Introduction. The problem of infertility is not only medical but also social one that comes to the fore in times of economic crisis, reduced fertility and a high level of total mortality. Such phenomenon of infertility is more common than you think. The world percentage of infertile couples is 8-29%, in Ukraine - 15-18%, or each 4-5 pair is unable to realize their plans to have children naturally. The composition of the microbiota of vulvovaginal content in women with infertility of types I and II depends on many exogenous and endogenous factors, mainly etiological agents can be as pathogenic, and so opportunistic aerobic (S.aureus, S.epidermidis, N.gonorrhoae, E. coli, C.albicans) and anaerobic microorganisms. The article compared the species composition of the microbial vulvovaginal content of women with infertility types I and II, which may be involved in the formation of certain dysbiotic disorders.Materials and methods. There were examined 89 women of reproductive age with type I of infertility. The average age was 27.00 ± 2,37 years. Also there was conducted bacteriological and mycological examination of vulvovaginal content of 67 women with infertility of type II aged from 18 to 34 years (average age - 26,00 ± 1,12 g.).In all women for bacteriological and mycological examinatuin it was taken vaginal content in sterile test tubes in sterile conditions. To the material it was added tenfold volume of sterile isotonic sodium chloride solution to get 1:10 (10-¹) dilution. On a base of the resulting mixture there was prepared titration series (10-2 to 10-7), from which it was seeded 0.01 ml to sectors of the optimal culture media fer each taxon evenly distributing on the surface of solid media or semisolid media.Facultative anaerobic and aerobic bacteria were grown in a thermostate (temperature - 37 ºC, 18-24 hours, yeast-like fungi of the genus Candida - 30 ºC). Obligate anaerobic bacteria were grown in a stationary anaerostate (CO2 incubator T-125 Medicin - Sweden) for 5-7 days, sometimes up to 14 days. Identification of selected taxons was conducted by morphological, tinctorial, cultural, biochemical properties, according to necessity there were determined signs of pathogenicity and antigenic structure.Results. We found that in women with infertility of type I S.aureus was isolated in 56 (62,9 %) patients, S.epidermidis - in 11 (12,3 %) patients, N.gonorrhoeae - in 8 (8,9 %) patients, E.coli - in 10 (11,4 %) patients, C.albicans - 66 (74,1 %) patients, Lactobacillus - in 28 (31,4 %) patients, Bifidobacterium - in 13 (14,6 %) patients, Peptostreptococcus - in 23 (25,8 %) patients, Propionibacterium - in 3 (3,3 %) patients, T.vaginalis - in 52 (58,4 %) patients.In vulvovaginal content of women with infertility of type II there dominated conditionally pathogenic yeast-like fungi of the genus Candida, Staphylococcus aureus, vaginal Trichomonas, Peptostreptococci and Enterobacteria.Conclusions. One of the causative agents of development of infertility is a colonization of vulvovaginal content by opportunistic pathogens of genus Candida, bacteria of the genus Staphylococcus, vaginal Trichomonas, Peptostreptococci, Enterobacteria, N. gonococcus, Bacteroides and other microorganisms as well as their associations. In the vulvaginal content of women with type I infertility, autochthonous obligate anaerobic microorganisms of the genera Lactobacillus and Bifidobacterium are isolated in 46.0% of cases, and in women with type II infertility only in 28.2% of women (P <0.05), which causes a decrease in contamination and colonization of the biotope by 2.27 times by enterobacteria (E. coli, K. pneumoniae) in women with primary infertility. In these women the colonization of vulvovaginal contents by yeast-like fungi of the genus Candida increases (by 10.43%), protozoa (T. vaginalis) by 26.41% and microorganisms of the genus Peptostreptococcus - by 91.96%. The difference in the detection of other opportunistic bacteria (S. aureus, N. gonorrhoeae, etc.) is insignificant (P> 0.05).