Control Measurements Research Articles

Increased Afferent Nerve Firing Is Correlated With the Detection of Bladder Wall Micromotion in a Perfused Ex-Vivo Porcine Model.

Observable autonomous rhythmic changes in intravesical pressure, termed bladder wall micromotion, is a phenomenon that has been linked to urinary urgency, the key symptom in overactive bladder (OAB). However, the mechanism through which micromotion drives urinary urgency is poorly understood. In addition, micromotion is inherently difficult to study in human urodynamics due to challenges distinguishing it from normal cyclic physiologic processes such as pulse rate, breathing, rectal contractions, and ureteral jetting. Therefore, the goal of this study was to create a reproducible model of micromotion using an ex-vivo perfused porcine bladder, as well as to describe the relationship between micromotion and afferent nerve signaling. Porcine bladders were reanimated using ex-vivo perfusion with a physiologic buffer. The pelvic nerve adjacent to the bladder was dissected, grasped with micro-hook electrodes and electroneurogram (ENG) signals were recorded at 20 kHz. Bladders were catheterized and intravesical pressure measurements were taken using a Laborie XT Urodynamics system. Bladders were filled to a fixed volume of 300 mL and control measurements were recorded. The bladders were then washed with 0.001 M carbachol (CCh) solution and refilled to 300 mL to induce micromotion, which was detected as rhythmic changes in intravesical pressure. ENG amplitude was calculated in μV, and nerve firing rate was calculated as number of spikes above baseline threshold per minute. Micromotion was induced by carbachol in 12/25 (48.4%) of trials as rhythmic changes in intravesical pressure after the instillation of carbachol but not in any control period. A fast Fourier transform (FFT) algorithm showed average peak dominant frequency component amplitude was significantly higher during the carbachol period when compared to the control period (0.47 vs. 0.01 cm-H2O, p < 0.0001). Peak waveform frequency (1.13 vs. 1.54 cycles/min, p > 0.05) did not differ between control and carbachol periods. With regard to afferent nerve signaling, normalized average amplitude (0.66 ± 0.24 vs. 0.05 ± 0.08 μV) and firing rate (0.68 ± 0.28 vs. 0.18 ± 0.22 spike/min) for all bladders was significantly greater in the carbachol period when compared to the control period (p < 0.001). Micromotion can be induced using instillation of carbachol in a perfused ex-vivo porcine bladder. Increased afferent nerve firing is observed during periods of micromotion. Thus, micromotion may drive afferent nerve signaling and may potentially contribute to urinary urgency, detrusor overactivity, and OAB. The development of an experimental ex-vivo porcine model for micromotion provides a reproducible method to study bladder micromotion and its potential role in the pathophysiology of urinary urgency and voiding dysfunction.

Optimized Airborne Millimeter-Wave InSAR for Complex Mountain Terrain Mapping

The efficient acquisition and processing of large-scale terrain data has always been a focal point in the field of photogrammetry. Particularly in complex mountainous regions characterized by clouds, terrain, and airspace environments, the window for data collection is extremely limited. This paper investigates the use of airborne millimeter-wave InSAR systems for efficient terrain mapping under such challenging conditions. The system’s potential for technical application is significant due to its minimal influence from cloud cover and its ability to acquire data in all-weather and all-day conditions. Focusing on the key factors in airborne InSAR data acquisition, this study explores advanced route planning and ground control measurement techniques. Leveraging radar observation geometry and global SRTM DEM data, we simulate layover and shadow effects to formulate an optimal flight path design. Additionally, the study examines methods to reduce synchronous ground control points in mountainous areas, thereby enhancing the rapid acquisition of terrain data. The results demonstrate that this approach not only significantly reduces field work and aviation costs but also ensures the accuracy of the mountain surface data generated by airborne millimeter-wave InSAR, offering substantial practical application value by reducing field work and aviation costs while maintaining data accuracy.

Basics of high throughput sequencing Summary

Sequencing can provide genomic characterisation of a specific organism, as well as of a whole environmental or clinical sample. High Throughput Sequencing (HTS) makes it possible to generate an enormous amount of genomic data at gradually decreasing costs and almost in real-time. HTS is used, among others, in medicine, veterinary medicine, microbiology, virology and epidemiology. The paper presents practical aspects of the HTS technology. It describes generations of sequencing, which vary in throughput, read length, accuracy and costs ̶ and thus are used for different applications. The stages of HTS, as well as their purposes and pitfalls, are presented: extraction of the genetic material, library preparation, sequencing and data processing. For success of the whole process, all stages need to follow strict quality control measurements. Choosing the right sequencing platform, proper sample and library preparation procedures, as well as adequate bioinformatic tools are crucial for high quality results.

Agreement between self-reported and objectively measured hypertension diagnosis and control: evidence from a nationally representative sample of community-dwelling middle-aged and older adults in China.

As the population ages, hypertension has become the leading risk factor for cardiovascular diseases (CVDs) and premature deaths worldwide. Accurate monitoring of CVD risks and planning community-based public health interventions require reliable estimates of hypertension prevalence and management. While the validity of self-reporting in assessing hypertension prevalence has been debated, the concordance between self-reports and clinical measurements of hypertension control remains underexplored, particularly in large, community-based older populations. This study aims to examine the agreement between self-reported and objectively measured data on both hypertension diagnosis and control, as well as the associated factors, among community-dwelling middle-aged and older Chinese adults. Data from the China Health and Retirement Longitudinal Study were utilized, with household survey responses combined with biomedical data. Sensitivity, specificity, and kappa coefficients were used to assess the agreement between self-reported and objectively measured hypertension diagnosis in the general sample, and the agreement on hypertension control among individuals who reported having hypertension. Binary and multinomial logistic regression analyses were conducted to identify individual, household, and community-level factors associated with the agreement. Self-reports exhibited substantial sensitivity, excellent specificity, and moderate agreement with objective measurements for hypertension diagnosis, while demonstrating fair sensitivity, excellent specificity, but low agreement for hypertension control. The odds of agreement on hypertension diagnosis were negatively associated with older age and heavy drinking, but positively related to marital status, higher education, chronic kidney disease, recent healthcare service utilization, and higher household economic levels. Meanwhile, the likelihood of agreement on hypertension control was negatively associated with older age, comorbid diabetes or cardiovascular disease, heavy drinking, BMI over 25, and antihypertensive medication adherence, but positively associated with recently healthcare service utilization. Self-reporting underestimated hypertension prevalence but significantly overestimated the hypertension control rates. For middle-aged and older Chinese adults, individual-level factors including age, multimorbidity, behavioural risks, and healthcare-seeking behaviours were identified as significant predictors of agreement between self-reported and objectively measured hypertension data. Recognizing these factors is essential for improving the accuracy of chronic condition estimates and facilitating targeted chronic disease management programs for China's aging population and other developing countries with similar demographic and health challenges.

Muscle Tone and Stiffness Comparison in Ambulatory Children With Unilateral Spastic Cerebral Palsy: Implications for Postural Balance and Functional Mobility.

To compare muscle tone and stiffness in ambulatory children with unilateral spastic cerebral palsy (UCP) with typically developing peers and explore their relationship with postural balance and functional mobility. Forty ambulatory children with UCP and age-matched typically developing peers were assessed for tone and stiffness of lumbar spinal extensors, gastrocnemius, and hamstring muscles using a myotonometer. Functional mobility was evaluated with the 2-Minute Walk Test, and the Timed Up and Go Test, while postural balance was evaluated using the Pediatric Balance Scale and the Trunk Control Measurement Scale (TCMS). The gastrocnemius muscle tone and stiffness were higher on the affected side in UCP compared with the less affected side and typically developing peers (P < .05). Lumbar spinal extensor tone correlated with improved Trunk Control Measurement Scale scores (P = .003). The gastrocnemius and hamstring muscles' tone and stiffness did not significantly affect functional mobility measures in UCP (P > .05). Our study highlights the importance of achieving muscle symmetry, particularly in the plantar flexors, for functional mobility in UCP children. While differences in ankle and knee muscle biomechanics were observed, they didn't significantly impact functional mobility or postural balance. Symmetry in lumbar spinal extensor biomechanics correlated with better outcomes, emphasizing the crucial role of trunk control in rehabilitation strategies for ambulatory children with UCP.

In People with Subacute Mild Traumatic Brain Injury, earlier Physical Therapy Improved Symptoms at a Faster Rate than Later Physical Therapy: Randomized Controlled Trial.

There is unclear evidence on when to initiate physical therapy after mild traumatic brain injury (mTBI) in non-athlete, adult population. The objective of this study was to investigate physical therapy timing after mTBI through changes in patient-reported and clinically-assessed tools and objective and mechanism measurements of sensorimotor balance control. This study was an investigator-blinded randomized control trial (NCT03479541). The study took place at an academic research center. Two hundred and three participants were randomized to earlier physical therapy (n = 82) or to later physical therapy (n = 121). After enrollment, the earlier physical therapy group started rehabilitation within 1week and the later group started rehabilitation after a 6-week wait period. All participants received similar rehabilitation; 6-week program administered and progressed by licensed physical therapists. The primary outcome was the Dizziness Handicap Inventory (DHI). Secondary outcomes included common patient-reported/clinical assessments of mTBI and objective/mechanism measurements of balance, including novel measures of central sensorimotor integration. Differences between and within the groups on outcomes were examined with linear mixed-effect models, t tests, and effect sizes. While both groups significantly improved and reached similar levels on patient-reported outcomes (DHI and secondary outcomes), the earlier physical therapy group had significantly larger and faster rates of improvement compared to later physical therapy. There were differential effects of physical therapy timing on the objective/mechanism-measured outcomes. Specifically, there were significant improvements in sensorimotor time delay for the earlier physical therapy group and no change in the later group. Further, the later group worsened in the motor activation components for balance control while there was no change in the early group. Earlier physical therapy after mTBI can improve symptoms at a faster rate relative to later physical therapy. Earlier physical therapy also showed improvements in sensorimotor aspects of balance control, not seen in the later group. There may be an important window to address central sensorimotor deficits after mTBI.

The Relationship Between Selective Motor Control and Trunk Control in Children With Spastic Cerebral Palsy

Aim: Cerebral palsy (CP) can cause a variety of musculoskeletal issues that impact everyday functioning and activities, including reduced muscle tone and selective motor control. Appropriate evaluation of these problems and determination of their interrelationships are important in treatment planning. The aim of this study was to investigate the correlation between control of the trunk and selectivity of the lower limbs in children with CP. Material and Method: Sixty-eight children and adolescents with spastic CP, categorized as GMFCS levels I to III and aged between five and seventeen years, were enrolled in the present study. The Trunk Control Measurement Scale (TCMS) was utilized to evaluate the trunk control. The Selective Control Assessment of the Lower Extremity (SCALE) was performed to measure the selectivity of the lower extremity. Groups with varying GMFCS levels were analyzed using the Kruskal-Wallis test. For the relationship analysis, the Spearman rank correlation test was employed. Results: Significant differences in total SCALE scores between levels of the GMFCS were found in the group comparisons (Kruskal-Wallis H test: 44.145, p

Low-Dose Oral Ginger Improves Daily Symptom Scores in Asthma.

Background/Objective: A significant number of individuals with asthma have poorly controlled daily symptoms and utilize dietary supplements such as ginger in a quest for improved symptom control; however, its effectiveness at improving the control of symptoms is unproven. We questioned whether low-dose oral ginger would improve subjective and objective measurements of asthma control in mild-to-moderate asthmatics. Methods: We performed a randomized, placebo-controlled, double-blinded study of a low dose (1 g twice daily) of a dietary supplement of ginger in 32 mild-to-moderate uncontrolled asthmatics over a 2-month trial period while maintaining daily conventional asthma therapies. The planned primary outcomes included an increased tolerance to inhaled methacholine and decreased concentrations of fractional excretion of exhaled nitric oxide (FeNO). Secondary planned outcomes included measurements of asthma control by the Asthma Control Test (ACT), a 2-week symptom recall test, and the Juniper mini Asthma Quality of Life Questionnaire (AQLQ), and blood eosinophils and asthma-associated cytokines. Results: Exhaled nitric oxide or blood eosinophils were not changed by oral ginger. However, three different measures of asthma symptom control were improved by the 28-day time point of oral ginger. Asthma-associated serum cytokines (IL-13 and IL-17A) were modulated by oral ginger. Conclusions: This is the first demonstration that a small daily dose of a dietary supplement of ginger may improve asthma symptoms and reduce inflammation in human asthmatics. These findings support the need for additional studies using larger doses of ginger in specific endotypes of asthmatics that may identify a novel therapeutic for asthma.

Effects of abdominal muscle strengthening and pelvic control exercises on trunk control and pelvic movement in children with unilateral cerebral palsy.

Children with unilateral cerebral palsy (CP) exhibit abnormal movements due to atypical pelvic movements and weakness of trunk muscles. We investigated the effects of abdominal muscle strengthening and pelvic control exercises on trunk control, abdominal muscle thickness change rate, and pelvic movement in children with unilateral CP. Fourteen children with unilateral CP were randomly divided into two groups, and abdominal muscle strengthening and pelvic control exercises were applied to the experimental group, and general physical therapy was applied to the control group, 30 min per session, twice a week, for a total of 8 weeks, respectively. The trunk control measurement scale (TCMS) was used to measure trunk control, ultrasonography was used to measure abdominal muscle thickness change rate, and a wearable gait analysis system was used to measure pelvic movement. As a result, in the experimental group, TCMS showed a significant increase within the group, and the rotation range of the pelvis significantly decreased within the group on the affected and nonaffected sides. There was a significant difference between the groups in the abdominal muscle thickness change rate of the internal oblique on the affected side. Improving trunk control through abdominal strengthening and pelvic control exercises affected the deep muscles on the affected side and contributed to a reduction in excessive pelvic rotation range. Therefore, abdominal muscle strengthening and pelvic control exercises may be suggested as intervention methods to improve function in unilateral CP.

Effects of 1267 nm Illumination on Microcirculation Regulatory Mechanisms.

This study explored the effects of 1267 nm laser irradiation on changes in blood flow parameters and activation of the regulatory mechanisms of the microcirculatory bed (MCB). Using laser Doppler flowmetry (LDF) technique and time-frequency analysis of perfusion signals, changes in the MCB of 16 healthy volunteers, targeting the distal phalanx of the third finger with 1267 nm laser irradiation were evaluated. Results indicated no significant differences in perfusion between control and target measurements, likely due to blood flow redistribution caused by vessel dilation/constriction. However, differences in oscillation amplitudes in endothelial and myogenic ranges were observed, suggesting microcirculation self-regulation. Detailed analysis revealed characteristic peaks in the endothelial range during and after irradiation, indicating endothelial mediator release. It is assumed that the identified effects may be related to the singlet oxygen generated by 1267 nm laser irradiation, which directly affects the MCB, manifesting as endothelium-dependent vascular activity.

Mathematical Modeling and Enhancing the Carrying Capacity of National Parks in the United States

A rapid growth in the number of visitors has been observed for many national parks in the United States, examples include Yellowstone National Park, Yosemite National Park, Rocky Mountain National Park and Arches National Park. As a result, public transit and parking resources have been stretched closer and closer to their capacities in these parks. In response, some National Park Services (NPS) have started to implement crowd control measurements, such as the timed entry reservation systems, where visitors have to reserve their slots to visit the park during certain time periods of the year. However, such policy does not touch on the fundamental issue of the subject, that is, the carrying capacity of the park. In this paper, we demonstrate that there will be even greater growth in the number of visitors in the near future, which means that a capacity boost is inevitable. Fortunately, we also observe that if we can fix some key bottlenecks, then we can dramatically increase the capacity of the park without destroying more natural land.

A chip thermal management method realizing integrated applications of cooling, power generation and heat flow measurement based on thermoelectric effect

Thermoelectric cooling, power generation and heat flow measurement are widely used in frontier domains while each of them is still in an isolated state. They are now widely used in fields such as electronic cooling, wearable device, sensors and so on. This paper proposes a method of thermoelectric effect combined with pulse width modulation (PWM) to achieve integrating thermoelectric applications. In a PWM cycle, the integration of thermoelectric applications makes it possible to realize three different operations of temperature control, energy recovery and heat flow measurement through a single thermoelectric module. The experimental results indicate that increasing the input voltage of the thermoelectric module extends the duration for power generation and heat flow measurement, while also enhancing the energy recovery rate. When the target temperature is higher than the ambient temperature, although the temperature control accuracy slightly decreases with the increase of the target temperature, the average temperature fluctuation range of the whole experimental group is −0.09∼+0.1℃ and energy recovery rate increases by 79.9 %. When the target temperature is lower than the ambient temperature, the system can still achieve efficient energy recovery. Applying the method to the computer central processing unit(CPU), the experimental results present that the heat dissipation of CPU can be monitored by measuring heat flow. Compared with the CPU + FPU working, the total power generation is increased by 0.285 W and energy recovery rate is increased by 78.3 % when the CPU is idle.

Suitability of UR5 Robot for Robotic 3D Printing

The present paper describes the measurement of the drift of unidirectional pose accuracy, repeatability, and static compliance of a collaborative robot employing a measurement methodology that relies on the description of a virtual ISO cube placed in the robot’s workspace. The measurements aimed to investigate and assess the suitability of the UR5 six-axis collaborative robot for its application in robotic 3D printing. An experimental laboratory measurement workstation was constructed to perform the measurements, and control measurements were performed. The measurements involved describing the TCP point of the robot tool at five measurement points located in a virtual ISO cube during a minimum of 30 repeated measurement cycles. A camera and six linear incremental sensors with assessment units were used for the measurements. The measurements were performed in compliance with the regulations of STN ISO 9283 standard for this type of measurement. As a result of the measurements, the technical specifications of the drift and static compliance of the controlled robotic arm were verified, and the results were compared with the values specified by the manufacturer. Following the measurements and assessment of the results, it was possible to assess the suitability of the used UR5 robotic arm for its application in robotic 3D printing and to propose possible recommendations for the calibration of the robot and the process settings of the printing system for the production of objects using FDM technology.

Synergistic Passivation of Bulk and Interfacial Defects Improves Efficiency and Stability of Inverted Perovskite Solar Cells

Defects both in bulk and at the interfaces serve as charge trapping sites for nonradiative recombination and as ion migration pathways, resulting in degradation of perovskite solar cell efficiency and stability. In this work, a strategy for simultaneous passivation of both bulk and interfacial defects is reported. For bulk passivation polystyrene (PS) is used as an additive in the perovskite precursor which reduces the structural defects by forming larger defect‐free grains. While the F‐PEAI cation is used to passivate the interfacial defects, present at both perovskite HTL/ETL interfaces. Furthermore, by conducting control measurements with just bulk modification (PS), just interface modification (F‐PEAI), and a combination of both, the role of individual defect passivation strategies is decoupled. As a result of simultaneous bulk as well as interfacial passivation, the modified perovskite solar cell shows the highest efficiency of 22.32% with a high Voc of 1.14 V and fill factor of 80%. Moreover, the cells have excellent stability retaining 92% and 99% of their initial efficiency after 1008 h and 560 h under ISOS‐ D1 and D2 storage conditions. These results highlight the importance of simultaneous bulk and interfacial passivation for improving solar cell efficiency and stability.

Accelerated construction and geometry control of cable-stayed bridge: cable crane method

Mountainous regions with rugged terrain, steep valleys and restricted access to roads and rivers present distinctive challenges for segmented erection of cable-stayed bridges (CSBs). In recent years, the cable crane method (CCM) has emerged as an innovative solution for CSB construction to address the difficulties associated with vertically lifting and horizontally delivering girder segments in such challenging environments. However, integrating the CCM with a CSB introduces a coupling effect, significantly complicating the control of bridge geometry. In this work, the accelerated construction and geometry control for CSBs employing the CCM in mountainous regions were systematically studied. The characteristics of 1521 bridges in mountainous regions were investigated, followed by a numerical analysis of geometry control and field measurements of as-built geometries, forces and ambient conditions during construction. Critical techniques for CSB construction using the CCM are also clarified. The mechanical behaviour of a bridge during construction was comprehensively evaluated to improve geometry control. The numerical results were validated through field measurements, offering valuable insights for future CSB practices using the novel CCM.

Joint optimal PMU and DULR placement in distribution network considering fault reconstruction and state estimation accuracy

Due to the rapid development of the distribution network, there is an urgent need for high-precision state estimation and safe and stable operation, this paper proposes a joint optimal placement method for Phasor Measurement Unit (PMU) and Dual Use Line Relay (DULR) in distribution networks considering fault reconstruction and state estimation accuracy. The proposed method aims to minimize the cost of PMU and DULR configuration. Based on the known pseudo-measurements and Supervisory Control and Data Acquisition (SCADA) measurements, we use the E-optimal standard design of the state estimation error covariance matrix to form the state estimation accuracy index and take the relay protection function of Feeder Terminal Unit (FTU) device and DULR device into consideration of the fault reconstruction. By solving the mixed integer semidefinite programming (MISDP) model, the optimal placement result is obtained, so that the network under normal condition and the network after multiple fault reconstruction can meet the constraints of state estimation accuracy and load loss rate respectively. The proposed method is tested on IEEE 33-node, 123-node systems as an example. The experimental results show that the proposed method is effective and practical.Note to Practitioners—This study is stimulated by the need for optimizing the placement of Micro-PMU (µPMU) in distribution network. Since the new µPMU device DULR has the same relay protection function as FTU, and distributed generation can supply power to the island after failure, this paper adds the load loss rate to the constraint conditions to study the influence of fault reconstruction on the placement scheme. A new two-step SE method considering mixed measurements was used in this paper to improve the computational efficiency of real-time SE of large-scale system while ensuring the accuracy of the calculation results. We have conducted tests in IEEE-33 and IEEE-123 systems. After preliminary verification, compared with the single fault scenario, we find that the placement scheme considering multiple fault scenarios can effectively improve the accuracy of state estimation and reduce the load loss rate of each fault scenario and only increase the cost a little. The increase in the number of DG and contact lines in the network will also effectively improve the feasibility of the placement scheme. The increase of the number of FTU configurations can effectively reduce the configuration of DULR by adding a small amount of µPMU, reduce the total cost as a result. In future studies, we will further consider the integrated configuration of µPMU, DULR and FTU, to meet more failure scenarios while reducing costs.

Effect of exercise alone and in combination with time-restricted eating on cardiometabolic health in menopausal women

There is a need to investigate the effect of lifestyle modifications on cardiometabolic health-related issues that occur during menopause. The aim of this study was to compare the effect of resistance and endurance circuit training program alone (exercise group, n = 34) with the effect of time-restricted eating (16:8) combined with a training program (combination group, n = 28) on cardiometabolic health in 62 menopausal women (aged 51.3 ± 4.69 years). Testing was conducted before and after a 12-week period and included an assessment of body composition, glycemic control, lipid panel, blood pressure, and anthropometric measurements. Decreases in body mass index and systolic blood pressure were significantly greater in the combination group than in the exercise group (F(1,60) = 4.482, p = 0.038, η2 = 0.07; F(1,57) = 5.215, p = 0.026, η2 = 0.08, respectively, indicating moderate effects). There were significant decreases in fat mass (p = 0.001, r = 0.654), glucose level (p = 0.017, r = 0.459), insulin level (p = 0.013, r = 0.467), homeostatic model assessment for insulin resistance (p = 0.009, r = 0.499), waist circumference (p = 0.002, r = 0.596), and waist-to-height ratio (p = 0.003, r = 0.588) (indicating moderate effect) in the combination group, while there were no significant changes in the exercise group. There were no changes in lipid panel indicators in either group. This is the first study to investigate the effect of time-restricted eating combined with exercise in menopausal women. The results of the study provide evidence that the combination of time-restricted eating and exercise leads to a greater body mass index reduction than exercise alone in menopausal women.Trial registration: ClinicalTrials.gov, NCT06138015 registered 18 November 2023—Retrospectively registered, https://clinicaltrials.gov/study/NCT06138015.

Temperature correction of near-infrared spectra of raw milk

Accurate milk composition analysis is crucial for improving product quality, economic efficiency, and animal health in the dairy industry. Near-infrared (NIR) spectroscopy can quantify milk composition quickly and nondestructively. However, external factors, such as temperature fluctuations, can alter the molecular vibrations and hydrogen bonding in milk, altering the NIR spectra and leading to errors in predicting key constituents such as fat, protein, and lactose. This study compares the effectiveness of Piecewise Direct Standardization (PDS), Continuous PDS (CPDS), External Parameter Orthogonalization (EPO), and Dynamic Orthogonal Projection (DOP in correcting the impact of temperature-induced variations on predictions in milk long-wave NIR spectra (LW-NIR, 1000–1700 nm).A total of 270 raw milk samples were analyzed, collecting both reflectance and transmittance spectra at five different temperatures (20 °C, 25 °C, 30 °C, 35 °C, and 40 °C). The experimental setup ensured precise temperature control and accurate spectral measurements. PLSR models were calibrated at 30 °C to predict milk fat, protein, and lactose content. The performance of these models was assessed before and after applying the temperature correction methods, with a primary focus on reflectance spectra.Results indicate that EPO and DOP significantly enhance model robustness and prediction accuracy across all temperatures, outperforming PDS and CPDS, especially for lactose prediction. These orthogonalization methods were compared against PLSR models calibrated with spectra from all temperatures. EPO and DOP showed comparable or superior performance, highlighting their effectiveness without requiring extensive temperature-specific calibration data. These findings suggest that orthogonalization methods are particularly suitable for in-line milk quality measurements under farm conditions where temperature control is challenging. This study highlights the potential of advanced chemometric techniques to improve real-time, on-farm milk composition analysis, facilitating better farm management and enhanced dairy product quality.

Cellulose nanomaterial metrology: microscopy measurements.

Cellulose nanomaterials are increasingly used for a wide variety of applications. Adequate characterization of these materials is required for quality control during production, to distinguish between materials synthesized by different methods, by different suppliers or from difference cellulose biomass sources, to facilitate development of applications and for regulatory purposes. Here we review recent microscopy measurements for the three main types of cellulose nanomaterials: cellulose nanocrystals, individual cellulose nanofibrils and cellulose nanofibrils. Atomic force microscopy and both scanning and transmission electron microscopy are covered with a focus on recent studies that have metrological rigor, rather than qualitative investigations. In some cases results are compared to those obtained by other methods that are more likely to see widespread use for routine quality control measurements. Detailed studies that use microscopy to provide insight on fundamental material properties (e.g., chiral properties) are also included. Particle size and morphology are important properties but are challenging to measure for cellulose nanomaterials due to the rod or fibril shaped particles, their propensity to agglomerate and aggregate, their low contrast for electron microscopy and, for cellulose nanofibrils, the complex branched and interconnected structures. Overall, the results show that there are now a number of studies in which attention to metrological detail has resulted in measurements that allow one to compare and distinguish between different materials, although there are still examples for which it is not possible to draw conclusions on size differences. The use of detailed microscopy protocols that yield accurate and reliable results will be beneficial in material production and addressing regulatory requirements and will allow the validation of other methods that are more amenable to routine measurements.

Influence of Polymer Film Thickness on Drug Release from Fluidized Bed Coated Pellets and Intended Process and Product Control.

Coated drug pellets enjoy widespread use in hard gelatine capsules. In heterogeneous pellets, the drug substance is layered onto core pellets. Coatings are often applied to generate a retarded release or an enteric coating. In the present study, the thickness of a polymer coating layer on drug pellets was correlated to the drug release kinetics. The question should be answered whether it is possible to stop the coating process when a layer thickness referring to an intended drug release is achieved. Inert pellets were first coated with sodium benzoate and second with different amounts of water insoluble polyacrylate in a fluidized bed apparatus equipped with a Wurster inlet. The whole process was controlled in-line and at-line with process analytical technology by the measurement of the particle size and the layer thickness. The in-vitro sodium benzoate release was investigated, and the data were linearized by different standard models and compared with the polyacrylate layer thickness. With increasing polyacrylate layer thickness the release rate diminishes. The superposition of several processes influencing the release results in release profiles corresponding approximately to first order kinetics. The coating layer thickness corresponds to a determined drug release profile. The manufacturing of coated drug pellets with intended drug release is possible by coating process control and layer thickness measurement. Preliminary investigations are necessary for different formulations.