The objective of this paper is to investigate the dynamic behaviour of post-tensioned concrete flat slabs with different geometries and damping ratios. Four groups of models with different lengths, widths, thicknesses and damping ratios designed according to the AS3600 standard. These were used to determine the influence of each parameter on the vibration serviceability by comparing the control variable method with the reference model. The vibration assessment parameters were used as natural frequency, peak acceleration, and response factor. Both the SCI/CSTR43 standard theoretical calculations method and the Strand7 finite element analysis (FEA) method are used to determine the effect of different geometries and damping ratios on vibration. The feasibility of the Strand7 FEA method for vibration analysis is also assessed by calculating the errors of the two methods. The paper concludes that the Strand7 FEA method is highly accurate and feasible. The span in both directions has a large effect on the natural frequency, and increasing both the slab thickness and the damping ratio are effective methods to improve the vibration serviceability. Based on the research in this paper, recommendations are provided for future vibration design of post-tensioned concrete slabs.