Experimental Context Research Articles

American Capitalism and the Decline of Debtors’ Prison in the 19th Century

This paper examines the dynamics of capitalism and the penitentiary system through an analysis of the decline of debt imprisonment in 19th-century United States. It argues that class transformations in the early to mid-1800s created a context for social experiments with new forms of punishment. The penitentiary systems that formed developed logics that contingently fit with the rising mode of production. The decline of debt imprisonment, in this context, was much in part the result of transformations in class power and class structure.

Thermal Conductivity of Polymers: A Simple Matter Where Complexity Matters.

Thermal conductivity coefficient κ measures the ability of a material to conduct a heat current. In particular, κ is an important property that often dictates the usefulness of a material over a wide range of environmental conditions. For example, while a low κ is desirable for the thermoelectric applications, a large κ is needed when a material is used under the high temperature conditions. These materials range from common crystals to commodity amorphous polymers. The latter is of particular importance because of their use in designing light weight high performance functional materials. In this context, however, one of the major limitations of the amorphous polymers is their low κ, reaching a maximum value of ≈0.4 W/Km that is 2-3 orders of magnitude smaller than the standard crystals. Moreover, when energy is predominantly transferred through the bonded connections, κ ⩾ 100 W/Km. Recently, extensive efforts have been devoted to attain a tunability in κ via macromolecular engineering. In this work, an overview of the recent results on the κ behavior in polymers and polymeric solids is presented. In particular, computational and theoretical results are discussed within the context of complimentary experiments. Future directions are also highlighted.

Assessing the biological effects of boron neutron capture therapy through cellular DNA damage repair model.

Boron neutron capture therapy (BNCT) is a targeted radiotherapy that relies on the 10B (n, α) 7Li reaction, which produces secondary particles with high linear energy transfer (LET), leading to a high relative biological effectiveness (RBE) in tumors. The biological effectiveness of BNCT is influenced by factors such as boron distribution and concentration, necessitating improved methods for assessing its radiobiological effects and clarifying the sensitivity of the differences in different factors to the biological effects. This paper introduces a method to evaluate the biological effects of BNCT using the cellular repair model. This method aims to overcome some of the limitations of current evaluation approaches. The primary goal is to provide guidance for clinical treatments and the development of boron drugs, as well as to investigate the impact of the synergistic effects of mixed radiation fields in BNCT on treatment outcomes. The approach involves three key steps: first, extending the radial energy deposition distribution of BNCT secondary particles using Geant4-DNA. This allows for the calculation of initial DNA double-strand breaks (DSBs) distributions for a given absorbed dose. Next, the obtained initial DSB distributions are used for DNA damage repair simulations to generate cell survival curves, then thereby quantifying RBE and compound biological effectiveness (CBE). The study also explores the synergistic effects of the mixed radiation fields in BNCT on assessing biological effects were also explored in depth. The results showed that the RBE of boronophenylalanine (BPA) and sodium borocaptate (BSH) drugs at cell survival fraction 0.01 was 2.50 and 2.15, respectively. The CBE of the boron dose component was 3.60 and 0.73, respectively, and the RBE of the proton component was 3.21, demonstrating that BPA has a significantly higher biological impact than BSH due to superior cellular permeability. The proton dose significance in BNCT treatment is also underscored, necessitating consideration in both experimental and clinical contexts. The study demonstrates that synergistic effects between disparate radiation fields lead to increased misrepairs and enhanced biological impact. Additionally, the biological effect diminishes with rising boron concentration, emphasizing the need to account for intercellular DNA damage heterogeneity. This methodology offers valuable insights for the development of new boron compounds and precise assessment of bio-weighted doses in clinical settings and can be adapted to other therapeutic modalities.

The exocyst in context.

The exocyst is a hetero-octameric complex involved in the exocytosis arm of cellular trafficking. Specifically, it tethers secretory vesicles to the plasma membrane, but it is also a main convergence point for many players of exocytosis: regulatory proteins, motor proteins, lipids and Soluble N-ethylmaleimide-sensitive factor Attachment Protein Receptor (SNARE) proteins are all connected physically by the exocyst. Despite extensive knowledge about its structure and interactions, the exocyst remains an enigma precisely because of its increasingly broad and flexible role across the exocytosis process. To solve the molecular mechanism of such a multi-tasking complex, dynamical structures with self, other proteins, and environment should be described. And to do this, interrogation within contexts increasingly close to native conditions is needed. Here we provide a perspective on how different experimental contexts have been used to study the exocyst, and those that could be used in the future. This review describes the structural breakthroughs on the isolated in vitro exocyst, followed by the use of membrane reconstitution assays for revealing in vitro exocyst functionality. Next, it moves to in situ cell contexts, reviewing imaging techniques that have been, and that ideally could be, used to look for near-native structure and organization dynamics. Finally, it looks at the exocyst structure in situ within evolutionary contexts, and the potential of structure prediction therein. From in vitro, to in situ, cross-context investigation of exocyst structure has begun, and will be critical for functional mechanism elucidation.

Kappa-opioid receptor blockade in the inferior colliculus of prey threatened by pit vipers decreases anxiety and panic-like behaviour.

The dorsal midbrain comprises dorsal columns of the periaqueductal grey matter and corpora quadrigemina. These structures are rich in beta-endorphinergic and leu-enkephalinergic neurons and receive GABAergic inputs from substantia nigra pars reticulata. Although the inferior colliculus (IC) is mainly involved in the acoustic pathways, the electrical and chemical stimulation of central and pericentral nuclei of the IC elicits a vigorous defensive behaviour. The defensive immobility and escape elicited by IC activation is commonly related to panic-like emotional states. To investigate the role of κ-opioid receptor of the IC in the antiaversive effects of endogenous opioid receptor blockade in a dangerous situation, male Wistar rats were pretreated in the IC with the κ-opioid receptor-selective antagonist nor-binaltorphimine at different concentrations and submitted to the non-enriched polygonal arena for a snake panic test in the presence of a rattlesnake and, after 24 h, prey were resubmitted to the experimental context. The snakes elicited in prey a set of antipredatory behaviours, such as the anxiety-like responses of defensive attention and risk assessment, and the panic-like reactions of defensive immobility and either escape or active avoidance during the elaboration of unconditioned and conditioned fear-related responses. Pretreatment of the IC with microinjections of nor-binaltorphimine at higher concentrations significantly decreased the frequency and duration of both anxiety- and panic-attack-like behaviours. These findings suggest that κ-opioid receptor blockade in the IC causes anxiolytic- and panicolytic-like responses in threatening conditions, and that kappa-opioid receptor-selective antagonists can be a putative coadjutant treatment for panic syndrome treatment.

AI for Cloud Cost Management: Predictive and Prescriptive Analytics

With reference to the previous information and literature review, the purpose of this research paper is to evaluate the impact of multithreading concerning computer resource utilization and performance by undertaking and comparing various crucial multithreading techniques, including work stealing, fork join, and the use of the thread pool control. In an experimental context, benchmark applications that can be characteristic of several domains were evaluated in a controlled multicore computing platform. Time, CPU occupation, memory usage and throughputs were determined systematically and compared according to the various workloads. The results highlight the superiority of the work-steing algorithm in keeping the execution time and CPU usage low compared to the rest as a sign of its efficiency in managing work loads. Further, the memory usage and the throughput statistics showed the degree of inefficiency of each algorithm and performance penalty. Interviews with developers elaborated on more practical issues of multithreading as the generally rigid nature of the problems suggested that optimal solutions called for adaptive measures in practice. Based on the results achieved in this research, developers and researchers can increase their knowledge about available multithreading algorithms and make suggestions to choose and apply them with high efficiency and low resource consumption.

Finding food: how generalist predators use contact-chemosensory information to guide prey preferences.

Understanding the processes that guide carnivores in finding and selecting prey is a fundamental, unresolved challenge in sensory biology. To our knowledge, no published work has yet revealed the complete structural identities of compounds that cue preferences by generalist predators for different prey species. With this research imperative in mind, we determined the chemistry driving consumer preferences for live intact prey using two generalist predatory species (sea stars, Pisaster ochraceus; whelks, Acanthinucella spirata), along with two foundation prey species (mussels, Mytilus californianus; barnacles, Balanus glandula), inhabiting rocky, wave-swept shores. Each prey species is known to secrete either a 29.6 kDa (named 'KEYSTONEin') or a 199.6 kDa (named 'MULTIFUNCin') glycoprotein as a contact-chemical cue. Here, experimental manipulations utilized faux prey consisting of cleaned barnacle or mussel shells infused with KEYSTONEin, MULTIFUNCin or seawater (control) gels. Whelks exhibited a strong penchant for MULTIFUNCin over KEYSTONEin, irrespective of shell type. In contrast, sea stars generally preferred KEYSTONEin over MULTIFUNCin, but this preference shifted depending on the experimental context in which they encountered physical (shell) and chemical (glycoprotein) stimuli. This study ultimately demonstrates clear and contrasting chemical preferences between sea stars and whelks. It highlights the importance of experimental setting in determining chemical preferences. Finally, it shows that prey preferences by these predators hinge only on one or two contact-protein cues, without the need for quality coding via fluid-borne compounds, low-molecular-weight substances or mixture blends.

Numerical methods for scalar field dark energy in tabletop experiments and Lunar Laser Ranging

Numerous tabletop experiments have been dedicated to exploring the manifestations of screened scalar field dark energy, such as symmetron or chameleon fields. Precise theoretical predictions require simulating field configurations within the respective experiments. This paper focuses onto the less-explored environment-dependent dilaton field, which emerges in the strong coupling limit of string theory. Due to its exponential self-coupling, this field can exhibit significantly steeper slopes compared to symmetron and chameleon fields, and the equations of motion can be challenging to solve with standard machine precision. We present the first exact solution for the geometry of a vacuum region between two infinitely extended parallel plates. This solution serves as a benchmark for testing the accuracy of numerical solvers. By reparametrizing the model and transforming the equations of motion, we show how to make the model computable across the entire experimentally accessible parameter space. To simulate the dilaton field in one- and two-mirror geometries, as well as spherical configurations, we introduce a non-uniform finite difference method. Additionally, we provide an algorithm for solving the stationary Schrödinger equation for a fermion in one dimension in the presence of a dilaton field. The algorithms developed here are not limited to the dilaton field, but can be applied to similar scalar-tensor theories as well. We demonstrate such applications at hand of the chameleon and symmetron field. Our computational tools have practical applications in a variety of experimental contexts, including gravity resonance spectroscopy (q Bounce), Lunar Laser Ranging (LLR), and the upcoming Casimir and Non-Newtonian Force Experiment (cannex). A Mathematica implementation of all algorithms is provided.

Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits.

We present an enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits. Best-in-class vectors were curated for accessing major striatal neuron populations including medium spiny neurons (MSNs), direct and indirect pathway MSNs, as well as Sst-Chodl, Pvalb-Pthlh, and cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes of virus delivery, and with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable cell type specific labeling under different experimental contexts. We demonstrate direct pathway circuit-selective optogenetic perturbation of behavior and multiplex labeling of striatal interneuron types for targeted analysis of cellular features. Lastly, we show conserved in vivo activity for exemplary MSN enhancers in rat and macaque. This collection of striatal enhancer AAVs offers greater versatility compared to available transgenic lines and can readily be applied for cell type and circuit studies in diverse mammalian species beyond the mouse model.

Long-term fertilization reshaped the accumulation of plant- and microbially-derived carbon by regulating biotic and abiotic factors in acidic paddy soil

Fertilization exerts a profound influence on the formation, turnover and stabilization of soil organic carbon (SOC). However, how long-term fertilization regulates the contribution of plant residues and microbial necromass to SOC sequestration in acidic paddy soil remains unclear. This study aimed to elucidate these mechanisms by determining plant and microbial biomarkers, i.e., lignin phenols and amino sugars, as well as carbon degradation genes in both topsoil (0–15 cm) and subsoil (15–30 cm) within the context of a 10-year paddy field experiment. We investigated four fertilization regimes: no fertilizers (control), chemical fertilizers applied alone (NPK), NPK combined with cattle manure (NPKM), and NPK combined with rice straw (NPKS). Compared to the control, integrated organic-inorganic fertilization increased SOC by 15–21 % and 14–19 % in the topsoil and subsoil, respectively. Specifically, NPKM and NPKS did not alter the contribution of total lignin phenols to SOC in the topsoil but increased that in the subsoil by 63 % and 26 %, respectively. SOC accumulation exhibited a positive correlation with the concentration of total lignin phenols but a negative correlation with the acid to aldehyde ratio of vanillyl, indicating selective preservation of vanillyls. However, NPKM and NPKS decreased the contribution of total microbial residual carbon (MRC) to SOC in the topsoil (13–16 %) but didn't alter that in the subsoil. The decreased MRC contribution of topsoil was mainly reflected in fungal MRC, implying that fungi played a pivotal role in regulating topsoil carbon accumulation. Correlation analyses indicated that soil biotic and abiotic factors jointly affected the sequestration of plant lignin phenols and microbial necromass, and topsoil carbon accumulated with the abundance ratio of hydrolytic to oxidative enzyme-coding genes increasing (r = 0.62, p < 0.05). Combined with Partial Least Squares Path Model, our study revealed that the accumulation of organic carbon in the topsoil was more influenced by plant-derived carbon, whereas in the subsoil, it was co-affected by both plant- and microbially-derived carbon.

Near-Infrared Ratiometric Hemicyanine Fluorescent Probes for Monitoring Mitochondrial pH Dynamics in Live Cells during Oxidative Stress and Hypoxia.

Novel near-infrared ratiometric molecules (probes A and B) produced by linking formyl-functionalized xanthene and methoxybenzene moieties, respectively, onto a xanthene-hemicyanine framework are detailed. Probe A exhibited a primary absorption peak at 780 nm and a shoulder peak at 730 nm and exhibited fluorescence at 740 nm↓ (signifies a downward shift in intensity upon acidification) in a pH 9.3 buffer and 780 nm↑ at pH 2.8 under excitation at 700 nm. Probe B featured absorptions at 618 and 668 nm at pH 3.2 and at 717 nm at pH 8.6, and fluorescence at 693 nm↑ at pH 3.2 and at 739 nm↓ at pH 8.6, in mostly the red to near-IR region. The ratiometric changes in the intensity of the fluorescent absorptions were reversed between A and B upon acidification as indicated by the arrows. Theoretical calculations confirmed that there were slight changes in conformation between probes and the protonated molecules, suggesting that the changes in emission spectra were due mostly to conjugation effects. Calculations at the APFD/6-311+g(d,p) level with a solvent described by the polarizable continuum model resulted in pK a values for A at 6.33 and B at 6.41, in good agreement with the experimentally determined value of 6.97 and an average of 6.40, respectively. The versatilities of the probes were demonstrated in various experimental contexts, including the effective detection of mitochondrial pH fluctuations. Live cell experiments involving exposure to different pH buffers in the presence of H+ ionophores, monitoring mitophagy processes during cell starvation, studying hypoxia induced by CoCl2 treatment, and investigating responses to various oxidative stresses are detailed. Our findings highlight the potential of attaching xanthene and methoxybenzaldehyde groups onto xanthene-hemicyanine structures as versatile tools for monitoring pH changes in a variety of cellular environments and processes.

Context-specific variation in life history traits and behavior of Aedes aegypti mosquitoes.

Aedes aegypti, the vector for dengue, chikungunya, yellow fever, and Zika, poses a growing global epidemiological risk. Despite extensive research on Ae. aegypti's life history traits and behavior, critical knowledge gaps persist, particularly in integrating these findings across varied experimental contexts. The plasticity of Ae. aegypti's traits throughout its life cycle allows dynamic responses to environmental changes, yet understanding these variations within heterogeneous study designs remains challenging. A critical aspect often overlooked is the impact of using lab-adapted lines of Ae. aegypti, which may have evolved under laboratory conditions, potentially altering their life history traits and behavioral responses compared to wild populations. Therefore, incorporating field-derived populations in experimental designs is essential to capture the natural variability and adaptability of Ae. aegypti. The relationship between larval growing conditions and adult traits and behavior is significantly influenced by the specific context in which mosquitoes are studied. Laboratory conditions may not replicate the ecological complexities faced by wild populations, leading to discrepancies in observed traits and behavior. These discrepancies highlight the need for ecologically relevant experimental conditions, allowing mosquito traits and behavior to reflect field distributions. One effective approach is semi-field studies involving field-collected mosquitoes housed for fewer generations in the lab under ecologically relevant conditions. This growing trend provides researchers with the desired control over experimental conditions while maintaining the genetic diversity of field populations. By focusing on variations in life history traits and behavioral plasticity within these varied contexts, this review highlights the intricate relationship between larval growing conditions and adult traits and behavior. It underscores the significance of transstadial effects and the necessity of adopting study designs and reporting practices that acknowledge plasticity in adult traits and behavior, considering variations due to larval rearing conditions. Embracing such approaches paves the way for a comprehensive understanding of contextual variations in mosquito life history traits and behavior. This integrated perspective enables the synthesis of research findings across laboratory, semi-field, and field-based investigations, which is crucial for devising targeted intervention strategies tailored to specific ecological contexts to combat the health threat posed by this formidable disease vector effectively.

On testimony in scenarios with Wigner and Friend

The paper constructs a semi-formal language suited to the analysis of Wigner’s Friend scenarios: it represents an epistemic notion of rational beliefs and perspectives, to accommodate the insights of perspectival interpretations of quantum mechanics. The language is then used to analyze a paradox put forward by Frauchiger and Renner (Nat Commun, 9(1):3711, 2018). Their argument is presented as a semi-formal derivation with specified rules of reasoning. These rules bear an affinity to some of the cherished tenets of epistemology and we argue that they are valid (one universally, and the other in experimental contexts). Since our proof is a reductio, it leaves a choice which premises are responsible for a contradiction. Our first choice is a step that appears incorrect from the point of view of the universal unitary evolution as well as the view that every measurement induces a collapse of a measured system’s state. Our second choice, brought to view by the paper’s attention to perspectives and epistemology, points to a step reporting the transmission of beliefs (testimony) about measurement results. We argue that testimony is not licensed by quantum mechanical formalism; we discuss some recent attempts to save the cogency of testimony in the context of quantum measurements.

T-CLEARE: a pilot community-driven tissue clearing protocol repository.

Selecting and implementing a tissue clearing protocol is challenging. Established more than 100years ago, tissue clearing is still a rapidly evolving field of research. There are currently many published protocols to choose from, and each performs better or worse across a range of key evaluation factors (e.g., speed, cost, tissue stability, fluorescence quenching). Additionally, tissue clearing protocols are often optimized for specific experimental contexts, and applying an existing protocol to a new problem can require a lengthy period of adaptation by trial and error. Although the primary literature and review articles provide a useful starting point for optimization, there is growing recognition that results can vary dramatically with changes to tissue type or antibody used. To help address this issue, we have developed a novel, freely available repository of tissue clearing protocols named T-CLEARE (Tissue CLEAring protocol REpository; https://doryworkspace.org/doryviz). T-CLEARE incorporates community responses to an open survey designed to capture details not commonly found in the scientific literature, including modifications to published protocols required for specific use cases and instances when tissue clearing protocols did not perform well (negative results). The goal of T-CLEARE is to help the community share evaluations and modifications of tissue clearing protocols for various tissue types and potentially identify best-in-class methods for a given application.

Puerarin ameliorates colitis by direct suppression of macrophage M1 polarization in DSS mice

BackgroundUlcerative colitis (UC) is a type of inflammatory bowel disease primarily affecting the colon and rectum. The clinical symptoms of UC include persistent diarrhea, abdominal pain, and rectal bleeding, with chronic inflammation often limited to the mucosal layer of the colon. Macrophages play a significant role in the pathogenesis of UC in response to the presence of gut microbiota. Puerarin is an active compound derived from the root of pueraria lobata, a traditional Chinese herbal medicine, and exhibits potent anti-inflammatory properties in various diseases and disease models including UC-like colitis in mice. However, how the molecule achieves its therapeutic effect in colitis by re-polarizing macrophages remains poorly understood. PurposeUtilizing in vivo and in vitro experimental methods along with multi-omics analysis, we aimed to elucidate the potential mechanism by which puerarin targets macrophages to treat colitis. MethodsThe inflammation induced by DSS was assessed both locally in the gut and systemically, and the anti-inflammatory effect of puerarin was evaluated using molecular and histological assays such as H&E staining, qPCR, ELISA, Western blot, and immunofluorescence. Intestinal permeability parameters were measured by in vivo imaging, immunofluorescence, Western blot, qPCR, and PAS staining. The central role of macrophages in colitis was investigated through macrophage depletion/infusion using cytological methods. The direct effects of puerarin on the macrophages were examined by CCK8, flow cytometry, and qPCR in vitro. Additionally, 16S rRNA sequencing and metabolomics analysis of gut contents were conducted. Identification of key pathogenic flora was facilitated by a trans-omic approach and validated both in vitro and in vivo. ResultsPuerarin exerted a direct and robust suppression of M1-like polarization of macrophages in vitro, which was sufficient to confer therapeutic benefits in terms of colonic lesions and systemic inflammation in DSS mice. Puerarin also reduced the abundance of Akkermansia muciniphila in the gut, which was significantly upregulated in DSS mice in our experimental context. Further study demonstrated that puerarin effectively suppressed M1-like macrophage activation induced by Akkermansia muciniphila secreted protein Amuc_2172, thereby altering the pathology in the DSS model. ConclusionOur data suggest that the pathogenesis of DSS colitis is mediated by host cellular responses to toxic foreign molecules and the gut microbiota, and targeting specific cell populations, such as macrophages, with puerarin holds potential therapeutic value.

Abstract P343: Cerebral injury and cognitive performances are correlated with immunoinflammatory markers in hypertensive patients

Immunity and inflammation play a pivotal role in hypertension onset and cardiovascular organ damage. This role has been thoroughly characterized for classical hypertension targets as the kidneys, the heart and the vasculature. Many studies have characterized this in the experimental context, however immunoinflammatory challenges links with brain injury in the clinical context of hypertension are still lacking. In this study we will characterize how immunoinflammatory mediators can be predictors of cerebral injury characterized by advanced neuroimaging in hypertensive patients. Hypertensive patients underwent cerebral MRI and by advanced neuroimaging we characterized the microstructural injury by DTI fiber tracking. We administered the Montreal Cognitive Assessment to evaluate cognitive function, measured circulating C-reactive protein (hs-CRP) and counted circulating white blood cells (WBC). We found a large cluster of white matter tracts whose integrity parameters were associated with immunoinflammatory biomarkers (Figure A). In particular, a cluster of tracts of the limbic system and encompassing the corpus callosum show a correlation between their loss of integrity and hs-CRP levels. Another cluster of associative tracts spanning the temporal lobe show association between their loss of integrity and the WBC. Finally, the Frontal Aslant and the Uncinate Fasciculus show association with both immunoinflammatory markers (Figure B). In this study we performed advanced neuroimaging analyses to characterize the interplay between immunoinflammatory risk and cerebral alterations in hypertensives. Furthermore, our previous work identified that white matter damage in the Forceps Minor and Superior Longitudinal Fasciculus was a typical trait of hypertensive patients and associated to loss of cognitive functions. Our data further show the inflammatory risk association with worse cognitive functions, suggesting a clinical relevance in terms of damage evidenced by advanced MRI in hypertensive patients.

Development of a sapphire microstrip detector for gamma beam monitoring

Artificial sapphire gained interest as radiation detector in the recent years, as an alternative to diamond. The research and development campaign to design, build and characterize a radiation-hard detector for gamma beam monitoring takes place in the scientific context of the Laser Und XFEL Experiment (LUXE) at DESY. The experiment requirements of beam profile reconstruction with micro-metric precision and radiation hardness led to a proposed sapphire microstrip detector design. This work introduces the scientific context for a sapphire microstrip gamma beam profiler (GBP) and describes the experimental campaign for sapphire characterization from early prototypes to the final development of a 192-strip detector.

U.S. Males and Pornography: Replication and Experimental Extension

ABSTRACT Communication scientists have published pornography research in the communication discipline’s central journals for decades. Health communication scholars have become particularly interested in pornography in recent years, given increasing evidence of its likely impact on critical sexual health outcomes. An important resource for scholarship on pornography use is the General Social Survey (GSS). The first major article on pornography use and potential effects using the GSS was published only a decade ago and in a sexological journal, however. The present study provides a replication of this original article within the context of a GSS methodological experiment designed to provide pornography scholars with the first opportunity in 50 years to test a potential new pornography use measure. Results are interpreted in terms of their implications for the GSS, the pornography literature in general, and multiple theories of media use, processes, and effects.

Mental representation of words and concepts in late multilingualism

Abstract Speakers of multiple languages must store the respective lexical items efficiently to enable correct access. Importantly, all items must be linked to semantic information and world knowledge. One prominent model of the mental lexicon of late bilinguals is the Revised Hierarchical Model (Kroll &amp; Stewart, 1994), which postulates bidirectional but asymmetrical connections between separate stores for L1 (native language) and L2 (second/foreign language), and a shared conceptual store. Using German native speakers with advanced English proficiency, Experiment 1 largely confirmed model predictions regarding different preferred mental routes and processing times depending on translation direction. Moreover, the original design was extended by including abstract stimuli and picture naming in L2. A series of additional measures, such as proficiency and age of acquisition, served to specify the language experience of the participants and made it possible to compare the results with a group of non-native speakers of German (Experiment 2). Interestingly, the results suggest that the model also applies to two or more non-native languages, potentially influenced by the experimental and environmental language context.

Improving the Accuracy of Bulk Fitness Assays by Correcting Barcode Processing Biases.

Measuring the fitnesses of genetic variants is a fundamental objective in evolutionary biology. A standard approach for measuring microbial fitnesses in bulk involves labeling a library of genetic variants with unique sequence barcodes, competing the labeled strains in batch culture, and using deep sequencing to track changes in the barcode abundances over time. However, idiosyncratic properties of barcodes can induce nonuniform amplification or uneven sequencing coverage that causes some barcodes to be over- or under-represented in samples. This systematic bias can result in erroneous read count trajectories and misestimates of fitness. Here, we develop a computational method, named REBAR (Removing the Effects of Bias through Analysis of Residuals), for inferring the effects of barcode processing bias by leveraging the structure of systematic deviations in the data. We illustrate this approach by applying it to two independent data sets, and demonstrate that this method estimates and corrects for bias more accurately than standard proxies, such as GC-based corrections. REBAR mitigates bias and improves fitness estimates in high-throughput assays without introducing additional complexity to the experimental protocols, with potential applications in a range of experimental evolution and mutation screening contexts.