Content Of Silage Research Articles

Nitrogen fertilisation method affects wheat silage ammonia-N and crude protein content

ABSTRACT This study aimed to evaluate the nitrogen fertilisation supply method on wheat silage fermentation parameters and nutritional value. Eighteen experimental plots were used in a randomised block design to assess the following treatments: (1) control (CON): crop without nitrogen fertilisation; (2) surface (SUR): 100 kg/ha N during the crop, applied in surface; (3) furrows (FUR): 100 kg/ha N during the crop, applied in furrows during the seeding. Whole plants were manually harvested at 5 cm height 83 days after the sowing. One experimental silo (PVC tubes) was produced for each agronomical plot and stored for 69 days. In general, N fertilisation did not affect silage pH and ammonia-N (NH3-N) content, but silos FUR had less NH3-N than those with SUR. Fertilisation increased the CP and DM content and reduced starch in the silage. FUR increased DM and reduced CP compared to SUR. Furthermore, N fertilisation increased non-protein nitrogen and decreased in vitro degradation of DM and OM. Fertilisation methods did not affect N fractions in silage or in vitro degradation. Therefore, N fertilisation, especially FUR, reduces NH3-N and CP and increases DM content of silage, but decreases in vitro DM and OM degradation.

Effects of Lactobacillus plantarum and Cellulase on Mixed Silages of Amaranthus hypochondriacus and Cornmeal: Fermentation Characteristics, Nutritional Value, and Aerobic Stability

In order to develop new feed resources, the aim of this study was to investigate the effects of moisture content, additives, and their interactions on the fermentation quality, aerobic stability, and in vitro digestibility of mixed silage of amaranth and cornmeal. The mass ratios of amaranth and cornmeal were 69:31, 76:24, and 84:16 for adjusting the moisture content of silage to 60% (W1), 65% (W2), and 70% (W3), respectively. The silage treatments included no additives (U), the addition of Lactobacillus plantarum (L), the addition of cellulase (E), and the addition of Lactobacillus plantarum + cellulase (M) mixed reagents. The results revealed that the pH and ammonia nitrogen (NH3-N/TN) ratios were significantly lower in W1 than in W2 and W3 (3.66,19.3 g kg−1 TN vs. 3.70, 3.70, 20.0 kg−1 TN, 25.1 kg−1 TN, p < 0.05). Moreover, dry matter (DM), organic matter (OM), in vitro dry matter digestibility (ivDMD), in vitro organic matter digestibility (ivOMD), and in vitro crude protein digestibility (ivCPD) significantly increased (p < 0.05). Meanwhile, the aerobic stability of mixed silage containing amaranth and cornmeal decreased with increasing water content. The aerobic stability of the L, E, and M treatment groups was improved by 15, 105, and 111 h, respectively, compared with that of the control group at W1. The pH and NH3-N/TN ratios were lower with the addition of E (E and M) than with the absence of E (U and L) (3.73, 20.1 g kg−1 DM vs. 3.64, 22.9 g kg−1 DM, p < 0.05). NDF and ADF were significantly lower with the addition of E than without the addition of E (598 g kg−1 DM, 145 g kg−1 DM vs. 632 g kg−1 DM, 160 g kg−1 DM, p < 0.05). However, CP, ivDMD, ivOMD, and ivCPD were significantly higher (p < 0.05). AA and NH3-N/TN were significantly lower (p < 0.05) with the addition of L (L and M) than without the addition of L (U and E). In conclusion, the best fermentation quality, in vitro digestibility, and aerobic stability of amaranth and cornmeal mixed silage treated with Lactobacillus plantarum + cellulase (M) were achieved at 60% water content. The present study confirmed the potential of amaranth as silage and its potential application for improving feed quality and animal performance.

The Effect of Fermented Rice Bran of The Silage of Cow’s Rumen with Different Storage Time on The Physical Quality, pH and Nutrient Content of Silage

This study, conducted from March to May 2023, aimed to assess the impact of fermented rice bran from cow rumen contents on silage quality, pH, and nutrient content with varying storage times. The experiment took place in Porame Village, Central Sulawesi Province, and Tadulako University's Feed Nutrition Laboratory. The study used molasses, rice bran, EM4, water, and cow rumen waste as materials. Employing a complete randomized design with a 2x3 factorial pattern, the study had 6 treatment combinations and 24 experimental units. Factors included fermented and unfermented rice bran ratios mixed with cow rumen content, and storage times (21, 25, and 30 days). Results from SPSS analysis indicated no significant interaction between fermented rice bran and storage time regarding physical quality and nutrient content. However, the pH of silage was significantly affected. Fermented rice bran (A2) led to a notable pH reduction compared to non-fermented rice bran (A1). In summary, this study concluded that while there's no substantial interaction effect on physical quality and nutrient content, pH measurements are impacted by the use of fermented rice bran.

Evaluation of Nutritional Content of Fermented Fish Visceral Silage using Sorbic Acid and Effective Microorganism (EM4)

Fish silage is a liquid product produced from whole or part of a fish processed by fermentation utilizing the activity of enzymes produced by microba. The fermentation process can be carried out chemically or biological. The purpose of this research is to determine the nutritional content of fish silage fermented using Sorbic Acid and EM4. This research uses an experimental method by mixing cleaned fish with 3% Sorbic Acid and 20% EM4. The highest protein content is found in fish silage fermented with Sorbic Acid, that is 55.05% followed by fat and carbohydrate content of 22.01% and 19.61%. Meanwhile the protein content of fish silage fermented with EM4 is 26.55%. Research results show that fish silage fermented with Sorbic Acid has the best performance by increasing the protein content of the silage.

Kualitas fisik dan fraksi serat silase rumput Gajah (Pennisetum purpureum) dengan penambahan molasses dan probiotik

The objective of the study was to analyze the use of molasses as an energy source of silage and probiotics on the physical/organoleptic quality of silage, fermentation characteristics, and NDF and ADF silage content. 15 kgs of Napier grass were packed in plastic silos for each treatment and stored for 21 days. The experiment used a completely randomized design (CRD) with 3 treatments and 4 replications. The treatment consisted of P0: control or the Napier grass silage, P1: Napier grass silage + 3% molasses, and P2: Napier grass silage + 3% molasses and EM4 probiotics. The data were tested by using an analysis of variance and the differences among treatment means were examined by the Least Significant Difference (LSD) test. The variables measured were organoleptic quality including color, odor, texture, presence of fungi, fermentation characteristics including pH, dry matter (DM), and fleigh value as well as the content of Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF). The results showed that the molasses and probiotics addition had no significant effect on the color, odor, and texture of the silage (P>0.05) but had a significant effect on the presence of fungi (P<0.01). Molasses and probiotics addition as fermentation additives reduced the pH value and increased the value of fleigh silage (P<0.05) but did not affect the increase of silage dry matter (P>0.05), decreased the NDF content (P<0.05) and had no significant effect on the ADF content. The conclusion of the research was the addition of molasses as an energy source, as well as a combination of molasses and EM4 probiotics can improve the fermentative quality of silage, by decreasing the pH value, increasing the fleigh value, and being able to reduce the NDF content of silage.

The Effect of Different Forms of Titanium Dioxide on the Yield, Chemical and Microbiological Parameters of Perennial Ryegrass (Lolium perenne L.) Herbage and Silage

The aim of this study was to evaluate the applicability of three forms of titanium dioxide (TiO2) and their effect on the yield, chemical and microbiological quality of perennial ryegrass herbage and silage. Two types of titanium dioxide nanoparticles (TiO2NPs) and a commercial product, labeled here as TiO2Com, were selected for the studies. The yield and chemical parameters of herbage did not improve significantly in response to the TiO2 treatment, in comparison to the control group (CONT). The crude protein content of silage was significantly lower in the TiO2Com-treated group than in the TiO2NPs2 group (117 vs. 129 g kg−1 dry matter (DM)). The use of water-soluble carbohydrates during fermentation was limited in the TiO2NPs2 and TiO2Com groups. The fermentation pattern was similar for each investigated group, and a significant difference in pH values was noted between the TiO2NPs2 (pH = 4.49) and CONT (pH = 4.68) group. Lactic acid concentration was the highest in the TiO2NPs1 group (94.8 g kg−1 DM), and the difference relative to the CONT group (83.2 g kg−1 DM) was statistically significant. It was concluded that TiO2 and its nanoparticles have the potential to improve the physicochemical and microbiological quality of herbage and silage.

Whole-plant flint corn silage inclusion in total mixed rations for pre- and postweaning dairy calves

Assuming that acetic acid plays a minor role in the development of ruminal epithelium of preweaning dairy calves, the fiber supply for growing calves has been neglected. More research has been done on including starch and nonfibrous carbohydrates in solid feed for preweaning calves. Accordingly, the fiber requirement of these calves is not well known, as diet recommendations vary greatly. Hence, elucidating the effects of including fiber from long particle sizes in the diet may be essential for helping calves overcome the transition challenge during weaning. Forty-five Holstein calves were used in a randomized block design, considering sex, birth date, and weight at 28 d of age, when the supply of the total mixed ration (TMR) with the inclusion of corn silage started. Three TMR with increasing whole-plant flint corn silage content (0, 10, or 20% on a dry matter basis) were compared: 0CS, 10CS, or 20CS, respectively. During the first 28 d of life, the calves were managed homogeneously and were fed 6 L/d of whole milk, a commercial calf starter pelleted, and water ad libitum. Next, the solid diet was changed to the respective solid feed treatment. Calves were gradually weaned from 52 to 56 d of age but were evaluated for an additional 14 d postweaning. Feed intake was measured daily, while body weight and metabolic indicators of intermediate metabolism were evaluated weekly. Ruminal fluid was collected at 6, 8, and 10 wk of age. Behavioral analysis was conducted on wk 7 (preweaning) and 10 (postweaning). There was a quadratic effect for dry matter intake from wk 7 to 10, with higher intake for the 10CS diet than the 0CS and 20CS diets. Consequently, the 10CS diet also promoted greater average daily gain at wk 8 and 9 compared with the 0CS and 20CS diets. However, the final body weight was not affected by the different solid diets. Silage inclusion in calves' diet positively affected time spent ruminating and chewing pre- and postweaning. Including 10% of whole-plant flint corn silage in the diets of young dairy calves is a strategy to increase total solid intake and decrease acidosis risk by increasing pH and ruminating activity around weaning.

Effects of Bacillus subtilis or Lentilactobacillus buchneri on aerobic stability, and the microbial community in aerobic exposure of whole plant corn silage.

This study aimed to evaluate the effects of Bacillus subtilis or Lentilactobacillus buchneri on the fermentation quality, aerobic stability, and bacterial and fungal communities of whole plant corn silage during aerobic exposure. Whole plant corn was harvested at the wax maturity stage, which chopped to a length of approximately 1 cm, and treated with the following: distilled sterile water control, 2.0 × 105 CFU/g of Lentilactobacillus buchneri (LB) or 2.0 × 105 CFU/g of Bacillus subtilis (BS) for 42 days silage. Then, the samples were exposed to air (23-28°C) after opening and sampled at 0, 18 and 60 h, to investigate fermentation quality, bacterial and fungal communities, and aerobic stability. Inoculation with LB or BS increased the pH value, acetic acid, and ammonia nitrogen content of silage (P < 0.05), but it was still far below the threshold of inferior silage, the yield of ethanol was reduced (P < 0.05), and satisfactory fermentation quality was achieved. With the extension of the aerobic exposure time, inoculation with LB or BS prolonged the aerobic stabilization time of silage, attenuated the trend of pH increase during aerobic exposure, and increased the residues of lactic acid and acetic acid. The bacterial and fungal alpha diversity indices gradually declined, and the relative abundance of Basidiomycota and Kazachstania gradually increased. The relative abundance of Weissella and unclassified_f_Enterobacteria was higher and the relative abundance of Kazachstania was lower after inoculation with BS compared to the CK group. According to the correlation analysis, Bacillus and Kazachstania are bacteria and fungi that are more closely related to aerobic spoilage and inoculation with LB or BS could inhibit spoilage. The FUNGuild predictive analysis indicated that the higher relative abundance of fungal parasite-undefined saprotroph in the LB or BS groups at AS2, may account for its good aerobic stability. In conclusion, silage inoculated with LB or BS had better fermentation quality and improved aerobic stability by effectively inhibiting the microorganisms that induce aerobic spoilage.

Storage time effects on whole-plant sorghum silage fermentation profile, aerobic stability and mycotoxin concentration

ABSTRACT This study aimed to evaluate the storage time effects on whole-plant sorghum silage nutrients fermentation profile, fermentation losses, mycotoxin concentrations and aerobic stability. Sixteen experimental silos (cylindrical bag with 32 cm i.d. and six kg of silage) were used in a completely randomised design to evaluate the following storage times: 45, 90, 180 and 360 days. Storage time linearly increased silage Ammonia-N, propionic and butyric acid concentrations. In addition, treatments had a quadratic effect on silage pH and concentrations of lactic and acetic acids. Storage time did not affect fermentation losses, dry matter recovery and zearalenone concentration. However, storage time linearly decreased silage dry matter concentration and linearly increased deoxynivalenol silage content. Aflatoxin and fumonisin were not identified in silage samples. Storage time linearly increased silage pH regardless of evaluation period, and temperature, 24 h after aerobic exposure. Thus, the storage time of whole-plant sorghum silage affects the fermentation profile, has no effect on fermentation losses, increases deoxynivalenol content and reduces silage aerobic stability.

Effects of small-scale silo types and additives on silage fermentation and bacterial community of high moisture alfalfa on the Qinghai-Tibetan Plateau

Alfalfa is cultivated throughout the world, but its preservation remains challenging to small farms in cold climates. This study aimed to investigate the effects of small-scale silo types and additives on the silage quality of high moisture alfalfa on the Qinghai-Tibetan Plateau. Alfalfa with moisture of about 80% was ensiled in small farm bucket silos (FBS) or wrapped bale silos (WBS) at ambient temperature (5–15 °C) for 180 days. At ensiling, forage was treated without any additive (CK) or with a chemical additive (SB; a mixture of sodium benzoate, potassium sorbate, and sodium nitrite) or a lactic acid bacteria (LAB) inoculant (PBB; a mixture of Lactiplantibacillus plantarum, Bifidobacterium brevis and Pediococcus pentosaceus). Among the CK silages, higher (P < 0.05) acetic acid levels, bacterial diversity indices and relative abundance of L. plantarum were found in WBS vs FBS. Overall, silage treated with SB had the highest crude protein (CP) content, the lowest amylase-treated neutral detergent fiber (aNDF) and acid detergent fiber (ADF) contents and dry matter (DM) loss. Compared with CK, treatments with SB and PBB increased (P < 0.05) lactic acid content and decreased (P < 0.05) final pH value and butyric acid content of silage. In addition, treatment with PBB reduced (P < 0.05) the relative abundance of Lactilactobacillus sakei and increased (P < 0.05) the similarity of silage samples from different silos. There was negligible difference in fermentation quality of silage occurred between the types of silos. However, small WBS tended to increase the relative abundance of L. plantarum for more desirable silage fermentation. Application of additives at ensiling enhanced the preservation of silage nutrients with low DM loss. Based on the above results, small WBS were recommended for producing high-quality alfalfa silage on the Qinghai-Tibetan Plateau.

The Effect of Temulawak (Curcuma xhantorrhiza) Dose on Tofu Dreg Plus Complete Feed Silage With Methane Relative Mitigation: In-Vitro

Temulawak is an antimicrobial herb that can preserve the proteins in the feed from deamination and decay during the silage period. Tofu dregs are a feed ingredient that is difficult to preserve because they have high water content and protein. Temulawak powder may decrease deamination during the silage process because it has the ability as an antimicrobe and protein binder. Temulawak can reduce methane emissions by diminishing the number of protozoa that serve as hosts for methanogenic archaea. Temulawak with a harvest age of 3 months after planting, then dried at 65°C to constant weight, grounded, and tested on mix ration silage at doses of 0%, 0.25%, 0.5%, 0.75 %, and 1% from feed dry matter (DM). The experiment was carried out four times using a complete random design and in vitro silage was evaluated in the following 14 days. The results indicated that adding temulawak would reduce ammonia production with p&lt;0.01, significantly increase the crude protein content of silage P&lt;0.05 and decrease rumen ammonia P&lt;0.01. The treatment increased total volatile fatty acid (VFA) production P&lt;0.01), and reduced methane level P&lt;0.01. The methane relative to dry matter (DM), organic matter, and total gas revealed a significant decrease with P&lt;0.05. The 0.25% treatment level had the lowest total methane concentration and relative. Concluded that the optimal dose of temulawak addition to complete feed plus tofu dreg silage is 0.25% from DM.

Fibrolytic Enzymes Increase Fermentation Losses and Reduce Fiber Content of Sorghum Silage

Abstract Fiber digestibility is a key-point of forage usage in ruminant production systems. The present study aimed to evaluate the effect of fibrolytic enzyme blend on whole-plant sorghum silage fermentation profile, fermentative losses, chemical composition, in vitro degradation, and aerobic stability. It used fifty experimental silos (plastic bucket, 28 cm i.d. and 25 cm in height) in a blocked randomized design to evaluate the following fibrolytic enzyme levels: 0, 150, 300, 450, and 600 mL per ton of dry matter (DM). Enzymes had 300 U/mL of xylanase and 300 U/mL of cellulase. Enzymes linearly increased acetic acid, branched-chain organic acids, and ethanol concentration of silage. Although there was no treatment effect on silage DM recovery over the ensiling process, enzyme levels linearly increased gas, effluent, and total losses. Intermediary levels of enzymes (from 312 to 342 mL/ton DM) decreased fiber content (NDF and ADF), whereas improved NFC silage content. Besides, enzymes did not affect DM and NDF degradation and tended to linearly reduce acid detergent fiber in vitro degradation. The enzymes addition linearly decreased silage temperature after aerobic exposure. However, there was no treatment effect on silage pH after aerobic exposure also during the time that silage remained with a temperature lower than 2°C above environmental temperature. Thus, fibrolytic enzymes reduce fiber content, promote a heterolactic fermentation, and reduce silage temperature after aerobic exposure. However, this increases fermentative losses and has no positive effect on in vitro degradation and the time of aerobic stability.

Prolonged drought regulates the silage quality of maize (Zea mays L.): Alterations in fermentation microecology.

Prolonged drought stress caused by global warming poses a tremendous challenge to silage production of maize. Drought during maize growth and development resulted in altered micro-environment for silage fermentation. How fermentation of silage maize responds to moisture scales remains uncharted territory. In this research, Maize water control trials were conducted and the silage quality and microbial community of drought-affected maize were determined. The results showed that drought stress significantly reduced the dry matter but increased root-to-shoot ratio, soluble sugar and malonaldehyde content in maize. Before fermentation, the crude protein, crude ash and acid detergent fiber contents were significantly increased but the ether extract content was decreased under drought. The crude protein and acid detergent fiber were significantly decreased in the drought affected group after fermentation. Furthermore, water stress at maize maturity stage greatly reduced the number of total bacteria in silage fermentation but increased the proportion of the lactobacillus and lactic acid content of silage. Drought stress alters the microbial ecosystem of the fermentation process and reconstitutes the diversity of the bacterial community and its metabolites. This study provides a theoretical basis for the study of changes in silage fermentation as affected by abiotic stresses.

Effect of lactobacilli inoculation on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage.

Alfalfa (Medicago sativa L.) silage is one of the major forages with high protein for ruminants. The objective of this study was to investigate the effects of lactobacilli inoculants on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage. Wilted alfalfa (35% dry matter) was inoculated without (control) or with Lactobacillus coryniformis, Lactobacillus casei, Lactobacillus plantarum, and Lactobacillus pentosus and ensiled for 7, 15, and 60 days. Silage inoculated with L. pentosus was superior to L. coryniformis, L. casei, L. plantarum in improving the fermentation quality of alfalfa silage, as indicated by the lowest ammonia nitrogen content and silage pH during ensiling. There was minor difference in water soluble carbohydrates content among all silages, but L. pentosus inoculants was more efficient at using xylose to produce lactic acid, with lower xylose content and higher lactic acid content than the other inoculants. Compared with the control, L. pentosus inoculants did not affect true protein content of silage, but increased the proportions of buffer soluble protein and acid detergent soluble protein. The L. pentosus inoculants reduced the bacterial diversity In alfalfa silage with lower Shannon, Chao1, and Ace indices, and promoted relative abundance of lactobacillus and decreased the relative abundance of Pediococcus compared with the control. As well as L. pentosus inoculants up-regulated amino acid, carbohydrate, energy, terpenoids, and polypeptides metabolism, and promoted lactic acid fermentation process. In summary, the fermentation quality and nutrient preservation of alfalfa silage were efficiently improved by inoculated with L. pentosus.

Effect of rice bran and microorganism as additives in pearl millet silage

ABSTRACT This study aimed to evaluate rice bran (RB) and microbial inoculation effects on pearl millet silage fermentative losses, fermentation profile, nutrients recovery, chemical composition, in vitro degradation and aerobic stability. Seventy-two experimental silos (PVC buckets with 28 cm diameter and 25 cm height) were used to evaluate nine treatments, from a 3 × 3 factorial arrangement: (I) levels of RB: 0, 100 and 200 g/kg as-fed; and (II) microbial inoculation: (1) Control (CON): no inoculation; (2) LPPP: inclusion 1.04 × 105 colony forming units (CFU) of Lactobacillus plantarum and 1.04 × 105 CFU of Pediococcus pentosaceus per g; and (3) LB: inclusion 1.6 × 105 CFU of Lactobacillus buchneri per g. The silage microbial inoculant inclusion had no effect on silage, regardless of RB addition. Rice bran increased silage pH and decreased ammonia-N, propionic, butyric and branched-chain fatty acids content. Rice bran decreased gas losses and recovery of DM, OM, CP and ADF, whereas increased digestible DM recovery. The addition of RB reduced OM, NDF, ADF and ADL silage content, and increased silage DM, CP and EE, and NDF degradation. Therefore, RB increases digestible DM recovery, and improves silage fermentation and nutritional value.

Lactobacillus buchneri inoculation compared to chitosan and facultative heterofermentative lactic acid bacteria improves sugarcane silage conservation

AbstractThe present study aimed to evaluate the effects of chitosan instead of microbial inoculants on fermentation profile, losses, chemical composition, in vitro degradation, and aerobic stability of sugarcane silage (SS). Forty experimental silos (PVC tubes with 28 cm i.d., 25 cm height) were used in a randomized block design to evaluate the following treatments: (I) Control (CON): SS with no additive; (II) LB: SS ensiled with 5.0 × 105 colony forming units (CFU) of Lactobacillus buchneri (NCIM 40788)/g as-fed; (III) LPPA: SS ensiled with 1.6 × 105 CFU of L. plantarum and 1.6 × 105 CFU of Pediococcus acidilactici/g as-fed; and (IV) Chitosan (CHI): SS ensiled with 6 g/kg dry matter (DM) of chitosan. Microbial inoculation of SS reduced (P ≤ 0.05) silage pH relative to CON and CHI treatment. The LPPA decreased ammonia-nitrogen and LB decreased (P ≤ 0.05) ethanol content and increased acetic acid content relative to other treatments. The LPPA-silos had higher (P ≤ 0.05) gas losses and lower (P ≤ 0.05) DM recovery than other treatment silos. Consequently, LPPA reduced (P ≤ 0.05) DM and non-fibre carbohydrates and increased (P ≤ 0.05) neutral detergent fibre (NDF) silage content compared to other treatments. Treatments did not affect (P ≥ 0.212) DM and NDF in vitro degradation and silage pH after aerobic exposure. However, LB reduced silage temperature after aerobic exposure. Thus, LB reduces alcoholic fermentation and improves SS aerobic stability. Inoculation of LPPA reduces DM recovery and negatively affects SS chemical composition. Although CHI positively affects SS conservation relative to CON, it shows higher gas losses and decreased SS temperature after aerobic exposure compared to LB.

Fermentation Quality, In Vitro Digestibility, and Aerobic Stability of Total Mixed Ration Silage in Response to Varying Proportion Alfalfa Silage.

Simple SummaryEnsiling a total mixed ration (TMR) allows longer storage times for thoroughly mixed feed. In our previous study, we found that alfalfa silage could directly provide an acidic environment in fresh TMR and had a function comparable to that of lactic acid bacterial inoculants in improving fermentation quality during ensiling. However, the specific percentage of silage addition to the TMR has not been evaluated. In this study, we explored the effects of different proportions of alfalfa silage on the fermentation quality, in vitro digestibility, and aerobic stability of TMR silage and discussed the results in terms of the chemical composition of the feed. We found that the gradual development of an acidic environment during the ensiling process, which can be achieved with 40% alfalfa silage addition, can improve aerobic stability. However, the addition of 60% or 20% alfalfa silage might cause lower aerobic stability or clostridial spoilage in TMR silage. Our findings provide a more in-depth understanding of the effects of silage components on TMR silage and may guide farmers to apply a proper silage composition in rations to avoid the loss of feed value during storage or feeding stages.This study aimed to evaluate the effects of different proportions of alfalfa silage on the fermentation quality, in vitro digestibility, and aerobic stability of total mixed ration (TMR) silage. Three TMRs were prepared with different silage contents on a fresh matter basis: (1) 60% alfalfa silage (AS60), (2) 40% alfalfa silage (AS40), and (3) 20% alfalfa silage (AS20). The lactic acid in AS60 did not increase after 30 days of ensiling (p > 0.05). Butyric acid was detected in the AS20 group after 14 days of ensiling. The AS60 group showed significantly higher in vitro dry matter digestibility than the AS20 group (p < 0.05). The aerobic stability of TMR silage gradually increased with a decreasing percentage of alfalfa silage (p < 0.05). Unlike AS60, which directly gained an acidic environment from the alfalfa silage, AS40 developed a stable acidic environment during ensiling and further improved aerobic stability. However, when the percentage of alfalfa silage was reduced to 20%, a risk of clostridial spoilage occurred in the TMR silage. Therefore, the addition of 40% alfalfa silage to TMR is optimal and could achieve both good fermentation quality and considerable resistance to aerobic deterioration in TMR silage.

Effects of LAB Inoculants on the Fermentation Quality, Chemical Composition, and Bacterial Community of Oat Silage on the Qinghai-Tibetan Plateau.

Lactic acid bacteria (LAB) have been proposed for the control of undesirable fermentation and, subsequently, aerobic deterioration due to their ability to produce antimicrobial metabolites in silage mass. To investigate the effect of specific LAB on the silage fermentation characteristics and bacterial community composition of oat in cold regions, silages were treated without (control) or with three LAB strains (LB, Lentilactobacillus buchneri; nLP, low temperature tolerant Lactiplantibacillus plantarum; pLP, phenyllactic acid-producing Lactiplantibacillus plantarum), and then stored at ambient temperature (−2.63 ± 5.47–14.29 ± 5.48 °C) for 30, 60, and 90 days. Compared with control, inoculation of LAB decreased the final pH value, butyric acid content, ammonia-N of total N and dry matter loss of silage. Treatments with nLP and pLP increased (p < 0.05) lactic acid content, whereas LB increased (p < 0.05) acetic acid content of silage. Lactiplantibacillus and Leuconostoc dominated in the silages with relative abundance of 68.29–96.63%. A prolonged storage period enhanced the growth of Leuconostoc in pLP-treated silage. In addition, pLP increased (p < 0.05) the aerobic stability of silage as compared with nLP. In conclusion, inoculation of LAB improved silage fermentation and/or delayed aerobic deterioration by shifting bacterial community composition during ensiling. Phenyllactic acid-producing Lactiplantibacillus plantarum as an inoculant exhibited potential for high quality silage production.

PH, KANDUNGAN BAHAN KERING DAN SIFAT FISIK SILASE LIMBAH KOL DENGAN BERBAGAI PENAMBAHAN LEVEL DEDAK PADI

Cabbage waste has the potential to be used as an alternative feed for livestock if the feed is processed sustainably. This study aims to analyze the determine the best level of use of rice bran in making cabbage waste silage seen from physical properties, pH and dry matter content of silage. This study used a completely randomized design with 4 treatments and 4 replications. The treatments were A0 (cabbage waste 100%), A1 (cabbage waste 75% + rice bran 25%), A2 (cabbage waste 50% + rice bran 50%), A3 (cabbage waste 25% + rice bran 75%). The parameters measured include pH, odor, color, texture, presence of mushrooms and dry matter content. The results showed that the substitution of various levels of rice bran on cabbage waste had no significant effect (P 0.05) on the pH, color, texture and presence of mushrooms, had a significant effect (P 0.05) increased the aroma value, and had a very significant effect ( P 0.01) increased the dry matter content of the silage. The best treatment in this study is A3 (cabbage waste 25%, rice bran 75%) because it can increase the odor and dry matter content.

The degradation of residual pesticides and the quality of white clover silage are related to the types and initial concentrations of pesticides.

In order to understand the degradation of different residual pesticides of white clover silage and their influence on silage quality, three commonly used orchard pesticides with different concentrations were added to the white clover and fermented for 90 days. The results showed that the degradation rate of cypermethrin and its toxic degradation product 3-phenoxybenzoic acid (3-PBA) was the highest after silage, at different concentrations, both were 100%. The degradation rate of Tebuconazole and chloropyridine was 72.47–80.27% and 47.76–64.82%, of which 3,5,6-trichloro-2-pyridinol (TCP) content, poisonous toxic degradation product, increased 0.0525–0.253 mg·kg−1. The residues of beta-cypermethrin and tebuconazole had reached safety standards after silage. As compared with the control, the contents of lactic acid, acetic acid, and propionic acid increased in the treated samples. The higher concentrations of three pesticides all significantly reduced the lactic acid content of silage (p<0.05). Pesticides had different effects on the nutritional components of white clover silage. Conclusively, silage is a potential way to expand the utilization of covering plants in orchards.