Continuous Stress Research Articles

Isolation, Culture, and Analysis of Zebrafish Myofibers and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis.

Adult skeletal musculature experiences continuous physical stress, and hence requires maintenance and repair to ensure its continued efficient functioning. The population of resident muscle stem cells (MuSCs), termed satellite cells, resides beneath the basal lamina of adult myofibers, contributing to both muscle hypertrophy and regeneration. Upon exposure to activating stimuli, MuSCs proliferate to generate new myoblasts that differentiate and fuse to regenerate or grow myofibers. Moreover, many teleost fish undergo continuous growth throughout life, requiring continual nuclear recruitment from MuSCs to initiate and grow new fibers, a process that contrasts with the determinate growth observed in most amniotes. In this chapter, we describe a method for the isolation, culture, and immunolabeling of adult zebrafish myofibers that permits examination of both myofiber characteristics ex vivo and the MuSC myogenic program in vitro. Morphometric analysis of isolated myofibers is suitable to assess differences among slow and fast muscles or to investigate cellular features such as sarcomeres and neuromuscular junctions. Immunostaining for Pax7, a canonical stemness marker, identifies MuSCs on isolated myofibers for study. Furthermore, the plating of viable myofibers allows MuSC activation and expansion and downstream analysis of their proliferative and differentiative dynamics, thus providing a suitable, parallel alternative to amniote models for the study of vertebrate myogenesis.

Versatile lipoprotein-inspired nanocomposites rescue Alzheimer's cognitive dysfunction by promoting Aβ degradation and lessening oxidative stress.

The accumulation of amyloid-β (Aβ) into senile plaques and the resulting continuous oxidative stress are major pathogenic mechanisms in Alzheimer's disease (AD). In this study, we designed a lipoprotein-inspired nanoparticle to facilitate Aβ clearance and alleviate oxidative stress for the treatment of AD. Lipoprotein-like nanocomposites (RLA-rHDL@ANG) were fabricated by assembling reconstituted high density lipoprotein (rHDL) with an apoE-derived peptide (RLA) with Aβ binding and clearance capabilities, and were subsequently camouflaged using reactive oxygen species (ROS)-sensitive DSPE-TK-mPEG2000 and DSPE-TK-PEG3400-ANG with brain penetration as well as ROS scavenging ability. Immunoelectron microscopy, fluorescence colocalization, and enzyme linked immunosorbent assay, together with a thioflavin-T (ThT) fluorescence quantitative test, showed that RLA-rHDL@ANG possessed the ability of high binding affinity to both Aβ monomers and oligomers, and disintegration of pre-formed Aβ aggregates. ROS level monitoring and transmission electron microscopy (TEM) showed that RLA-rHDL@ANG possessed ROS sensitivity and consumption properties. Transcellular assay and in vivo imaging showed that RLA-rHDL@ANG effectively facilitated blood-brain barrier (BBB) penetration and intracerebral accumulation. It promoted the efficient degradation of Aβ by microglia and neurons through lysosomal transport and elimination approaches. Four-week administration of RLA-rHDL@ANG effectively reduced Aβ deposition, decreased the ROS level and improved cognitive functions in AD mice. These findings indicate that multifunctional RLA-rHDL@ANG may serve as a promising and feasible candidate for managing the progression of AD.

Factors influencing traveler use of transit before, during, and after the COVID-19 pandemic

The COVID-19 pandemic initially caused a significant decrease in travel. Whereas highway travel rebounded quickly, transit use has not fully returned to pre-pandemic levels. This paper discusses the results from an internet survey conducted in May and June of 2022 of 6300 travelers that stated their use of transit before and during the pandemic, and their foreseeable use of transit after the pandemic is over. The survey questionnaire developed in this study focused in identifying factors associated with the stated change in transit use, including average stress level and fear of COVID-19. Approximately 41 % of respondents reduced their use of transit after the pandemic declaration in March 2020 and 45.5 % stated they were less willing to use transit in the future even after the pandemic is over. Respondents who stated a lower use of transit during the pandemic also had higher average stress levels and higher fear of COVID-19. A Random Forest Classification Model and a SHAP Value Plot were used to identify factors relevant to the stated reduced use of transit for travelers who were frequent riders before the pandemic. Household size and annual income, the comfort level of the person when faced with a crowded bus, the fear or risk of contracting COVID-19, along with age and gender characteristics, were among the key factors associated with the stated reduction in transit use. In addition to telecommuting and travel mode shifts caused by the pandemic, transit agencies must overcome the continued stresses and fears that the commuters have related to COVID-19 for ridership to return to pre-pandemic levels.

Fatigue assessment for FPSO hawsers

Floating Production Storage and Offloading Unit (FPSO) is designed to produce, store and transport hydrocarbon products. FPSO's hawsers may be exposed to both extreme and fatigue loads during operations. Hence prediction of their fatigue life is important for operational safety. During some unloading operations, consistent hawser tensions could develop as a result of internal friction in nylon ropes, casing wear and accumulated fatigue damage. Methodology, suggested in this study, may be effectively employed at the vessel design phase, when optimizing vessel parameters, reducing potential FPSO hawser tension fatigue damage. This study aims to contribute to development of novel fatigue assessment approaches, in order to use limited available datasets more effectively. Stresses occurring within FPSO hawsers have been modelled, using actual in situ environmental conditions. Simulated continuous stress time series were used as input for the rainflow counting analysis; the cumulative fatigue damage was then evaluated. Note on experimental validation has been provided.

Changes in sleep patterns and the doom-scrolling (doom-surfing) phenomenon as modifiable risk factors for anxiety due to continuous stress of the COVID-19 pandemic

Changes in sleep patterns and the doom-scrolling (doom-surfing) phenomenon as modifiable risk factors for anxiety due to continuous stress of the COVID-19 pandemic

Identification of Linear Elastic Fracture Mechanics Parameters Through the Atomistic Simulation Based Analysis

The contribution presents atomistic modeling of the proximate crack tip and notch stress fields through molecular dynamics method. The study is aspired to comparing atomistic stress distributions for monocrystalline fcc copper and aluminum with continuous stress fields obtained on the basis of classical continuum fracture mechanics. Atomistic simulations were implemented for pure tensile loadings and mixed (I+II mixed loadings) mode loadings in Large-scale Atomistic/ Molecular Massively Parallel Simulator (LAMMPS). In atomistic computations specimens with central cracks and single edge notches made of crystalline fcc copper and aluminum under mixed mode loadings are considered. The elastic tensor components of the selected crystalline materials are obtained via molecular dynamics simulations and the circumferential distributions of stress fields in the straight away proximity of the crack and notch tips in aluminum and copper are constructed. The main regularities arising from the comparative analysis of the atomistic and continuum approaches are revealed. The major conclusion that can be reached after atomistic modeling is that the stress apportionments given by classical linear fracture mechanics qualitatively and quantitatively describe the molecular dynamics stress distributions.

CKL2 mediates the crosstalk between abscisic acid and brassinosteroid signaling to promote swift growth recovery after stress in Arabidopsis.

Plants must adapt to the constantly changing environment. Adverse environmental conditions trigger various defensive responses, including growth inhibition mediated by phytohormone abscisic acid (ABA). When the stress recedes, plants must transit rapidly from stress defense to growth recovery, but the underlying mechanisms by which plants switch promptly and accurately between stress resistance and growth are poorly understood. Here, using quantitative phosphoproteomics strategy, we discovered that early ABA signaling activates upstream components of brassinosteroid (BR) signaling through CASEIN KINASE 1-LIKE PROTEIN 2 (CKL2). Further investigations showed that CKL2 interacts with and phosphorylates BRASSINOSTEROID INSENSITIVE1 (BRI1), the main BR receptor, to maintain the basal activity of the upstream of BR pathway in plants exposed to continuous stress conditions. When stress recedes, the elevated phosphorylation of BRI1 by CKL2 contributes to the swift reactivation of BR signaling, which results in quick growth recovery. These results suggest that CKL2 plays a critical regulatory role in the rapid switch between growth and stress resistance. Our evidence expands the understanding of how plants modulate stress defense and growth by integrating ABA and BR signaling cascades.

Comparing online cognitive load on mobile versus PC-based devices.

Navigating the web represents a complex cognitive activity that requires effective integration of different stimuli and the correct functioning of numerous cognitive abilities (including attention, perception, and working memory). Despite the potential relevance of the topic, numerous limitations are present throughout the literature about the cognitive load during online activities. The main aim of this study is to investigate cognitive load during comprehension and information-seeking tasks. In particular, we here focus on the comparison of the cognitive load required while performing those tasks using mobile or PC-based devices. This topic has become even more crucial due to the massive adoption of smart working and distance learning during the COVID-19 pandemic. A great effort is nowadays devoted to the detection and quantification of stressful states induced by working and learning activities. Continuous stress and excessive cognitive load are two of the main causes of mental and physical illnesses such as depression or anxiety. Cognitive load was measured through electroencephalography (EEG), acquired via a low-cost wireless EEG headset. Two different tasks were considered: reading comprehension (CO) of online text and online information-seeking (IS). Moreover, two experimental conditions were compared, administering the two tasks using mobile (MB) and desktop (PC) devices. Eleven participants were involved in each experimental condition, MB and PC, performing both the tasks on the same device, for a total of twenty-two people, recruited from students, researchers, and employees of the university. The following two research questions were investigated: Q1: Is there a difference in the cognitive load while performing the comprehension and the information-seeking tasks? Q2: Does the adopted device influence the cognitive load? The results obtained show that the baseline (BL) requires the lower cognitive load in both the conditions, while in IS task, the requirement reaches its highest value, especially using a mobile phone. In general, the power of all the brain wave bands increased in all conditions (MB and PC) during the two tasks (CO and IS), except for alpha, which is usually high in a state of relaxation and low cognitive load. People include website navigation into their daily routines, and for this, it is important to create an interaction that is as easy and barrier-free as possible. An effective design allows a user to focus on interesting information: many website architectures, instead, are an obstacle to be overcome; they impose a high cognitive load and poor user experience. All these aspects draw cognitive resources away from the user's primary task of finding and comprehending the site's information. Having information about how the cognitive load varies based on the device adopted and the considered task can provide useful indicators in this direction. This work suggests that using an EEG low-cost wearable device could be useful to quantify the cognitive load induced, allowing the development of new experiments to analyse these dependencies deeper, and to provide suggestions for better interaction with the web.

Prototype of Instrumented Rock Bolt for Continuous Monitoring of Roof Fall Hazard in Deep Underground Mines

Roof falls are currently one of the most dangerous threats associated with underground mining at great depth. Every occurrence of such an event poses a significant risk to the mining crew and disturbs the continuity of the mining process, which clearly affects the economy of the exploitation process. The development of a reliable monitoring system may significantly reduce the impact of eventual roof failure and will have a positive effect on the sustainability of the extraction process. Within this research study, a prototype of an instrumented rock bolt developed for continuous stress measurement is presented. The procedure of a 4-groove multilevel instrumented rock bolt is described and the calibration process is shown. Then, preliminary results of long-term in situ monitoring are presented. Based on the continuous monitoring of stress distribution within immediate roof strata, it was concluded that the developed instrumented rock bolt provides reliable results and is a very useful device, ensuring the possibility of early warning for miners about increasing roof fall risk.

A protocol for rapid generation cycling (speed breeding) of hemp (Cannabis sativa) for research and agriculture.

Hemp (Cannabis sativa) is a highly versatile crop with a multitude of applications, from textiles, biofuel and building material to high-value food products for consumer markets. Furthermore, non-hallucinogenic cannabinoids like cannabidiol (CBD), which can be extracted from female hemp flowers, are potentially valuable pharmacological compounds. In addition, hemp has high carbon sequestration potential associated with its rapid growth rate. Therefore, the hemp industry is gaining more traction and breeding hemp cultivars adapted to local climate conditions or bred for specific applications is becoming increasingly important. Here, we present a method for the rapid generation cycling (speed breeding) of hemp. The speed breeding protocol makes use of the photoperiod sensitivity of Cannabis. It encompasses vegetative growth of the plants for 2 weeks under continuous light, followed by 4 weeks under short-day conditions, during which flower induction, pollination and seed development proceed, and finally a seed ripening phase under continuous light and water stress. With the protocol described here, a generation time of under 9 weeks (61 days) from seed to seed can be achieved. Furthermore, our method synchronises the flowering time of different hemp cultivars, thus facilitating crosses between cultivars. The extremely short generation time will enable hemp researchers and breeders to perform crosses in a time-efficient way and generate new hemp cultivars with defined genetic characteristics over a short period of time.

Implementation of occupational health and safety (OHS) management system to work stress among health workers during the COVID-19 pandemic at the Daya Regional General Hospital, Makassar

Implementation of Occupational Safety and Health is one form of effort to create a safe workplace to reduce occupational diseases. The continuous work stress faced by health workers during the Covid-19 pandemic is likely to cause stress. The research aims to analyze the application of the OSH management system to work stress in health workers during the Covid-19 pandemic at the Daya Regional General Hospital in Makassar City in 2022. Methods: This research is a quantitative and qualitative (combined) approach. The analysis used was descriptive-analytic analysis with a cross-sectional approach. The sampling technique is a technique with purposive sampling. The sample was 61 nurses and the interviews were conducted with 2 K3 officers, 1 IPCLN officer, and 4 nurse room heads. Results: Of the quantitative study showed that there was a relationship between the implementation of K3RS (p=0.026), work shifts (p=0.027), workload (p=0.017), and years of service (p=0.001) on work stress. The results of the qualitative research show that most health workers have a high workload, long working hours, and standardized work shifts that can affect K3RS implementation and are not optimal. Conclusion: The four variables, namely the implementation of K3RS, workload, work shifts, and years of service had a relationship with work stress in Daya Hospital. His advice is to regularly review and continuously improve SMK3 to improve overall OSH performance and preferably establish a special budget to support the implementation of occupational safety and health at Daya Hospital.

Persistent Inflammation Initiated by TORCH Infections and Dysbiotic Microbiome in Autism Spectrum Disorders: A Prospect for Future Interventions

Autism spectrum disorders (ASD) are a range of neurodevelopmental conditions that are clinically present early in childhood with the symptoms of social withdrawal and repetitive behavior. Despite an extensive research on ASD, no commonly accepted theory on the disease etiology exists. Hence, we reviewed several scientific publications, including reviews, preclinical and clinical investigations, and published hypotheses to analyze various opinions on the nature and cause of the disorder. Many studies suggest that infections and inflammation during pregnancy play a significant role in genetic and epigenetic changes in the developing fetus, resulting in an autistic phenotype in a child. Still, there is a lack of comprehensive literature about the multitude of autism inducing factors. Therefore, this article reviews and discusses available scientific evidence on the roles of viral, bacterial, fungal, and parasitic infections, overactivation of the immune system, and intestinal microflora in the pathogenesis and clinical manifestation of ASD. The overview of the scientific publications, including our own studies, suggests that TORCH infections, imbalanced microbiome, and persistent inflammation are significantly associated with the disruption of the social domain in ASD children. The ASD-related changes begin prenatally as maternal-to-fetal immune activation triggered by infection. It results in continuous low-grade inflammation and oxidative stress in a fetus, causing germline and somatic genetic changes in the developing brain and the establishment of the dysregulated immune system. These changes and dysregulations result in central and peripheral nervous systems dysfunctions as well as other comorbid conditions found in autistic children.

Education Advisors’ Experiences of Stress and Coping in the Virtual Working Environment

During the CoVID-19 pandemic, most educational advisors were forced to work from home. Continuous stress may lay negative impacts on educators’ work performance and psychological state (Hughes et al., 2019). However, there are no studies on stress whilst working virtually for educational advisors during the CoVID-19. Therefore, understanding stress in the lockdown environment is important to support them in coping. A qualitative study using semi-structured interviews was conducted. Following snowball sampling, 12 interviews were conducted (Male=7, Female=5, Mage=31.74) from the UK and China. The six phases of thematic analysis were employed to analyze the data. The interviews identified the sources of educational advisors’ stress from colleagues, clients, families and personal environment that laid negative consequences on their work and life, such as ineffective work, anxiety and low mood, etc. A series of problem-focused and emotion-focused coping strategies, such as boundary management, were raised for them to cope. Underpinned by Lazarus and Folkman’s (1984) Transactional Model of Stress and Coping, the findings highlighted the educational advisors’ stress experiences and raised practical coping strategies at personal, organizational and family levels to defend against their stresses whilst working virtually.

Expression of GPR-68 in Human Corneal and Conjunctival Epithelium. Possible indicator and mediator of attrition associated inflammation at the ocular surface

Attrition and osmotic stress have been identified as major forces in the pathophysiology of dry eye. Impaired tolerance to mechano-transduction in the presence of insufficient lubrication has been associated with disturbances of ocular surface homeostasis and encouragement of inflammatory reactions, challenging the usual regulatory coping mechanisms. In spite of the probable link between enhanced attrition and secondary inflammation, the key mediators driving the vicious cycle of severe dry eye disease have not yet been identified. The goal of this study was therefore to investigate human corneal and conjunctival epithelium for the presence of the G protein-coupled receptor GPR-68. This protein had most recently been shown to be not only chemically activated but also mechanically, possibly through attrition. De-identified sections of human cornea and conjunctiva were stained for the presence of G protein-coupled receptor 68 with specific antibodies using immunohistochemical methods. Results Specific staining for G-protein-coupledreceptor 68 (GPR68) was observed in all samples of the cornea throughout the epithelial layers of the corneal epithelium, most prominently in the area of the wing cells and the basement membrane. Even in the conjunctiva, specific staining for GPR-68 was found. The detection of G protein-coupled receptor GPR-68 in human corneal and conjunctival epithelium raises the question of its function and purpose. The mechanical activation of GPR68 in situations with enhanced friction and attrition could modify various cellular functions and possibly jeopardize normal inflammatory homeostasis at the ocular surface. Accordingly, decreased lubrication in dry eye disease could result in activation of GPR-68. This could lead to secondary inflammation, initially in the epithelium and surrounding stroma. Continuous mechanical stress could result in chronic inflammation, also reaching deeper structures of the cornea, possibly making GPR-68 an important actor in the vicious cycle of dry eye disease. G protein-coupled receptor GPR-68, sensitive to flow and mechanic stimulation, is present in the human corneal epithelium and conjunctiva. Decreased lubrication and increased attrition, accompanied by sensations typical for dry eye, might lead to local inflammation. It is possible that subtle signs of conjunctival, and later corneal, surface damage in the context of these sensations could be a better indicator of the need for and success of therapy than the clinical signs of dry eye disease alone, at least in the early stages of the disease. Inhibition of G protein-coupled receptor GPR-68 could represent a new strategy in the treatment of dry eye disease.

Citrullinated glucose-regulated protein 78 is a candidate target for melanoma immunotherapy.

Post translational modification of proteins plays a significant role in immune recognition. In particular the modification of arginine to citrulline which is mediated by PAD enzymes is increased during cellular stress (autophagy) which permits the presentation of modified epitopes upon MHC class II molecules for recognition by CD4 T cells. Citrullination also occurs in tumour cells as a result of continuous environmental stresses and increased autophagy. We have shown in animal models the efficient stimulation of citrullinated epitope specific CD4 T cells resulting in dramatic elimination/regression of tumours. The ER chaperone glucose-regulated protein 78 (GRP78) is known to also be required for stress-induced autophagy and is directly linked to autophagosome formation. GRP78 is known to be highly expressed by many tumour types. In this study we investigate the potential of targeting citrullinated GRP78 for cancer therapy. A citrullinated GRP78 specific antibody was used to assess citrullinated GRP78 expression in murine and human tumour cells by flow cytometry. Five peptides were selected and used to vaccinate HLA transgenic mice and immune responses were characterised by ex vivo cytokine ELISpot assay. T cell repertoire in humans was assessed through proliferation assays and cytokine ELISpot assay. Citrullinated peptide was identified in murine B16 melanoma by mass spectrometry and the peptide vaccine was assessed for tumour therapy in a mouse melanoma model. We show the identification CD4 T cell responses to one citrullinated GRP78 epitope that are restricted through HLA DP*0401 and HLA-DR*0101 alleles. This peptide is detected by mass spectrometry in B16 melanoma grown in vivo and citrulline specific CD4 responses to two peptides spanning this epitope mediate efficient therapy of established B16 melanoma tumours in HHDII/DP4 (p<0.0001) transgenic mouse model. Finally, we demonstrate the existence of a repertoire of responses to the citrullinated GRP78 peptide in healthy individuals (p=0.0023) with 13/17 (76%) individuals showing a response to this peptide. We propose that citrullinated GRP78 is a candidate tumour antigen and vaccination against citrullinated GRP78 may provide a promising tumour therapy approach.

Structural Optimization of a Circular Symmetric Threaded Connection System Based on the Effect of the Upper Stabbing Flank Corner Radius

In order to boost shale gas drilling efficiency, oil and gas exploration and drilling distances have risen along with the number of horizontal definitions and directions. As a result, the joints of oil wells and core gas pipes are under continual stress and compression, internal and external pressures, and extreme bending stresses. This will negatively impact the drilling system’s sustainability and the performance requirements are therefore reinforced. Good thread design is crucial because premium threaded connections are typically employed in joints, and the construction of reliable premium connections to external limitations is closely related to the choice of pertinent parameters as optimal values. In this article, new premium connection systems will be created and constructed that can show good vibration and durability under such stressful conditions. The amount of von Mises stress that is received from the threaded connection depends on how each parameter is changed. In particular, the values of the “upper stabbing flank corner radius” were used to examine the von Mises stress variations in the entire structure. An optimized design for the premium connection system is proposed with the best value of upper stabbing flank corner radius according to minimized stress on the teeth of the premium connection against compressive forces. The study’s findings suggest that it is feasible to create a premium connection that can lower stress following the genuine demand of the industry.

Layerwise theories for composite beams with continuous and discontinuous stresses

Due to the unique characteristics of composite materials, the study of composite beams is far more complex than the study of homogeneous beams. The finite element method has proven to be a powerful approach to analyze composites subjected to the most distinctive situations. In the present work, two element solutions using cubic polynomials are considered: with continuous stresses and with discontinuous stresses along the transverse direction. Both converge to the analytical solution as the number of elements increase, i.e. with a finer mesh. Besides satisfying the boundary conditions at the surfaces and interfaces, the first solution gives better outcomes close to the center of the beam. On the other hand, the second solution gives better outcomes close to the borders of the beam, but it has a larger number of nodal parameters. The results are compared to a zig-zag element solution which has a number of nodal parameters independent of the number of layers. An element based on the Reissner mixed variational theorem is also included for additional comparisons. It is concluded that the cubic polynomials used to expand the cross section functions, must be different in each layer in order to achieve a reasonable agreement between the analytical and the calculated transverse normal stress.

Dynamics of accumulation of phenolic compounds, anthocyanins, and photosynthetic pigments in the vegetative organs of the wheat plants under continuous drought stress

Dynamics of accumulation of phenolic compounds, anthocyanins, and photosynthetic pigments in the vegetative organs of the wheat plants under continuous drought stress

An Analytical Model for Water Inflow into a Karst Tunnel in Vuggy and Fractured Porous Rock Aquifers

Vugs and fractures are the main channels within porous rock aquifers and play a dominant role in the water inrush of the karst tunnel. In this paper, a mathematical model of the coupled free-fluid seepage-fluid with second-order accuracy was established to analyze the flow field characteristics of vuggy and fractured porous rock aquifers. The Navier–Stokes equation was used to govern the free fluid flow in vugs and fractures, while the Darcy–Brinkman equation was employed to describe the seepage fluid motion in the porous rock matrix. The analytical solutions for the flow velocity and water discharge were derived under the requirement of continuous velocity and shear stress at the interface between free and seepage fluids. These solutions can be successfully reduced to those of the classical Poiseuille’s law, assuming that the rock matrix is impervious to water. The numerical, experimental, and analytical results were compared to validate the present analytical model, and the results of the analytical model showed greater accuracy compared with the classical Poiseuille’s law and cubic law. Furthermore, the water inrush of the Xiangpingshan karst tunnel was selected as a real engineering case. Perforated bricks were used to stack a vuggy and fractured porous physical tunnel model (1:100) with the same hydraulic conductivity as the Xiangpingshan tunnel. The relative errors between current theoretical and physical tests obtained for the water inflow discharges are within 18%. The current model can provide a reference for predicting the water inflow of karst tunnels in vuggy and fractured porous rock aquifers.

Effect of distribution characters of ferrite/bainite on deformation compatibility in dual-phase pipeline steel: Experimental and numerical study

The morphology distribution of microstructure has an important influence on the match of the strength and plasticity of dual-phase pipeline steel. Bainite and PF dual-phase steels with layered and network morphology were obtained by combining the Thermomechanical Control Process (TMCP) and inter-critical isothermal heat treatment. A novel experimental-numerical methodology studied the effect of phase distribution characters on the deformability of PF and bainite dual-phase pipeline steel. The plastic damage features and microscopic strain and stress distribution in both network and layered microstructure were clarified. The obvious differences in the microstructure evolution for both samples were captured by a quasi-situ tensile test. The PF phase in the network morphology sample was believed the main deformation phase, but the deformation of the PF phase in the layered morphology sample was restrained. Two three-dimensional (3D) crystal plasticity finite element models (CPFEMs) were established based on the electron backscatter diffraction (EBSD) data. The simulation results indicated that the strain field in layered microstructure was more uniform than that in network microstructure, and the continuous internal stress relaxation (ISR) in the bainite phase led to plastic damage at low tensile strain level; The strain field in network microstructure mainly existed in the PF phase, the deformation of the PF phase can be fully utilized. When changing the tensile x-direction of the layered microstructure to the z-direction, the strain field showed an obvious anisotropy, and loading in the z-direction increased the risk of interlaminar crack formation. • Quasi-situ tensile test was used to study the deformation behavior • Strain/stress distribution characteristics were clarified using CPFEM method • Uniform strain distribution and ISR in layered microstructure decreased its plasticity • z-axis loading increased the risk of interlaminar crack for layered microstructure