Soil Nutrient Content Research Articles

Spider waste enhances soil nutrient content, soil respiration, and plant growth

Abstract Predators can alter the movement of nutrients through ecosystems by depositing waste products following predation. Whilst the benefits of predator waste for large predators (e.g. bears) or dense accumulations of predators (e.g. seabirds on islands) seem clear, less is known about whether smaller, solitary predators can have measurable effects on local ecosystem processes. In separate experiments with web‐building and wandering spiders, we tested if the presence of predators affected soil nutrient content, soil respiration, soil microbial communities, and plant growth. In the first experiment with black widow spiders, total nitrogen and nitrate were not affected by spider presence, but ammonia and phosphorus were higher from soil under the edge of the spider web than soil away from the spider. Soil respiration and plant growth were both higher in soil collected from under the spider retreat compared with soil collected away from the spider web. In a second experiment with wolf spiders, we tested for interactions between spiders and soil microbial communities. There were positive effects of wolf spider presence on all soil nutrients and there were interactions between spiders and soil type (i.e. field‐collected versus autoclaved) for total carbon, total nitrogen, nitrate, and pH. Spider presence and soil type also affected soil respiration and spider presence had a large effect on the composition of the microbial community of the soil. There were also positive effects of wolf spider presence on plant biomass and plant height, with a significant interaction between spiders and soil type for plant height. Overall, our results show that two spiders with different life histories (i.e. web‐building and wandering) both have significant positive effects on plant growth through the deposition of their waste products. These effects may occur through the direct deposition of nutrients and changes in soil microbial communities. Although, further work is needed to resolve these interactions. Read the free Plain Language Summary for this article on the Journal blog.

Critical levels and fertility classes of soils with high-activity clay in the Brazilian semi-arid region

ABSTRACT Soil fertility evaluation is important for adopting conservation management and adequate nutrient supply. The objective of this study was to identify critical levels and soil fertility classes using the boundary line method for rainfed crops (corn, cowpea and sabiá [Mimosa caesalpiniifolia]) in the Brazilian semi-arid region. A database of 226 soil fertility analyses of samples from the 0.00-0.20 m soil layer, and corn, cowpea and sabiá yields from Ceará State was used to generate interpretation classes (at 80 and 95 % of maximum yield). In a scatter plot, soil nutrient concentrations (x-axis) and relative crop yields (y-axis) were correlated, and the border points fitted to a quadratic model. Proposed interpretation classes were classified as very low, low, adequate, high and very high, except for Na + , whose adequate class was considered tolerable. Generated models showed coefficients of determination (R 2 ) for the chemical properties ranging from 0.54 to 0.92. Based on the interpretation classes, the critical level was determined as 6.3 for pH, 10.8 g dm -3 for OM, 20.9 mg dm -3 for P, 81 mg dm -3 for K, 55 mmol c dm -3 for Ca 2+ , 24 mmol c dm -3 for Mg 2+ and 8 mg dm -3 for S-SO 4 2- . Interpretation classes for soils with high-activity clay in the Brazilian semi-arid region were superior to those in the reference literature. Boundary line method established fertility classes and critical levels for soil chemical properties in more than one crop, using the concept of relative yield.

Reduction of tobacco alkaloid bioaccumulation in pea shoots: a comparative study of biochar derived from cow dung and maize straw

Tobacco alkaloids in tobacco-cultivated soils pose potential risks for succeeding crops, due to their allelopathy and toxicity. Effects of biochar on the dissipation of tobacco alkaloids in soil-crop systems remain poorly understood. In this study, a 40-day pot experiment was conducted to explore the effect of cow dung biochar (CDBC) and maize straw biochar (MSBC) on the uptake of nicotine and nornicotine by pea (Pisum sativum L.) and their dissipation in an agricultural soil. The results revealed that the bioaccumulation of nicotine and nornicotine by pea shoots in the soils added with CDBC and MSBC at 1.5% and 3.0% significantly decreased by 46.97–79.13% and 33.64–71.59%, respectively. CDBC more effectively decreased the uptake and bioaccumulation of nicotine and nornicotine by pea shoots than MSBC due to the higher soil pH and nutrient content. In addition, the enhanced relative abundances of soil nicotine-degrading bacteria belonging to the genera Arthrobacter and Gemmatimonas also contributed to the decreasing uptake of nicotine by pea plants. The decreased bioavailability in the soils due to the increased adsorption was the key factor for the reduced bioaccumulation of tobacco alkaloids. This study provides guidance to protect subsequent crops in tobacco-cultivated soil from tobacco alkaloids with biochar.

Extreme rainfall events eliminate the response of greenhouse gas fluxes to hydrological alterations and fertilization in a riparian ecosystem

Riparian ecosystems are essential carbon dioxide (CO2) sources, which considerably promotes climate warming. However, the other greenhouse gas fluxes (GHGs), such as methane (CH4) and nitrous oxide (N2O), in the riparian ecosystems have not been well studied, and it remains unclear whether and how these GHG fluxes respond to extreme weather, fertilization and hydrological alterations associated with reservoir management. Here, we assessed the impacts of hydrological alterations (i.e., flooding frequency) and fertilization (nitrogen and/or phosphorus) induced by human activities (hydroengineering construction and agricultural activities) on GHG fluxes, and further investigated the underlying mechanisms in two contrasting years (normal vs. extreme rainfall years) in a reservoir riparian zone dominated by grasses. The significant combined effects of extreme rainfall events and human activities (hydrological alterations and fertilization) on the GHGs were observed. Continuous flooding reduced CO2 emissions by 24% but increased CH4 emissions by ∼4 times in a normal rainfall year. In addition, nitrogen fertilization promoted CO2 emissions by 37%. However, these phenomena were not observed in the year with extreme rainfall events, which made the flooding levels homogeneous across the treatments. Furthermore, we found that CO2 fluxes were driven by the soil moisture, nutrient content, aboveground biomass, and root carbon content, while CH4 and N2O fluxes were merely driven by the soil properties (pH, moisture, and nutrient content). This study provides valuable insights into the crucial role of extreme rainfall events, hydrological alteration, and fertilization in regulating GHG fluxes in riparian ecosystems, as well as supports the integration of these changes in GHG emission models.

Applying kitchen compost promoted soil chrysene degradation by optimizing microbial community structure

Chrysene, as a high molecular weight polycyclic aromatic hydrocarbon (PAH), has become an important factor in degrading soil quality and constraining the safe production of food crops. Compost has been widely used to amend contaminated soil. However, to date, the main components of kitchen compost that enhance the biodegradation of chrysene in the soil remain unidentified. Thus, in this study, the enhancing effect and mechanisms of kitchen compost (KC) and kitchen compost-derived dissolved organic matter (KCOM) on chrysene removal from soil were investigated through cultivation experiments combined with high-throughput sequencing technology. Additionally, the key components influencing the degradation of chrysene were identified. The results showed that KCOM was the main component of compost that promoted the degradation of chrysene. The average degradation rate of chrysene in 1% KC- and 1% KCOM-treated soil increased by 27.20% and 24.18%, respectively, at different levels of chrysene pollution compared with the control treatment (CK). KC and KCOM significantly increased soil nutrient content, accelerated humification of organic matter, and increased microbial activity in the chrysene-contaminated soil. Correlation analyses revealed that the application of KC and KCOM optimized the microbial community by altering soil properties and organic matter structure. This optimization enhanced the degradation of soil chrysene by increasing the abundance of chrysene-degrading functional bacteria from the genera Bacillus, Arthrobacter, Pseudomonas, Lysinibacillus, and Acinetobacter. This study provides insight into the identification of key components that promote chrysene degradation and into the microbial-enhanced remediation of chrysene-contaminated soil.

Effects of Applying Organic Amendments on Soil Aggregate Structure and Tomato Yield in Facility Agriculture

Amendment significantly improves soil structure and promotes crop growth. To combat soil degradation and low crop yields in facility agriculture, it is crucial to study the optimal application rate of amendments. This study analyzed the effects of biochar, vermicompost, and mineral-source potassium fulvic acid on the stability of aggregate structure, soil nutrient content, and tomato yield in cambisols, providing a theoretical basis for improving the soil quality of plastic greenhouses in Southern China. A pot experiment on tomato cultivation was carried out in yellow-brown soil in plastic greenhouses. The experiment included eight treatments: 1% biochar (B1); 3% biochar (B3); 5% biochar (B5); 3% vermicompost (V3); 5% vermicompost (V5); 0.1% mineral-source potassium fulvic acid (F1); 0.2% mineral-source potassium fulvic acid (F2); and the control condition without adding soil amendments (CK). The results showed that the biochar and vermicompost treatments effectively reduced soil bulk density and increased total soil porosity. Compared to the control, treatments with soil amendments significantly increased soil pH and had different effects on soil nutrients: F2 showed the most significant improvement in the content of available nitrogen, available phosphorus, and available potassium, with an increase of 133.33%, 834.59%, and 74.34%, respectively; B3 treatment had the highest increase in dissolved organic carbon (DOC), while B5 treatment had the highest organic matter content. Compared to the CK, the particle size of the biochar treatment was mainly 0.053~0.25 mm, while the V3, F1, and F2 mainly occurred with a particle size > 0.25 mm; and V3 has the best aggregate stability. Biochar, vermicompost, and mineral potassium fulvic acid can all promote tomato yield, with the F2 and V3 treatments having a yield increase effect of over 30%. Furthermore, Pearson’s correlation analysis showed a highly significant positive correlation between geometric mean diameter (GMD) and mean weight diameter (MWD), water-stable macroaggregate content (R0.25), and a positive correlation between alkaline-dissolved nitrogen, available phosphorus, dissolved organic carbon content, and aggregate stability indicators. Adding 0.2% mineral-source potassium fulvic acid optimizes cambisols’ properties, enhances aggregate formation and stability, boosts tomato yield, and shows great application potential.

Changes in Nutrient Surpluses and Contents in Soils of Cereals and Kiwifruit Fields

Knowledge of nutrient surpluses in soils is critical to optimize nutrient management and minimize adverse environmental effects. We investigated the nutrient surpluses in soils in two regions over 25 years (1992 to 2017) in the south Loess Plateau, China. One region has cereals as the main crop, whereas in the other region, the main cereal crops was changed to kiwi orchards. The inputs of nitrogen (N), phosphorus (P), and potassium (K) increased rapidly (by 74%, 77%, and 103% from 1992 to 2017 in the cereal region; and by 91%, 204%, and 368% in the kiwifruit region), while the nutrient outputs were relatively stable, which resulted in increasing nutrient surpluses (the annual averaged surpluses of N, P, and K were 178, 62, and 12 kg ha−1 y−1 for the cereal region; and 486, 96, and 153 kg ha−1 y−1 for the kiwifruit region) and lower nutrient use efficiency (NUE). The higher N surplus in the orchard-dominated region caused high nitrate N accumulation (3071 kg N ha−1 of 0–5 m in 11–20 y in the kiwifruit orchard) in deeper soil profiles. Similarly, high P and K surpluses in the orchard-dominated region increased soil available P and K. This highlights that comprehensive measures should be taken to control nutrient surpluses, which will help balance nutrient inputs and outputs and minimize nutrient losses in intensive horticultural crop systems.

Differential effects of pH on cadmium accumulation in Artemisia argyi growing in low and moderately cadmium-contaminated paddy soils

BackgroundPhytoremediation is affected by physical and chemical properties of the soil such as soil pH, moisture, and nutrient content. Soil pH is a key element influencing Cd bioavailability and can be easily adjusted in agricultural practices. The soil pH level may relate to the effectiveness of phytoremediation; however, this has not been extensively investigated. In the current study, we evaluated the effect of Cd contamination level (0.56 and 0.92 mg/kg) and soil pH (5, 6, and 7) on Cd accumulation and allocation in Artemisia argyi, a fast-growing perennial crop.ResultsOur results indicated that higher soil Cd concentrations reduce A. argyi biomass, and the loss of the root mass was particularly significant. Higher soil pH decreased Cd content in stems and roots of A. argyi cultivated in moderately Cd-polluted soil, and increased Cd content in stems and roots of the plant grown in low Cd-polluted soil. Higher soil pH decreased the percentage of Cd distributed in the soluble fraction and cell walls and increased the percentage of Cd in the organelles of leaf cells for moderate soil Cd levels. The bioconcentration and translocation factor exceeded 4.0 and 1.0, respectively, across all tested treatments, indicating that A. argyi is a promising candidate for phytoremediation. Notably, the effects of soil pH on Cd accumulation and subcellular distribution in A. argyi differed between low and moderately Cd-contaminated soils.ConclusionAdjustments to soil pH based on the degree of Cd contamination can enhance Cd extraction by A. argyi, thereby reducing the overall remediation cycle of cadmium-polluted paddy fields of South China.Graphical

The Comprehensive Effects of Biochar Amendments on Soil Organic Carbon Accumulation, Soil Acidification Amelioration and Heavy Metal Availability in the Soil–Rice System

In recent years, biochar (BC) and biochar-based soil amendments (CSAs) have been widely used in agriculture and the environment. In the present study, a two-rice-season field study was conducted to explore the comprehensive effects of applying BC (1%) and CSA (0.5% and 1%) on soil organic carbon accumulation, soil acidification amelioration and heavy metal availability in a soil–rice system. The results show that soil pH was increased by 0.5–1.7 units and 0.3–1.0 units, respectively, in the early rice season and late rice season treated by the amendments compared with CK. Soil organic contents were increased by 18–30% in the early rice season and by 15–25% in the late rice season in the amended treatments. In addition, soil available phosphorus contents were largely increased as a result of BC and CSA addition. Soil CaCl2 extractable heavy metals (Cd, Ni, Cu and Zn) were simultaneously decreased by BC or CSA amendments. In addition, Cd contents in early rice grain and late rice grain were significantly reduced by 25–48% and 52–83% in amended treatments, while Zn contents were generally not affected. The uptake of Cu and Ni was also decreased by BC and CSA. This study demonstrates that biochar application alone or combinates with inorganic amendments (limestone, sepiolite and potassium dihydrogen phosphate) can significantly improve soil properties and nutrient content and decrease the heavy metal (especially for Cd and Ni) uptake and accumulation from soil to rice grain, where the combination application is more effective.

The effects of livestock grazing on physicochemical properties and bacterial communities of perlite-rich soil.

Livestock grazing has been proposed as a cost-effective way to reclaim post-mining lands. It can enhance soil fertility and biodiversity, but its impacts on soil quality and microbial communities vary across soil types. Moreover, waste from grazing raises concerns about pathogens that could pose risks to animal and human health. This study investigated the effects of grazing on post-mining perlite-rich soil in central Thailand. A comparative analysis of soil physicochemical properties and bacterial diversity was conducted between grazed and ungrazed sites. Bacterial diversity was assessed using 16S amplicon sequencing. The perlite-rich soil was found to be sandy, acidic, and to have low nutritional content. Grazing significantly improved the soil texture and nutrient content, suggesting its potential as a cost-effective reclamation strategy. The 16S metagenomic sequencing analysis revealed that microbial communities were impacted by livestock grazing. Specifically, shifts in the dominant bacterial phyla were identified, with increases in Firmicutes and Chloroflexi and a decrease in Actinobacteria. Concerns about increased levels of pathogenic Enterobacteriaceae due to grazing were not substantiated in perlite-rich soil. These bacteria were consistently found at low levels in all soil samples, regardless of livestock grazing. This study also identified a diverse population of Streptomycetaceae, including previously uncharacterized strains/species. This finding could be valuable given that this bacterial family is known for producing antibiotics and other secondary metabolites. However, grazing adversely impacted the abundance and diversity of Streptomycetaceae in this specific soil type. In line with previous research, this study demonstrated that the response of soil microbial communities to grazing varies significantly depending on the soil type, with unique responses appearing to be associated with perlite-rich soil. This emphasizes the importance of soil-specific research in understanding how grazing affects microbial communities. Future research should focus on optimizing grazing practices for perlite-rich soil and characterizing the Streptomycetaceae community for potential antibiotic and secondary metabolite discovery. The obtained findings should ultimately contribute to sustainable post-mining reclamation through livestock grazing and the preservation of valuable microbial resources.

Rubber-Based Agroforestry Ecosystems Enhance Soil Enzyme Activity but Exacerbate Microbial Nutrient Limitations

Agroforestry ecosystems are an efficient strategy for enhancing soil nutrient conditions and sustainable agricultural development. Soil extracellular enzymes (EEAs) are important drivers of biogeochemical processes. However, changes in EEAs and chemometrics in rubber-based agroforestry systems and their mechanisms of action are still not fully understood. Distribution of EEAs, enzymatic stoichiometry, and microbial nutrient limitation characteristics of rubber plantations under seven planting patterns (RM, rubber monoculture system; AOM, Hevea brasiliensis-Alpinia oxyphylla Miq; PAR, Hevea brasiliensis-Pandanus amaryllifolius Roxb; AKH, Hevea brasiliensis-Alpinia katsumadai Hayata; CAA, Hevea brasiliensis-Coffea Arabica; CCA, Hevea brasiliensis-Cinnamomum cassia (L.) D. Don, and TCA, Hevea brasiliensis-Theobroma Cacao) were analyzed to investigate the metabolic limitations of microorganisms and to identify the primary determinants that restrict nutrient limitation. Compared with rubber monoculture systems, agroforestry ecosystems show increased carbon (C)-acquiring enzyme (EEAC), nitrogen (N)-acquiring enzyme (EEAN), and phosphorus (P)-acquiring enzyme (EEAP) activities. The ecoenzymatic stoichiometry model demonstrated that all seven plantation patterns experienced C and N limitation. Compared to the rubber monoculture system, all agroforestry systems exacerbated the microbial limitations of C and N by reducing the vector angle and increasing vector length. P limitation was not detected in any plantation pattern. In agroforestry systems, progression from herbs to shrubs to trees through intercropping results in a reduction in soil microbial nutrient constraints. This is primarily because of the accumulation of litter and root biomass in tree-based systems, which enhances the soil nutrient content (e.g., soil organic carbon, total nitrogen, total phosphorus, and ammonium nitrogen) and accessibility. Conversely, as soil depth increased, microbial nutrient limitations tended to become more pronounced. Partial least squares path modelling (PLS-PM) indicated that nutrient ratios and soil total nutrient content were the most important factors influencing microbial C limitation (−0.46 and 0.40) and N limitation (−0.30 and −0.42). This study presented novel evidence regarding the constraints and drivers of soil microbial metabolism in rubber agroforestry systems. Considering the constraints of soil nutrients and microbial metabolism, intercropping of rubber trees with arboreal species is recommended over that of herbaceous species to better suit the soil environment of rubber plantation areas on Hainan Island.

Amelioration of Soil Fertility by the Application of Different Fruit Waste Composts

The soil health status of Bangladesh decreased day by day, particularly organic matter and major nutrient content. The organic matter content in most of the agricultural soil in Bangladesh is <2%, which indicates poor soil health condition. In Bangladesh, almost every day, an enormous amount of waste material is produced from different kinds of seasonal and unseasonal fruit’s peels. Lack of proper recycling methods, most of these wastes are thrown away in drains, cannels, rivers, etc. which indicates a major threat to environmental safety. Composts derived from the wastes of different fruit’s peels can be an excellent source of organic matter and essential nutrients for soil health improvement. Therefore, the present study was carried out to evaluate the changes in physicochemical properties and nutrient contents of experimental soil treated with compost prepared from different types of fruit’s waste. The compost was prepared accordingly using different types of fruits waste and then the collected compost was thoroughly mixed with the soil and kept for six months. The experimental treatment consisted of three organic composts (jackfruit peel compost, mango peel compost, and banana peel compost) and three rates of application (1000, 1500, and 2000 kg ha-1). The experiment was arranged in a factorial completely randomized design and replicated thrice. The analyzed data showed that, the physicochemical properties and nutrient contents of experimental soil varied significantly with the use of different types of fruit’s wastes compared to no waste added soil, except in the case of particle size. Soil mixed with mango peel compost at a rate of 2000 kg ha-1 resulted in a higher available N content (2520 mg kg-1) and available P content (42.56 mg kg-1), whereas the available K content (219.88 mg kg-1), EC (1.55 dS m-1) and CEC (1209.56 meq 100g-1) were found higher in soil treated with banana peel compost of 2000 kg ha-1. On the other hand, soil treated with jackfruit peel compost of 1500 kg ha-1 showed the higher available Ca content (1533.33 mg kg-1) and the jackfruit peel compost of 2000 kg ha-1 resulted in the higher organic carbon (1.98%) and organic matter (3.41%). The findings of this experiment revealed that, compost prepared from different types of fruit peels can be an excellent source of organic matter and nutrients and among the tested composts, mango peel compost was better to improve the physiochemical properties of soil and supply sufficient nutrients for plant growth.

Effects of Soil Nutrient Restoration Aging and Vegetation Recovery in Open Dumps of Cold and Arid Regions in Xinjiang, China

Open-pit coal mining inevitably damages the soil and vegetation in mining areas. Currently, the restoration of cold and arid open-pit mines in Xinjiang, China, is still in the initial exploratory stage, especially the changes in soil nutrients in spoil dumps over time. Dynamic remote sensing monitoring of vegetation in mining areas and their correlation are relatively rare. Using the Heishan Open Pit in Xinjiang, China, as a case, soil samples were collected during different discharge periods to analyze the changes in soil nutrients and uncover the restoration mechanisms. Based on four Landsat images from 2018 to 2023, the remote sensing ecological index (RSEI) and fractional vegetation cover (FVC) were obtained to evaluate the effect of mine restoration. Additionally, the correlation between vegetation changes and soil nutrients was analyzed. The results indicated that (i) the contents of nitrogen (N), phosphorus (P), potassium (K), and organic matter (OM) in the soil increased with the duration of the restoration period. (ii) When the restoration time of the dump exceeds 5 years, N, P, K, and OM content is higher than that of the original surface-covered vegetation area. (iii) Notably, under the same restoration aging, the soil in the artificial mine restoration demonstration base had significantly higher contents of these nutrients compared to the soil naturally restored in the dump. (iv) Over the past five years, the RSEI and FVC in the Heishan Open Pit showed an overall upward trend. The slope remediation and mine restoration project significantly increased the RSEI and FVC values in the mining area. (v) Air humidity and surface temperature were identified as key natural factors affecting the RSEI and FVC in cold and arid open pit. The correlation coefficients between soil nutrient content and vegetation coverage were higher than 0.78, indicating a close and complementary relationship between the two. The above results can clarify the time–effect relationship between natural recovery and artificial restoration of spoil dumps in cold and arid mining areas in Xinjiang, further promoting the research and practice of mine restoration technology in cold and arid open pits.

Effects of nutritional stress on soil fertility and antioxidant enzymes of rice in different growth periods.

Stress in plants denotes the detrimental impact of alterations in external environmental conditions on regular plant growth and development. Plants employ diverse mechanisms to mitigate or evade nutritional stress-induced damage. In order to investigate the physiological response mechanism of plants to nutritional stress and assess its impact on soil nutrient content and antioxidant enzyme activity in rice, a field experiment was conducted applying five treatments: control, nitrogen (N) deficiency, phosphorus (P) deficiency, potassium (K) deficiency, and full fertilization. Rice leaf and soil samples were concurrently gathered during both the vegetative and reproductive growth stages of rice. Analysis was conducted on soil N, P, and K levels, as well as leaf antioxidant enzyme activities, to investigate the impact of nutrient stress on rice antioxidant enzymes and soil fertility. The research findings indicate that full fertilization treatment enhanced the agronomic properties of the soil compared to the control treatment. In the N-deficiency treatment, reactive oxygen species (ROS) levels increased by 16.53-33.89% during the reproductive growth period compared to the vegetative growth period. The peroxidase (POD) activity decreased by 41.39% and superoxide dismutase (SOD) activity increased by 36.22% under K-deficiency treatment during the reproductive growth period compared to the vegetative growth period. Consequently, applying N and P fertilizer during the vegetative growth period can decrease membrane lipid peroxidation levels by 7.34-72.53%. The full fertilization treatment markedly enhanced rice yield compared to other treatments and increased the Nitrogen activation coefficient (NAC) and Phosphorus activation coefficient (PAC) in the soil, while decreasing the PAC. Elevating NAC levels can stimulate the activity or content of PRO, MDA, and RPS during the vegetative growth stage, whereas in the reproductive growth stage, it will decrease the content of ROS, PRO, and MDA. This data offers valuable insights and theoretical support for nutritional stress research.

The Effects of Acid-Modified Biochar and Biomass Power Plant Ash on the Physiochemical Properties and Bacterial Community Structure of Sandy Alkaline Soils in the Ancient Region of the Yellow River

The application of biochar can effectively enhance soil organic matter (SOM) and improve soil structure. Biomass power plant ash (BPPA) is also rich in essential nutrients for plants, with similar carbon content. Considering production cost and agricultural waste recycling, it is beneficial to apply BPPA to improve soil fertility and quality. However, it remains unclear whether its ameliorative effects surpass those of biochar in alkaline soils. In the study, we set up seven pot experiments of faba beans in sandy alkaline soils from the ancient region of the Yellow River, including the controls (CK), different amounts of acid-modified BPPA (A1, A2, A3), and the same amounts of acid-modified biochar (B1, B2, B3), to compare their effects on soil physiochemical properties and bacterial community structure. The results indicate that the application of both biochar and BPPA can improve soil physiochemical properties. At the same dosage, the biochar application outperformed BPPA treatment in terms of soil physical properties such as bulk density (BD), maximum water-holding capacity (FC), and soil capillary porosity (SP2). Conversely, BPPA treatment displayed advantages in chemical properties such as readily oxidizable organic carbon (ROOC), total nitrogen (TN), alkaline nitrogen (AN), available phosphorus (AP), available potassium (AK), and electrical conductivity (EC). All the treatments enhanced the richness and diversity of bacterial communities, increasing the relative abundance of eutrophic groups such as Bacteroidota and Firmicutes while decreasing that of oligotrophic groups like Actinobacteriota. BPPA also increased the relative abundance of Proteobacteria, while the opposite was observed for biochar. Correlation analysis showed that the environmental factors such as soil pH, EC, TN, AK, SOM, and SP2 emerged as primary factors influencing the bacterial community structure of alkaline soils, significantly affecting their diversity and abundance. Among them, SP2 and SOM were the dominant physical and chemical factors, respectively. Overall, the application of both acid-modified BPPA and biochar can enhance the physiochemical properties of sandy alkaline soils, while the application of BPPA is superior for improving soil nutrient content and enhancing bacterial community structure. The study explores the potential mechanisms through which the application of acid-modified BPPA affects soil characteristics and microbial features, providing new insight into developing optimizing fertilization strategies for enhancing soil quality in the ancient region of the Yellow River.

Effect of Sod Production on Physical, Chemical, and Biological Properties of Soils in North and South China

Lawns play a vital role in urban development, but the impact of sod production on soil properties has always been controversial. In this study, we examined the physical, chemical, and biological properties of sod production bases across different regions and years [including northern China (2.5, 3, 5, 6, 8, 10, 12 years), referred to as N-2.5, N-3, etc., and southern China (3, 10, 11, 14, 17 years), referred to as S-3, S-10, etc.], with tall fescue and Kentucky bluegrass planted in the north and bermudagrass or creeping bentgrass planted in the south. Sod production was found to increase soil bulk density while reducing porosity and field capacity, but these effects did not consistently intensify with longer production periods. Except for available phosphorus and available potassium, other soil nutrients (total carbon, total nitrogen, organic matter, alkali-hydrolyzable nitrogen, etc.) were either unaffected or increased at certain time points (S-11, S-14). Prolonged sod production (S-10, S-17) also boosted microbial content. In northern regions, organic matter and total nitrogen were the key factors influencing microbial community structure, whereas in southern regions, alkali-hydrolyzable nitrogen, electrical conductivity, available potassium, and organic matter were most influential. We also found that crop rotation, sand mulching, and deep plowing could enhance soil nutrient content and microbial activity in sod production.

NATIVE MYCORRHIZAE FROM ETHIOPIA IMPROVE TREE GROWTH AND SEEDLINGS SURVIVAL CONTRIBUTING TO THE GREEN LEGACY PROGRAM

<p><strong>Background</strong>: A rapid production of tree seedlings in nurseries with a high survival rate after transplanting is important to respond to the current demand for programs of restoration of arid environments by forestation. The low level of seedlings’ survival and establishment, caused by low moisture and nutrient content of soils, has been a bottleneck to reaching the target of the forest national restoration in Ethiopia of last years. It is suggested that, inoculation with root-associated plant growth promoting microorganisms could help to ameliorate this scenario and also respond to the Ethiopia's Green Legacy Program. <strong>Objective</strong>: To assess the potential of inoculation with arbuscular mycorrhizal fungi (AMF) native to Ethiopia to improve the survival and growth of trees that could be used in afforestation programs in Ethiopia. <strong>Methodology</strong>: The study was carried out in three stages: (1) soil samples associated with roots of selected acacia species (T<sub>1</sub>-AMF of<em> A. abyssinica</em>, T<sub>2</sub>-AMF of<em> A. seyal</em>, T<sub>3</sub>-AMF<em> </em>of <em>A. tortilis </em>and<em> </em>T<sub>4</sub>-Control) were collected of highland and lowland areas from Ethiopia, (2) Sorghum (<a href="https://www.researchgate.net/publication/342429051_Evaluation_of_sorghum_Sorghum_bicolor_L_Moench_variety_performance_in_the_lowlands_area_of_wag_lasta_north_eastern_Ethiopia"><em>Sorghum bicolor</em> (L.), provided by the Melkasa Agricultural Research Center-(MARC) served as a trap plant for </a>the AMF consortium multiplication and (3) plant growth promotion by AMF was assessed throughout inoculations of seedlings of<em> </em><em>Delonix regia</em> (Hook.) Raf.,<em> Sesbania grandiflora </em>(L.)<em>, Cassia fistula </em>L<em>., </em>and<em> Azadirachta indica </em>A. Juss., trees<em>. </em><strong>Results</strong>: All inoculated seedlings showed significantly greater responses in all growth and mycorrhizal parameters over the non-inoculated trees. Consortium T<sub>2</sub>-AMF associated to roots of<em> A. seyal </em>from lowlands of Batu, showed significantly greater responses in all plant growth and mycorrhizal parameters over the AMF inoculums associated to other tree species evaluated. Significant and positive correlations were found between mycorrhizae and plant-growth parameters<em>. </em><strong>Implications:</strong> Our results suggest that inoculation with native arbuscular mycorrhizal fungi indigenous from Ethiopia has the potential to significantly enhance survival and growth rates of tree seedlings. This could thereby advance national reforestation goals and addressing challenges in seedling establishment in arid environments. <strong>Conclusion</strong>: The potential for growth promotion and establishment of tree seedlings evidenced, implies that further efforts should be directed towards the in-mass production of AMF-based inoculants, particularly associated with <em>A. seyal</em> roots.</p>

Exotic mangrove Laguncularia racemosa litter input accelerates nutrient cycling in mangrove ecosystems.

Exotic plant litter presents different chemical and physical properties relative to native plant litter and alters ecosystem processes and functions that may facilitate exotic plant dispersal. However, these effects are largely unknown, especially within wetland ecosystems. This study examines whether introducing litter from theexotic mangrove Laguncularia racemosa could result in (1) accelerated community litter decomposition rates and increased nutrient cycling rates and (2)microbial community structure changes in the invaded areas. A single decomposition experiment using litterbags was conducted to examine the short-term effects of L. racemosa litter in the native mangrove forest ecosystem. The soil nutrients and microbial communities of Rhizophora stylosa, L. racemosa, and mixed forests were also compared to explore the long-term cumulative effects of L. racemosa litter in native ecosystems. The results indicated that L. racemosa has lower-quality leaf litter than R. stylosa and a significantly faster decomposition rate. This may result from changes in the soil microbial community structure caused by L. racemosa leaf litter input, which favors the decomposition of its own litter. Both the short-term and cumulative effect experiments demonstrated that L. racemosa leaf litter significantly increased the relative abundance of microbes related to litter decomposition, such as Proteobacteria and Bdellovibrionota, and enhanced the alpha diversity of soil fungi, thus creating a microbial environment conducive to L. racemosa leaf litter decomposition. Moreover, the accumulation of soil nutrients was lower under L. racemosa than under R. stylosa over several years. This may be related to the more rapid growth of L. racemosa, which causes soil nutrient absorption and storage within the plant tissues, thereby reducing the soil nutrient content. Inputting exotic mangrove L. racemosa leaf litter reduced the soil blue carbon content, potentially adversely affecting global climate change. L. racemosa may employ a unique strategy to lower soil nutrient levels in native mangroves based on its low-quality leaf litter, thereby weakening the competitive ability of native plants that are intolerant to low-nutrient conditions and enhancing its own competitive advantage to further spread into these areas. In summary, the input of exotic L. racemosa leaf litter accelerates nutrient cycling in local mangroves.

Unveiling the Hidden Responses: Metagenomic Insights into Dwarf Bamboo (Fargesia denudata) Rhizosphere under Drought and Nitrogen Challenges.

Dwarf bamboo (Fargesia denudata) is a crucial food source for the giant pandas. With its shallow root system and rapid growth, dwarf bamboo is highly sensitive to drought stress and nitrogen deposition, both major concerns of global climate change affecting plant growth and rhizosphere environments. However, few reports address the response mechanisms of the dwarf bamboo rhizosphere environment to these two factors. Therefore, this study investigated the effects of drought stress and nitrogen deposition on the physicochemical properties and microbial community composition of the arrow bamboo rhizosphere soil, using metagenomic sequencing to analyze functional genes involved in carbon and nitrogen cycles. Both drought stress and nitrogen deposition significantly altered the soil nutrient content, but their combination had no significant impact on these indicators. Nitrogen deposition increased the relative abundance of the microbial functional gene nrfA, while decreasing the abundances of nirK, nosZ, norB, and nifH. Drought stress inhibited the functional genes of key microbial enzymes involved in starch and sucrose metabolism, but promoted those involved in galactose metabolism, inositol phosphate metabolism, and hemicellulose degradation. NO3--N showed the highest correlation with N-cycling functional genes (p < 0.01). Total C and total N had the greatest impact on the relative abundance of key enzyme functional genes involved in carbon degradation. This research provides theoretical and technical references for the sustainable management and conservation of dwarf bamboo forests in giant panda habitats under global climate change.

Farmland Degradation in the Czech Republic: Drivers and Barriers of Mitigation Strategies in Agricultural Soils

ABSTRACTIn line with Sustainable Development Goal (SDG) 15.3, which aims to restore degraded land and soil, and the priorities of the Common Agriculture Policy (CAP) to mitigate environmental issues caused by intensive agriculture, understanding the factors influencing farmers' adoption of soil conservation practices (SCPs) is crucial. However, there is still a limited understanding of these specific factors, particularly in the context of the Czech Republic. This study investigates the perceptions and determinants influencing the adoption of SCPs among farmers in the Czech Republic. We analyzed 358 randomly selected farm households using probit and multivariate probit models. Our results show that a significant number of farmers perceive soil degradation as an important problem, attributing it to factors such as low soil nutrient content, declining soil humus, water and wind erosion. The results of our analysis showed positive associations for perceived effectiveness and profitability, indicating that farmers tend to adopt practices such as minimum tillage, mulching, mixed cropping, cover cropping, and continuous soil cover when these methods are perceived to be effective and profitable. Conversely, negative associations are found for perceptions of soil degradation and education, suggesting potential barriers to adoption with higher levels of perceived soil degradation and education. The study also highlights the complex interplay of information sources on adoption, with both positive and negative trends. In light of these findings, we propose recommendations, including that awareness campaigns should be tailored to address perceptions of soil degradation, and that the use of peer networks and information dissemination from research institutions can bridge the gap between scientific recommendations and on‐farm practices. Policy makers and agricultural extension services should work together to develop targeted strategies that take into account regional differences in the factors influencing adoption and ultimately promote widespread adoption of SCPs.