The temperature in fiber drawing is one of the key factors influencing fiber’s performance. To the temperature measurement and control problem, the four-wire lead configuration of Pt100 with current excitation was adopted, the signal conditioning circuit includes constant current source, instrumentation amplifier and anti-aliasing filter. The computer collected signals from the conditioning circuit and output control signals by data acquisition card PCI-6221M. The software program was based on LabVIEW, the function relationship between resistance and temperature based on international thermometric scale and the digital filter were applied to calculate temperature. The improved PID control algorithm and the PWM technique were used to realize the temperature control. The experiment results show that the deviation is 0.71°C when the average temperature of the furnace center is 170.25 °C, which satisfies the design requirements.