Control Diet Diet Research Articles

Expression of chicken epidermal growth factor (cEGF) in Escherichia coli regulates the microflora structure of the duodenum to improve growth performance and intestinal morphogenesis in broilers

ABSTRACT 1. A study used gene synthesis to obtain the functional domains of chicken epidermal growth factor (cEGF) and examined their impact on broiler growth performance, small intestinal morphology, digestive enzyme activities in the intestinal contents and the structure of duodenal microflora. 2. The pET-32a-cEGF recombinant expression vector was constructed. The specific band at 26 KDa was shown by SDS-PAGE analysis and WB results. The purified protein content was shown to be 1687 μg/ml by assay. 3. A total of 180 healthy, one-day-old Arbor Acres male, white-feathered broilers were randomly divided into three dietary treatment groups (six replicate pens, 10 birds per replicate): A control diet (ND); cEGF diet (cEGF), control supplemented with 250 mg/kg cEGF and the control diet (CD) supplemented with 250 mg/kg chlortetracycline. 4. The results showed that feeding the cEGF and CD diet reduced FCR of broilers aged 1–21 d, average daily feed intake (ADFI) at 22–42 d, and the FCR in the whole period (1–42 d; p < 0.05). Compared with the ND group, the cEGF diet increased duodenal α-amylase and alkaline phosphatase activities in the 1–21 d, duodenal lipase, alkaline phosphatase, and ileal alkaline phosphatase activities in the post-period and increased villus height in the duodenum and ileum (p < 0.05). In addition, the ACE and Chao1 index for the birds fed cEGF were higher than the ND group (p < 0.05). At the phyla level, Firmicutes and Proteobacteria were dominant in all groups. At the genus level, the dominant genus was Lactobacillus. The LEfSe analysis showed that the cEGF group was enriched by 11 species including Brevibacillus, Eisenbergiella, Cloacibacterium, Butyricoccus spp. 5. The addition of 250 mg/kg cEGF to the diet can increase growth performance by improving intestinal development and digestive enzyme activity, which may be related to the duodenal intestinal microflora. Therefore, cEGF is an effective alternative to antibiotics in broiler farming.

PSI-19 Neonatal Lamb Growth and Thermoregulation After Nutrient Specific Restriction in Gestation

Abstract Depending on seasonal availability and quality of range forage, extensively managed ewes can experience nutrient restriction during any point of gestation. Neonatal lamb performance after severe experimental nutrient restriction during gestation has been well studied; however, there is a gap in understanding of how practical nutrient restriction experienced over the course of a production cycle impacts lamb success. Therefore, the objective of this study was to investigate the first week fetal growth and development, and thermoregulation biomarkers in neonatal lambs from ewes fed a diet that simulates winter forage. Time-mated and confirmed pregnant Rambouillet x Merino ewes were blocked by body weight and allocated to one of two dietary treatments. Treatments consisted of 1) Control diet (CONT) formulated to meet or exceed the National Research Council (NRC) nutrient requirements for gestating ewes fed throughout gestation; 2) Nutrient restricted diet (NR) formulated to meet 70% of total digestible nutrient (TDN) and 50% of protein requirements from 30 to 125 d of gestation (dGA) and then re-alimented with the CONT diet for the remainder of gestation. Ewes were individually fed from 25 dGA to 137 dGA, then group fed through lambing. From 30 dGA to lambing, weekly body weights, bimonthly body condition scores (BCS), and monthly blood glucose concentrations were recorded for all ewes. A treatment by time interaction was observed where NR ewes weighed less (P &amp;lt; 0.05) starting at week 8 of the treatment period through lambing. There were effects of treatment and time on BCS, as ewes receiving the NR diet had lesser (P &amp;lt; 0.05) BCS compared with CONT ewes regardless of time in gestation. There were no observed differences in blood glucose concentrations between groups. At lambing, rectal temperature, birth weight and morphometrics (crown-rump length, abdominal circumference, head circumference, and cannon bone length) were collected on all lambs. Collection of rectal temperature and bodyweight continued daily for the first week of life. There were no differences in birthweight or birth morphometrics; however, growth by body weight in the first week of life was greater (P &amp;lt; 0.05) in NR (n = 10) compared with CONT lambs (n = 12), with an effect of progeny number. Additionally, treatment by day interactions were observed with NR lambs having lower (P &amp;lt; 0.05) body temperatures on days one, four, and five compared with CONT lambs. These data suggest that sustained nutrient challenge during gestation, even when re-alimented in late gestation, may produce lambs that are at increased risk of hypothermia. While maternal nutrient supplementation in late gestation is a common practice, it is crucial for producers to consider nutritional supplementation strategies throughout gestation to limit the number of lambs that require increased intervention after birth.

Effects of Dietary Supplementation of Oregano Bioactive Lipid Compounds and Silver Nanoparticles on Broiler Production

Feed additives could be added in the diets of broiler chickens to improve performance and for sustainable broiler production. The objective of this study was to evaluate the effectiveness of feeding broiler chickens with oregano bioactive lipid compounds (OBLC) and silver nanoparticles (Nano-Silver) on growth, viability, economics, carcass criteria, silver retentions, and serum biochemistry. One-day-old, unsexed Ross 308 broiler chicks (n = 320) were divided into four treatment groups. Ten replicates of eight birds each for each treatment were used. Treatments were: CONT (control diet), OBLC (CONT diet supplemented with 150 mg/kg OBLC), Nano-Silver (CONT diet supplemented with 4 mg/kg Nano-Silver), and CONT diet supplemented with OBLC + Nano-Silver at levels mentioned above were used for the study. The experiment lasted for 35 days. Supplementing OBLC and Nano-Silver individually or in combination improved (p &lt; 0.01) body weight and feed conversion ratio when compared to CONT. The supplemented groups had a lower death rate than the CONT group, resulting in a higher net return. Supplementing broiler birds with OBLC, Nano-Silver, or their combination boosted dressing percentage and decreased abdominal fat when compared to CONT. Blood levels of aspartate aminotransferase, alanine transaminase, creatinine, and urea were lowered in broilers fed OBLC, Nano-Silver, or combination diets. Overall, the current study demonstrates that broiler chickens fed diets with OBLC and Nano-Silver, either alone or in combination, improved growth performance, liver functions, dressing percent, silver absorption and decreased abdominal fat. The best performance was observed in the combination of OBLC and Nano-Silver group compared to other treatments.

Bacillus spp. Fermented Plant Protein Mix as a Potential Fishmeal Substitute in the Diet of Penaeus monodon: Emphasis on Nitrogen Metabolism

A feeding experiment (60 days) was conducted to evaluate the effect of Bacillus spp. fermented plant protein mix in Penaeus monodon. A control diet (CNT) contains 25% fishmeal, of which 50% was substituted (w/w) with Bacillus spp. fermented plant protein mix at two different concentrations of 0.25 and 0.50% (PP-0.25 and PP-0.50, respectively), while the diet prepared with untreated ingredients served as a negative control (PP-0). The daily growth coefficient (DGC) did not differ in the groups fed CNT, PP-0.25, and PP-0.50 diets. The feed conversion ratio (FCR) was low in the CNT and PP-0.50 diets, while (protein efficiency ratio (PER) and apparent protein utilization (APU) were high in the PP-0.50-fed group. The dietary change did not affect survival and whole-body composition, while free amino acids varied among the treatments. Increased nitrogen intake and oxygen consumption were observed in subadults fed with CNT diet. Increasing Bacillus spp. concentrations significantly lowered ammonia-N excretion by 2.09-11.69%. Regression analysis revealed higher coefficients for oxygen consumption ( R 2 = 0.8625 ) and O : N ratio ( R 2 = 0.7791 ), while it was 0.5894 for ammonia-N excretion. The dietary change significantly influenced hemolymph indices and chitinase, glutamate dehydrogenase (GDH), and arginase activity. Results conclude that Bacillus spp. fermented plant protein mix could be a viable fishmeal substitute in shrimp feed.

Parental high-fat high-sugar diet programming and hypothalamus adipose tissue axis in male Wistar rats.

Maternal nutrition during early development and paternal nutrition pre-conception can programme offspring health status. Hypothalamus adipose axis is a target of developmental programming, and paternal and maternal high-fat, high-sugar diet (HFS) may be an important factor that predisposes offspring to develop obesity later in life. This study aims to investigate Wistar rats' maternal and paternal HFS differential contribution on the development, adiposity, and hypothalamic inflammation in male offspring from weaning until adulthood. Male progenitors were fed a control diet (CD) or HFS for 10weeks before mating. After mating, dams were fed CD or HFS only during pregnancy and lactation. Forming the following male offspring groups: CD-maternal and paternal CD; MH-maternal HFS and paternal CD; PH-maternal CD and paternal HFS; PMH-maternal and paternal HFS. After weaning, male offspring were fed CD until adulthood. Maternal HFS diet increased weight, visceral adiposity, and serum total cholesterol levels, and decreased hypothalamic weight in weanling male rats. In adult male offspring, maternal HFS increased weight, glucose levels, and hypothalamic NFκBp65. Paternal HFS diet lowered hypothalamic insulin receptor levels in weanling offspring and glucose and insulin levels in adult offspring. The combined effects of maternal and paternal HFS diets increased triacylglycerol, leptin levels, and hypothalamic inflammation in weanling rats, and increased visceral adiposity in adulthood. Male offspring intake of CD diet after weaning reversed part of the effects of parental HFS diet during the perinatal period. However, maternal and paternal HFS diet affected adiposity and hypothalamic inflammation, which remained until adulthood.

Response of broiler chickens to jojoba oil and silver nanoparticles dietary supplementation

This study aimed to evaluate the effect of Jojoba Oil and nanoparticles of Silver supplementation on growth performance, viability and economics in broiler chickens. A total of 192 Ross 308 broiler chicks (1-day-old) were allocated to 4 experimental treatments: CONT (control diet), JO (CONT diet with 150 mg/kg of Jojoba oil), Nano-Ag (CONT diet with 4 mg/kg nanoparticles of Silver), and JO+Nano-Ag (CONT diet plus Jojoba Oil and nanoparticles of Silver). Each treatment had 6 replicates of 8 birds. The experiments lasted for 35 days. Starter and grower diets, based on corn and soybean meal, and water were available ad libitum. Body weight and feed intake were measured from 0 to 35 days. Body weight gain and feed conversion ratio were calculated. Survivability rate and cost economics were calculated. Statistical comparisons were performed using a One-way ANOVA. Supplementation of JO and Nano-Ag separately as well as in the form of combined usage, increased (P<0.05) body weight and body weight gain compared to CONT. Supplementation of broiler diets with JO, Nano-Ag or their combination improved feed conversion ratio, without any significant effect on feed intake. Interestingly, a lower mortality rate was recorded in the supplementation groups and, therefore, a higher net return was observed. Hence, the present study demonstrates that supplementation of JO and Nano-Ag separately as well as in the form of combined usage, improved growth performance and economic return of broiler chickens.

The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery.

Obesity and high-fat diet (HF) are prevalent causes of oxidative stress (OS). Duodenal-jejunal omega switch (DJOS) is a bariatric procedure used for body mass reduction, extensively tested in animal models. We studied the long-term impact of bariatric surgery and an HF diet on the oxidative stress markers in erythrocytes and heart muscles of rats. We analyzed superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) activity and malondialdehyde (MDA) concentration in DJOS or SHAM (control) operated rats fed with different dietary protocols (control diet (CD) and high-fat diet (HF)), before and after the surgery (CD/CD, HF/HF, CD/HF, and HF/CD). We observed higher erythrocytes CAT, GST and GPx activity in DJOS-operated (vs. SHAM) rats fed with an HF/HF diet. For DJOS-operated rats, erythrocytes CAT and GPx activity and MDA concentration were significantly lower in CD/CD group. We observed increased heart muscle GR activity in SHAM-operated rats (vs. DJOS bariatric surgery) fed with an HF/HF diet. Change from HF to CD diet increased heart muscle GPx activity after DJOS bariatric surgery. Heart muscle SOD activity was lower in HF/HF and CD/CD groups after DJOS bariatric surgery (vs. SHAM). DJOS surgery significantly reduced heart muscle MDA concentration in HF/HF and HF/CD groups (vs. SHAM). We conclude that the selected dietary patterns had a stronger impact on oxidative stress markers in erythrocytes and heart muscle than DJOS bariatric surgery.

P6274Role of the nicotinamide riboside kinase 2 in NAD metabolism in the heart in basal and pathological condition

Abstract Background NAD is a major coenzyme in energy metabolism and a substrate for SIRT1 and PARP1 enzymes involved in the response to energy and oxidative stress. We have shown the beneficial effects of nicotinamide riboside (NR), a new type of vitamin B3, on cardiac function and remodelling in a mouse model of dilated cardiomyopathy (DCM) triggered by deletion of the SRF transcription factor in the heart (Srf-HKO) (1). This functional improvement correlated with protection of NAD metabolism and a robust increase in cardiac expression of the Nicotinamide Riboside Kinase 2 (NMRK2) that phosphorylates NR to generate nicotinamide mononucleotide (NMN), an immediate precursor of NAD. Purpose We aim to understand the role of the NMRK2-mediated NAD biosynthetic pathway in the heart at baseline and in the DCM context. Methods We generated Nmrk2-KO mice that we bred with Srf-HKO to generate double KO mice (db-KO). We analysed cardiac function and remodelling by echocardiography and quantified myocardial NAD levels at baseline and following NR supplementation in food. Results Nmrk2KO mice developed a progressive eccentric remodelling of LV and decline in EF with aging. At 24-mo, we observed a reduction of myocardial NAD levels (−40% compared to wild type, p&lt;0.05) and of LVEF (61%, SD 6.3% in Nmrk2-KO vs 78%, SD 1.5% in WT, p&lt;0.05). To assess the contribution of cardiac Nmrk2 induction to NR response in DCM, we compared SrfH-KO and db-KO mice fed with control diet (CD) or NR supplemented diet for 40 days starting at young age (2-mo). NR reduced the extent of LV eccentric remodelling and drop in EF as well as the thinning of the LV posterior wall in both genotypes (2-way ANOVA, diet effect, p&lt;0.01). Myocardial NAD levels were more reduced in db-KO mice under CD diet (−22% compared to control mice, p&lt;0.05) than in Srf-HKO mice (−11%, non-significant), when we previously showed a 25% drop in myocardial NAD in aged SrfHKO mice (1). NR partially preserved cardiac NAD pool in db-KOmice (−10% compared to controls, non-significant). Parallel pathways for NMN synthesis were studied. Nampt gene expression was significantly repressed in db-KO mice fed with CD or NR diet compared to control mice (−50% in average, p&lt;0.01), when there was only a trend toward lower expression in SrfHKO mice (−40% in average, p&gt;0.05). Nmrk1 gene expression trended to increase in all groups compared to wild-type control mice. Conclusion We show that NMRK2 pathway plays a role in the maintenance of basal cardiac function and NAD levels when relying on the endogenous myocardial NR pool. In contrast, the beneficial effect of a therapeutic dose of NR is not affected by the lack of NMRK2 suggesting compensation by NMRK1 in the heart and/or that NR beneficial effects on cardiac function could be mediated through its action on systemic metabolism. Aging appears as an aggravating factor for the loss of myocardial NAD coenzyme in DCM. Acknowledgement/Funding Agence Nationale pour la Recherche, Fondation de France

PSVII-15 The effects of feeding a fortified protein blend as a replacement for animal and fish proteins in nursery pig diets

Abstract The objective of this study was to evaluate the effects of total or partial replacement of animal and fish proteins with a fortified protein blend (PROPLEX MVP) on growth performance of nursery piglets. PROPLEX MVP is a blend of two distinct fermentation biomass streams, refined soybean products, select amino acids, and a NSP enzyme complex. A total of 306 barrows and gilts [(Duroc x (Landrace x Yorkshire), avg. 22 d age and BW 6.37 ± 1.24 kg)] were used in a randomized complete block design with three dietary treatments, each with 19 replicates (pens) of 5 piglets. Dietary treatments were: 1) Control diet (CONT) with animal and fish protein sources (Blood meal, Spray-dried Plasma, and Fish meal); 2) PROPLEX MVP (MVP): CONT with MVP replacing Fish meal; 3) PROPLEX MVP 100 (MVP100): CONT with MVP replacing 100% of animal and fish proteins. Pigs were fed a budgeted amount per head of the assigned experimental diets for the first three feeding phases (Phase 1: 2.3 kg/pig; Phase 2: 4.5 kg/pig; Phase 3: 6.8 kg/pig). A common diet was provided in Phase 4 until day 42 post-weaning. Pigs fed CONT had greater ADG (P &amp;lt; 0.05) compared to MVP100 pigs d 0-7 post-weaning. From d 7 to 14, pigs fed MVP and MVP100 had a significantly greater ADG and G:F (P &amp;lt; 0.05) than pigs fed CONT diet. For the remainder of the experimental period (d 14 to 42), no differences (P &amp;gt; 0.10) were observed among treatments. The use of MVP100 reduced (P &amp;lt; 0.05) the total nursery feed cost by US $1.03/pig and feed cost/kg of weight gain by 12%. In conclusion, MVP protein blend can effectively substitute for animal and fish proteins sources without compromising overall nursery pigs’ growth performance and results in significant feed cost savings.

Transcriptomic Alterations of the Aortic Intima and Media in Long-term High-fat Diet Fed Pigs and Its Reversal (P15-010-19)

ObjectivesWe have previously shown that 12 months (mo.) high-fat diet (HFD) in pigs led to pathophysiological alterations, incl. fattening and increased femoral artery intima-media-thickness, which were partly reversed after 3 mo. return to control diet (Zabek et al., PLoS One 2017). The aim of this study was to decipher underlying mechanism of action of these dietary interventions on the arteries by nutrigenomics analyses of intima and media of aorta. Methods32 female pigs were divided into 3 groups: Control diet (CD) for 12 mo; HFD for 12 mo; 3) Reversal diet group (RD): HFD for 9 mo followed by CD for 3 mo After 12 mo animals were killed and abdominal aorta collected. RNA was isolated from aorta intima and media for whole genome microarray analyses followed by bioinformatics analyses. ResultsHFD compared to CD group significantly affected gene expression profile in intima with genes belonging to the chemotaxis, inflammation or endothelial permeability. RD induced gene expression profile was distinct from the CD group. This suggests that 3 mo of reversal to CD is not sufficient to correct gene expression changes induced by HFD. Comparison of RD profile with that of HFD group revealed a group of genes with opposite expression, e.g., genes regulating inflammation, toll-like cell signaling pathway or cytoskeleton organization involved in the regulation of cell permeability. This suggests that return to the RD only partly restored gene expression alterations due to the HFD. Significant changes in expression of genes in media following HFD were also observed, such as genes involved in cytoskeleton organization and migration MAPK signaling. As for intima, the expression profile of media of pigs on RD was different on that of these on CD diet. Compared to HFD, a group of genes involved in PI3K or MAPK pathways presented opposite expression suggesting that RD can partly correct the changes in genomic effect induced by HFD. ConclusionsThis study revealed genomic modifications induced by long-term HFD consumption on arterial intima and media. The return to normal diet for 3 mo was not sufficient to counteract the genomic effect of long-term HFD consumption. Funding SourcesWROVASC Integrated Cardiovascular Centre, co-financed by the European Regional Development Fund.

Comparison of The Effects of Dietary Supplementation of Natural Antimicrobial Feed Additives on Lipid Oxidation, Microbial Content and Quality of Broiler Raw Meat

The study aimed to compare the effects of dietary supplementation of probiotic and olive leaf-, grape seed- and pomegranate peel extracts as natural antimicrobial on lipid oxidation, microbiological content and quality of raw broiler meat. Chickens were fed the control diet (CONT) and diets supplemented with probiotic (P), oleuropein (olive leaf extract, OLE100 and OLE200), proanthocyanidin (grape seed extract, GSE100 and GSE200) and proanthocyanidin (pomegranate peel extract, PPE100 and PPE200) at 100 and 200 mg/kg levels to the CONT diet. All dietary treatments significantly reduced MDA value of breast meat at 9th day, total aerobe bacteria and coliform bacteria contents of breast meat at 14th day. The P, OLE200, PPE100 and PPE200 diets significantly decreased lactic acid bacteria content of breast meat at 14th day. The pH value of raw breast meat at 24 h was significantly reduced by dietary treatments compared to the CONT diet. Feeding the P, PPE100 and PPE200 diets significantly increased water holding capacity of breast meat compared to those of broilers fed the CONT, GSE100 and GSE200 diets. The P, OLE200, PPE100 and PPE200 diets significantly reduced drip loss of breast meat at 7th day compared to the CONT, OLE100, GSE100 and GSE200 diets. Cooking loss of breast meat was significantly decreased by all dietary treatments except GSE diet compared to the CONT diet. It was concluded that probiotic, olive leaf- and pomegranate peel- extracts have potential to be used as natural antimicrobial feed additives in terms of the lipid oxidation, microbial content and quality of broiler meat.

Impacts of maternal diet and exercise on offspring behavior and body weights

Human and animal studies indicate that maternal obesity can negatively impact aspects of metabolism and neurodevelopment in the offspring. Not known, however, is whether maternal exercise can alter these adverse outcomes. In this study, Long-Evans female rats were provided a high fat (60%; HFD) or control diet (CD) 44days before mating and throughout gestation and lactation. Running wheels were available to half of each diet group during the gestational period only, resulting in four conditions: CD diet with (CDRW) or without (sedentary; CDSED) exercise, and HFD with (HFRW) or without (HFSED) exercise. Only male offspring (one per litter) were available for this study: they were put on control diet two weeks after weaning and examined using behavioral evaluations up to four months of age. Before weaning, offspring of CDRW dams weighed less than offspring from CDSED or HFD dams. After weaning, the lower weight in CDRW offspring generally persisted. Adult offspring from HFSED dams performed worse than the HFRW group in a Morris water maze during initial spatial training as well as reversal learning; memory was not impacted. No differences between groups were seen in tests of novel object recognition, social approach, or chocolate milk preference. Thus, maternal diet and exercise produced differential effects on body weights and cognitive behaviors in the offspring, and the data demonstrate a positive impact of maternal exercise on the offspring in that it ameliorated some deleterious behavioral effects of a maternal high fat diet.

Effects of the Dietary Inclusion of a Probiotic, a Prebiotic or their Combinations on the Growth Performance of Broiler Chickens

A total of 350-one-day old chicks were placed in 70 cages, with 14 cages per treatment. The following five treatment diets were fed for 14 days: T1 = non-supplemented, control diet (CONT); T2 = diet with antimicrobial growth promoter (AGP); T3 = diet with a probiotic (PROB); T4 = diet with a prebiotic(PREB), and T5 = diet with the probiotic and the prebiotic (SYM). The growth experiment was carried out from 1 to 14 days of age. Feed and water were provided ad libitum and birds were maintained at 24-h light schedule. Diets were formulated to contain 3000 kcalME/kg and 21.5% crude protein, and the test materials were added on top. The cumulative results of1 to 14 days of age revealed that broiler fed the AGP and PREB diets presented the highest BWG (305.5 and 297.3 g, respectively), while those fed the CONT diet had the lowest BWG (273.2 g) (p<0.05). On the other hand, the best FCR was obtained in broilers AGP and PROB (1.296 and 1.299 g:g, respectively), while chicks on the CONT and SYM diet had the worst FCR (1.423 and 1.372 g:g, respectively) (p<0.01). The results showed broilers fed the non-supplemented diet consistently presented poor performance. It was concluded that PROB or PREB can serve as alternatives to antibiotic in broiler starter feeds, with no performance impairment.

Low-carbohydrate, high-fat diets have sex-specific effects on bone health in rats.

Studies in humans suggest that consumption of low-carbohydrate, high-fat diets (LC-HF) could be detrimental for growth and bone health. In young male rats, LC-HF diets negatively affect bone health by impairing the growth hormone/insulin-like growth factor axis (GH/IGF axis), while the effects in female rats remain unknown. Therefore, we investigated whether sex-specific effects of LC-HF diets on bone health exist. Twelve-week-old male and female Wistar rats were isoenergetically pair-fed either a control diet (CD), "Atkins-style" protein-matched diet (LC-HF-1), or ketogenic low-protein diet (LC-HF-2) for 4weeks. In females, microcomputed tomography and histomorphometry analyses were performed on the distal femur. Sex hormones were analysed with liquid chromatography-tandem mass spectrometry, and endocrine parameters including GH and IGF-I were measured by immunoassay. Trabecular bone volume, serum IGF-I and the bone formation marker P1NP were lower in male rats fed both LC-HF diets versus CD. LC-HF diets did not impair bone health in female rats, with no change in trabecular or cortical bone volume nor in serum markers of bone turnover between CD versus both LC-HF diet groups. Pituitary GH secretion was lower in female rats fed LC-HF diet, with no difference in circulating IGF-I. Circulating sex hormone concentrations remained unchanged in male and female rats fed LC-HF diets. A 4-week consumption of LC-HF diets has sex-specific effects on bone health-with no effects in adult female rats yet negative effects in adult male rats. This response seems to be driven by a sex-specific effect of LC-HF diets on the GH/IGF system.

Abstract 2150: Chemopreventive effects of black raspberries in endometrial cancer

Abstract Endometrial cancer is the most common type of gynecologic cancer and annually comprises 3% of all cancer deaths of women in the United States. Additionally, ∼80% of cases are type I endometrial cancer, mainly associated with Pten abnormalities. It was estimated that up to 90% of type I endometrial cancer patients are overweight/obese. Therefore, a regimen to prevent obesity associated tumorigenesis is the most important for type I endometrial cancer. In the current study, the Pten heterozygous (+/-) female mice were used as the model, to study the potentially chemopreventive effects by black raspberries (BRBs). At 3-4 weeks old, Pten (+/-) and wild type female mice were divided into four treatment groups, fed with either AIN-93G control diet (CD), CD with 5% BRBs, 58% fat to induce obesity (HF),or HF with 5% BRBs. All of mice were euthanized at 28 weeks old. In wild type mice, no malignant lesion was identified. In Pten (+/-) mice, HF diet increased 10% focal glandular hyperplasia with atypia lesions when compared with CD diet. Dietary administration of BRBs decreased 19% focal glandular hyperplasia with atypia lesions when compared with CD group. Specifically, BRBs in HF diet inhibited 32% obesity-induced endometrial malignant lesions and significantly lowed lesion multiplicity. Furthermore, cytokine profiles in plasma by Bio-Plex Assay revealed dietary BRBs decreased IL-1β and IL-17A, and lowed TNF-α and GM-CSF when compared with CD and HF group, respectively. In conclusion, this study for the first time suggested BRBs are chemopreventive for obesity-induced endometrial tumorigenesis. Citation Format: Jo-Hsin Chen, Irene Aguilera-Barrantes, Chieh-Ti Kuo, Leena Hilakivi-Clarke, Gary D. Stoner, Janet S. Rader, Yi-Wen Huang. Chemopreventive effects of black raspberries in endometrial cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2150. doi:10.1158/1538-7445.AM2014-2150

Maternal DHA Supplementation Improves Lung Growth and Function in Offspring Exposed to LPS and Hyperoxia

BackgroundMaternal inflammation contributes to low birth weight and preterm delivery. Premature infants routinely receive therapeutic oxygen and exhibit persistently diminished respiratory function into adulthood.ObjectiveWe tested the hypothesis that DHA supplementation reduces the detrimental effects of a hostile perinatal environment.MethodsPregnant C3H/HeN mice were fed DHA or control diet (CD) and injected with LPS or saline on embryonic day (E) 16. Offspring were placed in room air (RA) or 85% O2 for 14 d then returned to RA. PFTs, microCTs, molecular, and histological analyses were performed.Main ResultsBirth weight was reduced in pups born to CD/LPS dams but not different than controls in the pups born to DHA/LPS dams. At 8 weeks of age, bodyweights were lower, the lungs had fewer and larger alveoli, and static compliance was decreased in mice born to dams fed CD and exposed to LPS/O2. Maternal dietary supplementation with DHA in LPS/O2 exposed mice reversed these effects even at 8 weeks with bodyweights, alveolarization, and PFT measurements in not different than CD or DHA diet, saline injected, RA exposed controls.ConclusionsThe detrimental effects of systemic maternal inflammation and neonatal hyperoxia exposure on offspring lung structure and function in adulthood can be reduced by maternal DHA supplementation.

Effect of pelleted high-oil canola meal from on-farm biodiesel production on rumen fermentation in lactating Holstein dairy cows

<h2>ABSTRACT</h2> Four lactating, ruminally cannulated Holstein cows (DIM ≥50; ≥second lactation) were paired by milk production and used in a switchback design to compare ruminal fermentation response to a diet containing 7% pelleted, high-oil canola meal (HOCM) versus a control diet (CONT). The CONT diet was made of corn silage, alfalfa haylage, wheat straw, and the concentrate. The HOCM diet contained the same ingredients as the CONT except sunflower seeds and a portion of soybean meal were replaced with pelleted, high-oil canola meal in the concentrate. The canola pellet contained 16.6% crude fat and 37.3% CP. Diets were formulated to be isonitrogenous and isocaloric. The last day of each 21-d switchback period was used to collect ruminal fermentation data. Ruminal fluid samples were collected through the cannula at 0, 2, 4, 6, and 8 h after feeding. Cows were housed in a tie-stall barn, and diets were delivered as a TMR once daily at 1000 h to achieve ad libitum intake (5% orts). Daily DMI and milk production data were recorded during the entire feeding period. There were no differences (P ≥ 0.29) between DMI, milk yield, and efficiency of milk production of cows fed the HOCM and the CONT diets. Ruminal acetate, butyrate, total VFA, and pH were not affected (P ≥ 0.37) by diets. Ruminal propionate tended to be greater (P = 0.09) in cows fed HOCM. Adding 7% of pelleted, high-oil canola meal to lactating Holstein dairy cow diets did not adversely affect ruminal fermentation.

Twenty-four hour energy expenditure and substrate oxidation before and after 6 months' ad libitum intake of a diet rich in simple or complex carbohydrates or a habitual diet.

To investigate 24 h energy expenditure (24 h EE) and substrate oxidations in overweight and obese subjects before and after 6 months' ad libitum intake of a low-fat, high-simple carbohydrate diet (SCHO), a low-fat, high-complex carbohydrate diet (CCHO), or a habitual control diet (CD). Twenty-four healthy overweight and obese subjects (11 males and 13 females; body mass index 30.7+/-0.6 kg/m(2); age 42.2+/-1.8 y). Twenty-four hour EE, substrate oxidation rates and spontaneous physical activity (SPA) measured in a respiration chamber, and food intake. After the intervention no differences were seen in 24 h EE, postprandial thermogenesis, basal metabolic rate or SPA. Carbohydrate oxidation, adjusted for energy balance, increased on both carbohydrate-rich diets (SCHO 13.0%, CCHO 11.5%) and decreased on the CD diet (6.5%); however, the changes were not significantly different between diets. The opposite pattern was seen for fat oxidation, which increased by 2.9% on the CD diet and decreased by 17.1 and 25.6% on the SCHO and CCHO, respectively. The changes only differed between the CD and CCHO diet (P=0.03). Six months' ad libitum intake of a diet rich in simple or complex carbohydrates or a habitual diet induced a shift in the oxidation pattern to closely reflect the diet composition in overweight and obese subjects. No differences between diets were seen in 24 h EE.