Total Iron Content Research Articles

Non-silicate iron in mineral-associated organic matter of agro-chernozems located on different positions on the slope

Data on total non-silicate iron (Fed) content in mineral-associated organic matter (MAOM) of agro-chernozems of different localization on the slope are presented. The distribution of total non-silicate iron in soil profile follows that of organic carbon (OC): the maximum values are confined to the surface horizons, and decrease down the profile. It has been revealed that the change in the content of non-silicate iron in the MAOM of the clay fraction in arable horizons of eroded agro-chernozems is a result of erosion processes, including their intensity, which also depends, among other things, on the slope steepness. A lower slope steepness (4°) contributes to an increase in the organic carbon loading on iron oxides, resulting in an increased in iron desorption capacity, while a higher slope steepness (6°) results in lower iron desorption capacity. The OC/Fed molar ratio is suggested as an additional indicator of the degree of soil erosion. In clay fractions, of both their position in the soil profile and their position on the slope, the predominant mechanism of organic matter stabilization was the formation of organic-iron complexes (OC/Fed > 10). In the Residue fraction of humus horizons, non-silicate iron seems to be presented mainly as iron carbonates (FeCO3), while the proportion of microaggregates (stable under the sonication), where OM is mainly humin, is relatively low against the background of the absolute dominance of primary minerals in this fraction (weight %). Most of the non-silicate iron of mineralassociated OM extracted by dithionite-citrate-hydrocarbonate is localized in the clay fraction – 2/3 or more.

Estimating parasite-condition relationships and potential health effects for fallow deer (Dama dama) and red deer (Cervus elaphus) in Denmark.

Parasites can exert a substantial influence on the ecology of wildlife populations by altering host condition. Our objectives were to estimate single and multiparasite-condition relationships for fallow deer (Dama dama) and red deer (Cervus elaphus) in Denmark and to assess potential health effects along the parasite burden gradient. Fallow deer hosted on average two endoparasite taxa per individual (min=0, max=5) while red deer carried on average five parasite taxa per individual (min=2, max=9). Body condition of both deer species was negatively related to presence of Trichuris ssp. eggs while body condition of red deer was positively related to antibodies of the protozoan Toxoplasma gondii. For the remaining parasite taxa (n=12), we either found weak or no apparent association between infection and deer body condition or low prevalence levels restricted formal testing. Importantly, we detected a strong negative relationship between body condition and the sum of endoparasite taxa carried by individual hosts, a pattern that was evident in both deer species. We did not detect systemic inflammatory reactions, yet serology revealed reduced total protein and iron concentrations with increased parasite load in both deer species, likely due to maldigestion of forage or malabsorption of nutrients. Despite moderate sample sizes, our study highlights the importance of considering multiparasitism when assessing body condition impacts in deer populations. Moreover, we show how serum chemistry assays are a valuable diagnostic tool to detect subtle and sub-clinical health impacts of parasitism, even at low-level infestation.

Synthesis and characterization of green rust–deposited MoS2 composites for adsorptive removal of EDTA-chelated Ni(II) in wastewater

Ethylenediamminetetraacetatonickel(II) (EDTA-Ni(II)) has emerged as a significant soil and groundwater contaminant due to the increasing agricultural and industrial activities, posing environmental challenges. This study focuses on addressing the reactivity of green rust (GR), which can be hindered by oxidation with oxygen, limiting its effectiveness in remediation processes. To overcome this limitation and enhance the adsorptive capacities, the combination of sulfate green rust (SO4-GR) with various Fe(II)/Fe(III) ratios with a high-surface-area adsorbent, MoS2, resulting in the formation of binary composites of green rust-deposited MoS2 (MSGs) were explored. The aim was to improve the removal efficiency of EDTA-Ni(II) from contaminated wastewater. To characterize the MSGs, a comprehensive analysis using XRD, SEM, TEM, FTIR, and X-ray absorption spectroscopy was performed. The surface areas of the MSGs were smaller than that of MoS2 but larger than that of the SO4-GRs, indicating a promising composite material. XANES spectra analysis revealed that both MSGs and SO4-GRs exhibited a mixture of ferrous and ferric ions, as evident from their spectral positioning between FeO and Fe2O3. The optimal pH for efficient removal of EDTA-Ni(II) was 3, which resulted in removal efficiencies of 45.6%, 47.3%, 46.0%, and 46.2% for MSG 1, MSG 2, MSG 3, and MSG 4 after 24 h, respectively. Reducing the initial concentration of EDTA-Ni(II) to 50 mg Ni(II)/L effectively doubled the removal efficiency. Notably, as EDTA-Ni(II) was removed, an increased leaching of iron was observed, leading to a total iron concentration exceeding 40 mg/L for the composites with higher Fe(II)/Fe(III) ratios. These findings underscore the potential of MSG as a promising material for degrading EDTA-Ni(II) in contaminated wastewater, offering a viable solution to mitigate the environmental impact of this emerging contaminant. This study contributes to the understanding of green rust reactivity and provides valuable insights for developing effective strategies to address the challenges associated with EDTA-Ni(II) contamination.

Distributions of total mercury and methylmercury and regulating factors in lake water and surface sediment in the cold-arid Wuliangsuhai Lake region.

This study aimed to understand the occurrence of mercury in the water environment of typical cold and arid lakes and the regulating environmental factors. Water and surface sediment samples were collected from July to August, 2022 in the Wuliangsuhai Lake region for the analysis of total mercury (THg) and total methylmercury (TMeHg). Lake water THg and TMeHg ranged between 19.20 ~ 668.10 and 0.10 ~ 11.40ng/L, respectively, exceeding China's environmental quality standards and contents of other lakes and reservoirs in China and other areas. Surface sediments showed lower mean THg and TMeHg of 261.85 and 0.18μg/kg, respectively, with the former significantly exceeding the background value of Inner Mongolia and unpolluted natural lakes but lower than those of lakes affected by human factors, such as aquaculture. Sediments showed relatively low methylation and TMeHg (0.01-0.21%) concentrations. Correlation analysis identified salinity, total dissolved solids, conductivity, and redox potential as important factors affecting mercury speciation in water, whereas those in surface sediments were organic matter, pH, and total iron content. This study conducted preliminary research on the different species of Hg in Wuliangsuhai Lake water environment, which can provide scientific evidence for the specific treatment of Hg pollution in agriculture, or industry and other related fields. Our results suggest that upstream and downstream regulatory agencies should strengthen the regulation of agricultural and industrial production, moderately reduce human activities, and reduce the use of mercury-rich substances such as pesticides.

Hydroxysafflor Yellow A Alleviates Acute Myocardial Ischemia/Reperfusion Injury in Mice by Inhibiting Ferroptosis via the Activation of the HIF-1α/SLC7A11/GPX4 Signaling Pathway.

Ferroptosis is closely associated with the pathophysiology of myocardial ischemia. Hydroxysafflor yellow A (HSYA), the main active ingredient in the Chinese herbal medicine safflower, exerts significant protective effects against myocardial ischemia/reperfusion injury (MI/RI). The aim of this study was to investigate the protective effects of HSYA against MI/RI and identify the putative underlying mechanisms. An in vivo model of acute MI/RI was established in C57 mice. Subsequently, the effects of HSYA on myocardial tissue injury were evaluated by histology. Lipid peroxidation and myocardial injury marker contents in myocardial tissue and serum and iron contents in myocardial tissue were determined using biochemical assays. Mitochondrial damage was assessed using transmission electron microscopy. H9C2 cardiomyocytes were induced in vitro by oxygen-glucose deprivation/reoxygenation, and ferroptosis inducer erastin was administered to detect ferroptosis-related indicators, oxidative-stress-related indicators, and expressions of ferroptosis-related proteins and HIF-1α. In MI/RI model mice, HSYA reduced myocardial histopathological damage, ameliorated mitochondrial damage in myocardial cells, and decreased total cellular iron and ferrous ion contents in myocardial tissue. HSYA increased the protein levels of SLC7A11, HIF-1α, and GPX4 and mitigated erastin- or HIF-1α siRNA-induced damage in H9C2 cells. In summary, HSYA alleviated MI/RI by activating the HIF-1α/SLC7A11/GPX4 signaling pathway, thereby inhibiting ferroptosis.

Magnetic properties of iron-filled hydrogel clusters: a model system for quantitative susceptibility mapping with MRI

Quantitative approaches in clinical Magnetic Resonance Imaging (MRI) benefit from the availability of adequate phantoms. Ideally, the phantom material should reflect the complexity of signals encountered in vivo. In the present study we validate and characterize clusters consisting of sodium-polyacrylate embedded in an alginate matrix that are unloaded or loaded with iron for Quantitative Susceptibility Mapping (QSM), yielding a non-uniform iron distribution and tissue-mimicking MRI properties. Vibrating sample magnetometry (VSM) was used to characterize the phantom material and verify the accuracy of previous MRI-based observations of the QSM-based molar susceptibility (χM). MRI at 14.1 T with high resolution acquisitions was used to determine the size of hydrogel clusters and to further investigate the suitability of the phantom material as a model system for QSM at high field. VSM demonstrated that the iron-solution used for manufacturing the phantoms consisted of ferric iron. The χM of clusters with a constant iron-to-polyacrylate-ratio (8.3 μg/mg) observed with VSM was 50.7 ± 8.0 ppb mM−1 but showed a tendency towards saturation at total iron concentrations >1 mM. On unwrapped and background corrected phase-images obtained with gradient-echo MRI and an isotropic voxel size of 37 μm at 14.1T, the iron-free clusters had a roundish shape and blurry border with an equivalent sphere diameter of 276 ± 230 µm and a QSM of 7 ± 7 ppb. Iron-loading led to strong phase wrapping, necessitating the use of short echo times, or short inter-echo delays below 10 ms at 14.1 T. The equivalent sphere diameter of the iron-loaded clusters was estimated to 400–500 µm as verified using different MRI modalities (spin-echo, inversion recovery, and gradient echo MRI). With a constant iron-to-polyacrylate ratio, the cluster density was 10 mm−3 mM−1 iron. In agreement with previous observations, χM of samples with a constant amount of polyacrylate was 50.6 ± 11.4 ppb mM−1 at 3 T while samples containing clusters with a constant iron-to-polyacrylate-ratio yielded χM = 56.1 ± 6.3 ppb mM−1 at 3T and 55.6 ± 0.7 ppb mM−1 at 14.1 T. In conclusion we found that the molar susceptibility of the proposed model system corresponds to that predicted for ferritin in vivo loaded with 3000 iron atoms. The reproducibility was within 12% across MR scanners, batches, and phantom types and compared well with results obtained with vibrating sample magnetometry.

Oxalic acid enhanced ferrous/persulfate process for the degradation of triclosan in soil: Efficiency, mechanism and a column study

Triclosan (TCS) is a compound in pharmaceutical and personal care products (PPCPs) that preferentially accumulates in soil and may threaten human health. Iron-based persulfate activation processes (Fe-PAPs) are a promising technique in soil remediation but are limited by their narrow pH application range and resultant iron precipitation. In this study, oxalic acid (OA) was specifically introduced to improve the efficiency of Fe2+-activated persulfate (PS) processes for TCS degradation in soil. The complexation of OA and Fe2+ improved the total iron concentration in the Fe2+/OA/PS system and prevented iron precipitation. Fe-minerals in soil were also dissolved by OA, which participated in PS activation. The most appropriate proportion of Fe2+/OA/PS is 1:1:1, and approximately 85% of the TCS was degraded within 240 min compared with 45% in the Fe2+/PS system. SO4•- and 1O2 played important roles on TCS oxidation, while O2•- and CO2•- mainly participated in the iron cycle. The weak stretch of Fe-O in OA containing treatments in ATR-FTIR measurements provided evidence that OA dissolved the Fe-minerals. The introduction of OA in the Fe2+/PS system in four different soils reduced the negative effect of soil organic matter on TCS degradation. Three types of intermediates including hydroxylation, cleavage of ether bonds and cyclization products, were identified by LC-Q-TOF-MS. Column experiments were used to study the feasibility of in situ chemical oxidation (ISCO) remediation of TCS in the Fe2+/OA/PS system. Our findings indicated the ability of OA to be used as a cheating agent in Fe-PAPs for TCS soil remediation.

A study of biochemical changes in river buffaloes (Bubalus bubalis) naturally infected with Anaplasma marginale

Anaplasma marginale as an intracellular rickettsia, endemic in tropical and subtropical regions of the world. This study was performed to determine A. marginale infection, molecularly, and to investigate its effects on some serum biochemical parameters in river buffaloes referred to Ahvaz abattoir, Southwest Iran. A total of 103 apparently healthy river buffaloes were randomly sampled. Whole blood samples were subjected to PCR analysis and blood smears were examined microscopically for the presence of Anaplasma inclusions. Serum biochemical parameters including total protein, albumin, glucose, urea, iron, total bilirubin, total cholesterol, Calcium, Phosphorus and Magnesium concentrations, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities and also serum electrolytes comprising Na and K were assessed. The results revealed that 16 and 32 samples were infected with A. marginale in microscopic and PCR assessment, respectively. Serum biochemical analysis showed no significant difference between A. marginale infected and non-infected buffalo groups. It can be concluded that infection with A. marginale does not lead to much pathogenicity in river buffaloes, so that in infected animals, no clinical signs or abnormalities in the performance indicators of various organs can be detected at the laboratory level. However, the role of this species as a reservoir of this microorganism in nature and in the epidemiology and transmission of anaplasmosis to other species remains significant. Buffaloes can be a substitute to raising cattle in regions with high occurrence of anaplasmosis.

Pecan Biomass and Dairy Manure Utilization: Compost Treatment and Soil In-Situ Comparisons of Selected Pecan Crop and Soil Variables

A compost program was developed on-farm, utilizing tree trimming biomass from a commercial pecan farm comprised of 14-year-old improved cultivar Western Schley pecan (Carya illinoinensis) tree stands. The direct soil application of shredded pecan tree biomass (P) and dairy manure (M) served as a standard on-farm practice. Three composts were produced using P and M with varying levels of other inputs and processing. The PM compost contained only P and M and its production included only weekly turning and watering. The other two composts included P, M, unfinished compost, and clay inputs, and either additional landscaping residues (A) (designated PM/A compost) or “green chop” (on-farm grown legumes, G) (designated PMG/A compost); production of PM/A and PMG/A composts included additional processing steps intended to improve compost quality per the recommendations of a compost consulting company. Soil samples were taken at three depths (0–15 cm, 15–30 cm, 30–61 cm) in November 2017 from the 1.3 ha study plot of trees. The standard practice and compost treatments were applied at approximately 18 t/ha in January 2018 and 2019 at a 15 cm depth. Soils were re-sampled at the end of the two-year study. Composts and soils were analyzed for: pH, sodium adsorption ratio (SAR), electrical conductivity, and total carbon, organic matter, magnesium, calcium, sodium, nitrate-N, total Kjeldahl nitrogen (TKN), available phosphorus, potassium, zinc, manganese, iron, and copper contents. Pecan tree leaf nutrient content, stem water potential, and leaf greenness were also measured one and two years after soil amendment application. While increases in several soil properties were observed with the treatments, only available phosphorus content was significantly different between pre and post at all depths. Electrical conductivity, TKN, Fe, Cu, SAR, and Na content showed significant differences in the upper soil layers. No differences in leaf properties were observed. This suggests that there are minimal differences in the outcomes for compost application compared to in-situ biomass application; additional compost inputs and processing did not provide additional short-term soil or plant benefits for pecan tree production. More work is needed to determine if there are long-term benefits to soil quality, plant health and performance, or carbon sequestration that impact the economic and environmental decision-making processes for composting and application of local organic wastes.

P22-002-23 Development and Comparison of a Database for the Assessment of Heme, Non-Heme and Total Iron Content of Foods in Dietary Recalls – Does It Make a Difference?

P22-002-23 Development and Comparison of a Database for the Assessment of Heme, Non-Heme and Total Iron Content of Foods in Dietary Recalls – Does It Make a Difference?

Evaluation of the potential of recovering various valuable elements from a vanadiferous titanomagnetite tailing based on chemical and process mineralogical characterization.

In order to evaluate the potential of recovering various valuable elements from vanadiferous titanomagnetite tailing (VTMT), the chemical and process mineralogical characterization of VTMT were investigated in this study by various analytical techniques such as XRF, XRD, optical microscopy, SEM, EDS, and AMICS. It was found that VTMT is a coarser powder in general; about 50% of the particle size is greater than 54.30μm. The total iron content of the VTMT was 22.40 wt.%, and its TiO2 grade is 14.45 wt.%, even higher than those found in natural ilmenite ores. The majority of iron and titanium were located in ilmenite and hematite; 62.84% of hematite and 90.27% of ilmenite were present in monomeric form. However, there is still a portion of ilmenite and hematite embedded in gangue such as anorthite, diopside, and serpentite. For the recovery of valuable fractions such as Fe and TiO2 from VTMT, a treatment process including ball milling-high-intensity magnetic separation-one roughing and three refining flotation was proposed. Finally, a concentrate with TiO2 grade of 47.31% and total Fe (TFe) grade of 35.44% was produced; TiO2 and TFe had recovery rates of 57.71% and 28.23%, respectively. The recovered product is adequate as a raw material for the production of rutile. This study provides a reference and a new research direction for the recycling and comprehensive utilization of VTMT.

Encapsulation of atomized erythrocytes in liposomes as source of heme iron for oral supplementation strategies

AbstractThis work aimed to develop and characterize liposomes of atomized bovine erythrocytes (ABE‐liposomes) for potential use as a heme iron supplement or to fortify food with heme iron. Soy lecithin (SL) and Tween 80 (T80) were chosen to prepare empty liposomes (8 types), and ABE‐liposomes (16 types) by a rotary‐evaporated film method. Variables of interest for our study were: ABE concentration, proportion of SL:T80 and ultrasonic bath time. ABE‐liposomes were characterized according to appearance, color, morphology, Fourier transform infrared spectroscopy (FTIR), size, zeta potential, total iron content, encapsulation efficiency (EE) and in vitro iron release under gastrointestinal conditions. ABE‐liposomes presented a brown color and spherical shape. Hydrogen bridges were the main interactions in ABE‐liposomes. Their size ranged from 379 to 964 nm, the zeta potential from −56 to −74 mV, the iron concentration range was 0.8–9.0 mg/100 mL, and EE ranged from 29% to 48%. Ultrasonic bath time was the most important variable on the reduction of ABE‐liposomes' size and EE. Under in vitro gastrointestinal conditions, iron release from ABE‐liposomes occurred mainly at the intestinal level. In conclusion, ABE‐liposomes were effectively developed, which could become a complement for supplementation or food fortification strategies with nonheme for the prevention of iron deficiency anemia.Practical ApplicationsIn this study atomized bovine erythrocytes (ABE)‐liposomes were developed, due to the: (1) lack of supplements or products to fortify foods based on heme iron, (2) higher bioavailability of heme iron than nonheme iron, especially when working with ABE, and (3) ABE is a low value‐added product that is generated in slaughter plants and in turn is an excellent source of underutilized heme iron. In this study it was possible to develop ABE‐liposomes, where ABE was encapsulated inside liposomes, obtaining a product that could be used as a complement in iron supplementation strategies or food fortification to prevent or treat iron deficiency or iron deficiency anemia, especially in developing countries that have low consumption of heme iron.

Applicability of Zeolite from the Daubabinsk and Chankanai Deposits as a Sorbent for Natural Waters

This article examined the characteristics of zeolites from the Chankanai and Daubabinsk deposits. In the field of water treatment, the purpose of investigating natural zeolite from the Chankanai and Daubabinsk deposits as a sorbent presents new opportunities and prospects, as this raw material is little studied. The effectiveness of the sorbents was assessed based on the results of the water specifications according to the following criteria: methods for determining the total hardness of water; methods for measuring the mass concentration of total iron in water; methods for the determination of chloride in water; methods for the determination of nitrogen-containing substances in water; methods for determining the dry residue content of water; methods for determining the sulphate content of water; on hygiene requirements and water quality control. Zeolite modifications were aimed at improving their adsorption properties, increasing the efficiency of pollutant removal and developing optimal methods of using zeolites in water filtration. Modified zeolite from the Chankanai and Daubabinsk deposits showed high efficiency as a filter. When it was used to treat natural water, which normally contains high hardness and alkalinity, the best results were achieved with zeolite from the Chankanai deposit. In terms of Fe+ and Cl content, the best results were achieved using zeolite from the Daubabinsk deposit.

Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis.

Mid-gestational sevoflurane exposure may induce notable long-term neurocognitive impairment in offspring. This study was designed to investigate the role and potential mechanism of ferroptosis in developmental neurotoxicity induced by sevoflurane in the second trimester. Pregnant rats on day 13 of gestation (G13) were treated with or without 3.0% sevoflurane, Ferrostatin-1 (Fer-1), PD146176, or Ku55933 on three consecutive days. Mitochondrial morphology, ferroptosis-relative proteins, malondialdehyde (MDA) levels, total iron content, and glutathione peroxidase 4 (GPX4) activities were measured. Hippocampal neuronal development in offspring was also examined. Subsequently, 15-lipoxygenase 2 (15LO2)-phosphatidylethanolamine binding protein 1 (PEBP1) interaction and expression of Ataxia telangiectasia mutated (ATM) and its downstream proteins were also detected. Furthermore, Morris water maze (MWM) and Nissl's staining were applied to estimate the long-term neurotoxic effects of sevoflurane. Ferroptosis mitochondria were observed after maternal sevoflurane exposures. Sevoflurane elevated MDA and iron levels while inhibiting GPX4 activity, and resultant long-term learning and memory dysfunction, which were alleviated by Fer-1, PD146176, and Ku55933. Sevoflurane could enhance 15LO2-PEBP1 interaction and activate ATM and its downstream P53/SAT1 pathway, which might be attributed to excessive p-ATM nuclear translocation. This study proposes that 15LO2-mediated ferroptosis might contribute to neurotoxicity induced by maternal sevoflurane anesthesia during the mid-trimester in the offspring and its mechanism may be ascribed to hyperactivation of ATM and enhancement of 15LO2-PEBP1 interaction, indicating a potential therapeutic target for ameliorating sevoflurane-induced neurotoxicity.

Variations in the archaeal community and associated methanogenesis in peat profiles of three typical peatland types in China

BackgroundPeatlands contain about 500 Pg of carbon worldwide and play a dual role as both a carbon sink and an important methane (CH4) source, thereby potentially influencing climate change. However, systematic studies on peat properties, microorganisms, methanogenesis, and their interrelations in peatlands remain limited, especially in China. Therefore, the present study aims to investigate the physicochemical properties, archaeal community, and predominant methanogenesis pathways in three typical peatlands in China, namely Hani (H), Taishanmiao (T), and Ruokeba (R) peatlands, and quantitively determine their CH4 production potentials.ResultsThese peatlands exhibited high water content (WC) and total carbon content (TC), as well as low pH values. In addition, R exhibited a lower dissolved organic carbon concentration (DOC), as well as higher total iron content (TFe) and pH values compared to those observed in T. There were also clear differences in the archaeal community between the three peatlands, especially in the deep peat layers. The average relative abundance of the total methanogens ranged from 10 to 12%, of which Methanosarcinales and Methanomicrobiales were the most abundant in peat samples (8%). In contrast, Methanobacteriales were mainly distributed in the upper peat layer (0–40 cm). Besides methanogens, Marine Benthic Group D/Deep-Sea Hydrothermal Vent Euryarchaeotic Group 1 (MBG–D/DHVEG–1), Nitrosotaleales, and several other orders of Bathyarchaeota also exhibited high relative abundances, especially in T. This finding might be due to the unique geological conditions, suggesting high archaeal diversity in peatlands. In addition, the highest and lowest CH4 production potentials were 2.38 and 0.22 μg g−1 d−1 in H and R, respectively. The distributions of the dominant methanogens were consistent with the respective methanogenesis pathways in the three peatlands. The pH, DOC, and WC were strongly correlated with CH4 production potentials. However, no relationship was found between CH4 production potential and methanogens, suggesting that CH4 production in peatlands may not be controlled by the relative abundance of methanogens.ConclusionsThe results of the present study provide further insights into CH4 production in peatlands in China, highlighting the importance of the archaeal community and peat physicochemical properties for studies on methanogenesis in distinct types of peatlands.

A Fast and Efficient Procedure of Iron Species Determination Based on HPLC with a Short Column and Detection in High Resolution ICP OES.

The optimization and application of a new hyphenated procedure for iron ionic speciation, i.e., high performance liquid chromatography (HPLC) with short cation-exchange column (50 mm × 4 mm) coupled to high resolution inductively coupled plasma optical emission spectrometry (ICP hrOES), is presented in this paper. Fe(III) and Fe(II) species were separated on the column with the mobile phase containing pyridine-2,6-dicarboxylic acid (PDCA). The total time of the analysis was approx. 5 min, with a significantly low eluent flow rate (0.5 mL min-1) compared to the literature. Additionally, a long cation-exchange column (250 mm × 4.0 mm) was used as reference. Depending on the total iron content in the sample, two plasma views were chosen, e.g., an attenuated axial (<2 g kg-1) and an attenuated radial. The standard addition method was performed for the method's accuracy studies, and the applicability was presented on three types of samples: sediments, soils, and archaeological pottery. This study introduces a fast, efficient, and green method for leachable iron speciation in both geological and pottery samples.

Physico-chemical and bacteriological characterization of surface waters for domestic use: Case of river waters in M'pody village (Anyama, Ivory Coast)

This study aimed at determining the physico-chemical and bacteriological quality of the waters of the rivers of the village M'pody in order to evaluate their impacts on the health of the populations. The methodology consisted of four water sampling campaigns in the Souman and Chibi rivers. Conventional physico-chemical parameters were determined by electrochemical and spectrophotometric methods. The microbiological analysis was carried out using the membrane filtration technique. The results showed that physico-chemical and bacteriological parameters are beyond the WHO recommendations. The noncompliance of this water is related to high levels of turbidity, conductivity, temperature and a high total iron content. For bacteriological parameters, this noncompliance is related to the presence of Escherichia coli, Tolerant Thermo Coliform, Total Coliform, Fecal Enterococci, Pseudomonas sp, Sulphite-Reducing Anaerobic) (SRA). Water of the rivers showed huge non-conformities related to physico-chemical and bacteriological parameters. This water was therefore unfit for human consumption. Thus, to minimize potential health risks, the adoption of hygiene measures in particular, a good purification of this water must be recommended to the populations in order to better preserve their health.

Iron removal from kaolin by oxalic acid using a novel pre-agitating and high-pressure washing technique

AbstractThis study reports a leaching process along with a novel physical pretreatment technique to remove iron impurities from kaolin using oxalic acid. A detailed examination of the physical properties of the minerals confirmed that almost none of the leaching-resistant iron oxides, specifically hematite, was completely interlocked with the clay minerals. Accordingly, a pre-agitating technique followed by high-pressure washing of the sample on a 600 μm sieve were applied. In this method, by discarding up to 20% of the raw material, &gt;54% of the total iron content of the raw kaolin was removed, and the iron content was reduced from 9.75% in the initial sample to &lt;6.00% in the passing fraction. Application of this pretreatment method rendered the leaching process successful at 97°C using 0.3 M oxalic acid solutions for 1 h under atmospheric pressure. Compared to the leaching of the raw material without pretreatment, which consumed at least 0.5 M oxalic acid over a minimum period of 2 h, the method used was very cost-effective and favourable, reducing the iron content of the product to &lt;1% and producing high-quality kaolin.

Screening of Eight Biofortified Rice Varieties Based on Total Content of Zinc and Iron as Well as Its Uptake by Caco-2 Cells: An In Vitro Cellular Model

Zinc (Zn) and iron (Fe) are two essential micronutrients that help the body to maintain its normal physiology and metabolism. Rice is the most important staple crop because it nourishes approximately half of the world’s population. The present study was intended to screen a set of eight rice varieties for total grain Zn and Fe content along with their uptake in differentiated Caco-2 cells. The total grain Zn and Fe content, as well as their bioaccessibility, was determined using inductively coupled plasma mass spectrometry (ICP-MS) techniques. The Caco-2 cell-based fluorescence assays and ferritin synthesis assays were used to determine Zn and Fe uptake, respectively. The transcriptional expression of micronutrient transporters has been analyzed in Caco-2 cells in response to the uptake of Zn and Fe from in vitro digested rice samples. The findings of this study revealed a comparative analysis of two prominent micronutrient contents in eight different varieties of polished rice. DRRDhan 48 (4.93 ± 0.10 mg/kg), Zinco Rice (4.65 ± 0.01 mg/kg), and Protozin (4.43 ± 0.03 mg/kg) were shown to have low phytate content, and high grain Zn content (32.49 ± 2.8, 27.9 ± 1.8, 26.7 ± 1.2 mg/kg, respectively), as well as significantly greater Zn uptake in differentiated Caco-2 cells. The outcome of this study provides an easy and convenient approach for the screening of food items or cereals based on micronutrient uptake.

Camptothecin Sensitizes Hepatocellular Carcinoma Cells to Sorafenib- Induced Ferroptosis Via Suppression of Nrf2

Sorafenib is a potent inducer of ferroptosis used to manage hepatocellular carcinoma (HCC). The ferroptosis induced by sorafenib activates the p62–Keap1–Nrf2 pathway. Abnormal activation of Nrf2 reduces sorafenib’s efficiency and ferroptosis action and induces sorafenib’s resistance. Consequently, our study tried to study the effect of a novel combination of sorafenib and Camptothecin (CPT, Nrf2 inhibitor) to improve sorafenib’s ferroptosis action and reduce sorafenib resistance in the treatment of HCC. We evaluated the efficacy of sorafenib and/or CPT using HepG2 and Huh7 cell lines. MTT assay evaluated the anti-proliferation effects. The combination index (CI) and dose reduction index (DRI) were calculated using Isobologram analysis. Malondialdehyde (MDA), total antioxidant capacity (TAC), iron concentration, glutathione peroxidase (GPX4), and glutathione reductase (GR) activity assays were used to determine the ferroptosis action of drugs. Western blot was used to investigate the expression of the implicated proteins. Bioinformatics tools were used to determine the correlation between these proteins. Finally, the HPLC technique is used to measure cellular drug uptake. Our results revealed a strong synergism between sorafenib and CPT. The synergetic combination significantly increases lipid peroxidation and iron concentration, decreases TAC, GPX4 and GR activity, and reduces the expression of both Nrf2 and SLC7A11. The downregulation of Nrf2 expression has a vital role in the reduction of resistance mediators to sorafenib against HCC cells like (p62, MT1G, and ABCG2) and improves the cellular uptake of sorafenib. The current study provided evidence that Nrf2 inhibition by CPT improves sorafenib’s sensitivity and reduces sorafenib’s resistance via the augmentation of sorafenib’s ferroptosis action.