Electricity generation in Islanded Urban Microgrids (IUMG) now relies heavily on a diverse range of Renewable Energy Sources (RES). However, the dependable utilization of these sources hinges upon efficient Electrical Energy Storage Systems (EESs). As the intermittent nature of RES output and the low inertia of IUMGs often lead to significant frequency fluctuations, the role of EESs becomes pivotal. While these storage systems effectively mitigate frequency deviations, their high costs and elevated power density requirements necessitate alternative strategies to balance power supply and demand. In recent years, substantial attention has turned towards harnessing Electric Vehicle (EV) batteries as Mobile EV Energy Storage (MEVES) units to counteract frequency variations in IUMGs. Integrating MEVES into the IUMG infrastructure introduces complexity and demands a robust control mechanism for optimal operation. Therefore, this paper introduces a robust, high-order degree of freedom cascade controller known as the 1PD-3DOF-PID (1 + Proportional + Derivative—Three Degrees Of Freedom Proportional-Integral-Derivative) controller for Load Frequency Control (LFC) in IUMGs integrated with MEVES. The controller’s parameters are meticulously optimized using the Coati Optimization Algorithm (COA) which mimics coati behavior in nature, marking its debut in LFC of IUMG applications. Comparative evaluations against classical controllers and algorithms, such as 3DOF-PID, PID, Reptile Search Algorithm, and White Shark Optimizer, are conducted under diverse IUMG operating scenarios. The testbed comprises various renewable energy sources, including wind turbines, photovoltaics, Diesel Engine Generators (DEGs), Fuel Cells (FCs), and both Mobile and Fixed energy storage units. Managing power balance in this entirely renewable environment presents a formidable challenge, prompting an examination of the influence of MEVES, DEG, and FC as controllable units to mitigate active power imbalances. Metaheuristic algorithms in MATLAB-SIMULINK platforms are employed to identify the controller’s gains across all case studies, ensuring the maintenance of IUMG system frequency within predefined limits. Simulation results convincingly establish the superiority of the proposed controller over other counterparts. Furthermore, the controller’s robustness is rigorously tested under ± 25% variations in specific IUMG parameters, affirming its resilience. Statistical analyses reinforce the robust performance of the COA-based 1PD-3DOF-PID control method. This work highlights the potential of the COA Technique-optimized 1PD-3DOF-PID controller for IUMG control, marking its debut application in the LFC domain for IUMGs. This comprehensive study contributes valuable insights into enhancing the reliability and stability of Islanded Urban Microgrids while integrating Mobile EV Energy Storage, marking a significant advancement in the field of Load-Frequency Control.