Stories from a Ming Collection is a model work of "parodyscript" in the late Ming Dynasty. With the help of the rich plot and vivid narrative techniques, the author shows the moral values of the society at that time and the ordinary people's daily human relations. As an ancient north-south traffic artery, the Grand Canal had an important influence on the working ways and customs of the people along the coastland formed a canal culture with distinctive features. Based on the Canal story in Stories from a Ming Collection, this paper sorts out and summarizes the presentation of Canal Culture in the text and then analyzes the cultural elements contained in it, such as traffic, business and folk customs, etc., providing reference for understanding and carrying forward the contemporary value of the Grand Canal culture.