Control Treatment Research Articles

The effect of hydroxy-selenomethionine on the productive and reproductive performance of old broiler breeders.

Selenium (Se) is a rare essential element that plays a vital role in the health and performance of animals. By interfering in the production of antioxidant enzymes such as glutathione peroxidase, thioredoxin reductase and methionine sulfoxide, Se plays a role in reducing the effects of oxidative stress and animal performance. This study aimed to investigate the effect of hydroxy-selenomethionine (OH-SeMet) in the diet of broiler breeder and old broiler breeder roosters on productive performance, reproduction and sperm quality parameters. For this purpose, 260 broiler breeders of the Ross 308 strain were used in a completely randomized design with four treatments and five replications (13 hens and one rooster in each replication). Experimental treatments included: (1) a basal diet without OH-SeMet (T1:control), (2) a broiler breeder diet without OH-SeMet and a rooster diet containing 0.1mg/kg OH-SeMet (T2), (3) broiler breeder diet containing 0.1mg/kg OH-SeMet and rooster diet without OH-SeMet (T3) and (4) broiler breeder and rooster diet contained 0.1mg/kg OH-SeMet (T4). The results showed that T3 and T4 treatments improved egg production, egg weight, egg mass and feed conversion ratio (FCR) compared to the control treatment (p<0.05). The fertility and hatchability percentages of T4 and T2 treatments increased compared to T1 and T3 treatments (p<0.05). The rate of embryonic losses in T1 was higher than in other treatments. However, grade one chickens were higher in T4 than in other treatments (p<0.05). Total motility and viability of sperms were significantly higher in T2 and T4 treatments than in T1 and T3 treatments. The sperm abnormality percentage and sperm MDA concentration decreased in T2 and T4 treatments. Therefore, using OH-SeMet may be a practical approach to help old broiler breeders' production and reproduction performance.

Efecto de la UV-C en la calidad postcosecha de cítricos de la región nororiente del Valle del Cauca, Colombia

Ultraviolet short wave (UV-C) irradiation is an emerging technology employed in postharvest fruits treatment. This method facilitates a reduction in microbial load, triggers an elicitor effect, and prolongs the shelf life of fruits. In this study, UV-C was applied to rangpur red lime, Tahitian lime, and sweet orange cv. 'Sweety,' which are the primary citrus varieties cultivated in the northeastern region of Valle del Cauca, Colombia. The citrus fruits were exposed to UV-C irradiation for 5 min using a lamp positioned 11 cm above the work area. Subsequently, the fruits were stored in plastic baskets for 21 days at 26 °C with a relative humidity of 70%. Positive and negative control treatments were also implemented. The positive treatment involved samples treated with a 150 mg/L sodium hypochlorite solution for 5 min, while the negative treatment comprised samples without any postharvest treatment. The results revealed that UV-C application effectively delayed maturation, prevented granulation, and maintained the physicochemical parameters, morphology, and surface color of citrus fruits. In conclusion, UV-C shows promise as a technology for treating citrus fruits during the postharvest stage, offering a means to mitigate losses, and assuring food availability.

Effects of minimum dissolved oxygen setpoints for aeration in semi-intensive pond production of Pacific white shrimp (Litopenaeus vannamei)

Aeration is considered one of the most critical factors in shrimp farming as it affects the metabolism of shrimp and other living organisms within a pond. Maintenance of proper dissolved oxygen (DO) concentrations is critical, as low DO can cause stress, lower resistance to disease, and inhibit growth. Although the effects of single, short-term hypoxia events have been widely studied, little to no research has examined effects of repeated exposure to hypoxia during multiple diurnal cycles that shrimp may experience in aquaculture ponds. Even though the concept is simple, proper DO management is not simple. It is dependent on numerous factors and often based on anecdotal recommendations. The present trial was conducted to help elucidate the effects of DO management strategies on shrimp performance. The trial examined the effects of three different DO set points for automatic aeration on shrimp production and water quality parameters in earthen ponds. In sixteen earthen ponds (0.1 ha), juvenile Pacific white shrimp (⁓0.030 g) were stocked at a density of 25ind/m2. Minimal DO setpoints that activated automatic aeration in each of the treatments were 2.5, 3.5, and 4.5 mg O2/L, respectively. Shrimp were fed using the AQ1 passive acoustic monitoring system that was set to not feed while DO was below the threshold of a given treatment. Each of the treatments was applied to 5 ponds, except for treatment 1 (2.5 mg/L) which was applied 6 ponds as it was considered of higher risk. Shrimp growth performance and water quality indicators were monitored weekly. At the end of the 79–81-day trial, results showed that different aeration control treatments had no significant effect in terms of growth performance, feed inputs or productivity parameters. The final weight of the shrimp ranged between 33.3 and 33.6 g, with average final yields of 7500–8500 kg per ha. Nonetheless, mean electrical costs differed significantly between treatments, with higher DO setpoints treatments exhibiting a higher aeration cost with no discernible benefit. Water quality parameters showed no significant difference, except for morning and afternoon DO concentrations.

Ross 708 broiler small intestine morphology and immunity improvements in response to in ovo Marek's Disease vaccine administration alone or in conjunction with in ovo and dietary supplemental calcifediol

Investigations were performed to determine the systemic immune and small intestine (SI) morphological responses of Ross 708 broilers to the Marek's Disease vaccine (MDV) administered alone or in conjunction with the in ovo and dietary administration of calcifediol (25OHD3). Live embryonated hatching eggs were assigned at random to 3 in ovo treatments at 18 d of incubation. Pre-specified in ovo treatments were: commercial MDV-alone-injected (50 µL) or commercial MDV containing 1.2 (MDV+25OHD3-1.2) or 2.4 (MDV+25OHD3-2.4) μg of 25OHD3. A non-injected control treatment was also included. For the growing phase, broilers received a commercial diet containing 250 IU of vitamin D3 /kg (control) or a commercial diet supplemented with 2,760 IU of 25OHD3 /kg (Hy-D diet). For determination of serum IgG, nitric oxide, and α-1-acid glycoprotein (AGP) at 14 and 40 d of age (doa), blood was collected from 1 bird per pen (48 total). In the duodenum, jejunum, and ileum of the same bird, villus length (VL), crypt depth (CD), VL to CD ratio (VCR), and villus surface area were also determined. There were no significant dietary x in ovo treatment interactions for any of the variables examined. However, birds fed Hy-D diets had lower serum AGP levels at 14 doa when compared to those fed un-supplemented commercial diets. Additionally, at 40 doa, birds in the MDV+25OHD3-1.2 and MDV+25OHD3-2.4 treatments experienced a decrease in serum AGP in comparison to those belonging to the non-injected and MDV-alone treatment groups. A higher jejunal VCR was observed at 14 and 40 doa in birds that belonged to the MDV+25OHD3-1.2 treatment when compared to those in the non-injected and MDV-alone treatment groups, and dietary Hy-D increased the VL of the duodenum and jejunum in birds at 14 and 40 doa when compared to those fed the commercial diet. In conclusion, both dietary or in ovo administration of 25OHD3 lowered inflammatory reactions and improved the SI morphology of broilers that were in ovo-injected with the MDV.

Potential application of wet-phase modified expandable graphite particles as a novel in-depth profile control agent in carbonate reservoirs

Novel wet-phase modified expandable graphite (WMEG) particles were developed for in-depth profile control in carbonate reservoirs. The harsh environment of carbonate reservoirs (≥ 130 °C, ≥ 22 × 104 mg/L) brings significant challenges for existing profile control agents. WMEG particles were developed to address this problem. WMEG particles were synthesized via intercalation with ultrasound irradiation and chemical oxidation. The critical expansion temperature of WMEG particles is 130 °C, and these particles can effectively expand 3–8 times under high temperature and high salinity water. The core flow experiments show that WMEG particles exhibit a good plugging capacity, profile control capacity, and a better-enhanced oil recovery (EOR) capacity in deep carbonate reservoirs. WMEG particles can be expanded in the formation and form larger particles that bridge the upper and lower end faces of the fracture. Then the high-permeability zones are effectively plugged, and the heterogeneity is improved, resulting in an obvious increase in oil recovery. This research provides a novel insight into future applications of profile control agents for in-depth profile control treatment in carbonate reservoirs.

Effectiveness of thinning in mitigating post-drought legacy effects on Chinese fir in subtropical plantation forests, Southeastern China

Legacy effects following drought are widely detected across worldwide forests, significantly affecting the growth recovery and susceptibility of trees after droughts. Thinning is a common forest management practice used to alter tree growth and growth-climate relationships. Although the effects of thinning on tree response during drought have been investigated, how thinning modulates post-drought legacy effects remains largely unknown. In this study, based on tree-ring data of 140 trees, we examined the effects of thinning on post-drought legacy effects using the quantile mixed effect model for Chinese fir (Cunninghamia lanceolata) in Southeastern China. The tree-ring data were stratified sampling from a thinning experiment applied 10 years ago in 8-year-old plantations and included four thinning intensities (20%, 25%, 33%, and 50% reduction of tree number) and an unthinned control treatment. Drought legacy effects of tree growth positively depended on tree social status with the magnitudes and variations larger in higher status classes. Dominant large trees without thinning management had the greatest drought legacy effects. Although the effects of thinning varied slightly at different quantiles, they all indicated that thinning mitigated the growth legacies after drought and the reduction effect was more pronounced with increasing thinning intensity. Thinning could also reduce post-drought climate sensitivities, but only after moderate thinning (20% and 25% thinning intensity). Heavier thinning (33% and 50% thinning intensity) instead enhanced tree growth responses to climate changes following drought. Thinning intensity needs to be carefully considered to really reap the post-drought benefits of forest thinning management. Our findings suggested that mild thinning offered an alleviation of climate dependency following drought in addition to reducing drought legacy effects on growth, benefiting tree recovery from drought. The results of this study are useful to inform management adaptive strategies for drought-vulnerable plantations under increasingly frequent droughts.

Effect of Cr transition layer on the Al2O3/fe(ceramic/metal) interface microstructure based on binder jetting

Ceramic coating is a well-established method for enhancing the wear and corrosion resistance of metal components. Al2O3 coating has been extensively utilized in industrial applications. However, existing ceramic coating processes often yield coatings that are relatively thin with limited wear and corrosion resistance. Furthermore, achieving efficient and high-quality coatings on complex metal surfaces poses significant challenges. The poor wettability between ceramics and metals further complicates the bonding at their interface. Chromium (Cr), a commonly used alloying element in steel, exhibits excellent interfacial wettability with iron (Fe), making it an ideal transition material for the Al2O3/Fe interface. In this study, complex Al2O3-based ceramic shells were fabricated via Binder Jetting. The Cr coating was coated on the surface of the shell, and then the Fe-based metal liquid was poured into the Al2O3-based ceramic shell to achieve metallurgical bonding at the Al2O3/Fe interface by temperature control treatment. The performance of the Al2O3 ceramic matrix and the microstructure of the Al2O3/Fe interface with the Cr intermediate transition layer were investigated. The results indicate that when the solid loading of the Cr coating is 60 wt%, a uniform, and stable Cr coating can be formed on the surface of the sintered Al2O3-based ceramic. The bending strength of the Al2O3-based ceramic matrix increased from 113.79 MPa to 134.69 MPa. The Cr coating significantly improved the wettability between the Al2O3-based ceramic and the Fe-based metal, reducing the wetting angle from 98.9° to 78.4°. The interface between the Al2O3-based ceramic and Fe-based metal formed FeCr2O4 after infusion, resulting in a well-bonded interface without significant cracks.

The effect of hydroxy-selenomethionine on the productive and reproductive performance of old broiler breeders.

Selenium (Se) is a rare essential element that plays a vital role in the health and performance of animals. By interfering in the production of antioxidant enzymes such as glutathione peroxidase, thioredoxin reductase and methionine sulfoxide, Se plays a role in reducing the effects of oxidative stress and animal performance. This study aimed to investigate the effect of hydroxy-selenomethionine (OH-SeMet) in the diet of broiler breeder and old broiler breeder roosters on productive performance, reproduction and sperm quality parameters. For this purpose, 260 broiler breeders of the Ross 308 strain were used in a completely randomized design with four treatments and five replications (13 hens and one rooster in each replication). Experimental treatments included: (1) a basal diet without OH-SeMet (T1:control), (2) a broiler breeder diet without OH-SeMet and a rooster diet containing 0.1mg/kg OH-SeMet (T2), (3) broiler breeder diet containing 0.1mg/kg OH-SeMet and rooster diet without OH-SeMet (T3) and (4) broiler breeder and rooster diet contained 0.1mg/kg OH-SeMet (T4). The results showed that T3 and T4 treatments improved egg production, egg weight, egg mass and feed conversion ratio (FCR) compared to the control treatment (p<0.05). The fertility and hatchability percentages of T4 and T2 treatments increased compared to T1 and T3 treatments (p<0.05). The rate of embryonic losses in T1 was higher than in other treatments. However, grade one chickens were higher in T4 than in other treatments (p<0.05). Total motility and viability of sperms were significantly higher in T2 and T4 treatments than in T1 and T3 treatments. The sperm abnormality percentage and sperm MDA concentration decreased in T2 and T4 treatments. Therefore, using OH-SeMet may be a practical approach to help old broiler breeders' production and reproduction performance.

Inoculum sources and management of bean scab caused by Elsinoë phaseoli

AbstractBean scab caused by Elsinoë phaseoli is a major challenge to common bean cultivation in Kenya. However, knowledge about its epidemiology and management is limited. This study conducted three experiments in Kakamega, Kenya, evaluating scab inoculum sources and control options. The first experiment evaluated three different inoculum sources over two seasons; bean crop residue caused the highest scab incidence in both the 2021 (62.1%) and 2022 (81.1%) seasons. The second experiment assessed the effect of rotation history on scab over three seasons. Scab incidence consistently surpassed 90% within the first 30 days after planting in half‐yearly rotated fields across all seasons, while the disease was absent after planting in fields with no recent history of legumes. Fields rotated out of legumes consistently had the highest grain yield, averaging 1.5 t ha−1 over the seasons. Additionally, the rotation land treatment displayed significantly more pods per plant during the 2021 (9.7), 2022a (9.8), and 2022b (12.5) seasons. In the third experiment, the impact of five fungicides and four cropping systems on bean scab was investigated over two seasons. Neither fungicide treatments nor cropping systems had an effect on bean scab incidence even when only severe symptoms were considered. Carbendazim had the highest yield (1.9 t ha−1) outperforming the unsprayed control only in the 2022 season while none of the other fungicides outperformed the control treatment in yield parameters in either season. This study emphasizes the challenge of managing bean scab without proper crop rotation and underscores the role of crop residue as a critical inoculum source.

Chemical analysis of the effect of the effect of the use of maggots as a source of protein on the quality of fish feed

Objective: The research aims to analyze the chemical content of making fish food by using maggot as a substitute for fish meal as a protein source to improve the quality of fish feed. Material and method: The research method used was an experimental method with 4 treatments and 3 replications. Treatment A is feed consisting of 45% fish meal, 35% maggot meal, 16% corn meal, and 4% tapioca flour; treatment B is feed consisting of 50% fish meal, 30% maggot meal, 16% corn flour, and 4% tapioca flour; treatment C is feed consisting of 55% fish meal, 25% maggot meal, 16% corn flour, and 4% tapioca flour; and control treatment (k) is feed consisting of 0% fish meal, 50% maggot flour, 16% corn flour, and 4% tapioca flour. Results: The results of the research show that the overall water content of the feed produced is low. Regarding protein content, the highest protein content was in the control treatment. The use of maggot flour in feed has the lowest ash content. Regarding fat content, the higher the dose of maggot flour, the lower the fat content of the fish feed. All treatments tested had a lower carbohydrate content than the control treatment. Conclusion: The use of maggot meal as a substitute for fish meal in this research for making fish feed can support chemical feed quality, which contains water, protein, fat, ash, and carbohydrates according to fish needs.

Effect of refugia plant (Zinnia sp.) population on the presence of stem borer (Scirpophaga innotata Walker) and natural enemies in rice

Abstract. Olsiviana, Yassi A, Melina. 2024. Effect of refugia plant (Zinnia sp.) population on the presence of stem borer (Scirpophaga innotata Walker) and natural enemies in rice. Biodiversitas 25: 2652-2660. This study supports biological control by preserving natural enemies in rice cultivation through habitat modification. The research aimed to analyze the effects of different population levels of refugia (Zinnia sp.) on pest attack rates, the presence and diversity of natural enemies of rice stem borer (Scirpophaga innotata Walker 1863), and rice (Oryza sativa L.) production. The study was designed using a Randomized Group Design (RGD) with four population treatment levels: control (no Zinnia sp.), sparse population (32 polybags of Zinnia sp.), medium population (96 polybags of Zinnia sp.), and dense population (160 polybags of Zinnia sp.). The results showed that the dense population (160 polybags of Zinnia sp.) produced the lowest stem borer infestation rate in the eighth week of observation (1.67%). Pests in all treatments belonged to four orders: Lepidoptera, Hemiptera, Coleoptera, and Diptera, with Hemiptera being the most prevalent. Natural enemies of rice pests belonged to three orders: Coleoptera, Hymenoptera, and Orthoptera. The dense population treatment (160 polybags of Zinnia sp.) yielded the highest values for diversity index (H') at 2.1785, species richness index (R) at 2.1837, and evenness index (E) at 0.9915. This treatment also achieved the highest average rice production at 8.64 tons/ha. Meanwhile, the highest dominance value (C) of 0.2489 was found in the control treatment. Thus, this study supports the idea that increasing the population of Zinnia sp. as a refugia plant can increase the diversity of natural enemies, reduce rice stem borer infestation, and increase rice plant production.

Utilization of Bamboo (Bambusa vulgaris) Shoots in Fresh Pasta Noodle Production

This study aimed to investigate the sensory acceptability of fresh pasta noodles made from bamboo (Bambusa vulgaris) shoots, incorporating different proportions at 0% (control), 25%, 50%, 75%, and 100%. The sensory acceptability of the treatments was evaluated in terms of aroma, texture, appearance, and taste, as well as their overall acceptability. The study also analyzed the significant differences in sensory qualities between the experimental treatments and compared them with the control. A completely randomized design was employed, involving sixty (60) evaluators. The results indicated that the inclusion of bamboo shoot in the noodle mixture adversely affected the sensory quality, with these negative effects intensifying at higher bamboo shoot proportions. In terms of aroma, texture, appearance, and taste, the differences among the experimental treatments, with the exception of the control treatment, were not statistically significant. However, significant differences were observed in these sensory aspects when comparing the control treatment to the experimental treatments. In conclusion, the research suggests that even small quantities of high-nutritional-value bamboo shoot flour can negatively impact product quality. Future studies should focus on improving the sensory quality and shelf life of these noodles, while also exploring methods to reduce production costs.

Efeito da adubação orgânica na produção de ácidos graxos e atividade antidiabética de Portulaca oleracea L.

Portulaca oleracea L., commonly known as purslane, is a plant consumed in Europe, Asia and Africa, with nutraceutical compounds and essential fatty acids whose content exceeds that of vegetables such as spinach and lettuce. On the other hand, organic fertilization improves the physical-chemical characteristics of the soil and thus promotes growth, physiology and secondary metabolism, improving the chemical composition of the plant. In order to evaluate the effect of organic fertilization on fatty acid content and anti-diabetic activity (α-Glucosidase), greenhouse-grown plants were subjected to three treatments: control without additional fertilizers, with compost and vermicompost. The results indicated that vermicompost significantly increased oleic and linoleic acid content. However, palmitic acid content decreased, although other saturated fatty acids such as lauric, myristic and stearic acids did not show variations in the different treatments. Of the polyunsaturated essential fatty acids, linolenic acid (omega-3) increased in the control treatment. On the other hand, the enzyme α-Glucosidase did not show differences in the different fertilization treatments. However, its high percentage of inhibition (60 %) positions it as a species with high antidiabetic potential.

Utilization of Organic Waste as A Natural Detector of Formaldehyde on-Campus Snacks

The aim of this research is to reduce household organic waste through the use of organic waste as a natural detector of formaldehyde content in food snacks. The organic waste used in this study was red garlic skins, dragon fruit skins and purple sweet potato skins. This research is an experimental research. The stages of the research started with the extraction of anthocyanins from the three organic wastes, direct detection of formalin and detection of food ingredients. The formalin concentrations tested were 0.5%, 1%, 2%, 5% and 25%. Detection ability can be seen from the color change between control and formalin treatment. Garlic skin produces a red extract, dragon fruit skin produces an orange extract, and purple sweet potato skin produces a deep red extract. Test results on 20 samples of food snacks around the campus using the three organic waste extracts proved that all samples contained formaldehyde in varying concentrations. The highest 50% formalin content was found in yellow noodles and meatballs. The conclusion is that purple beetle skin, dragonfruit skin, and red garlic skin can be used as natural detectors for formalin in campus snacks.

Effect of Sappan Wood Ethanol Extract in CRP Level and Phagocytic Index Between Group of Mice Infected with S. aureus and E. coli

S.aureusand E.coli are pathogenic bacterial that cause many infectious disease in the world. Immunomodulator is needed to prepare the immune system to be able against the infection. Some parameters usually used to assess the immunomodulatory activity such as C-Reactive Protein (CRP) and phagocytic index. This study aims to investigate the difference effect of sappan1wood extract in CRP level and phagocytic index between group mice infected with S. aureus and E. coli. Two treatment groups of mice were prepared for S. aureus and E. coli test. Each group subjected to 7 treatments i.e. (1) Normal mice1(CMC-Na 1 %- Merck® 217277), 1(2) Negative Control (bacterial infection + CMC-Na 1 %),11(3) Positive Control (bacterial infection + Imboost force®1treatment, PT SOHO Industri Pharmasi), (4) Bacterial infection and EESW treatment 25 mg/kg BW, (5) 50 mg/kg BW, (6) 100 mg/kg BW, and (7) 200 mg/kg BW. Mice blood was taken to detect the CRP and phagocytic index after treatment. The T test showed that there was a significant difference between CRP levels (p0.05) and phagocytic index (p0.05) of S. aureus and E. coli group. EESW 200 mg/kg BW reduced CRP level to 11 mg/dL (S. aureus) and 6 mg/dL in (E. coli). EESW 200 mg/kg BW increased phagocytosis to 1.54 folds (S. aureus) and 4.62 folds (E. coli). Sappan wood ethanol extract effect to CRP level and phagocytic index1in mice group infected with E. coli is better than S. aureus1infection group.

Performance of bajra napier hybrid under varying levels and frequency of MgSO4 nutrition in humid tropics

A field experiment was conducted at AICRP on Forage Crops and Utilization, College of Agriculture, Vellayani, to assess the influence of varying levels and frequency of MgSO4 application on the performance of bajra napier hybrid during the period October 2020-September 2021. The experiment was laid out in factorial randomized block design (FRBD) with nine treatments and a control replicated thrice. The treatment combinations included three levels of magnesium [m1: 80 kg ha-1, m2: 100 kg ha-1 and m3: 120 kg ha-1] and three frequencies of application [f1: Split application once in 6 months, f2: Split application once in 4 months and f3: Split application once in 3 months] and a control treatment. Considering the performance of bajra napier hybrid, varying levels and frequencies of MgSO4 application had a significant influence on the growth, yield, biochemical and quality attributes as well as the soil nutrient status after the conduct of the experiment and economics of cultivation. Interaction effect of the treatment combinations revealed higher tiller count (27.07), leaf: stem ratio (1.99), green fodder yield (GFY) (1891.30 q ha-1), dry fodder yield (DFY) (473.00 q ha-1), crude protein content (10.06 %) and benefit-cost ratio (2.11) with split application of 100 kg ha-1 MgSO4 once in 6 months.

Alternative Treatments for Emotional Experiencing and Processing in People with Migraine or Tension-Type Headache: A Scoping Review.

This narrative review aims to summarize the use of alternative treatments (e.g., relaxation training, meditation, written intervention) for emotional expression, processing, control, or management in patients with migraine and tension-type headaches, which the previous literature has shown to be related to migraine pain perception and headache symptoms. Online databases including PubMed, Scopus, Web of Science, PsycINFO, and Medline were searched to identify studies published between 2000 and 2023. A descriptive synthesis of the included studies was conducted. We included seven articles after screening 1.173 records. A total of 610 patients with a diagnosis of migraine or tension-type headache, and an average age of 19-45.5 years (68-90.4% females) were recruited in the selected studies. Overall, the results show that alternative approaches to headache treatment contribute to the management, reduction, or control of negative emotions and at the same time have a positive impact on pain perception and headache symptoms. However, in some cases, the effects are more promising than others, depending on the peculiarities or limitations of each approach considered. This review provides useful insights from a methodological point of view for future studies on the management or control of negative emotions in patients with migraine and tension-type headache.

Analysis of Fatty Acid and Protein Content in Chicken Heart Fed Maggot (Hermentia illucens) and Microalgae Aurantiochytrium sp

Chicken is the most consumed food commodity in Indonesia. Chicken also contains protein which is needed by the body to maintain body strength. However, due to the Covid-19 pandemic, chicken prices have soared due to the increasingly expensive feed prices along with the increase in demand for chicken in the market. This study aims to determine the effectiveness of feeding Maggot Hermetia illucens and Microalgae Aurantiocyhtrium sp. as a source of protein and fatty acids in Joper chickens (Gallus domesticus). Using a completely randomized design (CRD) consisting of 6 treatments and 4 repetitions. Treatment P0 (100% Bran, Corn, Pur feed), P1 (Maggot 30%, microalgae Aurantiochytrium sp. 1.5%, Bran, Corn, Pur 68.5%), P2 (Maggot 35% and microalgae Aurantiochytrium sp. 2%, Bran, Maize, Pur 63%), P3 (Maggot 40% and microalgae Aurantiochytrium sp. 2.5%, Bran, Maize, Pur 57.5%), P4 (Maggot 45% and microalgae Aurantiochytrium sp. 3%, Bran, Maize, Pur 52%), P5 (Maggot 50% and microalgae Aurantiochytrium sp. 3.5%). Parameters observed were final body weight and daily weight gain rate of joper chickens. The results of this study showed that the provision of P5 treatment gave the best results in increasing protein and omega-3 levels with results of 18.19% and 4.53% respectively compared to the protein and omega-3 content of the control treatment which only amounted to 16.62% and 1.17% respectively. This study provides results with treatment P5 which has a major effect on the growth of joper chicken (Gallus domesticus). The results of this study can be an option for chicken feed because it is proven to increase protein and omega-3 content in chickens.

Response of Arugula to Integrated Use of Biological, Inorganic, and Organic Fertilization.

This study evaluated the effects of solely and integrated application of inorganic (NPK), commercial organic (NC), and biological (MIX, mixed strains Ensifer meliloti and Azotobacter chroococcum) fertilizers on the chemical characteristics of arugula biomass and its yield, as well as changes in soil microbiological parameters after the experiment in relation to the control treatment (Ø). The experiment was performed in semi-controlled greenhouse conditions, in pots, from the 4th decade of March to the 2nd decade of September, in 2023, at three cutting times/swaths, during one agricultural season, with Vertisol soil. For soil characterization, the following parameters were analysed: granulometric composition using sieving and sedimentation procedure; soil acidity-potentiometrically; SOM-soil organic matter by Kotzmann method; total N using CNS analyser; available P-spectrophotometrically; available K-flame photometrically; total number of microorganisms on an agarized soil extract medium; fungi on a solid Czapek agar; actinomycetes on a solid Krasiljnikov agar with saccharose; Azotobacter spp. on a liquid Fyodorov medium with mannitol; ammonifiers on a liquid medium with asparagine; and dehydrogenase activity-spectrophotometrically. For plant characterization, the following parameters were determined: N and C, both on CNS analyser; P on spectrophotometer; K on flame photometer; air-dried yield biomass. A stimulative effect on all microbiological parameters was found in the treatment with integrated use of organic and biological fertilizer, except for fungi, which grew better in the treatments with separate inorganic and organic fertilizers. Generally, the stimulative impact on plant chemical parameters manifested in combined inorganic and biological, organic and biological, and inorganic and organic fertilization treatments, and was inhibited in treatment without fertilization, in all three swaths, which could also be stated for the plant yield. Positive influence of all fertilization treatments on chemical parameters was observed for the second swath in relation to the first and the third. The total yield in the NPK+MIX treatment was 121%, and in the NC+MIX treatment, it was 87% higher compared to the control (Ø). In general, integrated use of inorganic and biological, organic and biological, and inorganic and organic fertilizers, respectively, could be proposed as an optimal fertilization treatment in arugula cultivation.

Analysis of Fatty Acid and Protein Content in Chicken Heart Fed Maggot (Hermentia illucens) and Microalgae Aurantiochytrium sp

Chicken is the most consumed food commodity in Indonesia. Chicken also contains protein which is needed by the body to maintain body strength. However, due to the Covid-19 pandemic, chicken prices have soared due to the increasingly expensive feed prices along with the increase in demand for chicken in the market. This study aims to determine the effectiveness of feeding Maggot Hermetia illucens and Microalgae Aurantiocyhtrium sp. as a source of protein and fatty acids in Joper chickens (Gallus domesticus). Using a completely randomized design (CRD) consisting of 6 treatments and 4 repetitions. Treatment P0 (100% Bran, Corn, Pur feed), P1 (Maggot 30%, microalgae Aurantiochytrium sp. 1.5%, Bran, Corn, Pur 68.5%), P2 (Maggot 35% and microalgae Aurantiochytrium sp. 2%, Bran, Maize, Pur 63%), P3 (Maggot 40% and microalgae Aurantiochytrium sp. 2.5%, Bran, Maize, Pur 57.5%), P4 (Maggot 45% and microalgae Aurantiochytrium sp. 3%, Bran, Maize, Pur 52%), P5 (Maggot 50% and microalgae Aurantiochytrium sp. 3.5%). Parameters observed were final body weight and daily weight gain rate of joper chickens. The results of this study showed that the provision of P5 treatment gave the best results in increasing protein and omega-3 levels with results of 18.19% and 4.53% respectively compared to the protein and omega-3 content of the control treatment which only amounted to 16.62% and 1.17% respectively. This study provides results with treatment P5 which has a major effect on the growth of joper chicken (Gallus domesticus). The results of this study can be an option for chicken feed because it is proven to increase protein and omega-3 content in chickens.