Controlled Drug Delivery Research Articles

A platform of gold nanoparticles coated with silica as controlled drug delivery for application in cancer treatment

A platform of gold nanoparticles coated with silica as controlled drug delivery for application in cancer treatment

Nanotechnology: Current Developments, Applications and Patents for Non-invasive Topical Cosmetics and Dermatological Therapeutic Delivery

: Nanotechnology exhibits the advancement in the field of invention, by improving the potency of the formulation through the delivery of inventive solutions. To resolve several limita-tions related to conventional formulations, nanotechnology applications are increasing in the cosmetics and dermatological area. Cosmetic and dermatological preparations are considered a thriving branch of the body care arena and their usage has increased dramatically through the past years. Scientists are designing innovative delivery mechanisms and emerging innovations that are presently being utilized in the development of cosmetics. Inventive nanocarriers such as nio-somes, liposomes, microemulsions, nanoemulsions, solid-lipid nanoparticles, nanospheres, and nanostructured (lipid) carriers have substituted the use of traditional drug delivery. These innova-tive nanocarriers have the convenience of better skin permeation, sustained and controlled drug delivery, precise location, higher stability, and greater incorporated capability. Since the usage of cosmetics has increased, the usual delivery system has been substituted by modern delivery ap-proaches. The introduction of newer advancements and novel drug delivery systems make cos-metics and cosmeceuticals more popular with increased market share. This review article on nan-otechnology employed in cosmetics and dermatology emphasizes the numerous innovative nanocarriers designed for cosmetic and dermatologic drug delivery, their negative and positive aspects, and their toxicity.

Folic acid functionalized Ag@MOF(Ag) decorated carboxymethyl starch nanoparticles as a new doxorubicin delivery system with inherent antibacterial activity

Considering the benefits of controlled drug delivery in cancer treatment, as well as the importance of biological macromolecules in this area, herein, the pre-synthesized carboxymethyl starch (CMS) was converted to CMS nanoparticles (CMS NPs) in one easy nanoprecipitation way. Thereafter, the Ag@MOF(Ag) was in situ synthesized in the presence of pre-prepared CMS NPs (CMS NPs/Ag@MOF(Ag)). Eventually, the functionalization with folic acid (FA) obtained the CMS NPs/Ag@MOF(Ag)-FA. The success of the accomplished process was approved by doing several techniques, including FT-IR, XRD, EDX, AFM, etc. The SEM analysis showed a combination of rod-like and spherical-like morphology for the fabricated bio-nanocomposite. The generated CMS NPs/Ag@MOF(Ag)-FA with a surface area of 10.595 m2/g displayed a pore size of 13.666 nm and 82.99 % of doxorubicin (DOX) loading efficiency (DOX@CMS NPs/Ag@MOF(Ag)-FA). The 38.46 % and 58.19 % of loaded DOX were released respectively within 240 h at pH 7.4 and pH 5.0, referring to the pH-responsivity of the constructed system. 27.25 % of inhibitory effects on HeLa cells were obtained for the drug-loaded bio-nanocomposite. The CMS NPs/Ag@MOF(Ag)-FA also displayed an inherent antibacterial activity towards two common gram-negative and gram-positive bacteria. All of these results can contribute to developing polysaccharide-based porous systems in controlled cancer therapy.

Enzyme-induced degradation of natural and artificial linear polyanions

Synthetic and natural polymers are widely used for constructing drug delivery systems. Biocompatibility, water solubility and non-toxicity make polymers a convenient matrix for encapsulation, delivery and release of bioactive compounds [1–6]. Coupling of a drug with a biodegraded polymer matrix is a promising way for a controlled drug delivery. Along this line, the degradation of the four polymers in the presence of two enzymes in aqueous solutions was investigated. The following polymers were used: natural polysaccharides, sodium alginate and sodium hyaluronate, artificial (modified) sodium carboxymethylcellulose and synthetic sodium polyacrylate (control); their degradation was caused by the addition of alginate lyase and hyaluronidase. The first enzyme only cleaved the specific alginate substrate and left three other intact. Contrastingly, the second enzyme degraded all three polysaccharides, including artificial carboxymethylcellulose, but did not degrade synthetic polyacrylate. The biodegradation of polymers was accompanied by decreasing the size of polymer particles in solution from 100-200 nm down to 20-30 nm; the latter are capable of removing from the body through the kidneys. The initial polysaccharides showed the negative surface charge in aqueous solution, which changed but retained negative after biodegradation. The initial and biodegraded polysaccharides demonstrated negligible cytotoxicity during long exposure period. The obtained results are valuable for the development of polymer carriers for drug encapsulation and delivery.

Review on Oral Osmotic Tablet

Traditional oral medication delivery methods provide the drug with an immediate release and an efficient concentration at the intended location. Such a dosage regimen could lead to unexpected, ever-changing plasma concentrations. As a result, numerous regulated medication delivery systems are created. Among these, osmotic drug delivery systems (ODDS) and pulsatile drug delivery systems (PDDS) are becoming more and more significant because they improve patient treatment efficacy and compliance by delivering the medicine at precise times based on the course and physiological requirements of the disease. They regulate the drug’s delivery by using the osmotic pressure theory.To a significant extent, the drug’s release is unaffected by GIT physiological variables. Both targeted and systemic medication delivery are possible with these methods. The theoretical idea of drug delivery, various oral osmotic drug delivery system types, factors influencing the drug delivery system, benefits and drawbacks of these delivery systems, fundamental osmotic system components, evaluation parameters, difficulties, and emerging technologies in oral controlled drug delivery are all “highlighted” in this review.

PH-sensitive zinc oxide nanoparticle-controlled sodium alginate/silica hydrogel beads: For controlled curcumin release

This study designed a pH-sensitive carrier regulated by zinc oxide nanoparticles for the delivery of the hydrophobic drug curcumin (Cur). Carboxymethyl cellulose (CMC) was used as a stabilizer and dispersant to synthesize CMC-ZnO nanoparticles via an in situ reaction. These nanoparticles were then mixed with sodium alginate (SA) and immersed in a tetraethyl silicate (TEOS) hydrolysis solution to form sodium alginate/silica-zinc oxide (Ss/CMC-ZnO) composite hydrogel beads. XRD, FTIR, SEM, and UV-vis analyses confirmed the successful synthesis and incorporation of ZnO nanoparticles into the hydrogel beads. Swelling experiments demonstrated that as the concentration of CMC-ZnO increased, when the TEOS molar concentration was 0.205 M, the maximum swelling ratio decreased from 39.83 to 14.03, with the time to reach the maximum swelling ratio extending from 4 to 6 hours. At a TEOS concentration of 0.375 M, the swelling ratio decreased from 26.77 to 12.10, with the swelling time extending from 6 to 7 hours. Hemolysis and cytotoxicity tests confirmed the hydrogel beads' good biocompatibility. Cur was incorporated using a blending method, and in vitro release experiments revealed that the Ss/CMC-ZnO hydrogel beads exhibited excellent pH sensitivity. The addition of CMC-ZnO NPs effectively prolonged the release time of curcumin, indicating potential for applications in controlled drug delivery.

Design and Synthesis of Triazine-Based Hydrogel for Combined Targeted Doxorubicin Delivery and PI3K Inhibition.

Melanoma, an aggressive skin cancer originating from melanocytes, presents substantial challenges due to its high metastatic potential and resistance to conventional therapies. Hydrogels, three-dimensional networks of hydrophilic polymers with high water-retention capacities, offer significant promise for controlled drug delivery applications. In this study, we report the synthesis and characterization of hydrogelators based on the triazine molecular scaffold, which self-assemble into fibrous networks conducive to hydrogel formation. Rheological analysis confirmed their hydrogelation properties, while microscopic techniques including FE-SEM and FEG-TEM provided insights into their morphological networks. The drug delivery capability of these hydrogelators was evaluated using doxorubicin, a widely employed anticancer agent, demonstrating enhanced biocompatibility and reduced side effects compared to free doxorubicin. Additionally, the hydrogelators exhibited inhibitory activity against phosphoinositide 3-kinase (PI3K), a key enzyme frequently mutated in cancer, and also involved in melanoma progression. The dual functionality of this delivery system - controlled drug release and PI3K inhibition - highlights the potential of triazine-based hydrogelators as innovative therapeutic platforms for melanoma treatment.

Poly(Vinyl Alcohol) Drug and PVA-Drug-Surfactant Complex Organogel with Dimethyl Sulfoxide as a Drug Delivery System.

The relevance of active research lies in the need to develop new technologies to improve drug delivery methods for the effective treatment of wound healing. Additionally, the potential application of organogels in other areas of biomedicine, such as creating medical patches with controlled drug delivery, indicates a wide range of possibilities for using this technology. This study focuses on developing controlled drug delivery systems using organogels as carriers for ceftriaxone and ofloxacin. By selecting optimal formulations, organogels were created to immobilize the drugs, facilitating their effective and sustained release. The swelling behavior of the hydrogels was studied, showing a swelling coefficient between 16 and 32%, indicating their ability to absorb liquid relative to their weight. Drug release studies demonstrated that ceftriaxone was released 1.8 times slower than ofloxacin, ensuring a more controlled delivery. Microbiological tests confirmed that the organogels containing ofloxacin exhibited antimicrobial activity against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. However, it was a challenge to estimate activity for the model antibiotic ceftriaxone due to bacterial resistance to it. Organogel poly(vinyl alcohol) (PVA)-DMSO-alginate modifications with surfactant cetylpyridinium bromide led to the formation of a polyelectrolyte complex on the interphase, allowing further enhanced the prolonged release of the drugs. The research identified that the optimal compositions for sustained drug release were organogels with compositions PVA (10%)-PVP (1%) DMSO (50%) and PVA (10%)-DMSO (50%) formulations, illustrating the transparent nature of these organogels making them suitable for ophthalmological application. Various organogels compositions (PVA-DMSO, PVA-poly(vinylpyrrolidone)-DMSO, PVA-DMSO-alginate, PVA-DMSO-PLGA, PVA-DMSO-drug-surfactant) loaded with ceftriaxone, ofloxacin, and surfactant were prepared and characterized, highlighting their potential use in antibiotic patches for wound healing. These organogels illustrate promising results for localized treatment of infections in wounds, cuts, burns, and other skin lesions.

Self-Exfoliated Guanidinium Covalent Organic Nanosheets as High-Capacity Curcumin Carrier.

Drug administration is commonly used to treat chronic wounds but faces challenges such as poor bioavailability, instability, and uncontrollable release. Existing drug delivery platforms are limited by chemical instability, poor functionality, complex synthesis, and toxic by-products. Presently, research efforts are focused on developing novel drug carriers to enhance drug efficacy. Guanidinium Covalent Organic Nanosheets (gCONs) offer promising alternatives due to their high porosity, surface area, loading capacity, and ability to provide controlled, sustained, and target-specific drug delivery. Herein, we successfully synthesized self-exfoliated gCONs using a Schiff base condensation reaction and embedded curcumin (CUR), a polyphenolic pleiotropic drug with antioxidant and anti-inflammatory properties, via the wet impregnation method. The BET porosimeter exhibited the filling of gCON pores with CUR. Morphological investigations revealed the formation of sheet-like structures in gCON. Culturing human dermal fibroblasts (hDFs) on gCON demonstrated cytocompatibility even at a concentration as high as 1000 µg/mL. Drug release studies demonstrated a controlled and sustained release of CUR over an extended period of 5 days, facilitated by the high loading capacity of gCON. Furthermore, the inherent antioxidant and anti-inflammatory properties of CUR were preserved after loading into the gCON, underscoring the potential of CUR-loaded gCON formulation for effective therapeutic applications. Conclusively, this study provides fundamental information relevant to the performance of gCONs as a drug delivery system and the synergistic effect of CUR and CONs addressing issues like drug bioavailability and instability.

The versatility of peptide hydrogels: From self-assembly to drug delivery applications.

Pharmaceuticals often suffer from limitations such as low solubility, low stability, and short half-life. To address these challenges and reduce the need for frequent drug administrations, a more efficient delivery is required. In this context, the development of controlled drug delivery systems, acting as a protective depot for the drug, has expanded significantly over the last decades. Among these, injectable hydrogels have emerged as a promising platform, especially in view of the rise of biologicals as therapeutics. Hydrogels are functional, solid-like biomaterials, composed of cross-linked hydrophilic polymers and high water content. Their physical properties, which closely mimic the extracellular matrix, make them suitable for various biomedical applications. This review discusses the different types of hydrogel systems and their self-assembly process, with an emphasis on peptide-based hydrogels. Due to their structural and functional diversity, biocompatibility, synthetic accessibility, and tunability, peptides are regarded as promising and versatile building blocks. A comprehensive overview of the variety of peptide hydrogels is outlined, with β-sheet forming sequences being highlighted. Key factors to consider when using peptide hydrogels as a controlled drug delivery system are reviewed, along with a discussion of the main drug release mechanisms and the emerging trend towards affinity-based systems to further refine drug release profiles.

Production of Polylactide Nanoparticles Loaded With Isoniazid and Vitamin C: A Promising Candidate for the Treatment of Resistant Forms of Tuberculosis

ABSTRACTNowadays, the method of directed transport of drugs is becoming increasingly important in medicine and pharmacology. It allows for increasing the concentration of delivered substances in a certain place and blocking or severely limiting their accumulation in healthy organs and tissues. One of the promising drug carriers used in the development of controlled drug delivery systems is polylactic acid (PLA). We were interested in using biodegradable polymeric nanoparticles (NPs) loaded with isoniazid (INH) and vitamin C (VC) which have sustained action, targeted delivery, high efficacy, and low toxicity. It will help to expand the use of these simple drugs for the rapid treatment of multidrug‐resistant forms of tuberculosis. Thus, in this work, PLA‐INH‐VC NPs were prepared by double emulsion method where several factors affecting particle size were investigated. TGA/DSC and IR spectroscopy studies identified the presence of molecularly dispersed drugs (INH and VC) in polylactide nanoparticles. VC was found to reduce the degradation of the polymer and thus prolonged the release of the drug from the polymer matrix. PLA‐INH‐VC nanoparticles were analyzed for mycobacterial activity and the nanoparticles were found to inhibit the growth of INH‐resistant mycobacteria.

Chitosan nanoparticle encapsulation improves the effect of donepezil on impaired memory in an amnesia mouse model

ObjectiveAmnesia is one of the most important parts of Alzheimer’s disease (AD). Among the conventional and controlled drug delivery strategies for the pharmacological treatment of AD, nanotechnology-based systems targeting pharmacologically active molecules near their site of action seem to be the most effective. So, this study aimed to investigate the nano-chitosan encapsulated Donepezil on impaired memory in a mouse model of AD.MethodThis study was conducted on 232 male NMRI mice (30–35 mg) and carried out in three phases to identify the optimal ethanol dose for amnesia induction; to evaluate the effect of donepezil alone and donepezil enclosed in chitosan nano-particles after ethanol-induced amnesia. The effect of treatment was evaluated in an inhibitory passive avoidance apparatus. The data was recorded and analyzed in GraphPad Prism software using ANOVA and Tukey post hoc test. p < 0.05 was considered as the first significant level.ResultsIntraperituneally (IP) injection of 0.25, 0.5, 0.75, or 1 mg/kg non-capsulated or chitosan-encapsulated donepezil significantly improved impaired memory compared to that of the controls. However, at doses of 0.25 and 0.5 mg/kg, nano-chitosan-encapsulated donepezil had significantly greater effects than non-encapsulated donepezil (p < 0.0001 and p < 0.05, respectively).ConclusionEncapsulation could reduce the required dose to achieve the desired memory improvement effect. Further studies are required to understand the efficiency of nano-chitosan-encapsulated donepezil administered via different delivery routes and the underlying mechanism of its improved effect on impaired memory.

Synthesis of cefixime loaded PCL/HPMC blend nanoparticles: a controlled release study and in vitro anti-bacterial evaluation

Aim To enhance cefixime’s effectiveness and address drug delivery challenges like concentration at the site, dose, and time, present study investigated the impact of polymer blends on cefixime’s in vitro release profile. Methods Cefixime-loaded nanoparticles were prepared via a modified solvent evaporation method, forming a W/O/W double emulsion. Characterisation included FT-IR, zeta potential, TGA, TEM, and XRD, with in vitro studies and kinetic models used to analyse the release mechanism. Results The PH-4 nanoparticle formulation (80:20 PCL/HPMC, 0.5% PVA) achieved an 81% loading rate, no adverse effects, and a controlled release of 84.66%±2.53 over 30 days. It showed stable physicochemical properties, with in vitro antibacterial tests revealing inhibition zones of 27.4 ± 2.12 mm for E. coli and 17.2 ± 2.23 mm for S. aureus at 12 hours. Conclusion Based on the findings, developed nanoparticulate system containing PCL/HPMC demonstrates its efficacy and safety as a controlled drug delivery method for antibiotics like cefixime.

Thermo-amplifier circuit in probiotic E. coli for stringently temperature-controlled release of a novel antibiotic

Peptide drugs have seen rapid advancement in biopharmaceutical development, with over 80 candidates approved globally. Despite their therapeutic potential, the clinical translation of peptide drugs is hampered by challenges in production yields and stability. Engineered bacterial therapeutics is a unique approach being explored to overcome these issues by using bacteria to produce and deliver therapeutic compounds at the body site of use. A key advantage of this technology is the possibility to control drug delivery within the body in real time using genetic switches. However, the performance of such genetic switches suffers when used to control drugs that require post-translational modifications or are toxic to the host. In this study, these challenges were experienced when attempting to establish a thermal switch for the production of a ribosomally synthesized and post-translationally modified peptide antibiotic, darobactin, in probiotic E. coli. These challenges were overcome by developing a thermo-amplifier circuit that combined the thermal switch with a T7 RNA Polymerase. Due to the orthogonality of the Polymerase, this strategy overcame limitations imposed by the host transcriptional machinery. This circuit enabled production of pathogen-inhibitory levels of darobactin at 40 °C while maintaining leakiness below the detection limit at 37 °C. Furthermore, the thermo-amplifier circuit sustained gene expression beyond the thermal induction duration such that with only 2 h of induction, the bacteria were able to produce pathogen-inhibitory levels of darobactin. This performance was maintained even in physiologically relevant simulated conditions of the intestines that include bile salts and low nutrient levels.Graphical

Modulating drug retention and release via surface anchors using hyperthermia and photothermal effects of Fe3O4-SiO2 core-shell mesoporous nanoparticles

Core-shell nanocarriers (Fe3O4-MSN-TESPA-PEG/DOX) with a core-shell structure were created as possible materials for controlled drug retention and release. With a notable increase in pore volume and surface area, the 3-(Trimethylsilyl)propionic acid (TESPA) anchors on the pore walls of SiO2 nanoparticles (MSN). The doxorubicin (DOX) is controlled and stored by a network of polyethylene glycol (PEG) layers that cover the MSN pore by TESPA anchor as a result of interactions between the layers and the high density of functional groups at the pore mouth. Rather than the Brownian motion contribution, magnetic hysteresis losses account for the magnetic hyperthermia contribution to the heating efficiency of the investigated core-shell configuration. Fe3O4 NPs' magnetism and capacity for photothermal conversion make its core an appealing choice for photothermal treatment. By appropriately adjusting the following variables, the combination of magnetic field and NIR irradiation can control localized heating: i. NIR irradiation density; ii. magnetic field intensity; iii. time of use; and iv. concentration of Fe3O4-MSN-TESPA-PEG/DOX core-shell solution. This combination is ideal for bioapplications and clinical trials. The two-step NIR irradiation procedure or magnetic field intensity control allows for the rapid delivery of roughly seven times the maximum amount of stored doxorubicin in only 5 min. Fe3O4-MSN-TESPA-PEG nanocarriers with their core-shell structure have great potential as therapeutic agents with controllable drug delivery and targetability.

Sodium Alginate/Poly (Acrylicacid) Hydrogel Composite, Potential Carrier for Fibroblast Growth Factor1 (FGF1) Delivery.

Fibroblast growth factor1 is a powerful signaling molecule that plays a critical role in injury repair of diverse tissue by stimulating cell growth and angiogenesis. FGF1 has significant role in the cell fate and regulating inflammation with short half-life and poor in vivo stability. The encapsulation of the growth factor in the hydrogel led to peptide protect from the degradation and/or immune recognition and enable controlled drug delivery over a longer period of time. The aim of this study is to develop and evaluate a hydrogel carrier with adjustable release rate while maintaining bioactivity of FGF1. Here we describe an optimal ratio of sodium alginate and polyacrylic acid without additional cross linker containing optimum amount of FGF1 with the potential of sustained release to be used as a therapeutic agent. The carrier was characterized by FTIR, contact angle and swelling ratio. The activity of FGF1 after release from the hydrogel was confirmed by ELISA and Western blot. Further assessment of genes related to inflammation were evaluated by RTPCR. This hydrogel is able to deliver growth factors by restricting the essential proteins within the matrix to prevent rapid proteolysis and explosive release and is therefore widely applicable.

Poly(ionic liquid)-Based Hydrogel for Emerging Pollutant Removal and Controlled Drug Delivery

Poly(ionic liquid)-Based Hydrogel for Emerging Pollutant Removal and Controlled Drug Delivery

Comparative Study of pH-Responsive and Aggregation Stability of Bosutinib-Loaded Nanogels Comprising Gelatin Methacryloyl, Carboxymethyl Dextran, and Hyaluronic Acid for Controlled Drug Delivery in Colorectal Cancer: An Extensive In Vitro Investigation.

This study investigates the use of pH-responsive nanogels for delivering Bosutinib (BOSU) in colon cancer treatment. Nanogels were formulated using three polymers: hyaluronic acid (HA), carboxymethyl dextran (CMD), and gelatin methacryloyl (GelMA). These nanogels achieved high drug entrapment efficiencies (80-90%) through polymer mixing with BOSU, followed by EDC/NHS cross-linking and sonication. The nanogels were stable, with negative zeta potentials (-20 to -30 mV) and particle sizes between 100 and 200 nm. Fourier-transform infrared analysis confirmed successful methacrylation in GelMA nanogels. Sustained BOSU release at pH 5.0 was observed, resembling tumor environments, compared to slower release at normal pH (7.4). Cytotoxicity tests showed 70-80% cell survival reduction in HCT116 colon cancer cells at higher doses, and GelMA-BOSU nanogels notably reduced cell migration. Antiangiogenic effects were confirmed in a chick chorioallantoic membrane model, highlighting the potential of these nanogels for targeted BOSU delivery in colon cancer therapy.

In silico drug encapsulation using 2-hydroxypropyl-β-CD, tyrosine kinase and tyrosinase inhibition of dinuclear Cu(II) carboxylate complexes

In recent years, copper carboxylate complexes have garnered significant interest for biological applications. This study focuses on 20 Cu(II) carboxylate complexes selected from our previous research. Due to the hydrophobic nature of these complexes, the 2-hydroxypropyl-β-cyclodextrin (2HPβCD) was employed as a carrier to reduce toxicity and increase solubility for controlling drug delivery. Monte Carlo calculations were performed to confirm the interaction between the optimized structures of Cu(II) complexes and 2HPβCD, forming a host-guest system. All the structures were simulated and optimized using DFT-D calculations in Material Studio 2017. The results indicated that a neutral medium is more favorable for the adsorption of these complexes into 2HPβCD. More negative binding energy values suggested strong and energetically favorable adsorption on 2HPβCD. Complexes 4, 5, and 7 exhibited the highest interaction, making them excellent candidates for drug delivery systems. DFT-D calculations were also used to investigate the release of complexes, revealing that complexes 5, 14, and 19 were difficult to release due to their lowest energy. In contrast, complexes 8, 9, and 16 were found to be most efficient to release due to weak non-covalent interactions with 2HPβCD as we can predict from binding energy obtained by DFT-D. No specific trend was observed in the interaction of the complexes with 2HPβCD. Additionally, the effects of these complexes on c-kit tyrosine kinase and Mushroom tyrosinase were studied by molecular docking. The results demonstrated that all the complexes interacted with the active site of respective receptors through hydrophobic interactions. Complexes containing 1,10-phenanthroline and 2,2-bipyrdine were identified as having a strong, spontaneous binding ability with receptors.

Self‐Driving Lab for Solid‐Phase Extraction Process Optimization and Application to Nucleic Acid Purification

Sorptive bioprocesses are the basis for numerous biotechnological applications such as enzyme immobilization, biosensors, controlled drug delivery, water treatment, and molecular purification. Yet due to the complexity of these processes, their optimization is still time, labor, and cost‐intensive. This research presents a flexible self‐driving laboratory (SDL) designed for the accelerated development and optimization of solid‐phase extraction processes. As a use case, the SDL was used to optimize a DNA purification process using silica magnetic beads. Through the integration of robotics, machine learning, and data‐driven experimentation, the SDL demonstrates a highly accelerated process optimization with minimal human intervention. In the multistep purification approach, the system is able to optimize buffer compositions for DNA extraction from complex samples, demonstrating effectiveness in both conventional chaotropic salt‐based methods and innovative chaotropic salt‐free buffers. The study highlights the SDL's capability to autonomously refine process parameters, achieving significant enhancements in yield and purity of the product. This blueprint for future self‐driving optimization of bioprocess parameters showcases the potential of autonomous systems to revolutionize biochemical process development, offering insights into scalable, environmentally sustainable, and cost‐effective solutions.