Content Of Antinutritional Factors Research Articles

Chemical proximate composition, antinutritional factors content, and antioxidant capacity of anatomical seed fractions of Moringa oleifera

For rational use of moringa seeds and its anatomical fractions (kernel, shells, and wings), the objective of the present investigation was to analyze their chemical composition, antinutritional factors content, and antioxidant capacity. The seed kernel fraction represents 71.78% of the whole seed weight and possesses high contents of protein (32.19%) and lipids (38.72%), whereas seed shells and wings fractions are mainly composed of dietary fiber and carbohydrates. Phytate was the most abundant antinutritional factor detected in all fractions (604.0 mg PAE/100 g-1674.9 mg PAE/100 g), followed by total phenolics (218.5 mg GAE/100 g-386.9 mg GAE/100 g) and saponins (130.4 mg DE/100 g-197.4 mg DE/100 g). Moringa seeds fractions showed a high antioxidant capacity. The kernel fraction exerts the highest activity (15 251.90 μmol TE/100g), followed by shells (8 499.50 μmol TE/100g) and wings (5 878.90 μmol TE/100g). Moringa seed kernel fraction seems to be a promising source of protein. All anatomical seed fractions of moringa are a reservoir of dietary fiber and antioxidants.

Wild Edible Plant Nutritional Contribution and Consumer Perception in Ethiopia.

The scarcity, high cost, and unreliable supply of healthy food in developing countries have resulted in the search for cheap and alternative sources of healthy and nutritious food. Wild edible plants (WEPs) are one of the alternative sources of healthy and nutritious food, and they are crucially important in supporting the global food basket in all parts of the world in general and in sub-Saharan Africa in particular. These wild edible plants have played a significant role in supplying food and nutritional requirements and increasing the health status of poor communities in many rural parts of the world. In Ethiopia, rural communities use wild edible plants as a means of survival especially during times of drought and famine and during other forms calamities and crises. Wild edible plants have high nutritional content, including proteins, vitamin B2, and vitamin C, which can be used as alternatives to conventional plant-based human diets. The available literature has revealed that some wild edible plants also have medicinal properties. Even though wild edible plants are important for food security, they are usually overlooked and perceived as food for poor families. This review indicates that fruits are the most commonly used WEPs, both for consumption and medicinal value, and most plant parts are eaten directly in raw forms. This review focuses on the commercial exploitation of wild edible plants as a source of dietary supplements and alternative medicines and as a means to generate income; it also focuses on consumer perception toward wild edible plants in Ethiopia. Despite easy accessibility and availability, the consumption of wild edible plants is challenged by numerous factors. This review suggests that nutrition policies have to promote the utilization of wild edible plants as one pillar of food and nutrition security. Bioactive compound contents and antinutritional factor contents of wild and medicinal plants need further investigation.

Effect of Sprouting on the Anti-Nutritional Factors in Vigna radiate (L), Macrotyloma uniflorum (L), Vigna mungo (L) And Cicer arietinum (L)

The aim was to ascertain whether the process of sprouting reduced the concentration of antinutritional factors in Vigna radiata, Macrotyloma uniflorum, Vigna mungo (L) and Cicer arietinum (L) belonging to Fabaceae family. Nutrition is the most important concern in everyones diet plan and is also a need to have balanced food. Care must also be taken towards the anti-nutritional factors that inhibit the absorption of nutrients thereby decreasing the nutritional value of the food. The pulses were screened for major specific anti nutrients (tannins, phytic acid, alkaloids and cyanogenic glycosides) before and after sprouting. The process of sprouting decreased the concentration of tannins, phytic acids and cyanogenic glycosides whereas the alkaloid concentration was increased.

Thermomechanical and enzyme-facilitated processing of soybean meal enhanced in vitro kinetics of protein digestion and protein and amino acid digestibility in weaned pigs.

Soybean meal (SBM) contains anti-nutritional factors (ANF) that may limit kinetics and total extent of protein digestion in pigs. This study evaluated the effects of thermomechanical and enzyme-facilitated (TE) processing on in vitro kinetics of crude protein (CP) digestion and CP and amino acid (AA) digestibility in weaned pigs. Each batch of SBM (48% CP) was divided into two parts: non-processed SBM as control vs. thermomechanical and enzyme-facilitated processed soybean meal (TES) as the experimental group. For digestion kinetics, samples (three batches of non-processed SBM vs. TES) were incubated in triplicate sequentially with pepsin at pH 3.5 for 1.5 h (stomach phase) and subsequently with pancreatin and bile extract at pH 6.8 for 0, 0.5, 1, 2, 4, or 6 h (small intestine phase). Protein was classified into CPfast, CPslow, and CPresistant corresponding to CP digested within the first 0.5 h, from 0.5 to 4 h, and after 4 h plus undigested CP, respectively. Eight weaned barrows (Large White × Duroc, 9.43 ± 0.40 kg) were surgically fitted with a T-cannula at the terminal ileum. Pigs were randomly assigned to a Youden square with three diets over four periods. The three diets were an N-free diet and two diets using 40% SBM or TES as the sole source of AA with Cr2O3 as an indigestible marker. Each period included sequentially a 5-d adaptation, 2-d collection of feces, and 2-d collection of ileal digesta. The TE processing reduced ANF content in TES by 91% for lectin, 22% for trypsin inhibitor activity, 75% for β-conglycinin, and 62% for glycinin compared with SBM. In vitro, TE processing increased (P < 0.05) digested CP by 5.6% and enhanced the kinetics of CP digestion by tending to increase (P = 0.056) CPfast by 25% and reducing (P < 0.05) CPslow and CPresistant by 48% and 11%, respectively. In pigs, TE processing increased (P < 0.05) apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP in TES by 2.3% and 2.1%, respectively. The TE processing increased (P < 0.05) AID and SID of all AA up to 3.3%, except for AID of Pro and SID of Pro, Gly, and Cys. The TE processing did not change reactive Lys or Lys:CP but increased (P < 0.05) SID of Lys and reactive Lys by 3%. Combined, the greater in vitro digestion kinetics matched the greater in vivo AID and SID of CP in TES and lower ANF compared with SBM. Thus, TE processing created a protein source that is digested faster and to a greater extent than SBM, thereby lowering the chance of protein fermentation.

Impact of debittering and fermentation processes on the antinutritional and antioxidant compounds in Lupinus mutabilis sweet

Lupin is a nutritive grain, but its use is limited due to its high content of bitter alkaloids and other antinutritional factors, such as phytic acid, tannins, nitrates and trypsin inhibitors (TI), that have undesirable physiological effects. There is increasing interest in finding appropriate methods for reducing the antinutritional compounds in lupin. The objective of this research was to assess the efficacy of a biotechnological process, namely, fungal fermentation, as a debittering process relative to that of conventional aqueous thermal treatment (ATT). We evaluated the effects of these processes on the reduction of antinutritional compounds as well as their potential impacts on enhancing the beneficial antioxidant properties of lupin. Three varieties (INIAP-450, INIAP-451 and Criollo) of the Lupinus mutabilis species were studied. The application of ATT and fermentation with Rhizopus oligosporus caused decreases in the following antinutrients: nitrates (94.59%), tannins (82.10%), alkaloids (94%), urease activity (93.75%), phytic acid (70.06%) and trypsin inhibitors (76.76%). Ascorbic acid also decreased (79.72%). All values corresponded to the average in the three varieties evaluated. While the contents of phenols, carotenoids and the antioxidant capacity decreased by 96.83, 49.42 and 96.13%, respectively, due to the debittering process, solid fermentation promoted increases in these compounds and properties in the debittered grain.

Growth, nutrient retention, innate immune response, and intestinal morphology of juvenile, soy-naïve hybrid striped bass, Morone saxatilis x M. chrysops fed commercial-type, soy-based, ideal protein, fish meal replacement diets

To develop plant-based diets for hybrid striped bass (HSB), Morone chrysops × M. saxatilis, we evaluated six soy products in extruded, ideal protein, fish meal (FM) replacement diets fed to soy-naïve juveniles (3.72 ± 0.01 g initial weight) for up to 5 months. The products included soy protein concentrate (SPC), a conventional soybean meal (SBM) from Archer Daniels Midland (ADM), three non-genetically modified (non-GM) soybean varieties from the University of Arkansas (R05–1415, R05–1772, and R07–2001) with high protein and putatively reduced anti-nutritional factor (ANF) content, and Ozark soybeans, parent to the non-GM strains. The control diet included Special-Select® menhaden fish meal (MFM-S; Omega Protein) at 25.5% of diet and contained 40% digestible protein (DP) and 16% lipid. MFM-S was replaced by each soy product on a digestible protein basis and supplemented with Met, Lys, and Thr based on 40% HSB muscle AA profile. HSB sac fry were reared on soy-free diets to increase the likelihood of detecting any soy-induced performance issues when they were fed the test diets to satiation twice daily. Daily feed intake did not differ among treatments (2.2–2.5% of body weight/day). Fish fed the ADM diet were larger (58 g) and gained (1465%) more than those fed the control (51 g, 1251%); otherwise final weights (47-54 g) and weight gains (1153%–1355%) did not differ from the FM control. Gains in fish fed the SPC diet fell below those of fish fed the ADM, Ozark, and R0–1415 diets. Feed conversion ratios (FCR) were slightly poorer for fish fed the R05–1772 diet (1.09) compared to those fed the control (0.93); otherwise, FCR (0.97–1.01) did not differ among diets. The following responses also did not significantly differ among treatments: protein, energy, and AA retentions, whole body protein, moisture, and ash, muscle ratio, and hematological and innate immune parameters. There were slight differences among treatments in whole body lipid and energy, Glu and His content, and liver size. Challenge with Columnaris generally revealed no adverse effect of soy diet on time to death. In contrast to most fish studies, moderate inflammation was noted in proximal intestinal samples from soy-based diets after 73 days, whereas distal gut morphology did not differ among diets. However, no intestinal inflammation was found after a further 5 months of feeding test diets. Complete replacement of FM with various soybean varieties is possible in juvenile hybrid striped bass diets using ideal protein formulas with little impact on fish performance.

Standardization and Stabilization of Millet Milk by Enzyme and Its Physicochemical Evaluation

Millets are nutritionally rich and occupy an important place in the diet of people in many regions of the world. Although millets are nutritionally superior to cereals, their utilization as a food is mostly confined to the traditional consumers. So, the present study was undertaken to standardize millet milk from barnyard millet, little millet, kodo millet and finger millet by enzymatic extraction method. Aqueous extract of millet milk was treated with α amylase and pasteurized at 75ºC for 15 minutes. The pasteurized millet milk was evaluated for physical and nutritional parameters. Results showed that the physical properties of developed millet milk have met the requirement of plant-based milk in terms of viscosity (2.32±0.02 to 2.82±0.03). Protein content of millet milk varied from 1.38±.0.03 to 1.12±.0.02 g. Total polyphenols (205.72±0.13 mg/100 ml) and total antioxidant activity (81.64±1.77%) were high for finger millet milk and total flavonoid content was high for barnyard millet milk (96.25±1.88 mg/100 ml). Enzymatic treatment significantly reduced the anti-nutritional factor (phytic acid, tannin and trypsin inhibitor activity) content in millet milk. The enzymatically developed product had high In vitro protein (69.28±0.28 to 85.57±1.39%) and starch digestibility (69.75±0.56 to 63.36±0.12 mg maltose/g). From the results, it was concluded that the current approach provides a convenient way for the production of nutritionally sound millet milk at the household and industrial level.

Effect of Germination on Nutritional Composition and Functional Properties of Fenugreek (Trigonellafoenum-graecum Linn) Seed Flour

This study evaluated the effect of germination on the chemical composition and functional properties of fenugreek seed flour. The study was conducted at Addis Ababa Institute of Technology in Food Engineering laboratory. Fenugreek seed was germinated for 0, 24, 48 and 72 hrs. Germinated fenugreek flour had higher crude protein 29.89% in fenugreek flour germinated for 72 hrs followed by 29.36% in fenugreek flour germinated for 48 hrs and 27.42% in raw fenugreek flour. The crude fiber ranged from 11.349 to 14.70%. But as the time of germination increased the crude fiber content slightly decreased. Fenugreek flour germinated for 48 hrs proved to be more beneficial in most mineral content than the rest of the germination periods (p&lt;0.05). Germinated fenugreek flour for 72 hrs had significantly lower contents of anti-nutritional factors. The phytate content decreased significantly (p&lt;0.05) from 64.22 to 18.99 with 53.96% reduction. The study showed that fenugreek flour germinated for 72h proved to be the best for highest nutrient content. The reduction of bulk density further increased as germination time increased. The control fenugreek flour have higher amount of water absorption capacity (401.86%). On germination the water absorption capacity decreased to 250.07, 248.13, and 245.15% at 24, 48, 72 hrs germination, respectively. The processed fenugreek flour had a higher oil absorption capacity in the range of 346.13-398.40 ml/g, which was significantly (p&lt;0.05) higher than that of control flour. Therefore, the use of processed fenugreek seed flour can be exploited in functional foods as well as a therapeutic agent on a regular basis.

Antinutritional Factors and Aminoacids Content Comparison with Different Processing Methods of Balanites aegyptiaca in Formulated Broiler Diets

A study was conducted to evaluate the effect of processing methods on the antinutrients and amino acids composition of Balanites aegyptiaca. The durations for soaking, roasting and fermentation were 0, 24, 48, 72 and 96 hours to determine the optimal duration of inclusion of processed Balanites aegyptica. Raw and processed samples were analysed for amino acids composition and anti-nutritional factors.The trial showed a linear increase in processing duration resulted into a decrease in the concentration of antinutritional factors. Raw Balanites aegyptica had higher concentration of concentration of amino acid than the soaked and boiled Balanites aegyptica. Soaking, fermentation and boiling up to 96 hours reduced the antinutritional components of Balanite aegyptica. Percentage reduction of anti-nutrients in Balanites aegyptica. fruit meal was best at 96 hours of soaking and fermentation and 60 minutes of boiling and roasting. All anti-nutrients in Balanites aegyptiaca fruit meal showed remarkable reduction post processing. In all processing methods, results showed that anti-nutrients in the Balanites aegyptiaca reduced up to the maximum durations of processing (96 hours and 60 minutes). It was therefore advisable to include Balanites aegyptiaca upto 96 hours in the diets of broiler birds.

Evaluation of the standardized ileal digestibility of amino acids of rapeseed meals varying in protein solubility for Pekin ducks

This study was conducted to determine whether protein solubility (PS) of rapeseed meals (RSM) can affect standardized ileal amino acid digestibility (SIDAA) in meat ducks. A total of 1,168, 14-days-old ducks were randomly allotted to 23 treatments (6 cages per diet, 8 ducks per cage) and 1 nitrogen-free diet treatment (8 cages, 8 ducks per cage) based on body weight. The 23 experimental diets consisted of a corn–soybean meal basal diet, and 22 diets containing 15% RSM: 85% basal diet. Titanium dioxide (0.5%) was included in all diets as an indigestible marker. On day 18, all ducks were euthanized by carbon dioxide asphyxiation and digesta samples from the ileum. The contents of PS, ether extract (EE), glucosinolate, isothiocyanate, and oxazolidine were significantly different (P < 0.05) in the 22 RSM, with the CV being 52.62, 49.23, 86.84, 90.19, and 81.98%, respectively. The content of lysine (Lys) and methionine in the 22 RSM samples ranged from 1.03 to 2.71% (CV 24.19%) and from 0.33 to 0.65% (CV 15.17%), respectively. The SIDAA, except for leucine (Leu) and tyrosine, of the 22 RSM samples varied significantly (P < 0.05). A positive correlation was observed (P < 0.05) between PS and standardized ileal digestibility (SID) of Lys, isoleucine, valine, phenylalanine, histidine, serine, cysteine, and tyrosine. The R2 value of multiple linear regression equations for predicting the SID of amino acids (AA) was best for Lys (R2 = 0.958 using dry matter, crude protein, EE, crude fiber, acid detergent fiber, and PS) and least significant for Leu (R2 = 0.348 using crude fiber and ash) with intermediate values for other AA (R2 = 0.359–0.837, P < 0.05). These results suggest that PS varying from 15.06 to 98.08%, also varied considerably in the proximate nutrient content, AA composition, and antinutritional factor content, which was reflected in considerable differences in the duck's SID of AA in RSM. Therefore, PS value can partly reflect the quality of RSM.

Use of Fermented Hemp, Chickpea and Milling By-Products to Improve the Nutritional Value of Semolina Pasta.

A biotechnological approach including enzymatic treatment (protease and xylanase) and lactic acid bacteria fermentation has been evaluated to enhance the nutritional value of semolina pasta enriched with hemp, chickpea and milling by-products. The intense (up to circa, (ca.) 70%) decrease in the peptide profile area and (up to two-fold) increase in total free amino acids, compared to the untreated raw materials, highlighted the potential of lactic acid bacteria to positively affect their in vitro protein digestibility. Fermented and unfermented ingredients have been characterized and used to fortify pasta made under pilot-plant scale. Due to the high contents of protein (ca. 13%) and fiber (ca. 6%) and according to the Regulation of the European Community (EC) No. 1924/2006 fortified pasta can be labelled as a “source of fiber” and a “source of protein”. The use of non-wheat flours increased the content of anti-nutritional factors as compared to the control pasta. Nevertheless, fermentation with lactic acid bacteria led to significant decreases in condensed tannins (ca. 50%), phytic acid and raffinose (ca. ten-fold) contents as compared to the unfermented pasta. Moreover, total free amino acids and in vitro protein digestibility values were 60% and 70%, respectively, higher than pasta made only with semolina. Sensory analysis highlighted a strong effect of the fortification on the sensory profile of pasta.

Proximate components, minerals, amino acids and some anti-nutrients in processed false yam seed meals: Potential benefits for poultry nutrition

Abstract This study investigated the effect of sequential processing techniques (water treatment, chemical treatment and blanching) of false yam seed meal (FYSM) on its proximate composition, mineral and amino acid concentrations and anti-nutritional compounds. Matured false yam (Icacina oliviformis) fruits were harvested and seeds extracted. Five different FYSM samples were prepared. One sample was prepared by crushing fresh false yam seed and sun-drying (Un_T). Four other samples were crushed, each soaked in water (1:2; w/v) for 12 days with water replaced every 3 days. Afterwards, each sample was re-soaked in 1 M concentration of urea (Urea_T), sodium chloride (NaCl_T), sodium hydroxide (NaOH_T) and potassium hydroxide (KOH_T), respectively, for 24 h, washed, blanched and sun-dried to a moisture content of 12%. Sample analysis revealed high dry matter [DM; 89% (NaOH_T) – 93% (KOH_T)] and carbohydrate [71.4% (Un_T) – 83.9% (NaCl_T)] content but a reduction of crude protein from 13.2% (Un_T) to 2.2% (KOH_T). Mineral analysis showed a reduction in Ca from 280 (Un_T) to 24 mg/kg DM (NaCl_T) and in Mg from 52.8 (Un_T) to 13.29 mg/kg DM (Urea_T) but an improvement in K from 110.6 (Un_T) to 368.8 mg/kg DM (KOH_T), in Na [from 558 (Un_T) to 989 mg/kg DM (NaCl_T)] and total P [from 0.31 (Un_T) to 2.37 mg/kg DM (Urea_T)]. Amino acid composition of FYSM was negatively affected by all treatments. Concentrations of anti-nutritional factors, namely total terpenes and saponins, were reduced by 82% (KOH_T) to 92% (Urea_T) and by 68% (KOH_T) to 77% (NaCl_T), respectively. In vitro gas production per 380 mg of sample was similar across treatments. Reducing anti-nutritional factors by sequential use of simple processing methods with easily available chemicals offers the potential to use FYSM in livestock diets. Yet, as some nutrient concentrations are negatively affected, in vivo trials are needed to determine the nutritional value of treated FYSM.

Beneficial influences of dietary Aspergillus awamori fermented soybean meal on oxidative homoeostasis and inflammatory response in turbot (Scophthalmus maximus L.)

High levels of soybean meal (SBM) in aquafeed leads to detrimental inflammatory response and oxidative stress in fish. In the present study, fermentation with Aspergillus awamori was conducted to explore the potential effects on improving the nutritional quality of soybean meal and the health status of turbot. A 63-day feeding trial (initial weight 8.53 ± 0.11 g) was carried out to evaluate the utilization of fermented soybean meal (FSM) by juvenile turbot. 0% (FM, control), 30% (S30, F30), 45% (S45, F45), and 60% (S60, F60) of fish meal were replaced with SBM or FSM, respectively. As the results showed, fermentation significantly reduced the contents of anti-nutritional factors in SBM, including raffinose (−98.8%), glycinin (−98.5%), β-conglycinin (−97.4%), trypsin inhibitors (−80%) and stachyose (−80%). A depression of fish growth performance and activities of superoxide dismutase and lysozyme were observed in S45 and S60 groups, while these inferiorities were only observed in F60 group. Meanwhile, fermentation also improved the heights of enterocytes and microvillus significantly in the F45 and F60 groups compared with those in SBM. An induced expression of anti-inflammatory cytokine transforming growth factor-β and depression of pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in the distal intestine were observed in the F45 and F60 groups. Taken together, this study indicated that fermentation with Aspergillus awamori could improve the replacement level with soybean meal from 30% to 45% in turbot.

Increasing hybrid rye level substituting wheat grain with or without enzyme on growth performance and carcass traits of growing-finishing barrows and gilts.

: New European, fall-planted hybrid rye grown in western Canada is more resistant to ergot and fusarium and has lower content of anti-nutritional factors than common rye. We evaluated the effect of feeding increasing hybrid rye level substituting wheat grain and non-starch polysaccharide (NSP) enzyme inclusion in diets fed to growing-finishing pigs raised under western Canadian commercial conditions. In total, 1,008 pigs (~44 kg body weight [BW]) housed in 48 pens by sex, 21 pigs per pen, were fed diets with one of three rye (var. KWS Bono; KWS LOCHOW GMBH) inclusion levels substituting wheat grain: low (L; one-third of wheat replaced), medium (M; two-thirds of wheat replaced), or high (H; most wheat replaced), either without (WO) or with (W) enzyme inclusion (280 units of β-glucanase and 900 units of xylanase per kilogram feed; Endofeed W DC; GNC Bioferm) over four growth phases (Grower 2: d 0 to 22, Grower 3: d 23 to 42, Finisher 1: d 43 to 63, Finisher 2: d 64 to slaughter). Pen BW, feed added, and orts were measured on d 0, 22, 42, 63, 76, 91, and at slaughter weight (130 kg). Warm carcasses were weighed and graded (Destron). BW was not affected by either increasing hybrid rye level substituting wheat grain or enzyme inclusion throughout the trial. For the entire trial (d 0 to 76), pigs fed increasing hybrid rye level substituting wheat grain had decreased (P < 0.050) average daily feed intake (ADFI; L 3.05, M 2.98, H 2.91 kg/d) and average daily weight gain (ADG; L 1.01, M 1.00, H 0.97 kg/d). Enzyme inclusion did not affect ADFI but tended (P = 0.080) to increase ADG (WO 0.98, W 1.00 kg/d). Enzyme inclusion improved (P < 0.050) gain-to-feed ratio only in pigs fed the H rye level. Most carcass traits were not affected by either increasing hybrid rye level substituting wheat grain or enzyme inclusion. Increasing dietary hybrid rye level substituting wheat grain increased (P < 0.001) cost per tonne of feed (L 240.28, M 241.28, H 242.20 Can$/kg), but did not affect feed cost per pig or per kilogram BW gain. Enzyme inclusion increased (P < 0.001) cost per tonne of feed (WO 240.36, W 242.15 Can$/kg), but feed cost per pig (WO 82.14, W 80.44 Can$ per pig) and per kilogram BW gain (WO 0.96, W 0.94 Can$/kg gain) were reduced (P < 0.050). In conclusion, fall-planted hybrid rye can completely replace wheat grain in grower-finisher pig diets without affecting feed efficiency, feed cost per pig or feed cost per kilogram BW gain. Inclusion of NSP enzyme would be recommended for diets including high rye levels to improve feed efficiency and ADG.

Anti-nutritional and toxicological properties of Balanites aegyptiaca: a review

Edible wild plants and their products have played a substantial role in tackling the increasing gap between population growth and food supply. This article reviewed the scientific information on the anti-nutritional and toxicological properties of Balanites aegyptiaca (desert date). Systematic literature search shows the plant to contain saponin, tannins, oxalate and phytic acid that have been known to have adverse effects on health through inhibition of biomolecules digestion, important enzymes, iron, calcium and zinc absorption. From nutritional point of view, anti-nutrients are substances that interfere with normal growth and uptake of nutrients, reproduction and health, and when consumed regularly in amount found in a normal component of diet is considered harmful and toxic. Knowledge regarding ways and techniques to lower the content of anti-nutritional factors in B. aegyptiaca is needed for health and well-being of the population.Keywords: B. aegyptiaca, toxicity, nutrition

Improvement of protein content and decrease of anti-nutritional factors in olive cake by solid-state fermentation: A way to valorize this industrial by-product in animal feed

The present work investigates the bioconversion of the olive cake (OC) generated by olive oil industries in Morocco through solid-state fermentation using selected filamentous fungi to increase its nutritional values for subsequent valorization as ruminants feed. The fungi, namely Beauveria bassiana, Fusarium flocciferum, Rhizodiscina cf. lignyota, and Aspergillus niger were cultured on OC for 15 days. Chemical composition as well as enzymes activities were determined. Results showed (i) an increase in protein content of up to 94% for treated OC and (ii) significant (P < 0.05) decreases of phenolic compounds, up to 43%, 70% and 42% for total phenolic content, total flavonoids content, and total condensed tannins, respectively. Moreover, the RP-HPLC analysis of fermented OC confirmed the degradation of individual phenolic compounds by the strains. These findings demonstrate that F.flocciferum and Rhizodiscina cf. lignyota are efficient enzymes producers leading to a nutritive enhancement of this by-product.

Effect of Baobab (Adansonia digitata) Seed Meal on Performance Characteristics of Rabbit Does

The study assessed the effect of baobab (Adansonia digitata) seed meal (BSM) on some growth and reproductive parameters of rabbit does. A total of 24 adult does (age range) were used, the rabbits were grouped into four treatments having six replicates with each replicate consisting of one doe. Treatment 1 (T1) served as the control, treatment 2 (T2) had 5% BSM, treatment 3 (T3) had 10% BSM and treatment 4 (T4) had 15% BSM. The study lasted for 10 weeks.&#x0D; The antinutritional factor content of the diet increased with BSM inclusion. Tannin, saponin and alkaloid content in the control diet was 0.27, 260.00 and 0.86 mg/100 g respectively, while the diet with 15% BSM (T4) had 0.0055 mg/g (tannin), 7.73 mg/g (saponin) and 0.022 mg/g (alkaloid). However, the pure BSM had 0.0017 mg/g (tannin), 130.00 mg/kg (saponin) and 0.03 mg/g (alkaloid). Feed intake and weight gain were significantly (P&lt;0.05) higher for does in T2 compared to the control diet. Does on T2 converted their feed better compared to does on T3 and T4 with 16.93 and 16.95 respectively. The weight change during gestation revealed that does in T2 had a significant (P&lt;0.05) weight increase of 150.00 g compared with the control with a weight change of 137.00g. However, a weight loss of -30.00 g was observed for does fed T4 (15% BSM). No significant (P&gt;0.05) difference was observed in the litter size of does fed different diets with means ranging from 5.33 to 6.00, although the highest birth weight of kindles was observed in T2 (5% BSM) but was not significantly (P&gt;0.05) different from kindles of other treatments.&#x0D; It is concluded from the result of this study that inclusion of 5% ADSM in the diet of does did not result in any significant weight loss. However, inclusion levels above 5% posed a threat to the growth performance and reproductive attributes of does.

Polymorphisms in cyanogenic glucoside and cyano‐amino acid content in natural accessions of common vetch (Vicia sativa L.) and selection for improved agronomic performance

AbstractCommon vetch (Vicia sativa L.) is an important annual forage legume. It is used as a cover crop, green manure, pasture legume and for silage and hay production. Its seeds can be used as a source of highly digestible protein and minerals in animal diets. However, their utilization as a feedstuff for monogastric animals is hindered by the fact that the seeds contain cyanogenic antinutritional factors that reduce their palatability. An effective utilization of V. sativa seeds as a successful monogastric feed stuff requires selection for higher protein availability and minimization of the cyanogenic antinutritional factors content. In this study, we selected one natural accession named Mjez Ibeb, from a collection of 25 accessions and cultivars, based on its superior agronomic performance and its naturally occurring genetic variation for cyanogenic traits. We investigated the genetic variation that exists for the cyanogenesis trait in more detail and analysed the seeds of 133 lines derived from accession Mjez lbeb. Of these, 40 naturally polymorphic lines that showed deficiencies in cyanogenesis and cyano‐amino acid content, were subsequently selected for detailed chemical analysis. Cyanogenic glucosides and cyano‐amino acid concentrations varied widely in the 40 lines. Multivariate analysis was performed and three lines (L16, L21, L18) with low content of cyanogenic compounds were identified.

Gross visceral organs morphometry and carcass quality in broiler chicken fed Tannia (Xanthosoma sagittifolium) cocoyam

Tannia cocoyam meals (CCYM) as substitutes for maize were evaluated in a six week feeding trial, in order to determine and compare effects of dietary inclusion at 100% of tannia cocoyam and the levels of antinutritional factors present. Ninety-six broiler chickens at four weeks of age were randomly allotted in groups of 12 to one of the following diets. The dietary treatments contain 0, 25, 50 and 100% CCYM of Tannia which comprised of raw sundried and boiled sundried forms. Proximate analyses of the test ingredient and that of maize was conducted. Antinutritional factor analyses of the test ingredients were also conducted. Carcass quality evaluation was also determined only on control groups and 100% (cocoyam inclusion). The results of proximate compositions indicated that tannia is higher in crude fibre, ash and nitrogen free extract. The weight of crop on raw tannia (31.70) was significantly lower (p&lt;0.05) than that of the control diet (32.45). The live weight, dressed weight and eviscerated weight and all other cut parts for both raw and boiled CCYM were similar (p&gt;0.05) to control. The weights of crop and intestine on 100% CCYM diets differed significantly (p&lt;0.05) with lower values on the control (32.45 and 112.08 respectively) and higher values on boiled sundried tannia (53.74 and 132.30 respectively). Significantly higher levels (p&lt;0.001) of anti-nutritional factors were observed on raw sundried tannia than the boiled sundried tannia. The carcass yield and organs weight were significantly higher (p&lt;0.05) on boiled sundried tannia as compared to that of raw sundried. Thus, better feed utilization on boiled tannia diets due to higher feeding value than raw sundried cocoyam. Therefore, boiled tannia could replace maize at 100% inclusion levels without any adverse effects on carcass characteristics, but lower levels of raw tannia is recommended due to higher content of antinutritional factors.

Effects of Replacing Fishmeal with Soybean Products in Fish and Crustaceans Performance

Fish meal (FM) is a principal dietary protein source in aquafeed. The rapid development of aquaculture resulted in high demand and a shortage of (FM) supply. The reason mentioned above has led to a search for an alternative protein sources as (FM) replacement in aquafeeds. Amongst plant protein ingredients, soybean meal (SB) is suggested as the most nutritive plant protein source but some contains high concentration of anti-nutritional factors. Among most soybean products compared with FM, soybean protein concentrate (SPC) is most suitable to replace fishmeal in aquafeed because of its high protein content and better amino acid profile than other soybean products such as soybean meal (SBM). Moreover, more SPC can be used to fully or partially replace FM as compared to SBM without adverse effects on growth performance and health condition in fish and crustaceans. This review reveals that SPC has positive effect on fish and crustaceans performance, gene expression and pathways. This information will help reduce the use of fishmeal by replacing it with SPC in aquafeed and also help to improve growth and health of fish and crustaceans.