Rabies Control Research Articles

Investigation of Rabies virus in wild mammals of the atlantic forest in Rio de Janeiro, Brazil.

With the successful control of rabies transmitted by dogs in Brazil, wild animals have played a relevant epidemiological role in the transmission of rabies virus (RABV). Bats, non-human primates and wild canines are the main wild animals that transmit RABV in the country. It is worth highlighting the possibility of synanthropic action of these species, when they become adapted to urban areas, causing infections in domestic animals and eventually in humans. This work aimed to evaluate the circulation of RABV in the Pedra Branca Forest, an Atlantic Forest area, located in the state of Rio de Janeiro, Southeast Brazil. Saliva and blood samples were obtained from 60 individuals of eight species of bats, captured with mist nets, and 13 individuals of callitrichid primates, captured with tomahawk traps. Saliva samples were subjected to Reverse Transcription Polymerase Chain Reaction (RT-PCR), targeting the RABV N gene, with all samples being negative. Blood samples of all animals were submitted to the Rapid Fluorescent Focus Inhibition Test (RFFIT) to detect neutralizing antibodies (Ab) for RABV. Six bat samples (8%) were seropositive for RABV with antibody titers greater than or equal to 0.1 IU/mL. The detection of Ab but not viral RNA indicates exposure rather than current RABV transmission in the analyzed populations. The results presented here reinforce the importance of serological studies in wildlife to access RABV circulation in a region.

Rabies in rural northeast India: A case report emphasising the urgency of the One Health approach

Dog-mediated rabies is endemic in India. The country records the highest mortality due to dog-bite-related rabies despite the availability of interventions to prevent deaths. We present a case study of the death of a 59-year-old man in a suburban town of Northeast India after a dog bite from an owned pup. Through this case study, we investigate various omissions and commissions in communities and health professionals that make rabies rampant in India. The circumstances surrounding the death were investigated by interviewing the wife, relatives, neighbour, the hospital/nursing home where the bite case was reported, the district Rapid Response Team (RRT), and the Veterinary and Animal Health Department Officer and through the information recorded in the disease outbreak report. While the biting animal was not vaccinated and had no restriction over its movement imposed by the owners, the response of the hospital staff and public authorities was delayed and inadequate. A poignant reminder of the complexities surrounding dog-mediated rabies in India, this case study calls for a holistic protocol to address dog bites through ensuring the One Health approach encompassing education, provision of post-exposure prophylaxis (PEP) and canine rabies vaccines for dogs, promotion of responsible dog ownership, and intersectoral collaboration. Moreover, strengthening communication channels through effective data exchange and encouraging synergy among healthcare, veterinary, and public health sectors is indispensable to maximize the impact of rabies prevention and control interventions.

Phase sensitive slow light in semiconductor quantum dots

This article presents a theoretical investigation of phase-controlled propagation of slow light under an electromagnetically induced transparency window in a multilayered one-dimensional photonic crystal with semiconductor quantum dot nanostructures as defects. To achieve the electromagnetically induced transparency window, a setup utilizes a faint probe laser pulse alongside two powerful control laser beams arranged in a closed-loop cascade configuration. In this arrangement, a significant Kerr nonlinearity, approximately on the scale of ∼10−13m2/V2, is discerned within the quantum dot nanostructures at a probe wavelength of 1.55 μm. It is observed that both the amplitude and the phase relationship of the control Rabi frequencies play crucial roles in governing the dynamics of the group velocity of the probe field in the quantum dot nanostructures. Under the regime of electromagnetically induced transparency, the group velocity of the probe field is significantly reduced, approximately by a factor of 103 times as compared to the light speed in free space. These findings hold promise for potential applications in nonlinear optics.

Ethical considerations in companion animal care: Examining the consequences of rabies in an unvaccinated Boerboel dog and implications for animal welfare policies

AbstractThis case investigates and documents the occurrence of rabies in an unvaccinated 4‐month‐old Boerboel dog in Ebonyi State, Nigeria, emphasising the ethical considerations and implications for existing animal welfare policies. Despite Nigeria enacting the Animal Diseases (Control) Act, 2022, cases of rabies persist, contributing to thousands of human rabies cases annually. The legal framework highlights the ethical responsibilities of pet owners, mandating annual rabies vaccinations and holding owners accountable for their pets' actions. This case report details the dog's behavioural changes, subsequent euthanasia and laboratory confirmation of rabies. Investigations reveal gaps in vaccination compliance with challenges in community cooperation for free vaccination initiatives. The case highlights the global significance of rabies control, highlighting the ethical responsibilities of pet owners, emphasises the consequences of rabies, and advocates for strengthened policies and improved compliance to effectively combat rabies and protect both animal and human welfare in Nigeria.

Comparing Intradermal (ID) Rabies Vaccination with Conventional IM Regimen on Humoral Response of New Zealand White Rabbits for the Production of Animal-Derived Polyclonal Antibodies.

In developing countries, it is imperative to implement cost-effective strategies for animal humoral response development in the production of antiserum. This study compared the effect of immunization regimens on the humoral immune response of New Zealand White (NZW) rabbits (N = 24) using cell culture rabies vaccine (CCRV) through intradermal (ID) and traditional intramuscular (IM) routes. The rabbits were divided into three experimental groups: (a) IPC-R2 with a two-site one-week regimen; (b) TRC-R3 with a two-site twenty-eight-day regimen; and (c) Alternate-R4 with a four-site one-week regimen. These regimens were then compared to the standard IM schedule of five doses of rabies vaccine administered at days 0, 3, 7, 14, and 28 in control group R-1. The results were evaluated at days 14 and 35 postvaccination using rabies-specific Platelia II™ ELISA kit method. The results showed a better response to the ID regimen than the IM route regarding immunogenicity and volume consumption of the vaccine. The three selected ID regimes showed significantly higher mean titer values than the control IM regimen group R-1 (p < 0.001). The study aims to explore simple immunization strategies to enhance the RV-specific antibody titers for immunization donor animals. This method would produce polyclonal antibodies and strengthen local production of polyclonal antibodies in Pakistan to deal with vaccine and rabies immunoglobulin (RIG) shortage, thus providing effective postexposure prophylaxis (PEP) for better control of rabies in developing countries.

PROGRESSIVE CONTROL AND MANAGEMENT STRATEGIES IN THE ELIMINATION OF RABIES AMONG THE STRAY DOG (CANIS FAMILIARIS) POPULATION IN PAKISTAN

Stray dogs are integrally linked with rabies transmission, and a sizeable unmanaged dog population hinders rabies control program planners. Dog population management (DPM) is one of the best strategies to control rabies transmission, improve health management, and lower the population size of stray dogs. The threat of rabies is very serious due to free-roaming dogs. Foxes, skunks, groundhogs, raccoons, and house bats are rabies vector species and can carry rabies virus without showing any symptoms. Rabies is a life-threatening disease that affects thousands of losses worldwide every year. World Rabies Day is an international awareness campaign coordinated by the Global Alliance for Rabies Control (GARC). In Pakistan, there are reportedly 3 million stray dogs. More than one million dog bite cases are reported annually throughout Pakistan. About 600 dog bite cases are reported daily in the Sindh province, Pakistan. Due to an uncontrolled number of stray dogs, many serious problems arise related to public health, socio-economic, and political issues. The Animal Act of 1890 provides a specific defense against cruelty and establishes its importance in Pakistani culture. Developing long-term and supportable strategies is imperative to overcome the stray animal population worldwide. To overcome the rabies issue in Pakistan, the government should implement projects on breeding control of stray dogs through spaying and neutering surgeries. The government should also provide proper shelters for dogs and rabies vaccines. There is a need to be aware of basic preventive measures such as disinfectants and rabies vaccination. Public awareness, rabies vaccination, and dog sterilization at the national level are crucial factors in preventing rabies and managing the dog population in Pakistan.

Effective biological control of chickpea rabies (Ascochyta rabiei) through systemic phytochemical defenses activation by Trichoderma roots colonization: From strain characterization to seed coating

Chickpea (Cicer arietinum) is a legume of great economic and agricultural importance worldwide, whose crop is severely affected by rust or Ascochyta blight, caused by the fungus Ascochyta rabiei. The fungal genus Trichoderma includes several species widely characterized as effective biological control agents against crop pathogens. First, this work characterized several species of the genus Trichoderma as potential biological control agents of A. rabiei directly (in vitro confrontation) or indirectly in chickpea plants (activation of systemic resistance), selecting T. harzianum EN1 as the most efficient strain. Subsequently, different materials were tested as coatings to apply the T. harzianum conidia on chickpea seeds, determining that gum arabic at 1 % concentration was the one that most promoted the germination of conidia and seeds. The third phase of the study was based on the application of the coating and T. harzianum conidia on chickpea seeds and to study plant survival after infection with the pathogen A. rabiei, characterizing root colonization by Trichoderma and systemic hormonal and metabolic changes related to the induction of systemic defenses. The treatment of chickpea seeds with gum arabic and T. harzianum conidia was found to increase Trichoderma-root colonization and to improve plant survival. The induction of systemic ethylene- and melatonin-mediated resistance, which led to the accumulation of nicotinic acid in plant tissues, was considered the cause of such a protection. Therefore, T. harzianum applied as seed coating with gum arabic could be a good biological control strategy against A. rabiei on chickpea, due to the induction of systemic resistance.

Dog ecology and rabies control including canine vaccination coverage: Impacts from a survey in Madagascar.

Rabies virus (RABV; species Lyssavirus rabies) is causing one of the oldest zoonotic diseases known to mankind, leading to fatal encephalomyelitis in animals and humans. Despite the existence of safe and effective vaccines to prevent the disease, an estimated 99% of human rabies deaths worldwide are caused by dog-mediated rabies with children at the highest risk of infection. Rabies has been endemic in Madagascar for over a century, yet there has been little research evaluating local knowledge and practices impacting on the rabies control and prevention. Thus, this study was undertaken to better understand the dog ecology including canine vaccine coverage and to assess knowledge and practices of dog owners and veterinarians. A cross-sectional study was conducted among 123 dog-owning households in thirteen fokontanys in Mahajanga from July 4 to September 13, 2016. Single and multi-member dog-owning households in the study area on the day of the interview were eligible for inclusion and purposively selected with the support of a local guide. The survey included a household questionnaire capturing information on the dog's demographics, husbandry practices, knowledge and practices towards rabies and its control measures; the dog ecology questionnaire collected dog characteristics, vaccination status and husbandry practices. All households that reported a dog bite incident, were invited to participate in a dog bite questionnaire. In addition, direct observations of roaming dogs were conducted to assess dog population demographics and to document behavioural characteristics. Two veterinarians were purposively selected and took part in an interview during the survey period, providing information on rabies control activities, including dog-care practices in the area. Descriptive and inferential data analyses were performed using Epi Info version 7.1.5.0 (CDC Atlanta, USA). We recorded a total of 400 dogs, of which 338 (84.5%) were owned amongst 123 households. More than half (67.8%) of owned dogs were between 1 to 5 years old and 95.6% were kept for guarding purposes. 45% of the surveyed dogs had free access to roam outside the premises. The majority (85.4%) of dog owners were knowledgeable that a dog bite could potentially transmit RABV to humans. 19 dog bites were reported and of these 73.6% were caused by the owner's or a neighbour's dog. In 6 of the 19 cases, children between 7 and 15 years of age were the victims. Dog vaccination coverage against rabies was 34% among owned dogs. Of the participants aware of a veterinarian, the majority (55/82) indicated that they accessed veterinarian services at irregular intervals. The main obstacles to vaccinations cited by dog owners were limited financial resources and difficulty accessing veterinary care. This study contributes to enhanced understanding of the dog ecology including canine vaccine coverage as well as knowledge and practices of dog owners in Madagascar. Most dogs in the study area were accessible for preventive vaccination through their owners, however only one third of the investigated canine population was vaccinated against rabies. Concerted national efforts towards rabies prevention and control should aim to address financial challenges and access to veterinary services.

An assessment of the operationality and factors influencing the effectiveness of rabies surveillance in Gombe State, Nigeria.

Rabies remains a burden in Africa, disproportionately affecting the most vulnerable despite the availability of effective vaccines. Nigeria, the most populous African country, needs rapid disease control actions and commitments to achieve the goal of eliminating dog-mediated rabies by 2030. Surveillance is an essential element of effective disease control strategies. This study examined the current state of operationality of the rabies surveillance system for early case detection and management in Gombe state, Nigeria, through a One Health lens. It further examined the barriers impeding the effectiveness of the surveillance based on the perception of surveillance workers. Qualitative and quantitative methods were used to assess the structure of the system and its functioning. Data on dog bite and rabid cases obtained from the veterinary services in Gombe state were analysed descriptively. A total of 13 key informants were interviewed using a semi-structured interview guide. Qualitative data were analysed using thematic analysis to explore in depth the factors that influenced the operationality of the system. A total of 157 potential human exposures to rabies were identified in this study, out of which two people reportedly died at the health facility after showing symptoms highly suggestive of rabies. In terms of rabies surveillance and control, cross-sectoral collaboration was found between the human health and veterinary sectors for risk assessment of potential rabies exposures and its management. Some identified factors affecting the operations of the surveillance were inadequate funding, lack of infrastructure, lack of feedback from higher authorities and insufficient knowledge of rabies prevention and management. To improve the capacity for case detection and management within the state, the appropriate authorities may focus on increasing awareness about the disease to the populace to increase the number of cases identified by the system, employ more workers and strengthen the surveillance capability of existing workers.

Evaluation of LN34 Pan-Lyssavirus RT-qPCR assay for rabies diagnosis in Brazil

Rabies, a fatal zoonotic viral disease affecting mammals, including humans, remains a significant global health concern, particularly in low-income countries. The disease, primarily transmitted through infected animal saliva, prompts urgent diagnosis for timely post-exposure prophylaxis (PEP). The gold standard diagnostic test, direct fluorescent antibody test (dFAT), while sensitive, suffers from limitations such as subjective interpretation and high costs. As a confirmatory technique, the LN34 Pan-Lyssavirus RT-qPCR assay has emerged as a promising tool for universal Lyssavirus detection. This study evaluated its performance using 130 rabies virus isolates representing eleven Brazilian variants and 303 clinical samples from surveillance operations. The LN34 assay demonstrated 100% sensitivity and 98% specificity compared to dFAT. Additionally, it detected all samples, including those missed by dFAT, indicating superior sensitivity. The assay's specificity was confirmed through Sanger nucleotide sequencing, with only a minimal false-positive rate. Comparative analysis revealed higher accuracy and concordance with dFAT than traditional rabies tissue culture infection tests (RTCIT). False-negative RTCIT results were attributed to low viral load or suboptimal sampling. These findings underscore the LN34 assay's utility as a confirmatory technique, enhancing rabies surveillance and control in Brazil. Its widespread adoption could significantly improve diagnostic sensitivity, crucial for effective PEP and public health interventions.

Genotypic analysis of RTS,S/AS01E malaria vaccine efficacy against parasite infection as a function of dosage regimen and baseline malaria infection status in children aged 5–17 months in Ghana and Kenya: a longitudinal phase 2b randomised controlled trial

The first licensed malaria vaccine, RTS,S/AS01E, confers moderate protection against symptomatic disease. Because many malaria infections are asymptomatic, we conducted a large-scale longitudinal parasite genotyping study of samples from a clinical trial exploring how vaccine dosing regimen affects vaccine efficacy. Between Sept 28, 2017, and Sept 25, 2018, 1500 children aged 5-17 months were randomly assigned (1:1:1:1:1) to receive four different RTS,S/AS01E regimens or a rabies control vaccine in a phase 2b open-label clinical trial in Ghana and Kenya. Participants in the four RTS,S groups received two full doses at month 0 and month 1 and either full doses at month 2 and month 20 (group R012-20); full doses at month 2, month 14, month 26, and month 38 (group R012-14); fractional doses at month 2, month 14, month 26, and month 38 (group Fx012-14; early fourth dose); or fractional doses at month 7, month 20, and month 32 (group Fx017-20; delayed third dose). We evaluated the time to the first new genotypically detected infection and the total number of new infections during two follow-up periods (12 months and 20 months) in more than 36 000 dried blood spot specimens from 1500 participants. To study vaccine effects on time to the first new infection, we defined vaccine efficacy as one minus the hazard ratio (HR; RTS,S vs control) of the first new infection. We performed a post-hoc analysis of vaccine efficacy based on malaria infection status at first vaccination and force of infection by month 2. This trial (MAL-095) is registered with ClinicalTrials.gov, NCT03281291. We observed significant and similar vaccine efficacy (25-43%; 95% CI union 9-53) against first new infection for all four RTS,S/AS01E regimens across both follow-up periods (12 months and 20 months). Each RTS,S/AS01E regimen significantly reduced the mean number of new infections in the 20-month follow-up period by 1·1-1·6 infections (95% CI union 0·6-2·1). Vaccine efficacy against first new infection was significantly higher in participants who were infected with malaria (68%; 95% CI 50-80) than in those who were uninfected (37%; 23-48) at the first vaccination (p=0·0053). All tested dosing regimens blocked some infections to a similar degree. Improved vaccine efficacy in participants infected during vaccination could suggest new strategies for highly efficacious malaria vaccine development and implementation. GlaxoSmithKline Biologicals SA, PATH, Bill & Melinda Gates Foundation, and the German Federal Ministry of Education and Research.

Human cat borne rabies as the new epidemiology of the disease in the Andes mountains.

Rabies virus (RABV) is the etiologic agent of rabies, a fatal brain disease in mammals. Rabies circulation has historically involved the dog has the main source of human rabies worldwide. Nevertheless, in Colombia, cats (Felis catus) have become a relevant species in the epidemiology of rabies. To characterize rabies cases in humans in Colombia in the last three decades in the context of the epidemiology of the aggressor animal. We conducted a retrospective longitudinal epidemiological study of human rabies caused by cats' aggression, collecting primary and secondary information. Variables considered included the demography of the patient, symptoms, information about the aggressor animal as the source of infection and the viral variant identified. We found that the distribution of rabies incidence over the years has been constant in Colombia. Nevertheless, between 2003 and 2012 a peak of cases occurred in rural Colombia where cats were the most frequent aggressor animal reported. Most cats involved in aggression were unvaccinated against rabies. Cat's clinical signs at the time of the report of the human cases included hypersalivation and changes in behaviour. Human patients were mostly children and female and the exposure primarily corresponded to bite and puncture lacerations in hands. The RABV lineage detected in most cases corresponded to variant 3, linked to the common vampire bat (Desmodus rotundus). The geographical presentation of cat borne RABV in humans occurred along the Andes mountains, epidemiologically known as the rabies red Andean corridor. By finding cats as the primary source of rabies spillover transmission in Colombia, this report highlights the importance of revising national rabies control and prevention protocol in countries in the Andes region. Our results demonstrate that rabies vaccination for outdoor cats needs to prioritize to reduce the number of rabies-related human deaths.

Probable rabies case report in a Kangayam bull in Udumalpet, India: Insights into rabies surveillance and control strategies in resource-limited settings

Probable rabies case report in a Kangayam bull in Udumalpet, India: Insights into rabies surveillance and control strategies in resource-limited settings

Unveiling Urban Canines: Leveraging Single-sight and Sight–resight Survey for Street Dog Population Estimation and Enhanced Rabies Surveillance in Metropolitan City of Bruhat Bengaluru Mahanagara Palike, Karnataka, India

Aim: The aim of the study is to estimate Bengaluru's street dog population and evaluate the effectiveness of interventions for population control and public health, particularly in rabies control. It also aims to develop a blueprint for responsible urban management that prioritizes compassion and safety for both humans and street dogs. Study Design and Methodology: The study utilizes the Bruhat Bengaluru Mahanagara Palike (BBMP) Street Dog Survey to estimate the street dog population in Bengaluru. It employs Single-Sight and Sight–Resight methods with Lincoln–Petersen's Formula and Chapman’s Correction for estimation. The population is categorized into zones to provide nuanced insights, guiding targeted interventions based on gender, age composition, and neutering status. During the initial six days, a single-sight survey was conducted by a pair of surveyors traveling on a 2-wheeler down every road in an allocated zone, photographing and recording details of dogs observed. Subsequently, on the following six days, all dogs sighted during the initial survey period were documented, regardless of whether they were previously recorded. Results: The study estimates Bengaluru's street dog population at approximately 279335. It indicates a significant 10% reduction in the street dog population since 2019 which had estimated it at 309898, suggesting effective interventions. There is also a commendable 20% increase in neutering rates from an estimated 51.16% in 2019 to 71.85% in the present study, highlighting the importance of ongoing efforts in population control and public health, particularly in rabies control. Conclusion: The reduction in the street dog population is deemed pivotal for curtailing disease transmission and ensuring public safety. The study positions itself to develop a blueprint for responsible urban management, prioritizing compassion and safety for both humans and street dogs. By exemplifying the effectiveness of evidence-driven policies and collective action, the aim is to pave the way for sustainable urban management practices that foster harmonious coexistence between communities and street dogs. Through continued collaboration and informed decision-making, the study suggests striving towards creating a safer and more compassionate environment for all inhabitants of Bengaluru.

IJCM_293A: A Reflective study about knowledge assessment and feedback of medical officers involved in the training programme for management of Animal bite as per NRCP guidelines &amp; Snakebite in Tertiary HealthCare Centre in Central India

Background: A training program for Management of Animal-bite as per NRCP (National Rabies Control Program) guidelines &amp; Snakebite of Medical Officer was conducted in the Tertiary Healthcare Centre. Evaluation of such training has been rarely reported. Objective: The objective of this study was to reflect on the quality of this training program regarding knowledge assessment and feedback. Methodology: A pre-test and post-test cross-sectional assessment of knowledge of medical officers working at health facilities of Bhopal district of Madhya Pradesh government, who attended one-day training program at AIIMS Bhopal in 2023, were conducted through the same questionnaire having 18 single-answer multiple choice questions. Anonymous feedback was also taken through a semi-structured questionnaire. Data were analyzed using R statistical software. The Chi-square test and Wilcoxon signed-rank test was used to compare the pre-and post-test proportions of correct responses and scores, respectively. Results: Out of a total of 54 participants, 64.8% were males. The median (IQR) age was 41 (31,52) years. The majority of the participants (68.5%) had the qualification of MBBS, 14.8% had PG degree, and the rest, 16.6%, had BAMS degree. Median (IQR) pre and post-test scores were9.0 (9.0, 12.0) and 15 (12, 16.0) respectively. All participants agreed that the training was very useful for the patient care and gave positive feedback related to the management of training. Conclusion: This training was very useful in terms of improvement of knowledge is concerned. More Tertiary Healthcare institutions should take the initiative to conduct such training programs for capacity building of Medical Officers working at community level.

Lessons for the world: Rabies resurgence in rabies free Timor-Leste and the urgent actions needed for prevention measures

Rabies, a fatal viral illness transmitted from animals to humans, remains a globally significant public health concern. Timor-Leste, previously considered rabies-free, reported its first fatal human rabies case in March 2024, signaling a resurgence of the disease. The case involved a 19-year-old female bitten by a dog in December 2023, highlighting gaps in post-exposure prophylaxis (PEP) administration. Subsequent investigations revealed 29 suspected rabies cases, prompting urgent public health responses including dog vaccinations and healthcare worker training. Despite challenges such as limited vaccine availability, comprehensive strategies advocated by the World Health Organization (WHO) offer hope for rabies control. This event underscores the critical need for proactive measures including vaccination programs, public education, surveillance enhancement, and regional cooperation to prevent rabies emergence and safeguard public health in Timor-Leste and beyond.

Empowering rural Maharashtra: A holistic approach to rabies control and one health perspectiv

Rabies, a grave threat in rural areas with limited healthcare access, poses significant global concern due to its near 100% fatality rate in humans and animals. The shift to intradermal vaccines highlights cost-effectiveness. Maharashtra, India’s one of largest states, faces unique challenges in rabies prevention and management, despite efforts, owing to poor awareness and limited healthcare access. In India, Maharashtra stands out due to challenges in rabies prevention and management despite ongoing efforts. This study proposes transformative strategies grounded in community engagement, education and vaccination campaigns to reduce rabies incidence. Emphasizing community empowerment, it advocates for integrating traditional knowledge with modern interventions. Globally, 330,000 people face rabies risk annually, with India accounting for 36% of cases. Limited vaccine access perpetuates transmission risks, necessitating collaborative efforts to enhance awareness and intervention, particularly in rural Maharashtra. The study emphasizes removing stray dogs and implementing widespread vaccination as key prevention strategies. The success of Goa’s One Health program exemplifies human rabies elimination feasibility and cost-effectiveness, aiming to eradicate canine-mediated human rabies deaths by 2030. Targeted interventions can effectively reduce rabies incidence, safeguarding both human and animal populations. By targeting these key areas, communities can effectively reduce the incidence of rabies and protect both human and animal population.

Mixed methods to evaluate knowledge, attitudes and practices (KAP) towards rabies in central and remote communities of Moramanga district, Madagascar.

Control of dog-mediated rabies relies on raising awareness, access to post-exposure prophylaxis (PEP) and mass dog vaccination. To assess rabies awareness in Moramanga district, Madagascar, where rabies is endemic, two complementary quantitative and qualitative approaches were carried out in 2018. In the quantitative approach, a standardized questionnaire was administered to 334 randomized participants living in 170 households located less than 5 km from the anti-rabies treatment center (ARTC) located in Moramanga city (thereafter called the central area), and in 164 households located more than 15 km away from the ARTC in two rural communes (thereafter called the remote area). Logistic regression models were fitted to identify factors influencing knowledge and practice scores. The qualitative approach consisted in semi-structured interviews conducted with 28 bite victims who had consulted the ARTC, three owners of biting dogs, three ARTC staff and two local authorities. Overall, 15.6% (52/334) of households owned at least one dog. The dog-to-human ratio was 1:17.6. The central area had a significantly higher dog bite incidence (0.53 per 100 person-years, 95% CI: 0.31-0.85) compared to the remote area (0.22 per 100 person-years, 95% CI: 0.09-0.43) (p = 0.03). The care pathway following a bite depended on wound severity, how the dog was perceived and its owner's willingness to cover costs. Rabies vaccination coverage in dogs in the remote area was extremely low (2.4%). Respondents knew that vaccination prevented animal rabies but owners considered that their own dogs were harmless and cited access and cost of vaccine as main barriers. Most respondents were not aware of the existence of the ARTC (85.3%), did not know the importance of timely access to PEP (92.2%) or that biting dogs should be isolated (89.5%) and monitored. Good knowledge scores were significantly associated with having a higher socio-economic status (OR = 2.08, CI = 1.33-3.26) and living in central area (OR = 1.91, CI = 1.22-3.00). Good practice scores were significantly associated with living in central area (OR = 4.78, CI = 2.98-7.77) and being aware of the ARTC's existence (OR = 2.29, CI = 1.14-4.80). In Madagascar, knowledge on rabies was disparate with important gaps on PEP and animal management. Awareness campaigns should inform communities (i) on the importance of seeking PEP as soon as possible after an exposure, whatever the severity of the wound and the type of biting dog who caused it, and (ii) on the existence and location of ARTCs where free-of-charge PEP is available. They should also encourage owners to isolate and monitor the health of biting dogs. Above all, awareness and dog vaccination campaigns should be designed so as to reach the more vulnerable remote rural populations as knowledge, good practices and vaccination coverage were lower in these areas. They should also target households with a lower socio-economic status. If awareness campaigns are likely to succeed in improving access to ARTCs in Madagascar, their impact on prompting dog owners to vaccinate their own dogs seems more uncertain given the financial and access barriers. Therefore, to reach the 70% dog vaccination coverage goal targeted in rabies elimination programs, awareness campaigns must be combined with free-of-charge mass dog vaccination.

Investigating Rabies Prevention and Control: A Study of Knowledge, Attitudes, and Practices in Jimma Town, South-Western Ethiopia

Introduction: Ethiopia has the second highest number of human rabies deaths among African countries. However, the lack of public awareness regarding rabies prevention and control is a major obstacle in combating this disease. To address this issue, the current study was conducted in Jimma Town, southwestern Ethiopia, to assess the knowledge, attitude, and practice (KAP) of households regarding rabies and its associated factors. Materials and methods: The current community-based cross-sectional study was carried out, involving a total of 421 participants. The KAP of the respondents was evaluated using principal component analysis and ranked accordingly. Results: Out of the 421 study participants, 53% were female and 63.4% were male. Among the participants, 62.3% demonstrated an adequate level of knowledge, 51% exhibited a positive attitude, and 43% practiced effective prevention and control measures against rabies. On the other hand, younger and male participants were found to have positive attitudes towards rabies. The study revealed that individuals with lower levels of education, specifically those who had not completed college as well as the unemployed and those without dogs had poor knowledge attitudes, and practices about rabies. Conclusion: These findings highlight the need to improve the understanding and awareness of rabies within the community.

Spatial clusters, temporal behavior, and risk factors analysis of rabies in livestock in Ecuador

Rabies, a globally distributed and highly lethal zoonotic neglected tropical disease, has a significant impact in South America. In Ecuador, animal rabies cases are primarily linked to livestock, and hematophagous bats play a crucial role in disease transmission. This study aims to identify temporal trends, spatial patterns, and risk factors for animal rabies in Ecuador between 2014 and 2019. Epidemiological survey reports from the official Animal Rabies Surveillance Program of the Phyto and Zoosanitary Regulation and Control Agency of Ecuador (AGROCALIDAD) were used. The Animal Rabies Surveillance Program from AGROCALIDAD consists of an official passive surveillance program that receives reports from farmers or individuals (both trained or untrained) who have observed animals with neurological clinical signs and lesions compatible with bat bites, or who have seen or captured bats on their farms or houses. Once this report is made, AGROCALIDAD personnel is sent for field inspection, having to confirm the suspicion of rabies based on farm conditions and compatibility of signs. AGROCALIDAD personnel collect samples from all suspicious animals, which are further processed and analyzed using the Direct Fluorescent Antibody (DFA) test for rabies confirmatory diagnosis. In this case, study data comprised 846 bovine farms (with intra-farm sample sizes ranging from 1 to 16 samples) located in different ecoregions of Ecuador; out of these, 397 (46.93%) farms tested positive for animal rabies, revealing six statistically significant spatial clusters. Among these clusters, three high-risk areas were identified in the southeast of Ecuador. Seasonality was confirmed by the Ljung-Box test for both the number of cases (p < 0.001) and the positivity rate (p < 0.001). The Pacific Coastal lowlands and Sierra regions showed a lower risk of positivity compared to Amazonia (OR = 0.529; 95% CI = 0.318 – 0.883; p = 0.015 and OR = 0.633; 95% CI = 0.410 – 0.977; p = 0.039, respectively). The breeding of non-bovine animal species demonstrated a lower risk of positivity to animal rabies when compared to bovine (OR = 0.145; 95% CI = 0.062 – 0.339; p < 0.001). Similarly, older animals exhibited a lower risk (OR = 0.974; 95% CI = 0.967 – 0.981; p < 0.001). Rainfall during the rainy season was also found to decrease the risk of positivity to animal rabies (OR = 0.996; 95% CI = 0.995 – 0.998; p < 0.001). This study underscores the significance of strengthening the national surveillance program for the prevention and control of animal rabies in Ecuador and other countries facing similar epidemiological, social, and geographical circumstances.