Dietary Fiber Content Research Articles

Effects of Supplementation of Insoluble Dietary Fiber Obtained from Cinnamon Spent Bark Waste on the Performance of Nile Tilapia (<em>Oreochromis niloticus</em>) Fingerlings

Dietary fiber supplementation has proven benefits on fish health and growth. Cinnamon spent bark waste is the cinnamon bark residue after oil distillation. It is a rich source of insoluble dietary fiber with very low contents of soluble dietary fiber. This study aimed to investigate the potential of using water-extracted insoluble fiber from cinnamon spent bark waste as a functional ingredient in the diets of Oreochromis niloticus fingerlings. Four experimental diets were prepared by replacing a commercial feed with extracted dietary fiber at 0 (control), 0.5%, 1%, and 1.5% levels. Fingerlings of O. niloticus were assigned to the four experimental diets and the feeding trial was conducted for 12 weeks. The results revealed that the weight gain, specific growth rate, feed conversion ratio, Fulton’s condition factor, and survival rate of fish were not significantly different among the experimental groups. Fiber supplementation at 1.5% significantly increased (p<0.05) the total aerobic bacteria population in feces, whereas the coliform counts in feces at 0.5% and 1% fiber supplementation were significantly lower (p<0.05) than the control. There was a significant increase (p<0.05) in red blood cells count at 0.5% fiber supplementation. Moreover, insoluble dietary fiber supplementation significantly (p<0.05) increased the white blood cell counts in blood. Results suggested that insoluble fiber supplementation affected gut microbial populations and blood parameters of O. niloticus fingerlings. However, further investigations on gut microbiology and hematology are needed to ensure the use of insoluble dietary fiber from cinnamon spent bark waste as a functional ingredient in the diets of O. niloticus fingerlings.

High-Value Compounds in Papaya By-Products (Carica papaya L. var. Formosa and Aliança): Potential Sustainable Use and Exploitation.

Food waste is a global and growing problem that is gaining traction due to its environmental, ethical, social, and economic repercussions. Between 2022 and 2027, the worldwide papaya market is expected to have a huge increase, meaning a growth in organic waste, including peels and seeds. Thus, this study evaluated the potential use of peels and seeds of two mature papaya fruits as a source of bioactive compounds, converting these by-products into value-added products. Proximate analysis (AOAC methods), mineral content (ICP-MS), free sugars (HPLC-ELSD), fatty acid composition (GC-FID), vitamin E profile (HPLC-DAD-FLD), and antioxidant activity (DPPH and FRAP assays) were evaluated. Both by-products showed high total protein (20-27%), and dietary fiber (32-38%) contents. Papaya peels presented a high ash content (14-16%), indicating a potential application as a mineral source. 14 fatty acids were detected, with α-linolenic acid (30%) as the most abundant in the peels and oleic acid (74%) in the seeds. Both by-products showed high antioxidant activity. Papaya by-products display great potential for industrial recovery and application, such as formulation of new functional food ingredients.

The balance of evidence on 100% juice & health: A systematic umbrella review of meta-analyses

Fruit and vegetable intakes are major modifiable determinants of risk for non-communicable disease(1), yet intake levels remain low(2) and multiple barriers (cost, access, perishability, preparation skills) exist(3,4). 100% fruit and vegetable juices contain key micronutrients and bioactive compounds (5–7) and may help circumvent these barriers to consumption(6,7). However, their role in dietary guidelines and models of healthy eating remains controversial due to their free sugars and reduced dietary fibre content, relative to whole fruits and vegetables(6,7). Therefore, we conducted a systematic umbrella review of systematic literature reviews (SLRs) with meta-analyses assessing the relationships between 100% juice consumption and human health outcomes. Four databases (Medline, Cochrane Library, EMBASE, and CINAHL) were systematically searched for SLRs with meta-analyses of human prospective cohort, case-control, and intervention studies examining the relationship between 100% juice and any health outcome through to 20th October 2022. Screening (Covidence), quality (GRADE)(8), risk of bias (ROBIS)(9) and content overlap (corrected covered area(10)) tools were applied, and extracted data were narratively synthesised. The protocol was pre-registered (PROSPERO) and conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklists. 15 SLRs on 100% fruit juice including 51 primary meta-analyses, 6 dose-response analyses, and 87 sub-analyses were eligible for inclusion. No eligible studies on vegetable juice were found. Included studies represented data from almost 2 million subjects with a range of doses (50-1200mL/day) and timeframes (hours to years). Significant improvements in health outcomes were found in 19.6% of included meta-analyses (blood pressure, flow-mediated dilation, IL-6, c-reactive protein, and stroke mortality), and increased disease risks were found in 5.9% of included meta-analyses (CVD mortality, prostate cancer, and type II diabetes). The remainder (74.5%) found no significant difference (blood lipids, weight, liver function, metabolic markers, colorectal and breast cancers, and multiple inflammatory markers). The ROBIS quality assessment rated nine SLRs as low risk of bias, three as unclear and three as high. Using GRADE, confidence in the body of evidence ranged from very low (27 primary and 79 secondary meta-analyses) to low (19 primary and 13 secondary meta-analyses), and medium (4 primary and one secondary meta-analyses.) Findings show 100% juice consumption has limited risks of harm and some potential benefits, over a broad range of doses, including some that are relatively high, and time periods. The positive associations between 100% juice consumption and specific health outcomes relevant to population health may be explained by multiple mechanisms, including the vitamin, mineral, and bioactive contents. The balance of evidence suggests that 100% may have a neutral or beneficial place in general, population-level dietary guidelines.

Legume Fortified Instant Noodles: Optimization of Product Formulation and Processing Parameters using Response Surface Methodology

Cereal-pulses-based fortification has been attempted to improve the protein and dietary fibre content of instant noodles without altering the overall acceptability. Response surface methodology (RSM) was employed to optimize the process parameters and level of ingredients for manufacturing instant noodles. The effect of maize flour and split yellow pea flour fortification (6-18% each), the addition of vital wheat gluten (0.4-2.0%), and steaming time (1.5-7.5 min) on cooking loss, water uptake, volume expansion, cooking time, overall acceptability, L*, a* and b* values were investigated using the central composite rotatable design (CCRD). Results showed that maize flour and split yellow pea flour significantly (p≤0.05) affected cooking loss, water uptake, volume expansion, cooking time, overall acceptability, L*, a*, and b* values at linear levels. Vital wheat gluten had a significant effect on cooking loss, overall acceptability, L*, and a* value. Steaming time had a significant interactive effect with split yellow pea flour and vital wheat gluten on the responses. Incorporation of 18.0% maize flour, 11.25% split yellow pea flour, and 0.4% vital wheat gluten in formulation with 5.87 minutes of steaming time resulted in optimum quality instant noodles. A 20% increase in protein and a 50% increase in fiber content was observed in optimized instant noodles.

A whole food, plant-based randomized controlled trial in metastatic breast cancer: feasibility, nutrient, and patient-reported outcomes

PurposeQuality of life (QOL) is among the most important outcomes for women with metastatic breast cancer (MBC), and it predicts survival. QOL is negatively impacted by cognitive impairment, fatigue, and weight gain. We assessed whether a whole food, plant-based (WFPB) diet-promoting weight loss is feasible and might improve QOL.MethodsWomen with MBC on stable systemic treatments were randomized 2:1 to 1) WFPB dietary intervention (n = 21) or 2) usual care (n = 11) for 8 weeks. Participants attended weekly education visits and consumed an ad libitum WFPB diet (3 prepared meals/day provided). Patient-reported outcomes and 3-day food records were assessed at baseline and 8 weeks. The effects of WFPB diet on changes in outcomes were assessed by analysis of covariance model controlling for baseline.Results20 intervention and 10 control participants completed the trial. Intervention participants were highly adherent to the WFPB diet (94.3 % total calories on-plan). Intervention group nutrient intakes changed significantly including dietary fat (35.8 % to 20.4 % percent calories from fat, p < 0.001) and fiber content (12.7 to 30.8 g fiber/1000 kcal, p < 0.001). Perceived cognitive function (FACT-Cog total + 16.1; 95 % confidence interval [CI] = 0.8–31.7; p = 0.040) and emotional well-being (FACT-B emotional well-being subscale + 2.3; CI = 0.5–4.1; p = 0.016) improved in the WFPB versus the control group. Fatigue, measured by the BFI, improved within the WFPB group for fatigue severity (M = 4.7 ± 2.5[SD] to 3.7 ± 2.3, p = 0.047) and fatigue at its worst (5.8 ± 2.8 to 4.4 ± 2.4, p = 0.011).ConclusionsSignificant dietary changes in this population are feasible and may improve QOL by improving treatment-related symptoms. Additional study is warranted.Trial Registration: ClinicalTrials.gov identifier: NCT03045289. Registered 7 February 2017.

Investigation of the sensory quality, nutritional value and antioxidant capacity of flakes prepared using various pigeon pea-based flours

Pigeon pea (Cajanus cajan (L.)) is a potential source of nutritional and antioxidant compounds. Germination proved to improve the nutritional quality and antioxidant capacity of pigeon peas. The previous author’s study showed that pigeon pea flour prepared by NaCl elicitation before germination exhibits significantly higher antioxidant capacity and functional properties than that prepared without elicitation or germination. The study aimed to examine the sensory quality, nutrition value, and antioxidant capacity of flakes formulated using non-germinated pigeon pea flour, pigeon pea sprout flour, and NaCl-elicited pigeon pea sprout flour. The potential of the flours to be developed as commercial flakes was also determined using oat-based commercial flakes as a comparator. The NaCl-elicited pigeon pea flour-based flakes showed a higher quality score of both texture and overall qualities than the other pigeon pea flour-based flakes and the commercial ones. Regarding nutritional value, flakes formulated using NaCl-elicited pigeon pea sprout flour also showed better nutritional value, indicated by the lowest fat content and highest soluble, insoluble, and total dietary fiber contents. The highest values of total phenolics content (TPC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP) were also observed in the NaCl-elicited pigeon pea sprout flour-based flakes, even though its DPPH radical scavenging activity was not significantly different to the commercial flakes. These results have significant consequences for developing legume-based flakes with higher levels of dietary fibers and antioxidant potential, and lower fat content.

Effect of Oatmeal on TNF-alfa of Blood of White Rats Exposed to Motor Vehicles Fumes

Background: Air pollution due to motor vehicle emissions causes several serious health problems including respiratory problems. Fumes exposure activated alveolar macrophages and epithelial cells release mediators such as LTB4, TNF-α, and IL-8. TNF-α are known induced acute inflammation and tissue damage. Oats are plant with high dietary fiber content and nutritional value. Oats are contain of antioxidant compounds source such as tocopherols and avenanthramides. Therefore, this research aims to determine the effect of oatmeal on TNF-α of white rats exposed to motor vehicle fumes. Methods: This research used true experiment design with Randomized Post-Test Only Control Group design using 16 white male rats divided into three groups: control group only was given standard fed (K1), second group was given standard fed and exposed with motor vehicle fumes (K2), third group was given standard fed mixed with 10 gr of oatmeal and exposed with motor vehicle fumes (K3) for ten days. After ten days of the treatment, blood of white rats from all groups will be taken intracardial then measured TNF- α levels. Results: Statistical test analyzed using SPSS Software 23. Oatmeal has an effect to decrease TNF-α levels (P&lt;0.001). Conclusion: This study conclude that oatmeal can help reduce TNF-α levels when exposed to motor vehicle fumes.

Bioaccessibility of Phenolic Compounds, Resistant Starch, and Dietary Fibers from Australian Green Banana during In Vitro Digestion and Colonic Fermentation.

Green bananas contain a substantial amount of resistant starch (RS), dietary fiber (DF), and phytochemicals, which exhibit potent antioxidant capabilities, primarily attributable to the abundance of polyphenols. The objective of this study was to assess the variations in the contents and bioaccessibility of RS, DF, and phenolic compounds in three types of Australian green bananas (Cavendish "Musa acuminata", Ladyfinger "Musa paradisiaca L.", and Ducasse "Musa balbisiana"), along with their antioxidant capacities, and the production of short-chain fatty acids (SCFAs) following in vitro simulated gastrointestinal digestion and colonic fermentation. The studied cultivars exhibited significant levels of RS, with Ladyfinger showing the greatest (49%). However, Ducasse bananas had the greatest DF concentration (38.73%). Greater TPC levels for Ladyfinger (2.32 mg GAE/g), as well as TFC and TTC (0.06 mg QE/g and 3.2 mg CE/g, respectively) in Cavendish, together with strong antioxidant capacities (DPPH, 0.89 mg TE/g in Cavendish), have been detected after both intestinal phase and colonic fermentation at 12 and 24 h. The bioaccessibility of most phenolic compounds from bananas was high after gastric and small intestinal digestion. Nevertheless, a significant proportion of kaempferol (31% in Cavendish) remained detectable in the residue after colonic fermentation. The greatest production of SCFAs in all banana cultivars was observed after 24 h of fermentation, except valeric acid, which exhibited the greatest output after 12 h of fermentation. In conclusion, the consumption of whole green bananas may have an advantageous effect on bowel health and offer antioxidant characteristics.

Development of Gluten-Free Bread from Brown Rice-Potato Starch Composite Flour Incorporated with Red Seaweed Kappaphycus alvarezii powder

Gluten free diets and lifestyles are rapidly increasing in popularity mainly due to the increase in consumer awareness about gluten related disorders and gluten intolerances. With increasing demand, the food industry strives to develop new food products that are gluten-free and able to be consumed by those who suffer from these conditions. The objective of this study was to develop a gluten-free bread from brown rice-potato starch composite flour incorporated with red seaweed Kappaphycus alvarezii (KA) powder. KA powder, ranging from 2% to 10%, was added, and the physical properties of the gluten- free bread were assessed. Sensory evaluation was conducted using two methods, the descriptive and hedonic tests. The amount of seaweed significantly (p&lt;0.05) influenced the loaf volume, with F5 (10%), the highest concentration of seaweed, having the highest reduction of volume of 15.38% compared to the control F0 (without seaweed). With increasing seaweed concentration, the brightness and yellowness of each bread decreased. The texture profiles of KA-incorporated bread formulations were also affected with the firmness of bread being the most significant change. The descriptive test showed that F5 (10%) had the highest intensity of seaweed effect which affected the colour, texture, aroma, taste and aftertaste of the bread. The hedonic test showed that the most acceptable formulation was F1 (2%). From these formulations, the best formulation F1 (2%) was chosen for further nutrient analysis where it was compared to the control (without seaweed). It was shown that F1 had significantly (p&lt;0.05) higher moisture, ash, fat, and dietary fibre content, but lower carbohydrate and protein content compared to F0 (without seaweed). In conclusion, the formulation of gluten-free KA- incorporated brown rice-potato starch bread had an impact on the bread texture, colour, volume, and nutrient content.

The Effects of Polod (Arenga undulatifolia) Powder towards Physicochemical Properties and Sensory Attributes of Chicken Patties

Meat products have high biological value protein and essential nutrients needed for human sustenance, but it is deficient in complex carbohydrates like dietary fibre. Thus, various strategies have been explored to increase the dietary fibre content in meat products because plants are potentially rich sources of dietary fibres and bioactive compounds. Arenga undulatifolia, commonly known as polod, is a member of the family Arecaceace and grows natively in Borneo. This study examined how incorporating polod powder produced from the stem pith of Arenga undulatifolia affects nutritional, physicochemical, and sensory aspects of chicken patties. The polod powder showed comparable functional properties to other plant powders. The chicken patties were formulated as control samples F0 (0% polod powder), F1 (2% polod powder), F2 (4% polod powder), F3 (6% polod powder). Adding 4% polod powder significantly increased (p &gt; 0.05) the patty’s protein, ash, lipid, crude fibre, dietary fibre, and energy contents. Increasing the level of polod powder considerably lowered the moisture content (p &lt; 0.05). The chewiness of the patties decreased significantly (p &lt; 0.05) at 4% and 6% polod powder levels with no significant changes in other textural parameters (p &gt; 0.05). Compared to the control sample (F0), the chicken patties with polod powder showed greater cooking yield and lower cooking loss. Compared to other formulations, F1 was most liked by sensory panellists. In conclusion, polod powder is potentially useful as a functional ingredient in the production of chicken patties.

An Overview of Date (Phoenix dactylifera) Fruits as an Important Global Food Resource.

Dates are the fruits of the date palm belonging to the Arecaceae family; they comprise over 2500 species and 200 genera and constitute an essential part of the daily diet worldwide, with beneficial nutritional, health, and economic values. Several varieties of date palm (Phoenix dactylifera) fruit exist globally, especially in hot and humid regions. This review is an overview of date palms as a significant global food resource, including their historical significance, nutritional composition, cultivation practices, economic importance, and health benefits. The historical journey of dates goes back to ancient civilizations where they were revered for their richness in essential nutrients and natural sweetness. Today, dates are a vital crop in arid regions, contributing substantially to the agricultural economy and livelihoods of communities. This paper further explores the cultivation techniques employed to enhance date production. Furthermore, the nutritional composition of dates is analyzed in detail, highlighting their high content of vitamins, minerals, dietary fibers, and antioxidants. These attributes make dates a delicious treat and a valuable nutritional component, offering numerous health benefits. The potential health effects, including improved digestion, enhanced cardiovascular health, and increased energy levels, are discussed. Additionally, this paper delves into the economic significance of the date industry and its global trade.

Nutritional Traits, Pasting Properties and Antioxidant Profile of Selected Genotypes of Sorghum, Oat and Maize Eligible for Gluten-Free Products.

The technological and nutritional traits of food-grade sorghum hybrids, hulled/naked oat varieties and maize genotypes of different colors were studied for novel and healthier gluten-free foods. Oat genotypes showed the highest protein content, followed by maize and sorghum. The total starch and the total dietary fiber content were quite similar among the three species. Great variation was found in the amylose content, and the highest was in sorghum (27.12%), followed by oat 16.71% and maize 10.59%. Regarding the pasting profile, the rank of Peak Viscosity was sorghum (742.8 Brabender Unit, BU), followed by maize (729.3 BU) and oat (685.9 BU). Oat and sorghum genotypes had similar average breakdown (407.7 and 419.9 BU, respectively) and setback (690.7 and 682.1 BU, respectively), whereas maize showed lower values for both parameters (384.1 BU and 616.2 BU, respectively). The total antioxidant capacity, only in maize, significantly correlated with total flavonoid, phenolic and proanthocyanidin contents, indicating that all the measured compounds contributed to antioxidant capacity. The study indicated the importance of sounding out the nutritional and technological characteristics of gluten-free cereals in order to select suitable cultivars to be processed in different gluten-free foods with better and healthier quality.

Fermentation of millet bran with Bacillus natto: enhancement of bioactivity levels and the bioactivity of bran extract.

Millet bran (MB), a byproduct of millet production, is rich in functional components but it is underutilized. In recent years, researchers have shown that fermentation can improve the biological activity of cereals and their byproducts. This study used Bacillus natto to ferment millet bran to improve its added value and broaden the application of MB. The bioactive component content, physicochemical properties, and functional activity of millet bran extract (MBE) from fermented millet bran were determined. After fermentation, the soluble dietary fiber (SDF) content increased by 92.0%, the β-glucan content by 164.4%, the polypeptide content by 111.4%, the polyphenol content by 32.5%, the flavone content by 16.4%, and the total amino acid content by 95.4%. Scanning electron microscopy revealed that the microscopic morphology of MBE changed from complete and dense blocks to loosely porous shapes after fermentation. After fermentation, the solubility, water-holding capacity, and viscosity significantly increased and the particle size decreased. Moreover, the glucose adsorption capacity (2.1 mmol g-1), glucose dialysis retardation index (75.3%), and α-glucosidase inhibitory (71.4%, mixed reversible inhibition) activity of the fermented MBE (FMBE) were greater than those of the unfermented MBE (0.99 mmol g-1, 32.1%, and 35.1%, respectively). The FMBE presented better cholesterol and sodium cholate (SC) adsorption properties and the adsorption was considered inhomogeneous surface adsorption. Fermentation increased the bioactive component content and improved the physicochemical properties of MBE, thereby improving its hypoglycemic and hypolipidemic properties. This study not only resolves the problem of millet bran waste but also encourages the development of higher value-added application methods for millet bran. © 2024 Society of Chemical Industry.

Non-Traditional Vegetable Raw Materials Use in the Yeast-Free Biscuits Technology

Biscuits fall into the dry biscuit category, have a long shelf life and are technologically suitable for enrichment with non-traditional vegetable raw materials. The study aim is to improve the production technology of yeast-free biscuits using non-traditional vegetable raw materials – vegetable powders obtained from pumpkin and carrots. The researchers modernized classic recipe of biscuits “Arctica” by (I) the partial replacement of premium wheat flour with vegetable powders (pumpkin and carrot) at a concentration of 5 % and barley flour at a concentration of 30 % of the total weight of premium wheat flour and (II) butter replacement with a structured fat base (sunflower oil and hydrated potato starch). The thesis demonstrates the organoleptic, physico-chemical characteristics, nutritional and energy value of the developed yeast-free biscuits compared to the control sample. A man determined that the butter replacement with a structured emulsion of sunflower oil and hydrated starch enable to eliminate the saturated fat content in the biscuit formulation. The developed yeast-free biscuits have satisfactory consumer characteristics, a richer chemical composition with the same caloric content as the control sample, mainly due to the content of dietary fibers, minerals and vitamins contained in non-traditional vegetable raw materials.

Flavor and TASTE attributes and nutritional insights of maize tortillas from landraces of Mexican races

Maize tortilla is the best-recognized food product of Mexican gastronomy. Artisanal maize tortillas (AMT) are prepared with native maize varieties and a traditional process. The aims of this study were to identify sensory attributes, texture, and color in AMT that allow them to be differentiated from commercial tortillas, and to determine the chemical and mineral composition of both types of tortillas. Six landraces related to four Mexican maize races were used. Two commercial tortillas were included as references (tortillería and supermarket). Tortillas were subjected to sensory analysis by the modified Flash technique, texture and color were measured objectively and chemical and mineral analysis of all tortillas were evaluated. Lime taste and lime smell attributes were relevant to differentiate AMT from commercial tortillas; aftertaste and fracturability attributes were highly associated to supermarket tortillas. The fracturability attribute of tortillas is consider undesirable for taco preparation. Five of the six AMT were characterized by the presence of a layer, a characteristic that is associated with traditional tortilla made by Mexican consumer. Regarding chemical composition, supermarket tortillas exhibited the highest dietary fiber content (17.09%), but showed 30% more Na than AMT, with the exception of tortillas from Purepecha native variety. Besides, supermarket tortilla had 48.9% less Ca than AMT. The sensory attributes relevant to differentiate native maize tortillas from the commercial maize tortilla references were appearance, smell, and taste, while textural and color attributes played a lesser role.

Technological properties, shelf life and consumers' acceptance of high-fibre cookies prepared with juice processing by-products.

High dietary fibre cookies were manufactured from non-wheat (triticale, spelt and rye) flours with apple, beetroot and pumpkin pomace powders added as natural colourants. Cookies were characterized by nutritional composition, colour, texture and sensory profile and subjected to a 2-month shelf-life study. Additionally, an acceptability study was done to determine consumers' acceptance of cookies. Cookies prepared from rye flour and pumpkin pomace, as well as cookies prepared from spelt flour and beetroot pomace, can be labelled as 'high fibre' (dietary fibre content of 8.90% and 7.09%, respectively), while cookies prepared from triticale flour and apple pomace can be labelled as a 'source of fibre' (dietary fibre content of 4.50%). No obvious trend in the colour of cookies was observed after storage at room temperature and 40 °C, indicating the stability of natural colourants. Hardness decrease was observed in all samples after storage; however, the acceptability study showed that consumers prefer softer cookies. Sensory analysis showed that there were no signs of rancidity in samples after storage. Although triticale flour and apple pomace sample received the highest liking scores for appearance, odour and taste, all samples had liking ratings higher than 4 (indifferent) and can be further modified to satisfy consumers' demands.

Production of resistant starch type 3 from cassava starch with high shear mixing and centrifugation treatment to increase its total dietary fibre content

SummaryCassava starch has a big potential to be a source material for producing low‐calorie starch by converting it to resistant starch. However, its original structure can prevent retrogradation of the amylose fraction to produce resistant starch so that reduce the total dietary fibre value. In this research, high shear mixing (HSM) is used to assist the gelatinisation process to provide disintegration of the cassava starch structure. Centrifugation was carried out to separate the top layers which have a high content of free amylose resulting in better retrogradation. The starch–water suspension was processed using HSM at a rotational speed of 9000–12 000 at 95 °C for 15 min. Then, the starch suspension was separated by centrifugation for 30 min. The product was then analysed using total dietary fibre (TDF), component carbohydrate analysis, scanning electron microscope (SEM) and x‐ray diffraction (XRD). From the analysis results, this method can increase the TDF content of the product up to 14.66% at 12 000 rpm.

Enzymatic impregnation by high hydrostatic pressure for preparing shape-retaining softened broccoli and carrot

This study examined the feasibility of utilizing high-pressure processing (HPP) with enzymatic impregnation to soften vegetables. Freeze–thaw (FT) processing was used as a positive control to compare the hardness, physicochemical characteristics, and cell microstructure of vegetables. Carrot and broccoli samples were subjected to 200 MPa pressure for 15 min and then impregnated using a Viscozyme L enzyme solution. Compared with the hardness of fresh samples, the hardness of the carrot and broccoli samples that underwent HPP and enzymatic impregnation decreased by 84.6% and 95.5%, respectively, and the hardness of the carrot and broccoli samples that underwent FT processed decreased by 73.6% and 91.3%, respectively. In particular, HPP with enzymatic impregnation reduced the content of insoluble dietary fibers (18%), increased the content of soluble dietary fibers (3%), and considerably increased the concentration of carotene (1.9 times) and isothiocyanate (4.5 times) in carrots and broccoli, respectively. Scanning electron microscopy reveals that HPP physically damaged the intercellular compartments of the vegetables, thereby increasing the impregnation of enzymes. In addition, the level of HPP and the holding time increased, and the amount of vegetable residue and the degree of moisture retention substantially decreased, indicating a negative correlation between damage and hardness. Moreover, HPP pretreatment require a short processing time then FT, which promoted the penetration of enzymes and the internal microstructural biodegradation of vegetables, and it also helped these vegetables release more soluble nutrients. Overall, HPP can be used with enzymatic impregnation to obtain texture-modified vegetables for older consumers with mastication difficulties.

Effect of Fungal and Fungal-Bacterial Tempe-Type Fermentation on the Bioactive Potential of Grass Pea Seeds and Flaxseed Oil Cake Mix.

Tempe is an Indonesian food product traditionally obtained from soybeans through solid-state fermentation with Rhizopus. A variety of substrates can be processed into tempe in the presence of other microorganisms. In this study, grass pea seeds with the addition of flaxseed oil cake (20% w/w) were either fermented using individual mould strains-Rhizopus oryzae, R. oligosporus, and Mucor indicus-or cofermented with the moulds and Lactiplantibacillus plantarum. In the obtained products, the content of dietary fibre, B group vitamins, and the level of peptides and antioxidant potential in aqueous extracts were measured. Moreover, peptides, angiotensin I convertase inhibitor, and antioxidant activity were determined after in vitro digestion. The effect of digestates on the differentiation of enterocytes was also investigated. Fermentation generally resulted in a decrease in the dietary fibre, especially the insoluble fraction (30-50%). The product obtained with R. oryzae was the best source of riboflavin and thiamine among all tested. The fermentation process promoted the accumulation of water-soluble peptides and antioxidant compounds. After in vitro digestion, the largest amount of antioxidant and antiradical compounds was released from tempe obtained with R. oryzae. However, the enrichment of the products with antioxidants resulting from solid-state fermentation did not simply translate into an improvement in antioxidant potential after digestion. Generally, fermentation carried out in the presence of L. plantarum brought positive effects only in the case of R. oligosporus DSM 1964. Digestion products obtained from R. oryzae tempe had a positive effect on the viability of Caco-2 cells differentiated into enterocytes. Interestingly, a higher activity of differentiation markers (alkaline phosphatase and sucrase-isomaltase) was observed under the influence of digestate of R. oryzae and L. plantarum tempe.

Color and Nutritional Analysis of Ten Different Purple Sweet Potato Varieties Cultivated in China via Principal Component Analysis and Cluster Analysis.

Purple sweet potato (PSP) has abundant nutritional compounds, which are valuable constituents of the human diet, but its development and utilization are still in the primary processing phase. This study examined the differences in nutritional characteristics of 10 PSP varieties. A variety of nutritional components were evaluated and comprehensively compared using principal component analysis (PCA) and cluster analysis (CA). The PSP had 60.9-70.1% moisture. The dried PSP had abundant starch (43.9~67.2%) and dietary fiber (9.40~16.5%), moderate levels of protein (3.19~8.75%) and reducing sugar (1.44~4.01%), and low amounts of crude fat (0.51~1.01%). The anthocyanin profile varied significantly between the different varieties. A correlation analysis showed that a higher content of anthocyanins resulted in a darker color. The PCA and CA suggested that varieties XS, ZL, and JS18 are desirable for developing the diabetic patient's diet. JS1 had the highest anthocyanin, protein, and dietary fiber contents and the lowest starch, implying that it could be used as a source of natural colorants or functional foods. Varieties FX, GS, ES13, and EN are suitable for producing various starch-based food products, such as noodles, cookies, and pastries. This study provides a reference for the practical use and rational processing of PSP resources.