Content Of Total Polyphenolic Compounds Research Articles

Effect of Grinding and Successive Sieving on the Distribution of Active Biological Compounds in the Obtained Fractions of Blackthorn Berries

The current study evaluated the effect of powder fractionation based on particle size on the chemical composition of macronutrients such as proteins and sugars, on the phytochemical properties (total content of polyphenolic compounds, vitamin C, and antioxidant activity), on preservation capacity (water activity), powder functional properties (water absorption capacity and water solubility index), and physicochemical properties (particle size distribution and moisture content) of blackthorn berry (Prunus spinosa) powders. The fruits were separated from the plant material and seeds, dried, and then ground using an universal mill for dry materials. Eight fractions were obtained after sieving on sieves with different mesh sizes, such as 1 mm, 0.8 mm, 0.630 mm, 0.450 mm, 0.315 mm, 0.200 mm, and 0.125 mm. The grinding/sieving procedure was effective in separating Prunus spinosa powder into sufficiently different size classes. The maximal moisture content and water activity were 5.61% and 0.250, respectively, showed good preservation from a microbiological point of view, and ensured the prevention of oxidation of biologically active compounds of blackthorn berry powders. For samples with reduced particle sizes, the powder functional properties were greatly improved. The total phenolic content, carbohydrates, and antioxidant activity showed significantly different values for some particle size classes compared to the un-sieved sample. A considerable content of vitamin C was presented in the fraction with large particle sizes, precisely because they did not undergo intense degradation processes. Therefore, the technique of grinding and successive sieving proved effective in enhancing the physicochemical and functional characteristics of powdered blackthorn berries, particularly for smaller particles.

Accumulation of Total Content of Polyphenol Compounds and Antioxidant Activity of Echinacea Moench Species

Accumulation of Total Content of Polyphenol Compounds and Antioxidant Activity of Echinacea Moench Species

The use of blackcurrant pomace and erythritol to optimise the functional properties of shortbread cookies

As a result of the production of blackcurrant juice, pomace is produced, which is a cheap, easy to further process raw material with high health benefits. The aim of the research was to develop a recipe for shortbread cookies based on blackcurrant pomace (0, 10, 30, 50%) and erythritol, and to assess their nutritional value (content of proteins, fats, sugars, dietary fibre, selected minerals and energy value), pro-health properties (antioxidant and anti-diabetic capacity) and sensory evaluation. The energy value of products with 50% of pomace sweetened with erythritol was nearly 30% lower compared to traditional cookies, while the content of dietary fibre was 10 times higher in products with the highest percentage of pomace. The antioxidant capacity and the total content of polyphenolic compounds increased with the increase in pomace content. The ability to inhibit α-amylase by shortbread cookies without pomace was about 400 times lower than those with 50% pomace. The results of the sensory evaluation showed that erythritol-sweetened cookies have more desirable characteristics compared to sucrose-sweetened cookies. Finally, it was proved that the proposed products are an excellent proposal for people struggling with food-dependent diseases, as well as being an opportunity to manage waste from the fruit industry.

Determination of the total content of metals, polyphenols, flavonoids and the antioxidant capacity of methanolic and chloroformic extracts from Cichorium intybus

he study aimed to assess the metal content, total polyphenols, and flavonoids in the plant Cichorium intybus L gathered from Bihor, Romania, and to emphasize the antioxidant activity of its bioactive constituents. The materials and methods involved the extraction of two types of extracts from the Cichorium intybus L plant using the Soxhlet extraction method. The extracts were prepared using two different solvents, namely 50% methanol and chloroform. The purpose of this extraction was to assess and compare the total phenolic and flavonoid content, as well as to determine the antioxidant capacity of the extracts using the DPPH and FRAP methods. The methanolic chicory extract had a higher total content of polyphenolic compounds (57.98 ± 0.60 mg GAE/g dry floss) and flavonoids (5.94 ± 0.08 mg QE/g dry floss) compared to the chloroform extract (55.28 ± 1.11 mg GAE/g dry floss) and (5.54 ± 0.13 mg QE/g dry floss). This indicates that methanol is a more effective solvent for extraction than chloroform. The results of the study indicate that the chloroform chicory extract exhibited a higher percentage of inhibition (74.10 ± 0.22%) compared to the methanolic extract (67.47 ± 0.65%) when tested for antioxidant capacity using the DPPH method. Conversely, the methanol extract from chicory demonstrated a higher antioxidant capacity (856.80 µmol TE/g dry chicory) than the chloroform extract (771 µmol TE/g dry chicory) according to the FRAP method. The Cichorium intybus L plant has the potential to yield phytopharmaceutical compounds that can help maintain or enhance human health.

Influence of Growth Regulators and Different Spectra of Monochromatic Radiation on the Growth and Biosynthetic Characteristics of Callus Culture of Ipomoea batatas (L.) Lam.

The work studied the influence of plant growth regulators as well as the effect of monochromatic radiation of different spectral composition on the growth and accumulation of secondary metabolites in the callus culture of sweet potato (Ipomoea batatas L.). It was found that auxin analogues in low concentrations (0.5 mg/L) significantly stimulated the growth of cell biomass, while the effect of 4-chlorophenoxyacetic acid (4-CPA) was more pronounced (stimulation 16 times) than that of 2,4-dichlorophenoxyacetic acid (2,4-D) (13.5 times stimulation). Both the increase in the concentration of 2,4-D and 4-CPA in the medium and the addition of cytokinin, 6-benzylaminopurine (BAP), led to a significant inhibition of culture growth (up to three times). In contrast to 2,4-D, the addition of 4-CPA led to an eightfold increase in the total content of polyphenolic compounds in cultured cells. Activating effect of 4-CPA on the biosynthetic characteristics of I. batatas cell culture persisted even with the addition of BAP, while an increase in the concentration of 4‑CPA led to the leveling of the activating effect. Monochromatic radiation—white, red (660 and 630 nm), yellow, green, and blue (440 and 460 nm) light—inhibited growth (up to 1.5 times) and the total accumulation of secondary metabolites in I. batatas cells (up to 1.8 times). At the same time, the white, bright blue, and red spectra differentially activated the formation of individual compounds 3,4-dicaffeoylquinic acid and 3-feruloyl-5-caffeoylquinic acid. Thus, the authors have established that both auxin analogues and spectral radiation exhibit different effects on the growth and biosynthetic characteristics of the I. batatas calli.

EXTRACTION OPTIMIZATION AND PHENOLIC COMPOUNDS ANALYSIS IN FICUS CARICA L. FRUITS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

One of the important tasks of modern pharmacology and pharmacy is the search and development of safe and effective medicines. On the pharmaceutical market of Ukraine, there is a trend of development of organic chemistry and the emergence of new synthetic medicines. However, the search and in-depth study of plant raw materials remains relevant, as medicinal products based on it show low toxicity and have high indicators of biological activity. According to literature sources, ripe fruits of Ficus carica L. of the Moraceae family are a promising object for further research. It is known that the fruits of Ficus carica L. contain a whole complex of biologically active substances that provide them with antioxidant, antiinflammatory, choleretic and antispasmodic effects. The aim of the work was to study the qualitative composition and quantitative content of biologically active compounds of phenolic nature in ripe fruits of Ficus carica L. by the method of high-performance liquid chromatography. The fruits of Ficus carica L. were extracted under different conditions and methods of extraction. Various concentrations of the water-ethanol mixture (30%, 50% and 70%) were chosen as the solvent, with a ratio of solvent to plant material of 1:8 and grinding of the plant material to a size of 5 mm. A series of extractions was carried out in a Soxhlet apparatus and by the maceration method until the target product was completely extracted. The main criterion for evaluating the optimal conditions and extraction method was the quantitative content of phenolic compounds, which was determined spectrophotometrically according to the Folin-Chocalteu method in terms of gallic acid. It was established that the optimal conditions that ensure the maximum extraction of polyphenolic compounds from plant raw materials are the use of 70% alcohol as an extractant when using both extraction methods. It has also been proven that higher rates of target product yield are observed when extracted in a Soxhlet apparatus. Using the method of high-performance liquid chromatography at a wavelength of 225 nm, it was established that the extract of Ficus carica L. contains such groups of phenolic compounds as: phenolic acids (101.4 μg/ml), catechins (98.08 μg/ml), catechin-like substances (599.29 μg/ml), flavanols (1.07 μg/ml), flavonol-like (43.10 μg/ml), flavonoids (99.15 μg/ml). The total content of polyphenolic compounds in the studied sample of the extract from the fruits of Ficus carica L. is 1420.28 μg/ml, where the share of unidentified compounds is 478.19 μg/ml.

Cotinus coggygria Scop. Attenuates Acetic Acid-Induced Colitis in Rats by Regulation of Inflammatory Mediators.

In traditional medicine, many medicinal plants are used in the treatment of various diseases caused by inflammation. The objective of the present study is to elucidate for the first time the effects of Cotinus coggygria (CC) ethanol extract (CCE) on colonic structure and inflammation of acetic acid-induced ulcerative colitis in rats. Colonic damage was assessed using disease activity index score, enzyme-linked immunosorbent assay, and hematoxylin-eosin staining. Also, in vitro antioxidant activity of CCE was investigated by ABTS methods. Total phytochemical content of CCE was measured spectroscopically. Acetic acid caused colonic damage according to disease activity index and macroscopic scoring. CCE significantly reversed these damages. While the levels of proinflammatory cytokines TNF-alpha, IL-1beta, IL-6, and TGF-1beta increased in tissue with UC, IL-10 level decreased. CCE increased inflammatory cytokine levels to values close to the sham group. At the same time, while markers indicating disease severity such as VEGF, COX-2, PGE2, and 8-OHdG indicated the disease in the colitis group, these values returned to normal with CCE. Histological research results support biochemical analysis. CCE exhibited significant antioxidant against ABTS radical. Also, CCE was found to have a high content of total polyphenolic compounds. These findings provide evidence that CCE might be benefit as a promising novel therapy in the treatment of UC in humans due to high polyphenol content and justify the use of CC in folkloric medicine for treatment of inflamed diseases.

The Influence of the Use of Different Polysaccharide Coatings on the Stability of Phenolic Compounds and Antioxidant Capacity of Chokeberry Hydrogel Microcapsules Obtained by Indirect Extrusion.

The aim of the study was to evaluate the effect of the use of different polysaccharides and their mixtures on the structure of chokeberry hydrogel microcapsules and the stability of polyphenolic compounds and antioxidant capacity during one month of refrigerated storage. As a coating material, alginate and its mixtures with pectin, carrageenan, and chitosan were used, while chokeberry juice and preparation of polyphenolic compounds were used as a core. In non-stored capsules, the addition of carrageenan, pectin, and chitosan to alginate increased the content of total polyphenolic compounds and antioxidant capacity. When compared to non-encapsulated juice, the capsules had a greater decrease in antioxidant capacity during storage. The coating variant composed of alginate and carrageenan was found to be the most beneficial for the preservation of the capsules' phenolic compounds. The findings revealed that proper polysaccharide coating selection is critical for the proper course of the microencapsulation process, the polyphenolic content of chokeberry capsules, and their antioxidant properties.

Proximate Composition and Antioxidant Activity of Selected Morphological Parts of Herbs

The aim of the study was to provide an analytical evaluation of the proximate composition, the total content of polyphenolic compounds and the antioxidant activity, of 27 selected plant materials collected in Poland (West Pomeranian). The basic chemical composition was determined in the ground samples according to the Association of Official Analytical Chemists methods. Antioxidant activity was tested using free radical methods ABTS•+, DPPH•+ and the FRAP method. The lowest concentration of dry matter (DM) was measured in black chokeberry (88.82 g/100 g) and the highest was found in milk thistle (94.65 g/100 g) as well as black cumin (95.09 g/100 g). The content of total polyphenols, assessed using the Folin–Ciocalteu method, ranged from 291.832–7565.426 mg of chlorogenic acid equivalent (CGA)/100 g of DM. Antioxidant activity measured sequentially against the radical ABTS•+, DPPH•+ and using the FRAP method was 26.334–1912.016 µM Trolox/g DM, 9.475–1061.068 µM Trolox/g DM and 26.252–1769.766 µM Trolox/g DM, respectively. The methanolic extract from milk thistle fruit in most assays was characterized by the lowest antioxidant activity and the lowest total content of polyphenolic compounds. Methanol extracts prepared from garlic, stinging nettle and cleavers showed the highest content of total polyphenols and antioxidant activity among the tested plant materials. The parts of plants with the highest antioxidant potential can be a source of new bioactive compounds, but further research is required to describe the profile of compounds harmful to human health.

SCREENING OF CONTAINS OF ANTIOXIDANTS IN TRADITIONAL WINES OF THE TRANSCARPATHIAN REGION

Antioxidants play an important role in human health by performing various functions, including anticancer. Among antioxidants, the most common are polyphenols, including anthocyanins, the main sources, of which are berries and fruits. Red grape wines are a potentially important source of antioxidants, and red wine polyphenols have anticancer and antioxidant effects and have a positive effect on blood red blood cells. This is obviously related to the so-called "French paradox" of wine, because the regular consumption of dry red wine by the French dramatically reduces the incidence of cardiovascular disease and cancer.
 Transcarpathia has long traditions of grape wines production, known aboard of the country. This was facilitated by special climatic conditions, soil factors of the grape provinces of Transcarpathia and the skill of the winemakers. In this work we report, a high total content of polyphenolic compounds and anthocyanins in red wines derived from European grape varieties based on the screening of antioxidant content in traditional wines of Transcarpathia. Wines from grapes of the hybrid variety "Isabella" and European varieties "Merlot" and "Cabernet Sauvignon" were studied in three main wine-growing regions of the region. Among the studied red wines (harvested in 2020), the highest total content of polyphenolic compounds is observed in wine from Merlot grapes (2.75 g´L-1), which was obtained in Berehove district. In the same wine, there is the highest total content of anthocyanins (0.715 g´L-1, is 26% of the gross content of polyphenols). Such wine can have a high anti-cancer effect, and its consumption in moderate amounts will have a preventive effect on human health. At the same time, it is shown that the antioxidant properties of red grape wines persist for a short time after uncorking the bottles.

The Yeast Fermentation Effect on Content of Bioactive, Nutritional and Anti-Nutritional Factors in Rapeseed Meal.

The aim of this study was to evaluate the changes in the content of bioactive, nutritional and anti-nutritional factors in rapeseed meal that was fermented with Saccharomyces cerevisiae or Saccharomyces boulardii yeasts at two different periods of time, for improvement of nutritional characteristics in piglets’ feeding. The fermentation has reduced the content of two anti-nutritional factors, intact glucosinolates and 3-butyl isothiocyanate, by 51.60–66.04% and 55.21–63.39%, respectively, by fermentation with either Saccharomyces cerevisiae or Saccharomyces boulardii for 24 h. The fermentation by these yeasts also lowered the content of total polyphenolic compounds by 21.58–23.55% and antioxidant activity (DPPH) by 17.03–21.07%. Furthermore, the content of carbohydrates and organic acids has dramatically decreased between 89.20 and 98.35% and between 31.48 and 77.18%, respectively. However, the content of some individual phenolic acids (gallic, p-coumaric, sinapic) and crude protein content (10–13%) has been increased. Thus, the results showed that fermentation with Saccharomyces cerevisiae or Saccharomyces boulardii has reduced the content of antinutritive factors and increased the protein content of the rapeseed meal, without major adverse effects on its overall nutritive value.

The effect of light conditions on the content of selected active ingredients in anatomical parts of the oyster mushroom (Pleurotus ostreatus L.).

This study aimed to evaluate the effect of cultivation conditions in the context of light on the retention of selected vitamins, minerals and polyphenols in the stem and cap of the oyster mushroom (Pleurotus ostreatus L.). Additionally, the effect of the retention of bioactive components on the antioxidant activity of mushroom extracts was evaluated, taking into account the morphological part. Oyster mushrooms grown in the light of 200 lux had higher riboflavin content compared to mushrooms exposed to the light of lower intensity. The thiamine content of the mushrooms dropped with decreasing light intensity during cultivation. The content of biologically active compounds was found to be equal in the stem and the cap. In the case of riboflavin, it was shown that its contents in cap fractions, irrespective of the cultivation method, was statistically significantly higher than in stems. The mineral composition of caps and stems differed from each other. No differences in Zn and Cu content between the morphological parts of the mushroom studied were found. However, it was shown that the stems, regardless of the type of light, contained less iron, magnesium and sodium. Thus, it was observed that limited light exposure caused an increase in the content of total polyphenolic compounds, which did not correlate with antioxidant activity. There was no effect of the light on the antioxidant activity of mushrooms. It was also shown that stem extracts had higher antioxidant activity compared to the extracts obtained from the caps. This findings point to the possibility and potentail of use both fraction of mushrooms in the new food products development.

WPŁYW CZASU PRZECHOWYWANIA NA WŁAŚCIWOŚCI ANTYOKSYDACYJNE I PROFIL POLIFENOLOWY MROŻONEGO I LIOFILIZOWANEGO CZERWIU TRUTOWEGO UTRWALONEGO W MIODZIE

Background. The purpose of the study was to evaluate the effect of drone brood fixation in rape honey and to analyze changes in its antioxidant activity during storage. The fixation was carried out for frozen and lyophilized drone brood, which were mixed with honey in various proportions (1, 2, and 4 % w/w). The antioxidant activity (DPPH and FRAP methods) and the total content of polyphenolic compounds (TPC) after 3, 6, and 9 months of storage were analyzed. After 9 months of storage, the polyphenolic profile (HPTLC method) and physicochemical parameters of samples were assessed. Results and conclusion. The addition of drone brood to honey increased the antioxidant activity of the final product considerably (by 33 to 110 %), while only slightly affected the physicochemical parameters (conductivity and a diastase number) compared to control honey. Moreover, honey with the addition of drone brood still continued to meet the requirements for honey standard. The polyphenol profile obtained by HPTLC method for honey with the addition of drone brood was enriched mainly with ellagic and ferulic acids compared to control honey. It was found that fixing the drone brood in honey allows to maintain its antioxidant properties for 6 months, whereas a significant decrease in reducing power (FRAP) and polyphenolic content (TPC) during prolonged storage were observed (from 8 to 26 % ). Due to the fact that lower losses were observed for the addition of frozen than for lyophilized drone brood after 9 months of storage, preserving the frozen brood in honey (up to 5 % w/w) can be recommended as an effective and inexpensive method available in apiary conditions.

Edible Flowers’ Antioxidant Properties and Polyphenols Content Reflect Their Applicability for Household and Craft Tincture Production

The growing interest of consumers in regional and traditional products drew our attention to innovative products manufactured at home and using craft methods, which include, among others, alcohol tinctures of edible flowers. The aim of this paper is to present selected tinctures of edible flowers from home and craft production, their phenol content, antioxidant properties and colour. Novel alcoholic beverages obtained from edible flowers are characterized. The tinctures from wild rose flowers, elderberry, marigold and cornflower were studied. The content of phenolic compounds, the antioxidant properties and the colour of tinctures in the CIE L*a*b* system were analysed. The study showed that edible flower tinctures are characterized by an intense colour, which is not adversely affected by the maceration process. The determined parameters were influenced by the form of flowers (fresh or dried). The total content of polyphenolic compounds and antioxidant activity in the studied tinctures were lower than in the fresh flowers. Edible flower tinctures can be an interesting alternative for both consumers looking for product innovations and alcohol connoisseurs.

Physiological Response of Maize Plants (Zea mays L.) to the Use of the Potassium Quercetin Derivative.

Plant production technologies based solely on the improvement of plants themselves face obstacles resulting from the natural limitations of the biological potential of varieties. Therefore, new substances are sought that positively influence the growth and development of plants and increase resistance to various biotic and abiotic stresses, which also translates into an increase in obtained yields. The exogenous application of various phytoprotectants shows great promise in terms of cost effectiveness compared to traditional breeding methods or transgenic approaches in relation to increasing plant tolerance to abiotic stresses. Quercetin is a strong antioxidant among phenolic compounds, and it plays a physiological and biochemical role in plants. As such, the aim of this research was to assess the effect of an aqueous solution of a quercetin derivative with potassium, applied in various concentrations (0.5%, 1.0%, 3.0% and 5.0%), on the efficiency of the photosynthetic apparatus and biochemical properties of maize. Among the tested variants, compared to the control, the most stimulating effect on the course of physiological processes (PN, gs, ci, CCI, Fv/Fm, Fv/F0, PI) in maize leaves was found in 3.0 and 5.0% aqueous solutions of the quercetin derivative. The highest total antioxidant capacity and total content of polyphenolic compounds were found for plants sprayed with 5.0% quercetin derivative solution; therefore, in this study, the optimal concentration could not be clearly selected.

Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves.

To elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatment of plant material (pH, enzyme concentration, time, and temperature) by response surface methodology (RSM). Medicago sativa L. was selected as a plant material due to its richness in phenolics and flavonoids. HPLC-MS/MS analysis allowed evaluating the content of individual bioactive compounds in obtained extracts. The total content of polyphenolic compounds in the extract obtained after two-step optimization was much higher (546 ± 21 µg/g) than in the extract obtained from non-hydrolyzed material (275 ± 23 µg/g) and in the extract obtained by maceration (162 ± 20 µg/g). Furthermore, it was evidenced that extract with the highest content of polyphenolic compounds can support the cellular antioxidant system both as a free radical scavenger and by stimulating the antioxidant enzyme system.

The study of polyphenolic compounds of Pimpinella anisum herb and determination of their antioxidant activity

Aim. To study the qualitative composition and quantitative content of the polyphenolic compounds in Pimpinella anisum herb and determine their antioxidant activity.Results and discussion. Among the polyphenolic compounds contained in anise herb, chlorogenic acid(4.409 mg/g) predominates. Signifcant amounts of catechins (3.104 mg/g), apigenin derivatives (3.077 mg/g) and luteolin (1.864 mg/g) also accumulate in the herb. Minor amounts of myricetin (0.105 mg/g) and naringenin (0.019 mg/g) derivatives, rutin (0.189 mg/g), quercetin (0.028 mg/g), apigenin (0.009 mg/g) and hesperetin (0.002 mg/g) are present. According to the research results, the antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid was found to be 67.76 ± 0.05 mmol/g. Rutin exhibited the antioxidant activity at the level of 3979.59 ± 0.08 mmol/g.Experimental part. Pimpinella anisum herb collected during the flowering stage in the summer of 2019 inthe Kharkiv region (Ukraine) was used for analysis. The analysis of 70 % ethanolic extract from anise herb was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversedphase, C18 modifed silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Identifcation of substances in the extract was carried out by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. The antioxidant activity was determined by the potentiometric method (pH meter – Hanna 2550, with redox electrode EZDO PO50) with reference to ascorbic acid.Conclusions. The qualitative composition and quantitative content of polyphenolic compounds in the ethanolic extract of anise herb have been determined by high performance liquid chromatography. The total content of polyphenolic compounds is 17.576 mg/g. The antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid has been found to be 67.76 ± 0.05 mmol/g.Key words: polyphenolic compounds; anise; herb; high performance liquid chromatography; antioxidant activity

The Study of Antioxidant Components in Grape Seeds

The paper deals with the study of antioxidant properties of extracts from vine seeds (Vitis vinifera L.) using spectrometric and chromatographic techniques. Ten vine varieties (Cerason, Laurot, Kofranka, Gewürztraminer, Hibernal, Blaufrankisch, Zweigeltrebe, Erilon, Palava, and Welschriesling) obtained from the years 2015, 2016, and 2017 were selected for the study. The antioxidant activity was determined spectrophotometrically using four fundamentally different methods; the content of total polyphenolic compounds was determined using the Folin–Ciocalteu method. In 2015, the content of 14 antioxidants (gallic acid, caffeic acid, coumaric acid, coutaric acid, ferulic acid, fertaric acid, trans-piceid, trans-piceatannol, rutin, quercetin-3-β-d-glucoside, quercitrin, myricetin, catechin, and epicatechin) were studied. The results of the study show the high content of antioxidant components in grape seeds and the differences in content in individual varieties and in individual years.

Location and tissue effects on phytochemical composition and in vitro antioxidant activity of Moringa oleifera

Moringa oleifera is a rich source of polyphenols, but its content and profile may vary according to environmental conditions, harvest season, and plant tissue. This study was carried out in order to determine the phenolic compounds by HPLC/MS, as well as, the in vitro antioxidant activity of extracts from leaflets and petioles of Moringa oleifera planted in three locations. The content of total polyphenolic compounds was estimated. In addition, hydrolyzable polyphenols were determined using the Folin-Ciocalteu technique, while, condensed polyphenols were estimated employing the butanol-HCl method. These chemical compounds were identified by high-performance liquid chromatography coupled to masses spectrometry (HPLC/MS). In vitro antioxidant activity of the extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylenebenzothiozoline-6-sulfonic acid (ABTS) and the ferric reducing antioxidant power (FRAP) methods. The polyphenolic compounds were found mainly in the leaflets. The HPLC/MS allowed the identification of 15 compounds of the hydroxycinnamic acid, anthocyanin, flavonols, methoxyflavone, tyrosol, dihydrochalcones, flavones, and methoxyflavone families. The ethanolic extracts showed high antioxidant activity for DPPH, ABTS, and FRAP, especially, those obtained from the leaflets produced at Ramos Arizpe, Coahuila. The amounts of condensed were higher than those of hydrolyzable polyphenols in the moringa grew at three environments. While the highest amount of condensed polyphenols was observed in moringa grew at Ramos Arizpe. Thus, the presence of these polyphenol compounds may explain the great antioxidant activity (98.45% inhibition) showed with the ABTS radical, in the moringa ethanolic extracts from Ramos Arizpe. These results indicate that moringa may have great potential as a natural antioxidant agent.

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia Viridis.

The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.