DE Content Research Articles

Rapid determination of the content of digestible energy and metabolizable energy in sorghum fed to growing pigs by near-infrared reflectance spectroscopy1.

The object of this study was to establish a new method to predict the content of DE and ME in sorghum fed to growing pigs by using near-infrared reflectance spectroscopy (NIRS). A total of 33 sorghum samples from all over China were used in this study. The samples were scanned for their spectra in the range of 12,000 to 4,000 cm-1. Based on principal components analysis of the spectra, the samples were split into a calibration set (n = 24) and a validation set (n = 9) according to the ratio of 3:1. With animal experiment values as calibration reference, the calibration models of DE and ME were established using partial least squares regression algorithm. Different spectral pretreatments were applied on the spectra to reduce the noise level. The best wavenumber ranges were also investigated. Results showed that DE and ME content in sorghum fed to growing pigs ranged from 14.57 to 16.70 MJ/kg DM and 14.31 to 16.35 MJ/kg DM, respectively. The optimal spectral preprocessing method for DE and ME was the combination of first derivative and multiplicative scatter correction. The most informative near-infrared spectral regions were 9,403.9 to 6,094.4 cm-1 and 4,605.5 to 4,242.9 cm-1 for both DE and ME. The best performance for DE and ME calibration models was the coefficient of determination of calibration (R2c) of 0.94 and 0.93, coefficient of determination of cross-external validation (R2cv) of 0.88 and 0.86, residual predictive deviation of cross-external validation (RPDcv) of 2.86 and 2.64, coefficient of determination of external validation (R2v) of 0.90 and 0.81, and residual predictive deviation of external validation (RPDv) of 3.15 and 2.35, respectively. There were no significant differences between the measured and NIRS predicted values for DE and ME (P = 0.895 for DE and P = 0.644 for ME). As the number of calibration samples increased from 24 to 33, the calibration performance of DE and ME models was improved, indicated by increased R2c, R2cv, and RPDcv values. In conclusion, NIRS quantitative models of the available energy in sorghum were established in this study. The results demonstrated that the content of DE and ME in sorghum could be predicted with relatively high accuracy based on NIRS and NIRS showed the superiority of speediness and practicality when compared with previous research methods including animal experiments, regression equations, and computer-controlled simulated digestion system.

PENGARUH KADAR PROTEIN PAKAN YANG BERBEDA DENGAN RASIO E/P 8,5 kkal/g PROTEIN TERHADAP PERTUMBUHAN IKAN NILA (Oreochromis niloticus

Feed utilization by tilapia (Oreochromis niloticus) was less optimal. Feed utilization efficiency (FUE) by tilapia could be improved by feed them with the suitable E/P ratio value of feed. This studi was aimed to examine the effect of various dietary protein with the E/P ratio of 8,5 kcal/g protein on the growth of nile tilapia. The experimental fish used was nile tilapia with the average body weight of 0,50±0,02 g/fish. The feed was given three times a day i.e. at 8.00 am, 12.00, and 4.00, pm by applied at satiation method. The experimental fish was maintained for 35 days with the density of 1 fish/l. The experiment used was completely randomized design with 4 treatments and 3 replicates. The treatments A, B, C, and D were trial feeds with protein content of 28, 30, 33, and 36% with DE content 240, 255, 280.5, and 306 kcal, respectively. The variables measured were total feed consumption (TFC), ratio efficiency protein (REP), specific growth rate (SGR), and survival rate (SR). The data showed that various dietary protein with equal E/P ratio resulted significantly effect (P<0,05) on FUE, REP, and SGR; but did not significantly effect (P>0,05) on TFC and SR. The TFC values of A, B, C, and D were 17,84±1,24, 18,70±0,28, 17,42±0,53, and 18,65±0,34 g; FUE values were 27,20±2,49, 38,19±3,84, 41,05±1,74, and 44,49±5,60%; REP values were 0,96±0,09, 1,28±0,13, 1,26±0,05, and 1,25±0,16%; and SGR values were 1,46±0,08, 1,94±0,28, 1,95±0,08, and 2,05±0,30%/day. The SR values of A, B, C and D were 100±0,00, 97,78±3,85, 97,78±3,85, and 95,33±4,04%, respectively. It was suggested that the increase of dietary energy and protein with a certain level of E/P ratio could improve the feed utilization efficiency and growth of tilapia

In vitro unfermented fiber is a good predictor of the digestible and metabolizable energy content of corn distillers dried grains with solubles in growing pigs1

Characterizing fiber into fermentable and unfermentable fractions may enhance the accuracy of estimating DE and ME energy content in fiber-rich ingredients. Therefore, the objective of this study was to analyze the concentrations of NDF, representing both the fermentable (fNDFom) and unfermentable (uNDFom) portions among sources of corn distillers dried grains with solubles (DDGS), and determine their relative contributions to DE and ME content. The concentrations of DE and ME, as well as apparent total tract digestibility (ATTD) of GE, were measured in a previous experiment. Samples of DDGS (0.5 g) were mixed with fecal inoculum and incubated for 8, 12, and 72 h. The ash corrected NDF (NDFom) content of DDGS residues at each time point was determined. The fNDFom increased with fermentation time of 8 h (21.6%), 12 h (29.0%), and 72 h (68.6%). The ATTD of GE increased as the uNDFom decreased at 8 h (uNDFom8; R2 = 0.83; P < 0.01) and 72 h (uNDFom72; R2 = 0.83; P < 0.01). Likewise, ME content of DDGS increased as uNDFom72 decreased (R2 = 0.59; P < 0.01). The best-fit DE equation was DE (kcal/kg DM) = 2,175 - 3.07 × uNDFom8 (g/kg, DM) - 1.50 × uNDFom72 (g/kg, DM) + 0.55 × GE (kcal/kg DM) (R2 = 0.94, SE = 36.21). The best-fit ME equation was ME (kcal/kg DM) = 1,643 - 2.31 × uNDFom8 (g/kg, DM) - 2.54 × uNDFom72 (g/kg, DM) + 0.65 × GE (kcal/kg DM) - 1.42 × crude protein (g/kg DM) (R2 = 0.94, SE = 39.21). These results indicate that in vitro unfermented fiber is negatively associated with GE and NDF digestibility, and therefore, is a good predictor of DE and ME content in corn-DDGS.

Energy values of solvent-extracted canola meal and expeller-derived canola meal for broiler chickens and growing pigs determined using the regression method1.

The energy values of solvent-extracted canola meal (SECM) and expeller-derived canola meal (EDCM) for broiler chickens and growing pigs were determined in 2 experiments using the regression method. Corn-soybean meal reference diet (RF) and 4 test diets were prepared. The test diets consisted of SECM or EDCM that partly replaced the energy sources in the RF at 100 or 200 g/kg, respectively. The ratios of all energy ingredients were kept similar across all experimental diets. In Exp. 1, a total of 300 birds were fed standard broiler starter diet from days 0 to 19 posthatching. On day 19, 240 birds (776 ± 79.3 g initial BW) were assigned into 5 experimental diets in a randomized complex block design with BW as a blocking factor. Excreta were collected from days 23 to 25 and ileal digesta were collected after birds were euthanized by CO2 asphyxiation on day 26. In Exp. 2, 40 barrows (28.4 ± 1.6 kg initial BW) were allotted to 5 experimental diets according to the randomized complete block design with BW as a blocking factor. After 5-d adaption period, the feces and urine samples were collected for 5 d by total collection method. The ileal digestible energy (IDE), apparent ME (AME), and nitrogen-corrected apparent ME (AMEn) in Exp. 1 and the DE, AME, and AMEn in Exp. 2 for experimental diets and canola meals were determined. In Exp. 1, the inclusion of canola meals to RF linearly decreased the IDE, AME, and AMEn for birds fed SECM diets (P < 0.01) and the AME and AMEn for birds fed EDCM diets (P < 0.01). Furthermore, quadratic effects were also found in the IDE, AME, and AMEn by the inclusion of EDCM to RF (P < 0.05). The IDE were 2,194 and 3,514 kcal/kg DM for SECM and EDCM in broiler chickens, respectively. The respective ME and MEn values were 1,919 and 1,695 kcal/kg DM for SECM and 3,134 and 2,937 kcal/kg DM for EDCM. In Exp. 2, the SECM or EDCM addition to RF linearly decreased the AME and AMEn for pigs (P < 0.01). The DE content was also decreased linearly with the increasing level of SECM (P < 0.01). The DE, ME, and MEn of SECM for pigs were 3,109, 2,891, and 2,655 kcal/kg DM, respectively. The EDCM contained 3,850 kcal of DE, 3,581 kcal of ME, and 3,491 kcal of MEn/kg DM for pigs. In conclusion, the energy values of EDCM are greater than those of SECM for broiler chickens and pigs, and pigs utilize more of the GE in SECM and EDCM than broiler chickens.

Digestibility of energy and caloric value in nursery pigs fed commercially available lipids

ABSTRACT Objective The objective was to determine the DE of various lipids and compare these values with predicted values based on published equations. Materials and Methods Pigs (480) weaned at 24 d of age were fed a common diet for 7 d after which they were fed a basal or lipid-based diet for 28 d. Lipids (7.13%) were added in place of the basal diet, and all diets were pelleted and offered ad libitum. Fecal samples were collected on d 25 from 2 pigs per pen and pooled for subsequent analysis. Apparent total-tract digestibility of GE of the diet was determined by the indirect method and the DE content of each lipid source using the difference procedure. Results and Discussion The DE for butter fat, canola oil, coconut oil, fish oil, flaxseed oil, lard, olive oil, palm oil, soybean oil, and tallow was 8,911, 9,474, 9,380, 9,464, 8,584, 8,648, 9,606, 8,304, 9,979, and 8,071 kcal/kg, respectively. Using these values and the chemical composition of each lipid, the best fit prediction equation was DE, kcal/kg = 10,267 − (110.3 × FFA, %) − (41.8 × C16:0, %) − (39.7 × C18:0, %) − (98.0 × unsaturated:saturated ratio) + (6.4 × iodine value), SE = 164, R2 = 0.97, P = 0.01. Implications and Applications The determined DE herein were approximately 12% greater than predicted based on published equations, indicating the refining of published prediction equations is needed. These results indicate that DE of lipids can vary depending on their fatty acid composition, which consequently affects pig performance.

Effect of flaxseed oil, animal fat, and vitamin E supplementation on growth performance, serum metabolites, and carcass characteristics of finisher pigs, and physical characteristics of pork

Dietary lipid supplementation can reduce de novo lipogenesis and increase intramuscular fat content of pork simultaneously, and vitamin E supplementation can increase the oxidative stability of pork. A total of 96 pigs (Yorkshire) were used to investigate the effect of dietary lipid (0 or 1% flaxseed oil + 1, 3, or 5% poultry fat for diets supplemented with lipids) and vitamin E (11 or 220 IU/kg) supplementation in a 4 × 2 factorial arrangement of treatments on growth performance, serum metabolites, and carcass traits of finisher pigs, and physical characteristics of pork. Forty-eight pens containing 2 gilts or 2 castrated males per pen were randomly assigned to 8 diets with 3 gilt pens and 3 castrated male pens per treatment. Pigs were switched from finisher-1 [initial body weight (BW): 54.3 ± 3.4 kg] to finisher-2 diets when the average pen BW reached 84.1 ± 4.0 kg. Standardized ileal digestible Lys and Ca and P were adjusted with the DE content accordingly to maintain constant Lys, Ca, and P to DE ratios. Pigs were harvested when they reached the target BW of 110.0 ± 3.0 kg. There were no lipid x vitamin E interactions in growth performance. As dietary lipids increased from 0 to 6%, average daily feed intake decreased linearly (P < 0.018) and gain to feed ratio improved linearly (P < 0.001) during the finisher-1, finisher-2, and overall phases. Supplementation with 220 IU vitamin E/kg reduced the efficiency of feed, Lys, and DE utilization for BW gain (P < 0.036) during the finisher-1 and overall phases. As dietary lipids increased, serum cholesterol and triglyceride concentrations increased linearly (P < 0.003). Supplementation of the diet with 1% flaxseed oil + 1, 3, or 5% poultry fat resulted in greater serum total protein, albumin, cholesterol, and triglyceride concentrations (P < 0.037) compared with no lipid supplementation. Pigs fed the diets containing 0, 2, and 4% lipids and supplemented with 220 IU vitamin E/kg had greater 10th rib BF and lower fat-free lean than those fed the dies contained 11 IU vitamin E/kg, whereas 10th rib BF was lower and fat-free lean was greater in pigs those fed the diet containing 6% lipids and supplemented with 200 IU vitamin E/kg than those fed the diet contained 11 IU vitamin E/kg (lipid x vitamin E, P < 0.020). Dietary lipids had no effect on subjective marbling score and ether extract content of loin muscle or most other pork and loin muscle characteristics, but belly thickness and width increased (linear, P < 0.040) as dietary lipids increased. Vitamin E supplementation increased marbling score (P = 0.042), but it had no clear effect on loin muscle color (subjective, and L*, a*, and b*), ham and loin muscle pH, loin muscle characteristics, or belly firmness. As expected, feed intake decreased and feed efficiency improved progressively with increasing dietary lipid supplementation from 0 to 6%. Although there were interactions, dietary lipids seemed to have no clear effects on the carcass BF or fat-free lean. Similarly, dietary supplementation with 1% flaxseed oil + 1, 3, or 5% poultry fat had no effect on subjective marbling score or ether extract content of loin muscle. Supplementation with 220 IU vitamin E/kg increased carcass leanness with 6% dietary lipids, but increased carcass BF with 0 to 4% dietary lipids. Vitamin E supplementation increased subjective marbling score, but seemed to have no clear effects on loin muscle color, ham and loin muscle pH, loin muscle characteristics, or pork belly firmness.

Determination of chemical composition, energy content, and amino acid digestibility in different wheat cultivars fed to growing pigs.

This study was conducted to determine the DE, ME, and apparent (AID) and standardized ileal digestibility (SID) of AA in different wheat cultivars fed to growing pigs. In Exp. 1, twelve crossbred growing barrows were allotted to a replicated 6 × 6 Latin square design to determine the DE and ME contents of 12 different wheat cultivars. In Exp. 2, twelve growing barrows with a simple T-cannula were used to determine the AID and SID of AA in 10 different wheat cultivars. Pigs were randomly allotted to a replicated 6 × 6 Latin square design and fed one nitrogen-free diet and 10 different cultivars of wheat diets. Among different wheat cultivars, the concentrations of GE, CP, NDF, ADF, and starch ranged from 4,385 to 4,458 kcal/kg, 12.95% to 18.14%, 9.16% to 13.89%, 1.93% to 2.92% and 60.81% to 70.77%, respectively (DM basis). There were significant differences (P < 0.05) in the DE, ME values, and the ATTD of GE in 12 different cultivars of wheat in Exp. 1. The DE and ME contents and the ATTD of GE ranged (P < 0.05) from 3,922 to 4,067 kcal/kg DM, 3,759 to 3,941 kcal/kg DM and 88.14% to 90.31%, respectively. The best prediction equations of DE and ME for different cultivars of wheat cultivars were DE = -2,738 - (40.8 × % ADF) + (1.7 × GE) - (51.5 × % Xylans) - (95.7 × % Ash) + (22.3 × % EE), R2 = 0.98, and ME = -2,990 + (1.7 × GE) - (50.2 × % Xylans) - (87.6 × % Ash), R2 = 0.88, respectively. There were also differences (P < 0.05) in the AID and SID of CP and indispensable AA, expect for His, in 10 wheat cultivars in Exp. 2. The AID and SID of CP, Lys, and Met ranged (P < 0.05) from 82.02% to 89.46%, 74.13% to 84.73%, 87.35% to 92.49% and 87.56% to 94.04%, 80.56% to 89.89%, 89.56% to 94.45%, respectively. In conclusion, the chemical composition, energy contents, and most AA digestibility in different cultivars of wheat varied widely.

Nutritive value of cowpea fodder cultivars and their effect on nutrients intake, digestibility and nitrogen balance in sheep

Three cowpea fodder varieties (BL-1, BL-2 and BL-4) developed by IGFRI were evaluated against check Kohinoor for their nutritional attributes by in vitro and in vivo means. Varieties were grown under identical agronomic practices in Kharif 2016 and animal feeding was started at flower initiation stage. For feeding trial, 20 growing sheep (mean body weight 19.85±0.45 kg) were randomly distributed to 4 varieties (Kohinoor, BL-1, BL-2 and BL- 4) with 5 animals to each. After 3 weeks of feeding, a digestion cum- metabolism trial was conducted to assess the nutrients intake, digestibility and nitrogen balance in sheep. The cowpea fodder varieties CP, NDF and lignin contents differed and values ranged between 22.23–23.41, 50.54–55.10 and 8.48–10.76%, respectively. Protein and carbohydrate fractions of cowpea varieties were similar except the NSC (% DM) and CC (% CHO) which were lower and higher, respectively in BL-2. Cowpea varieties energy values as TDN, DE, NEL, NEM and NEG were identical. In vitro gas and methane production (ml/g DM) was lower from Kohinoor (86.57 and 10.67) and BL-2 (90.79 and 11.40) than BL-1 (104.83 and 13.96) and BL-4 (98.86 and 13.08), respectively. Short chain fatty acids, partition factor and efficiency for microbial production differed significantly amongst the cowpea varieties. Sheep DM, CP and ME intake (% body wt and g/kg W0.75) was comparable on evaluated cowpea varieties. Digestibility of DM, OM and CP in sheep was similar on cowpea varieties; however, NDF and ADF digestibility tended to be lower of BL-4 (63.88 and 61.70%) than BL-2. Sheep nitrogen balance was identical on all the cowpea varieties and ranged between 16.92–18.32 g/d. Cowpea varieties DCP and DE contents were similar and ranged between 18.36– 20.30% and 13.63–14.72 kjoules/g DM, respectively. Results showed that nutritive value of cowpea varieties differed significantly in terms of CP, NDF, NSC%DM, fermentation pattern, and cell wall (NDF and ADF) digestibility.

Determination of net energy content of dietary lipids fed to growing pigs using indirect calorimetry.

The objective of this experiment was to determine the NE content of different dietary lipids fed to growing pigs using indirect calorimetry. Thirty-six growing (initial BW: 41.1 ± 3.1 kg) barrows were allotted to 6 diets based on completely randomized design with 6 replicate pigs per diet. Diets included a corn-soybean meal basal diet and 5 test diets each containing 10% palm oil, poultry fat, fish oil, corn oil, or flaxseed oil at the expense of corn and soybean meal. During each period, pigs were individually housed in metabolism crates for 14 d, which included 7 d for adaptation to feed, metabolism crates, and environmental conditions. On day 8, pigs were transferred to the open-circuit respiration chambers and fed 1 of the 6 diets at 2.3 MJ ME/kg BW0.6/day. Total feces and urine were collected and daily heat production (HP) was also calculated from day 9 to day 13. On the last day of each period (day 14), pigs were fasted and the fasting heat production (FHP) was measured. The results show that the FHP of pigs averaged 809 kJ/kg BW0.6·day-1 and was not affected by diet characteristics. The DE values were 35.98, 36.84, 37.11, 38.95, and 38.38 MJ/kg DM, the ME values were 35.79, 36.56, 36.92, 37.73, and 38.11 MJ/kg DM, and the NE values were 32.42, 33.21, 33.77, 34.00, and 34.12 MJ/kg DM, for the palm oil, poultry fat, fish oil, corn oil, and flaxseed oil, respectively. Based on our result, we concluded that the DE content of dietary lipid varied from 91% to 98% of its GE content, the ME content of dietary lipid was approximately 99% of its DE content, and the NE content of dietary lipid was approximately 90% of its ME content in growing pigs.

362 Quantification of Basal Endogenous Fat Losses and Their Impact on Estimates of the De Content of Dietary Fat Fed to 13 Kg and 50 Kg Pigs`.

362 Quantification of Basal Endogenous Fat Losses and Their Impact on Estimates of the De Content of Dietary Fat Fed to 13 Kg and 50 Kg Pigs`.

Prediction of digestible and metabolizable energy of corn distillers dried grains with solubles for growing pigs using in vitro digestible nutrients.

The objective of this study was to develop prediction equations (EQS) that estimate DE and ME content of corn distillers dried grains with solubles (DDGS) using digestible (DIG) nutrients from in vitro or in vivo assays. Chemical composition data from 12 sources of corn DDGS were obtained using laboratory chemical analysis (LCA) and near infrared spectroscopy (NIRS). In vitro DM disappearance (IVDMD) from gastric and small intestine hydrolysis (IVDMDh), large intestine fermentation (IVDMDf), and total tract digestion (IVDMDt) were also determined along with in vivo apparent total tract digestibility (ATTD) of DM, CP, ether extract (EE), NDF, and ADF, and energy values (GE, DE, and ME). Correlation analysis was used to compare chemical composition from LCA with NIRS, and a stepwise selection of variables was performed using linear regression to establish DE and ME prediction EQS. Composition determined by NIRS did not correlate with values from LCA. Consequently, significance and R2 were poorer when using NIRS data as inputs to predict DE (P = 0.11; R2 = 0.23) and ME (P = 0.11; R2 = 0.24). However, when using LCA data, DE (P = 0.04; R2 = 0.35) and ME (P = 0.04; R2 = 0.52) estimates of corn DDGS obtained from prediction EQS had significant P values, but low R2. Better prediction of DE (P < 0.01; R2 = 0.83) and ME (P < 0.01; R2 = 0.76) was observed when using in vivo DIG nutrients as inputs (DM basis) for DE, kcal/kg = 854.5 + (3.6 × DIG DM, g/kg) + (3.7 × DIG EE, g/kg) + (2.0 × DIG NDF, g/kg) and ME, kcal/kg = 704.5 + (3.3 × DIG DM, g/kg) + (4.8 × DIG EE, g/kg) + (2.6 × DIG NDF, g/kg). In vitro DIG DM, but not in vitro DIG NDF, was selected for use in EQS (7) DE, kcal/kg = 6,383.6 - (42.5 × in vitro DIG DM, g/kg) + (35.4 × DIG EE, g/kg) and (8) ME, kcal/kg = 6,635.1 - (49.8 × in vitro DIG DM, g/kg) + (41.3 × DIG EE, g/kg), but the significance and accuracy for both DE (P = 0.07; R2 = 0.45) and ME (P = 0.05; R2 = 0.49) predictions was less using in vitro DIG nutrient values than using in vivo DIG nutrient values. If chemical composition was used to replace in vivo EE, along with in vitro DIG NDF and DM, the prediction EQS only used the concentration of ADF and EE as predictors instead of in vitro DIG NDF and DM. In conclusion, in vivo DIG NDF, DM, and EE are the best predictors for DE and ME content of corn DDGS for swine. Using NIRS to determine chemical composition, in vitro DIG NDF, and in vitro DIG DM did not result in accurate predictions of DE and ME.

Chemical composition, energy, and amino acid digestibility in 7 cottonseed co-products fed to growing pigs.

The objective of this study was to determine the chemical composition, DE and ME contents, and apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA in 7 cottonseed co-products fed to growing pigs. The 7 cottonseed co-products were: cottonseed meals (solvent extracted) with CP level of 46%, 50%, and 55% (46CSM, 50CSM, and 55CSM), cottonseed protein with CP level of 50% and 55% (50CSP and 55CSP), fermented cottonseed meal (CSMF), and expelled cottonseed meal (CSME). The DE and ME contents of the 7 test ingredients were measured using 48 crossbreed barrows (BW: 44.1 ± 4.2 kg) and 8 diets (including a corn-soybean meal basal diet) in a completely randomized design. The AID and SID of AA of the 7 test ingredients were determined using 7 crossbreed barrows (initial BW: 67.4 ± 8.5 kg) in a 6 × 8 Youden square design with 6 periods and 8 diets (including one N-free diet). The DE and ME values of the 7 cottonseed co-products varied from 12.72 to 15.63 MJ/kg and 12.24 to 14.83 MJ/kg, respectively. Among the 7 cottonseed co-products, 55CSP and CSME had the greatest (P < 0.05) ME and DE values compared to the other cottonseed co-products. In addition, 55CSP had the greatest (P < 0.05) AID and SID of all the AA tested except for Gly and Pro. In contrast, 46CSM or 55CSM had the lowest (P < 0.05) AID and SID of all the AA tested except for Gly and Pro. The 55CSP also had the greatest (P < 0.05) concentrations of standardized ileal digestible CP and all the AA tested except for Gly and Pro. In conclusion, the chemical composition, energy, and AA digestibility of cottonseed co-products with different processing techniques varied widely. Based on the energy and AA digestibility, cottonseed protein with CP level of 55% is a better dietary ingredient for growing pigs compared with the other cottonseed co-products.

Concentration of digestible and metabolizable energy, standardized ileal digestibility, and growth performance of pigs fed diets containing sorghum produced in the United States or corn produced in China.

The DE and ME content (Exp. 1) as well as the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of essential AA (EAA; Exp. 2) were compared between Chinese corn and U.S. sorghum. The effects of U.S. sorghum as a potential substitute for Chinese corn on growth performance of 114 weaned pigs (8.8 ± 1.0 kg BW; Exp. 3) and 60 growing pigs (23.4 ± 1.6 kg BW; Exp. 4) were evaluated, and the effect of protease supplementation on N utilization was determined in sorghum-based diets fed to growing pigs (Exp. 4). In Exp. 1, there was no difference in DE and ME content between corn and sorghum. In Exp. 2, the AID and SID of most EAA and the concentrations of standardized ileal digestible Lys, Met, Thr, and His were less in sorghum than in corn ( < 0.05). In Exp. 3, there was no difference in ADG and ADFI among treatments during the experimental period. The G:F and apparent total tract digestibility (ATTD) of CP was decreased for pigs fed diets with sorghum in the first 2 wk ( < 0.05) and for pigs fed diets containing 60% sorghum in the following 2 wk ( < 0.05). The fecal score for pigs fed diets with sorghum, regardless of the substitute level, was less ( < 0.05) or tended to be less ( = 0.086) than that for pigs fed diets containing 60% corn. In Exp. 4, no differences were observed in ADG and ADFI overall among pigs fed diets based on corn and soybean meal (CSBM) or sorghum and soybean meal (SSBM). Pigs fed CSBM or SSBM with protease supplementation had greater ( < 0.05) or tended to have greater ( = 0.062) G:F than pigs fed SSBM. Compared with CSBM, SSBM increased fecal N excretion by more than 25% and decreased the ATTD of CP by more than 7% during the whole experiment ( < 0.05). Protease supplementation reduced fecal N excretion by more than 12% and increased ATTD of CP by more than 6% ( < 0.05). In conclusion, based on optimal G:F and CP digestibility, diets for weaned pigs should contain less than 20% sorghum during the first 2 wk and no more than 40% during the subsequent 2 wk after weaning. Sorghum used as an alternative energy source for corn in diets fed to growing pigs decreases CP utilization by increasing manure N output, which might be partially offset by protease supplementation.

Viscous and fermentable nonstarch polysaccharides affect intestinal nutrient and energy flow and hindgut fermentation in growing pigs.

Two experiments were conducted to determine the effects of viscosity and fermentability of purified nonstarch polysaccharides (NSP) on intestinal nutrient and energy flow and hindgut fermentation in growing pigs. In Exp. 1, 15 ileal-cannulated pigs (50.5 ± 2.9 kg BW) were allotted to 5 diets in a 2-period incompletely randomized design. Pigs were provided a cornstarch N-free diet (CST) or a diet containing 5% inulin (INU), carboxymethylcellulose sodium (CMC), microcrystalline cellulose (MCC), or Solka-Floc (SFC). Apparent ileal digestibility (AID) of DM, ash, and GE were greater ( < 0.05) in the MCC and SFC diets than in the INU and CMC diets. Apparent total tract digestibility (ATTD) of DM, carbohydrates (CHO), and GE and the DE content in the CST and INU diets were greater ( < 0.01) than in the CMC, MCC, and SFC diets. Hindgut disappearance of DM, CHO, and GE in the INU diet was greater ( < 0.05) than in the other N-free diets. The ileal endogenous flow of His, Ile, Leu, Met, Thr, Val, and all dispensable AA in the CMC diet was greater ( < 0.05) than in the other diets. In Exp. 2, 6 ileal-cannulated pigs were allotted to 3 diets containing either a corn-soybean meal control diet or the control diet with 5% INU or CMC in a replicated 3 × 3 Latin square design. Standardized ileal digestibility (SID) of Ala, Arg, Asp, Cys, Gly, His, Ile, Leu, Lys, Phe, Ser, Thr, and Val were greater ( < 0.05) in the CMC diet than in the control and INU diets. Incorporation of 5% INU or CMC in the control diet reduced ( < 0.01) the AID of arabinoxylan and insoluble and total NSP. The ATTD of NSP and cellulose and the hindgut disappearance of NSP, β-glucan, and cellulose in the CMC diet were less ( < 0.01) than in the control and INU diets. Inclusion of 5% INU in the diet increased ( < 0.01) hindgut fermentation of insoluble and total NSP compared with the control and CMC diets. In conclusion, depending on the viscosity and fermentability of the NSP, different sources will have different effects on nutrient digestibility and hindgut fermentation. Addition of 5% INU to a corn-soybean meal diet reduced digestibility of the NSP component in the ileum and increased hindgut fermentation of total NSP. In contrast, the inclusion of CMC increased the AID and SID of the diet and reduced total tract digestion and hindgut fermentation of NSP component. Carboxymethylcellulose sodium is not recommended as a source of synthetic fiber in a N-free diet to determine the SID of AA of diets.

A Bottom-Up Discursive Approach to Genetically Modified Organisms

The debate on genetically modified foods has been characterized by a heteroglosia of voices, ranging from experts, authorities, to scholars, community organizations, media, or consumers. Within such a context where there have been observed various food safety scares over the past decades or some public ignorance regarding the use of biotechnology related to food issues, the Debating Europe platform launched debates on arguments for and against GMOs. Thus citizens turn into prosumers of content regarding food safety and (de)legitimators of EU policies and/or other social institutions. In our study of the comments posted by e-citizens on four debates on GM foods launched on the Debating Europe platform, we will use framing theory (Goffman, 1974; Entman, 1993; Wynne, 2001; Cook et al., 2004) and the Integrated Crisis Mapping Model (Jin et al., 2012) for negative emotions and Plutchik’s wheel of emotions (2001) for positive emotions to determine the salience of (de)legitimating frames employed by the supporters and opponents of GM foods, and to examine the role of affective stance in the evaluation of the social actors related to GMOs. Using QDA miner and WordStat, computer assisted qualitative data analysis softwares, we will conduct a content, cluster and correspondence analysis of the (de)legitimating frames and emotion valences. Whereas the content analysis provides an insight into the frequency of frames used by e-debaters and into the key lexical items that express evaluation and affective stance related to GM foods, the explorative analyses reveal the types of (sub)clusters which prevail in the e-debaters’ comments on GM foods. The findings of this study suggest that e-citizens mainly perceive the issue of GMO through a political frame associated with anger and a health frame associated with fright.

Digestible, metabolizable, and net energy of camelina cake fed to growing pigs and additivity of energy in mixed diets1,2

This experiment was conducted to determine the DE, ME, and NE contents of camelina cake (CC) and to test the hypothesis that dietary glucosinolates originating from CC will affect the additivity of energy in mixed diets containing different inclusion levels of corn, soybean meal (SBM), and CC. A total of 30 growing barrows ([Yorkshire × Landrace] × Duroc) with a mean BW of 16.8 kg (SD 1.4) were randomly allotted to 1 of 5 treatments with 6 replicates per treatment. Pigs were fed experimental diets for 16 d, including 10 d for adaptation and 6 d for total collection of feces and urine. The 5 experimental diets consisted of 3 corn-based diets to determine the DE, ME, and NE of the 3 ingredients (corn, SBM, and CC) and 2 mixed diets to test the additivity of DE, ME, and NE. The corn diet contained 97.52% corn; the SBM diet contained 67.52% corn and 30.0% SBM; the CC diet contained 67.52% corn and 30.0% CC; the Mixed diet 1 contained 67.52% corn, 20.0% SBM, and 10.0% CC; and the Mixed diet 2 contained 67.25% corn, 10.0% SBM, and 20.0% CC. Vitamins and minerals were included in the diets to meet or exceed the requirements for growing pigs (NRC, 2012). Pigs were fed their assigned diets at 550 kcal ME/kg BW0.60 per day on the basis of BW on d 1, 5, and 10, which was close to ad libitum intake. Pigs had free access to water. Determined DE, ME, and NE contents of corn were 3,348, 3,254, and 2,579 kcal/kg, respectively; those of SBM were 3,626, 3,405, and 2,129 kcal/kg, respectively; and those of CC were 3,755, 3,465, and 2,383 kcal/kg, respectively. No differences between the predicted and determined DE, ME, and NE were observed in the 2 mixed diets. In conclusion, DE, ME, and calculated NE content of CC fed to growing pigs were 3,755, 3,465, and 2,383 kcal/kg (as-fed basis), respectively. In addition, additivity of DE, ME, and calculated NE was observed in the mixed diets containing corn, SBM, and CC, which indicates that dietary glucosinolates originating from up to 30% of CC inclusion do not affect DE, ME, and calculated NE of diets.

The digestible energy, metabolizable energy, and net energy content of dietary fat sources in thirteen- and fifty-kilogram pigs1

The objective was to determine the energy concentration of a diverse array of dietary fat sources and, from these data, develop regression equations that explain differences based on chemical composition. A total of 120 Genetiporc 6.0 × Genetiporc F25 (PIC, Inc., Hendersonville, TN) individually housed barrows were studied for 56 d. These barrows (initial BW of 9.9 ± 0.6 kg) were randomly allotted to 1 of 15 dietary treatments. Each experimental diet included 95% of a corn–soybean meal basal diet plus 5% either corn starch or 1 of 14 dietary fat sources. The 14 dietary fat sources (animal–vegetable blend, canola oil, choice white grease source A, choice white grease source B, coconut oil, corn oil source A, corn oil source B, fish oil, flaxseed oil, palm oil, poultry fat, soybean oil source A, soybean oil source B, and tallow) were selected to provide a diverse and robust range of unsaturated fatty acid:SFA ratios (U:S). Pigs were limit-fed experimental diets from d 0 to 10 and from d 46 to 56, providing a 7-d adaption for fecal collection on d 7 to 10 (13 kg BW) and d 53 to 56 (50 kg BW). At 13 kg BW, the average energy content of the 14 sources was 8.42 Mcal DE/kg, 8.26 Mcal ME/kg, and 7.27 Mcal NE/kg. At 50 kg BW, the average energy content was 8.45 Mcal DE/kg, 8.28 Mcal ME/kg, and 7.29 Mcal NE/kg. At 13 kg BW, the variation of dietary fat DE content was explained by DE (Mcal/kg) = 9.363 + [0.097 × (FFA, %)] − [0.016 × omega-6:omega-3 fatty acids ratio] − [1.240 × (arachidic acid, %)] − [5.054 × (insoluble impurities, %)] + [0.014 × (palmitic acid, %)] (P = 0.008, R2 = 0.82). At 50 kg BW, the variation of dietary fat DE content was explained by DE (Mcal/kg) = 8.357 + [0.189 × U:S] − [0.195 × (FFA, %)] − [6.768 × (behenic acid, %)] + [0.024 × (PUFA, %)] (P = 0.002, R2 = 0.81). In summary, the chemical composition of dietary fat explained a large degree of the variation observed in the energy content of dietary fat sources at both 13 and 50 kg BW.

Standardized ileal digestible amino acids and digestible energy contents in high-protein distiller's dried grains with solubles fed to growing pigs1

Chemical composition and, therefore, potential nutritive value of corn distiller's dried grains with solubles (DDGS) is constantly evolving as the ethanol industry innovates processing techniques. We determined standardized ileal digestibility (SID) of AA (Exp. 1) and DE (Exp. 2) contents in high-protein (HP; >40% on a DM basis) DDGS samples. Two HP DDGS (HP-A and HP-B) samples and 1 conventional DDGS (CON) sample were selected for this study. Three semipurified corn starch-based diets (minimum 18% CP, as-fed basis) were formulated with DDGS as the sole source of AA. A fourth, N-free diet was prepared to estimate basal ileal endogenous AA losses. The corn starch:sucrose:oil ratio was kept constant across diets to allow calculation of DE content in DDGS by the difference method. In Exp. 1, 8 ileal-cannulated barrows (23.9 kg initial BW) were used in a replicated 4 × 4 Latin square design ( = 8). Pigs were fed at 2.8 × maintenance energy requirement. In each period, pigs were adjusted to diets for 5 d followed by a 2 d of 8 h each grab continuous ileal digesta collection. In Exp. 2, 9 barrows (24.9 kg BW) were assigned to the 4 diets in a repeated 9 × 4 Youden square design to give 9 replicates per diet. In each period, pigs were adjusted to diets for 7 d followed by 5 d of fecal grab sample collection. The analyzed CP was 30.1, 42.3, and 43.1% (on a DM basis) in the CON, HP-A, and HP-B samples, respectively; the corresponding values for GE were 5,067, 5,425, and 5,407 kcal/kg DM, respectively. The concentrations of fat and NDF were comparable among the DDGS samples. Except for Arg and Val, the SID values for indispensable AA were not different ( > 0.05) among the 3 DDGS samples. However, HP samples had higher ( < 0.05) SID values for indispensable AA and CP compared with the CON sample. The SID of Lys content was 4.9, 6.1, and 7.4 g/kg DM for the CON, HP-A, and HP-B, respectively; the corresponding values for Met and Thr were 4.6, 6.8, and 7.4 g/kg DM, respectively, and 6.8, 9.5, and 10.7 g/kg DM, respectively. The DE content in HP samples was greater ( < 0.01) than in the CON (3,614, 4,494, and 4,555 kcal/kg DM for the CON, HP-A, and HP-B, respectively). In conclusion, HP DDGS had higher SID content of AA and DE compared with the CON.

Nutrient composition, digestible and metabolizable energy content, and prediction of energy for animal protein byproducts in finishing pig diets1

An industry survey and animal experiment were conducted to evaluate compositional variability and DE and ME content of animal protein byproducts and to generate equations to predict DE and ME content based on chemical analysis. For the 220 samples collected, the greatest concentration of CP was observed in blood meal (BM) and the least in meat and bone meal (MBM) and the greatest concentration of ether extract was in meat meal and the least in BM, with ash content greatest in MBM and least in BM, with Ca and P levels being 36.1 and 16.3% of the ash content, respectively. For the balance experiment, a corn-soybean meal basal diet was used with test diets formulated by mixing 80% of the basal diet with 20% of the animal protein byproduct, except for BM, which was included at 10 and 20% of the test diets. Ten groups of 24 gilts (92.5 ± 7.4 kg final BW) were randomly assigned to the test or basal diet within each group, resulting in 16 replications per animal protein byproduct or basal diet, except for BM determinations (20 replications). Gilts were placed in metabolism crates and offered 2.4 kg daily of their assigned diet for 13 d, with total collection of feces and urine during the last 4 d. Gross energy in the diets, feces, and urine was used to calculate the DE and ME content of each ingredient by the difference procedure, using DE and ME of the basal diet as a covariate among groups of pigs. The DE content of the animal protein byproducts ranged from 5,367 to 2,567 kcal DE/kg DM, and ME ranged from 4,783 to 2,340 kcal ME/kg DM. Using all animal protein byproducts, the best-fit equations were as follows: DE (kcal/kg DM) = -2,468 + (1.26 × GE, kcal/kg DM), with of 0.84, SE = 390, and < 0.01, and ME (kcal/kg DM) = -2,331 + (1.15 × GE, kcal/kg DM), with of 0.86, SE = 327, and < 0.01. The apparent total tract digestibility (ATTD) of Ca and P were also determined using the difference procedure, with the average ATTD of Ca and P for the animal protein byproducts, excluding BM and feather meal, being 27.1 and 39.1%, respectively. These data indicate that DE and ME substantially varied among the animal protein byproducts and sources and that a variety of nutritional components can be used to accurately predict DE and ME for finishing pigs. In addition, it appears that high dietary inclusion rates of animal protein byproducts may result in low ATTD estimates of Ca and P, which may be due to excessive concentrations of total Ca and P affecting digestibility.

Digestibility of energy and lipids and oxidative stress in nursery pigs fed commercially available lipids.

An experiment was conducted to evaluate the impact of lipid source on GE and ether extract (EE) digestibility, oxidative stress, and gut integrity in nursery pigs fed diets containing 10% soybean oil (SO), choice white grease (CWG), palm oil (PO), distillers' corn oil with approximately 5% FFA (DCO-1), or distillers' corn oil with approximately 10% FFA (DCO-2). Fifty-four barrows weaned at 28 d of age were fed a common starter diet for 7 d, group fed their respective experimental diets for an additional 7 d, and then moved to metabolism crates and individually fed their respective diets for another 10 d. Following this period, a 4-d total fecal and urine collection period was used to determine apparent total tract digestibility (ATTD) of GE and EE and to determine the DE and ME content of each lipid source (11.03 ± 0.51 kg final BW). Following the last day of fecal and urine collection, pigs were given an oral dose of lactulose and mannitol and fed their respective experimental diets with urine collected for the following 12 h. A subsequent urine collection occurred for 5 h to determine thiobarbituric acid reactive substances (TBARS) and isoprostane (IsoP) concentrations. Following this urine collection, serum was obtained and analyzed for TBARS and endotoxin concentrations. Soybean oil had the greatest ( < 0.05) DE (9,388 kcal/kg) content compared with DCO-1, DCO-2, CWG, and PO (8,001, 8,052, 8,531, and 8,293 kcal/kg lipid, respectively). Energy digestibility was greatest for SO compared with the other lipid sources ( < 0.05). The ATTD of EE averaged 85.0% and varied slightly (84.4 to 85.6%) among treatments. Differences in ME content among lipids were similar to those reported for DE, with ME values for DCO-1, DCO-2, CWG, PO, and SO being 7,921, 7,955, 8,535, 8,350, and 9,408 kcal/kg lipid, respectively. Metabolizable energy as a percentage of DE did not differ among lipid sources. Pigs fed lipid diets had greater ( < 0.05) serum TBARS compared with pigs fed the control diet, but no differences were observed in urinary TBARS excretion among the lipid treatments. Urinary IsoP excretion differed among treatments ( < 0.01) but was highly variable (34.0 to 104.6 pg). However, no differences were observed among treatments for the urinary lactulose:mannitol ratio and serum endotoxin. These results indicate that DE and ME content of SO are greater than that of other lipid sources evaluated, but feeding these lipids has no effect on gut integrity while producing variable effects on oxidative stress.