Residues of antibiotics applied in chicken husbandry can be found in eggs for human consumption. To prevent this issue and avoid adverse effects on consumers' health, such as allergies and antimicrobial resistance (AMR), maximum residue limits (LMR) have been established by the European Commission. The aim of this study was to develop a method to extract, detect and quantify antibiotics in eggs, using UHPLC-TOF-MS for semi-quantitative screening and UHPLC-QTRAP-MS/MS for quantitative confirmation, following the performance criteria established by Commission Regulation (EU) Nº 2021/808 for analytical method validation. Different extraction methods were analyzed, with liquid-liquid extraction (LLE) based on acetonitrile (ACN) and ethylenediaminetetraacetic acid (EDTA), proving to be the most efficient method for extraction of antibiotic residues from eggs. UHPLC-TOF-MS and UHPLC-QTRAP-MS/MS were validated for 46 compounds, including penicillin, cephalosporins, tetracyclines, quinolones, macrolides, sulfonamides, and other antibiotics. After validation, robustness of both methods was evaluated by analyzing ten egg samples from backyard production and supermarkets. Erythromycin residues were detected in two eggs from backyard production; residues of up to seven antibiotics were detected in two samples of supermarket eggs. Nonetheless, all positive samples were below the established MRL. The developed methods will be a useful tool to monitor possible contamination in eggs, as they allow the simultaneous detection of several molecules. Furthermore, this methodology can be optimized to reduce the relative matrix effect, extended to a larger group of molecules, include other types of drugs, and be validated for other matrices of animal origin.