The effect of using argon and helium shielding gas on weld quality, defect formation and microstructure of laser welded aluminium alloy 2024-T3 was investigated. Full penetration autogenous welds were made at a constant laser power of 4.9kW using a continuous-wave (CW) fibre laser at travel speeds of 3.0–5.0m/min and focal positions of +4 to −4mm. To investigate this effect, a comparison was made between Ar and He by examining the weld quality in terms of the face and root weld width, the weld width ratio; and the presence of welding defects including undercut, underfill, reinforcement, porosity and crack. Optical metallography, energy-dispersive X-ray spectroscopy and micro-hardness indentation testing on weld cross-sections were used to identify how the chemical and physical properties of the shielding gases and the characteristics of the fibre laser affect the overall weld geometry. Based on the results, it was believed that relatively small influence of ionisation on fibre laser induced plume enhanced the welding process stability and lowered the threshold power density for keyhole formation. Both Ar and He shielding gases could therefore, be used effectively to produce good quality welds. However, at the lowest speed and also at the maximum focal position, higher ionisation potential and thermal conductivity of helium resulted in an excessive weld width when He was used even though, the overall weld quality was better than that with Ar.