Medicinal plants are beneficial for curing several ailments among traditional healers, indigenous people, local practitioners and forest dwellers. If harnessed, traditional knowledge of the ethnomedicinal plants can play a pivotal role in drug discovery and development. In many cases a single medicinal plant can be of multiple uses also, different plant parts of the same plant can be utilized for more than one disease condition. The Ethno-botanical, Phytochemical and physico-chemical characterization of five selected medicinal plants viz., Nyctanthes arbor – tristis, Andrographis paniculata, Cissus quadragularis, Plumbago zeylania and Costus specious is an essence and has been carried out to assess bioactive potential and to establish traditional belief in the light of scientific interpretation. Fresh leaves were collected from Indravati National Park, Bijapur, Chhattisgarh, India, dried and powdered for phytochemical and Proximate, ultimate, and compositional analysis. Study revealed that the highest concentration of Ash Content (18.513%), Moisture Content (8.56%), Carbon content (48.77%), Hydrogen Content (24.490%), Nitrogen Content (23.860%) was observed to occur in Plumbago zeylanica leaf than other experimental plants. In Cissus quadrangularis, the percentage composition of fat content (0.230%), extractive content (1.05%), Lignin Content (5.53%) was higher than others. Fat content (0.230%), Moisture Content (8.56%), Vit. C content (64.63%) and Oxygen contents (36.655 %) were observed to be higher in Nyctanthes arbor-tristis leaf than others. The concentrations of Crude Fibre (14.49 %), Moisture Content (8.56%), Protein content (12.16%), Carbon content (75.66 %), Cellulose content (47.63 %) were observed to be highest in Costus speciosus species than others. Carbon content (48.77%) and Hydrogen Content (24.490%) were higher in Andrographis paniculata than in others.
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