevant values did not affect PAHs leaching at 24 h of soaking. Finally, the leaching behavior of PAHs in natural waters (river water, rainwater, and seawater) was found similar to that in ultrapure water, experimentally verifying the ability of tobacco product waste to leach PAHs into the aquatic environment.

Long-term e-cigarette aerosol exposure causes pulmonary emphysema in adult female and male mice

This study aimed to evaluate long-term exposure to conventional cigarette smoke (CC) and electronic cigarette (EC) aerosol in adult male and female C57BL/6 mice. Forty-eight C57BL/6 mice were used, male (n = 24) and female (n = 24), both were divided into three groups: control, CC and EC. The CC and EC groups were exposed to cigarette smoke or electronic cigarette aerosol, respectively, 3 times a day for 60 consecutive days. Afterwards, they were maintained for 60 days without exposure to cigarettes or electronic cigarette aerosol. Both cigarettes promoted an influx of inflammatory cells to the lung in males and females. All animals exposed to CC and EC showed an increase in lipid peroxidation and protein oxidation. There was an increase of IL-6 in males and females exposed to EC. The IL-13 levels were higher in the females exposed to EC and CC. Both sexes exposed to EC and CC presented tissue damage characterized by septal destruction and increased alveolar spaces compared to control. Our results demonstrated that exposure to CC and EC induced pulmonary emphysema in both sexes, and females seem to be more susceptible to EC.

Comparison of the Smoking Cessation of Heated Tobacco Product Users and Conventional Cigarette Smokers in Korea.

Since the introduction of heated tobacco products (HTPs) in Korea in 2017, their annual sales have increased. Several studies have investigated the perceptions of HTPs and smoking cessation behaviors. For the first time, In 2019, questions focused on HTP use were introduced in the Korea National Health and Nutrition Examination Survey (KNHNES). This study aimed to compare smoking cessation behaviors between HTP users and conventional cigarette smokers using KNHANES data. Data of 947 current adult smokers from the 8th KNHNES (2019) were analyzed. Current smokers were divided into conventional cigarette (CC)-only, HTP-only, and dual-use groups. The general characteristics of the three groups were investigated. Differences in current intention to quit smoking and past attempts to quit smoking among the three groups were analyzed using multivariate logistic regression analysis by IBM SPSS ver. 25.0. HTP-only users demonstrated fewer future smoking cessation plans (adjusted odds ratio [AOR], 0.398; 95% confidence interval [CI], 0.195-0.813; P=0.012) and fewer smoking cessation attempts in the past year (AOR, 0.533; 95% CI, 0.298-0.954; P=0.034) than CC-only smokers. However, there was no significant difference between dual-use (CC+HTP) and CC-only smokers. While dual-use and CC-only smokers showed similar smoking cessation behaviors, HTP-only users had fewer previous attempts to quit smoking and were less likely to be currently ready to quit smoking. These findings can be explained by a decrease in the need to quit smoking due to the convenience of HTP and the perception that HTPs are less harmful than CC.

Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers

RationaleElectronic (e)-cigarettes are popular among youth and cigarette smokers attempting to quit. Studies to date have focused on the utility of e-cigarettes as a smoking cessation tool, but the biological effects are largely unknown.ObjectivesTo identify transcriptomic differences in the blood and sputum of e-cigarette users compared to conventional cigarettes smokers and healthy controls and describe biological pathways affected by these tobacco products.MethodsCross-sectional analysis of whole blood and sputum RNA-sequencing data from 8 smokers, 9 e-cigarette users (e-cigs) and 4 controls. Weighted gene co-network analysis (WGCNA) identified gene module associations. Ingenuity Pathway Analysis (IPA) identified canonical pathways associated with tobacco products.Main resultsIn blood, a three-group comparison showed 16 differentially expressed genes (DEGs); pair-wise comparison showed 7 DEGs between e-cigs and controls, 35 DEGs between smokers and controls, and 13 DEGs between smokers and e-cigs. In sputum, 438 DEGs were in the three-group comparison. In pair-wise comparisons, there were 2 DEGs between e-cigs and controls, 270 DEGs between smokers and controls, and 468 DEGs between smokers and e-cigs. Only 2 genes in the smokers vs. control comparison overlapped between blood and sputum. Most gene modules identified through WGCNA associated with tobacco product exposures also were associated with cotinine and exhaled CO levels. IPA showed more canonical pathways altered by conventional cigarette smoking than by e-cigarette use.ConclusionCigarette smoking and e-cigarette use led to transcriptomic changes in both blood and sputum. However, conventional cigarettes induced much stronger transcriptomic responses in both compartments.

Peri-Implant Inflammation in Waterpipe Users and Cigarette Smokers: An Observational Study

The aim of this study was to compare peri-implant clinical and radiographic status and levels of advanced glycation endproducts (AGEs) in peri-implant sulcular fluid (PISF) in waterpipe users and cigarette smokers. Waterpipe users, cigarette smokers, and never smokers were included. Demographic details were collected using a questionnaire. Characteristics of implants (dimensions, jaw location, depth of placement, insertion torque, and duration in function) were recorded. Peri-implant modified plaque and gingival indices (mPI and mGI), probing depth (PD), and crestal bone loss (CBL) were recorded in all groups. Volume of PISF and levels of AGEs were determined using standard techniques. Sample-size estimation was done on data from a pilot investigation, and correlation between clinicoradiographic and immunoinflammatory parameters was assessed using logistic regression models. Probability values <.05 were considered statistically significant. In all, 25, 25, and 24 cigarette smokers, never smokers, and waterpipe users, respectively, were examined. All participants were male and had comparable mean ages. Cigarette smokers and waterpipe users had a smoking history of 20.2 ± 3.5 years and 18.8 ± 0.6 years, respectively. The mPI (P < .01), CBL (P < .01), PD (P < 0.01), and mGI (P < .01) were significantly higher in cigarette smokers and waterpipe users than never smokers. There was no significant difference in clinicoradiographic status and AGE concentrations in waterpipe users and cigarette smokers. A statistically significant correlation was recorded between AGEs and PD in cigarette smokers (P < .01) and waterpipe users (P < .01). Waterpipe usage is not less hazardous to peri-implant tissue health than conventional cigarette smoking. It is imperative to caution patients with dental implants about the detrimental effects of tobacco products on oral health.

The Effect of Electronic Cigarettes (E-Cigarettes) and Conventional Smoke on the Trachea and Heart of Male White Rats

Electronic cigarettes (e-cigarette) smoker was increasing from 0.3% in 2011 to 2.5% in 2015. E-cigarettes claimed to be safe for smoker, but subsequent research has shown that e-cigarette smoke also contains harmful ingredients beside nicotine, like carbon monoxide (CO). These contents presumed to increase the goblet cells in the trachea and can cause myocardial ischemia in the left ventricle of the heart which leads to necrosis. This study was conducted to determine the effect of e-cigarette and conventional cigarette smoke on tracheal goblet cells and left ventricular necrosis of the heart of male white rats (Rattus norvegicus strain wistar). Experimental study with post-test control only group design. 16 rats were divided into 4 groups: normal group, conventional cigarette group (kretek) with 2.3 mg nicotine, 3 mg nicotine e-cigarette, and 0 mg nicotine e-cigarette. Trachea and heart of rats were prepared using HE-staining and observed with a light microscope with 400x magnification. All data were analyzed using One-way ANOVA, Kruskall Wallis and Post-Hoc tests. Cigarette smoke had a significant effect on tracheal goblet cell (ANOVA p=0.000) and left ventricular necrosis of the heart (Kruskal Wallis p=0.002). Post-Hoc test showed a significant difference in the number of goblet cells and left ventricular necrosis of the heart (p (-- removed HTML --) 0.005). This is due to the toxic materials such as carbon monoxide, propylene glycol, formaldehyde, nitrosamines, and other carcinogenic substances. E-cigarette and conventional cigarettes smoke could affect increasing of tracheal goblet cells and left ventricular necrosis of rat.

An Overview of E-Cigarette Impact on Reproductive Health

Electronic cigarettes (e-cigarettes) are often considered a "safe substitute" for conventional cigarette cessation. The composition of the fluid is not always clearly defined and shows a large variation within brands and manufacturers. More than 80 compounds were detected in liquids and aerosols. E-cigarettes contain nicotine, and the addition of flavorings increases the toxicity of e-cigarette vapour in a significant manner. The heat generated by the e-cigarette leads to the oxidation and decomposition of its components, eventually forming harmful constituents in the inhaled vapour. The effects of these toxicants on male and female reproduction are well established in conventional cigarette smokers. Although toxins were measured at much lower levels in e-cigarette aerosols compared to smoke from a conventional cigarette, there are concerns about their potential impact on male and female reproduction. The information available was mainly obtained from studies conducted in animal models, and investigations in humans are scarce. However, the effects observed in animal models suggest that caution should be taken when vaping and that more research needs to be conducted to identify its potential adverse effects on fertility. The prevalence of e-cigarette usage is alarming, and warnings should be made about the impact of vaping on reproductive health. This document reviews the data regarding the impact of e-cigarette use on male and female reproduction.

Vaping and Health Service Use: A Canadian Health Survey and Health Administrative Data Study.

Rationale: Emerging research suggests that e-cigarette (EC) use may have detrimental health effects, increasing the burden on healthcare systems. Objectives: To determine whether young EC users had increased asthma, asthma attacks, and health services use (HSU). Methods: This cohort study used the linked Canadian Community Health Survey (cycles 2015-16 and 2017-18) and health administrative data (January 2015-March 2018). A propensity score method matched self-reported EC users to up to five control subjects. Matched multivariable logistic and negative binomial regressions were used to calculate odds ratios, rate ratios (RRs), and 95% confidence intervals (CIs) with EC use as the exposure and asthma, asthma attacks, and all-cause HSU as the outcomes. Results: Analyses included 2,700 matched Canadian Community Health Survey participants (15-30 yr), 505 (2.4% of 20,725 participants) EC users matched to 2,195 nonusers. After adjusting for confounders, EC users with asthma had over twofold higher odds of having an asthma attack in the last 12 months (odds ratio, 2.30; 95% CI, 1.29-4.12). Dual EC and conventional tobacco users had a twofold increased all-cause HSU rate compared with nonusers who never smoked tobacco (RR, 2.13; 95% CI, 1.53-2.98). This rate was greater than that for EC users who never smoked tobacco (RR, 1.73; 95% CI, 1.00-3.00) and non-EC users who regularly smoke tobacco (RR, 1.72; 95% CI, 1.29-2.29). Compared with male nonusers, female EC users had the highest increased all-cause HSU (RR, 1.94; 95% CI, 1.39-2.69) over male EC users and female nonusers (RR, 1.13; 95% CI, 0.86-1.48; RR, 1.41; 95% CI, 1.16-1.71, respectively). Conclusions: Current EC use is associated with significantly increased odds of having an asthma attack. Furthermore, concurrent EC use and conventional cigarette smoking are associated with a higher rate of all-cause HSU. The odds of asthma attack and all-cause HSU were highest among women. Thus, EC use may be an epidemiological biomarker for youth and young adults with increased health morbidity.

Exhaled Carbon Monoxide Level and Practices among Tobacco and Nicotine Adult Users in Klang Valley, Malaysia.

Tobacco and nicotine derivatives uses are multiple in nature. These include conventional cigarettes (CCs), heated tobacco products (HTPs), and electronic cigarettes (ECs). This study aims to determine the practices, nicotine dependency profile, association with exhaled carbon monoxide (eCO) level, and pulmonary function (PF) among adult product users and non-smokers. This cross-sectional study involved smokers, nicotine users, and non-smokers from two public health facilities in Kuala Lumpur from December 2021 to April 2022. Data on socio-demography, smoking profile, nicotine dependency level, anthropometry, eCO monitor, and spirometer measurements were recorded. Out of 657 respondents, 52.1% were non-smokers, 48.3% were CC only smokers, poly-users (PUs) (27.3%), EC-only users (20.9%), and HTP-only users (3.5%). EC use was prevalent among the younger aged, tertiary educated, and females; HTP use was prevalent among those of an older age and CC users was common among lower educated males. The highest median eCO (in ppm) seen were as follows: in CC users only (13.00), PUs (7.00), EC users (2.00), HTP users (2.00), and the least was observed among non-smokers (1.00), which is significantly different across the groups (p <0.001). Comparison of practice between the different product users showed significant differences in age of product initiation (p <0.001, youngest in CC users in PUs), duration of product use (p <0.001, longest in exclusive CC users), cost per month (p <0.001, highest in exclusive HTP users) and attempt to quit product (p <0.001, CC use in PUs had the highest attempt to quit), while there is no significant difference in Fagerström score across the groups. Among EC users, 68.2% successfully switched from smoking CCs to ECs. The findings suggest that EC and HTP users are exhaling less CO. The use of these products in a targeted approach may manage nicotine addiction. Switching practice was higher among current EC users (from using CCs), hence emphasizing the need of switching encouragement and total nicotine abstinence later on. Lower eCO levels in the PU group, (as compared to CC-only users) and high quit attempt rate among in CC use in PUs may indicate attempt of PUs in reducing CC use through alternative modalities such as ECs and HTPs.

Burn Injury From Smoking Electronic Cigarettes While on Supplemental Oxygen.

Many reports pertaining to burns from e-cigarette battery explosions exist, as do injuries from smoking conventional cigarettes while on home-oxygen therapy. However, literature regarding burn injuries from e-cigarettes while on oxygen therapy is limited. The heating coil of the device can rise to temperatures that facilitates ignition of oxygen. The purpose of this study is to describe and characterize this novel and under-reported mechanism of injury. This study was a descriptive review of 2013-2016 National Burn Repository (NBR) data, with a query for "oxygen," "O2," "electronic cigarettes," and various permutations/abbreviations/misspellings. Demographics, injury characteristics, and outcomes were reported. Also, a Google search for lay-press articles and a PubMed/Scopus search using similar terms was conducted to find literature. Of approximately 60,000 NBR entries, 8 records of injury while smoking e-cigarettes on oxygen were found. Patients were predominantly male, 63 ± 9 years old, with 3.4% ± 4 TBSA burns, and LOS of 5.8 ± 7 days (an LOS O/E ratio of 1.5). Two patients sustained full-thickness burns (0.5% and 11% TBSA). Three were intubated, with mean of 3.33 ventilator-days. Most injuries occurred at home (88%). All patients survived. A google search revealed five lay-press articles. Only three partially relevant scientific articles were found to only mention the phenomenon, without granular data. Although uncommon, smoking e-cigarettes on supplemental oxygen can result in injury without a lithium-ion battery explosion. Limitations in the database may have resulted in underestimation of injuries. A paucity of relevant literature exists, necessitating more research. Patients who receive home-O2 should be warned about this potential danger.

Underlying patterns of the co-occurrence of tobacco use and mental health among youth.

We aimed to examine how bidirectional relationships between mental health problems and tobacco use are formed over time by types of tobacco use in recent samples of U.S. youth. Data were drawn from Waves 1-4 of the Population Assessment of Tobacco and Health Study (N = 10,082) and analyzed using cross-lagged panel models. A high level of internalizing problems at Wave 1 predicted conventional cigarette smoking (adjusted odds ratio [AOR] = 1.19, 95% CI = 1.06-1.34) and e-cigarette use (AOR = 1.25, 95% CI = 1.09-1.43) at Wave 2, but not vice versa. Both cigarette smoking and e-cigarette use at Wave 2 tended to persist into Wave 3, which, in turn, increased the risk of subsequent internalizing problems in late adolescence or young adulthood (Wave 4). The bidirectional relationship between tobacco use and internalizing problems seems to begin as a procession from internalizing problems to tobacco use, and then from persistent tobacco use to exacerbated internalizing problems over time.

Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants

Electronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48 h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF-α levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women.

Comparison of conventional and electronic cigarette on formaldehyde in blood and alveolus histopathology in vivo

Background: An e-cigarette is a cigarette that does not contain tobacco. The most toxic compounds in conventional cigarette smoke and e-cigarette vapors are carbonyls, such as formaldehyde which induce lung tissue damage. This study aims to compare the effect of exposure and the difference in duration of exposure to conventional cigarettes and electronic cigarettes on blood formaldehyde levels and alveoli histopathological features in Sprague dawley rats. Method: This research is an experimental study with 30 white Sprague dawley rats, male, aged 10-12 weeks, and with body weights of 150-250 grams. Rats will be necropsied after intervention for two weeks and four weeks. Blood samples will be examined for formaldehyde levels, and lung tissue will be assessed for alveolar damage. The results were analyzed by one-way ANOVA statistical for blood formaldehyde levels and Mann Whitney for the degree of histopathological damage to the alveoli. Result: There was no significant difference in blood formaldehyde levels between groups (p&gt;0.05). There was an increasing trend in the intervention group compared to the control and in the fourth-week group compared to the second-week group. There was a significant difference in the degree of alveolar damage in the intervention group compared to the control (p&lt;0.05). There was no significant difference between exposure for 2 and 4 weeks (p&gt;0.05). Between rats given conventional cigarettes and e-cigarettes, there was no significant difference in the degree of alveolar damage at weeks 2 and 4 (p&gt;0.05) Conclusion: Exposure to conventional cigarette smoke or electronic cigarette vapor has a significant effect on alveoli histopathological features

Use of Conventional Cigarette Smoking and E-Cigarette Vaping for Experimental Stroke Studies in Mice.

Cigarette smoking is a major prodromal factor for the onset of many adverse health effects that may occur in the short run and is the leading cause of preventable disease, disability, and death in the United States. Moreover, it is well established that chronic smoking is associated with vascular endothelial dysfunction in a causative and dose-dependent manner primarily related to the release of reactive oxygen species (ROS), nicotine, and the induction of oxidative stress (OS)-driven inflammation. Preclinical studies have also shown that nicotine (the principal e-liquid ingredient used in e-cigarettes) can also cause OS, exacerbating cerebral ischemia and secondary brain injury. Likewise, chronic e-Cig vaping could be prodromal to cerebrovascular impairment and promote cerebrovascular conditions favoring stroke onset and worsening post-ischemic brain injury. Therefore, using mouse models is crucial to understand how xenobiotics such as those released by conventional and/or e-cigs can impact the onset and severity of stroke as well as post-stroke recovery. To appropriately model human-like smoking/vaping behavior in mice, however, the exposure to these xenobiotics must be standardized and undertaken in a controlled environment. This chapter describes a well-validated protocol to reproduce standardized chronic tobacco smoke or e-cigarette vape exposure in mice in the setting of a mouse transient ischemic stroke model.