Contamination Of Coastal Waters Research Articles

Follow-Up of Norovirus Contamination in an Oyster Production Area Linked to Repeated Outbreaks.

A production area repeatedly implicated in oyster-related gastroenteritis in France was studied for several months over 2years. Outbreaks and field samples were analyzed by undertaking triplicate extractions, followed by norovirus (NoV) detection using triplicate wells for genomic amplification. This approach allowed us to demonstrate that some variabilities can be observed for samples with a low level of contamination, but most samples analyzed gave reproducible results. At the first outbreak, implicated oysters were collected at the beginning of the contamination event, which was reflected by the higher NoV levels during the first month of the study. During the second year, NoV concentrations in samples implicated in outbreaks and collected from the production area were similar, confirming the failure of the shellfish depuration process. Contamination was detected mainly during winter-spring months, and a high prevalence of NoV GI contamination was observed. A half-life of 18days was calculated from NoV concentrations detected in oysters during this study, showing a very slow decrease of the contamination in the production area. Preventing the contamination of coastal waters should be a priority.

Into the deep: Evaluation of SourceTracker for assessment of faecal contamination of coastal waters

Faecal contamination of recreational waters is an increasing global health concern. Tracing the source of the contaminant is a vital step towards mitigation and disease prevention. Total 16S rRNA amplicon data for a specific environment (faeces, water, soil) and computational tools such as the Markov-Chain Monte Carlo based SourceTracker can be applied to microbial source tracking (MST) and attribution studies. The current study applied artificial and in-laboratory derived bacterial communities to define the potential and limitations associated with the use of SourceTracker, prior to its application for faecal source tracking at three recreational beaches near Port Phillip Bay (Victoria, Australia). The results demonstrated that at minimum multiple model runs of the SourceTracker modelling tool (i.e. technical replicates) were required to identify potential false positive predictions. The calculation of relative standard deviations (RSDs) for each attributed source improved overall predictive confidence in the results. In general, default parameter settings provided high sensitivity, specificity, accuracy and precision. Application of SourceTracker to recreational beach samples identified treated effluent as major source of human-derived faecal contamination, present in 69% of samples. Site-specific sources, such as raw sewage, stormwater and bacterial populations associated with the Yarra River estuary were also identified. Rainfall and associated sand resuspension at each location correlated with observed human faecal indicators. The results of the optimised SourceTracker analysis suggests that local sources of contamination have the greatest effect on recreational coastal water quality.

Synthetic ultraviolet light filtering chemical contamination of coastal waters of Virgin Islands national park, St. John, U.S. Virgin Islands

Contamination of surface waters by synthetic ultraviolet light (UV) filtering chemicals is a concern for the Virgin Islands National Park (VINP). Discrete water samples were collected from VINP bays to determine UV filter chemical presence in the coastal waters. Spatial distribution and the potential for partitioning between subsurface waters and the sea surface microlayer (SML) were also examined. The UV filter chemicals 4-methylbenzylidene camphor, benzophenone-3, octinoxate, homosalate, and octocrylene were detected at concentrations up to 6073ng/L (benzophenone-3). Concentrations for benzophenone-3 and homosalate declined exponentially (r2=0.86 to 0.98) with distance from the beach. Limited data indicate that some UV filter chemicals may partition to the SML relative to the subsurface waters. Contamination of VINP coastal waters by UV filter chemicals may be a significant issue, but an improved understanding of the temporal and spatial variability of their concentrations would be necessary to better understand the risk they present.

Modeling of coastal water contamination in Fortaleza (Northeastern Brazil).

An important tool in environmental management projects and studies due to the complexity of environmental systems, environmental modeling makes it possible to integrate many variables and processes, thereby providing a dynamic view of systems. In this study the bacteriological quality of the coastal waters of Fortaleza (a state capital in Northeastern Brazil) was modeled considering multiple contamination sources. Using the software SisBaHiA, the dispersion of thermotolerant coliforms and Escherichia coli from three sources of contamination (local rivers, storm drains and submarine outfall) was analyzed. The models took into account variations in bacterial decay due to solar radiation and other environmental factors. Fecal pollution discharged from rivers and storm drains is transported westward by coastal currents, contaminating strips of beach water to the left of each storm drain or river. Exception to this condition only occurs on beaches protected by the breakwater of the harbor, where counterclockwise vortexes reverse this behavior. The results of the models were consistent with field measurements taken during the dry and the rainy season. Our results show that the submarine outfall plume was over 2 km from the nearest beach. The storm drains and the Maceió stream are the main factors responsible for the poor water quality on the waterfront of Fortaleza. The depollution of these sources would generate considerable social, health and economic gains for the region.

Biomonitoring of trace metal bioavailabilities to the barnacle Amphibalanus amphitrite along the Iranian coast of the Persian Gulf

The fouling barnacle Amphibalanus amphitrite is a cosmopolitan biomonitor of trace metal bioavailabilities, with an international comparative data set of body metal concentrations. Bioavailabilities of As, Cd, Cr, Cu, Fe, Mn, Pb, V and Zn to A. amphitrite were investigated at 19 sites along the Iranian coast of the understudied Persian Gulf. Commercial and fishing ports showed extremely high Cu bioavailabilities, associated with high Zn bioavailabilities, possibly from antifouling paints and procedures. V availability was raised at one port, perhaps associated with fuel leakage. Cd bioavailabilities were raised at sites near the Strait of Hormuz, perhaps affected by adjacent upwelling off Oman. The As data allow a reinterpretation of the typical range of accumulated As concentrations in A. amphitrite. The Persian Gulf data add a new region to the A. amphitrite database, confirming its importance in assessing the ecotoxicologically significant trace metal contamination of coastal waters across the world.

Survey of antibiotic-resistant bacteria isolated from bottlenose dolphins Tursiops truncatus in the southeastern USA

Contamination of coastal waters can carry pathogens and contaminants that cause diseases in humans and wildlife, and these pathogens can be transported by water to areas where they are not indigenous. Marine mammals may be indicators of potential health effects from such pathogens and toxins. Here we isolated bacterial species of relevance to humans from wild bottlenose dolphins Tursiops truncatus and assayed isolated bacteria for antibiotic resistance. Samples were collected during capture-release dolphin health assessments at multiple coastal and estuarine sites along the US mid-Atlantic coast and the Gulf of Mexico. These samples were transported on ice and evaluated using commercial systems and aerobic culture techniques routinely employed in clinical laboratories. The most common bacteria identified were species belonging to the genus Vibrio, although Escherichia coli, Shewanella putrefaciens, and Pseudomonas fluorescens/putida were also common. Some of the bacterial species identified have been associated with human illness, including a strain of methicillin-resistant Staphylococcus aureus (MRSA) identified in 1 sample. Widespread antibiotic resistance was observed among all sites, although the percentage of resistant isolates varied across sites and across time. These data provide a baseline for future comparisons of the bacteria that colonize bottlenose dolphins in the southeastern USA.

Emerging organic contaminants in coastal waters: Anthropogenic impact, environmental release and ecological risk

This study provides a first estimate of the sources, distribution, and risk presented by emerging organic contaminants (EOCs) in coastal waters off southwestern Taiwan. Ten illicit drugs, seven nonsteroidal anti-inflammatory drugs (NSAIDs), five antibiotics, two blood lipid regulators, two antiepileptic drugs, two UV filters, caffeine, atenolol, and omeprazole were analyzed by solid-phase extraction and liquid chromatography coupled to tandem mass spectrometry (SPE–LC–MS/MS). Thirteen EOCs were detected in coastal waters, including four NSAIDs (acetaminophen, ibuprofen, ketoprofen, and codeine), three antibiotics (ampicillin, erythromycin, and cefalexin), three illicit drugs (ketamine, pseudoephedrine, and MDMA), caffeine, carbamazepine, and gemfibrozil. The median concentrations for the 13 EOCs ranged from 1.47ng/L to 156ng/L. Spatial variation in concentration of the 13 EOCs suggests discharge into coastal waters via ocean outfall pipes and rivers. Codeine and ampicillin have significant pollution risk quotients (RQ>1), indicating potentially high risk to aquatic organisms in coastal waters.

Discharge of perfluorinated compounds from rivers and their influence on the coastal seas of Hyogo prefecture, Japan

The aim of this study was to investigate 12 perfluorinated compounds (PFCs) including perfluorinated carboxylates (C4–C12) and perfluorinated alkyl sulfonates (C4, C6, and C8) in river and seawater samples to determine contamination levels in the aquatic environment of Hyogo prefecture, Japan. High levels of perfluorohexanoic acid (PFHxA; 2300–16,000 ng/L) were detected in the Samondogawa River at Tatsumi Bridge downstream of a PFC production facility; this location also had the highest mass flow rate of PFCs (3900–29,000 kg/y). Widespread contamination of coastal waters was confirmed with PFHxA as the dominant compound. Perfluorooctanoic acid was also prevalent in coastal waters. The concentration of PFHxA in coastal seawater and the distance from the mouth of the Samondogawa River were inversely related. This discharge of high concentrations of PFHxA from the Samondogawa River may have affected concentrations of PFCs in Osaka Bay.

Association of Toxoplasma gondii oocysts with fresh, estuarine, and marine macroaggregates

The protozoan parasite Toxoplasma gondii infects a large proportion of threatened California sea otters (Enhydra lutris nereis), and is an important waterborne pathogen in humans. Contamination of coastal waters with T. gondii is thought to occur through delivery of environmentally resistant oocysts to nearshore regions via overland runoff. The objectives of this study were to evaluate whether T. gondii oocysts and surrogate microspheres attach to aggregates (≥ 0.5 mm), and whether the magnitude of aggregation depends on water type, specifically salinity. Laboratory aggregation studies were conducted by adding T. gondii oocysts and surrogate microspheres to riverine, estuarine, and marine waters, and quantifying the proportion of oocysts and surrogates in aggregate‐rich and aggregate‐free water fractions. Attachment of oocysts and surrogates to aggregates occurred in all water types, but was greater in estuarine and marine waters, with concentrations of T. gondii in aggregates enriched 3–4 orders of magnitude. Aquatic aggregates may, thus, significantly influence waterborne transport of terrestrially derived pathogens, both through enhanced settling and subsequent concentration in the benthos, as well as by facilitating ingestion by invertebrate vectors that can transmit pathogens to susceptible hosts, including sea otters and humans.

Radioisotopes Demonstrate the Contrasting Bioaccumulation Capacities of Heavy Metals in Embryonic Stages of Cephalopod Species

Cephalopods play a key role in many marine trophic food webs and also constitute alternative fishery resources in the context of the ongoing decline in finfish stocks. Most coastal cephalopod species of commercial importance migrate into shallow waters during the breeding season to lay their eggs, and are consequently subjected to coastal contamination. Eggs of common cuttlefish Sepia officinalis, European squid Loligo vulgaris, common octopus Octopus vulgaris and the sepiolid Rossia macrosoma were exposed during embryonic development to dissolved 110mAg, 109Cd, 60Co, 54Mn and 65Zn in order to determine their metal accumulation efficiencies and distribution among different egg compartments. Cuttlefish eggs, in which hard shells enclose the embryos, showed the lowest concentration factor (CF) values despite a longer duration of exposure. In contrast, octopus eggs, which are only protected by the chorionic membrane, accumulated the most metal. Uptake appears to be linked to the selective retention properties of the egg envelopes with respect to each element. The study also demonstrated that the octopus embryo accumulated 110mAg directly from the dissolved phase and also indirectly through assimilation of the contaminated yolk. These results raise questions regarding the potential contrasting vulnerability of early life stages of cephalopods to the metallic contamination of coastal waters.

Active biomonitoring in Greek coastal waters: Application of the integrated biomarker response index in relation to contaminant levels in caged mussels

An integrated approach using biomarkers and contaminant levels in mussels Mytilus galloprovincialis L. was employed to assess chemical contamination in Greek coastal waters within the framework of the MYTIMED program. Biomarkers (metallothioneins, glutathione S-transferase, catalase, acetylcholinesterase and RNA:DNA ratio) have been previously described in mussels caged at 14 sites in coastal areas influenced by different types of anthropogenic activities. This study applied a biomarker index, the Integrated Biomarker Response (IBR) to summarize biomarker responses and relate stress levels to concentrations of organochlorine compounds (PCBs, DDTs), polycyclic aromatic hydrocarbons (PAHs), and metals (Cu, Ni, Fe, Zn) measured in the mussel tissues. The IBR index indicated environmental stress at sites near cities and industries and was overall related to organic contaminants, but also elevated metal concentrations at certain sites. Slightly increased IBR values at few sites away from known pollution sources were not accompanied with increased contaminant levels suggesting stress possibly caused by natural factors. Results confirmed the usefulness of integration of biological effects measurements and chemical analysis for the assessment of chemical contamination in coastal waters.

Assessment of the AMSR-E soil moisture product over India

Soil moisture is a very important boundary parameter in numerical weather prediction at different spatial and temporal scales, controlling the exchange of water and energy between the atmosphere and land surface. Satellite-based microwave radiometric observations are considered to be the best for soil moisture remote sensing because of their high sensitivity, as well as their all-weather and day–night observation capabilities with high repeativity. In this study, an attempt has been made to assess the Advanced Microwave Scanning Radiometer--Earth Observing System (AMSR-EOS) soil moisture product over India. The AMSR-E soil moisture product has been assessed using in situ soil moisture observations made by the India Meteorological Department (IMD) during the monsoon period (May–August) for the years 2002–2006 over 18 meteorological stations. Apart from assessing AMSR-E soil moisture retrieval accuracy, this study also investigates the effect of vegetation, topography and coastal water contamination, and determines the regions where the AMSR-E soil moisture product could be useful for different applications.

Contamination and bioaccumulation of benzotriazole ultraviolet stabilizers in fish from Manila Bay, the Philippines using an ultra-fast liquid chromatography–tandem mass spectrometry

Benzotriazole ultraviolet stabilizers (BUVSs) used in plastic products, building materials and personal hygiene products were analyzed in fishes collected from Manila Bay, the Philippines. BUVSs were detected at ng g −1 level in all the fish samples, indicating their ubiquitous contamination in coastal waters. Among the targeted eight BUVSs, UV-328 was predominantly found with a mean concentration of 34.2 ng g −1 lipid weight, implying large scale production and use of this compound in the Philippines. High concentrations of ∑BUVSs were found in bumpnose trevally ( Carangoides hedlandensis), bluetail mullet (adult) ( Valamugil buchanani), common ponyfish ( Leiognathus equulus) and coral grouper (adult) ( Epinephelus corallicola) indicating their active uptake and/or lower metabolic capacity to eliminate BUVSs. Among BUVSs, UV-P showed significant positive relationship ( p < 0.05) between concentration and fish length ( r = 0.29) and fish weight ( r = 0.31). Levels of UV-P in demersal species had positive correlation with δ 15N, indicating that possibile sink of UV-P is bottom sediment in the bay, and ultimately accumulate through benthic food web rather than pelagic food web. To our knowledge, this is the first study on BUVSs distribution in fish from developing countries.

Kinetic study of the bioaccumulation of heavy metals (Cu, Pb, and Cd) in Chinese domestic oyster Ostrea plicatula

Much attention has been given to heavy metal contamination in coastal waters and on seafood safety induced by bioaccumulation of aquatic organisms in China. To obtain the kinetic characteristics of heavy metal bioaccumulation in Chinese native organisms, the accumulation and elimination of three heavy metals (Cu, Pb, and Cd) in domestic oyster Ostrea plicatula were investigated in both laboratory and field conditions. The kinetic parameters of bioaccumulation, including the uptake rate constant (K 1), elimination rate constant (K 2), bioconcentration factors (BCFs), and biological half-life (t 1/2), were estimated through nonlinear curve fitting using a two-compartment model. The results showed that the Pb and Cd metal concentration data perfectly fitted the two-compartment model, and good agreement was obtained between the predicted data and those obtained by the goodness-of-fit test. However, the Cu data did not fit the model. The modeling results indicated that the Pb and Cd concentrations in O. plicatula could reflect the contamination status of seawater and that O. plicatula is a potentially dependable indicator for monitoring heavy metal (Pb and Cd) contamination and seafood safety in the coastal areas of China.

Spatial distribution and sources of polycyclic aromatic hydrocarbons (PAHs) in green mussels (Perna viridis) from coastal areas of Peninsular Malaysia: implications for source identification of perylene

Distribution of polycyclic aromatic hydrocarbons (PAHs) was determined in green mussels (Perna viridis) from various sites in coastal waters of Peninsular Malaysia between August 2004 and January 2007, in order to assess contamination by petroleum hydrocarbons. The range of ∑PAHs detected in mussels was from 766 to 110500 (ng/g lipid wt.). High concentrations of PAHs were found in mussel tissues collected near Penang Bridge. The ratios of methyl phenanthrenes to phenanthrene (∑MP/P ratio) for Penang, Kg. Pasir Puteh and Tebing Runtuh (Johore Straits) were greater than 2, indicating extensive input of petrogenic PAHs. The results indicated that male individuals elevated more considerable concentrations of PAHs in their soft tissues in comparison to female individuals. The results of independent sample T-test showed that there were no significant differences (p > 0.05) between male and female mussels analysed in the Pasir Panjang station. Negative significant correlations (r = −0.890, p < 0.01) and (r = −0.0655, p < 0.05), were found between weight and total of PAHs in female and male species, respectively. This indicated that body weight of each individual was not affected by the PAHs concentrations. The present study proposes the use of soft tissue of Perna viridis as a biomonitor of perylene bioavailability and contamination in coastal waters of Peninsular Malaysia.

Relevance of watershed modelling to assess the contamination of coastal waters due to land-based sources and activities

To evaluate the relevance of a watershed modelling for assessing the contamination of coastal waters due to land-based sources and activities, a modelling approach, using SWAT (Soil and Water Assessment Tool) model, was implemented on the watersheds of Pen-Bé estuary and Le Croisic bay. For the past few years, various dysfunctions occurred in these shellfish farming areas located on the West coast of France. Therefore, it seemed critical to estimate the coastal watershed loadings in order to get a better understanding of the dysfunctions. In the same way, an empiric method based on water quality samplings was also carried out in order to evaluate the potential pollutant attenuation of the coastal wetlands. The results presented in this paper focus on simulated streamflows and phosphorus flows. Despite the limited existing data, which involved to integrate aggregated or averaged values for several aspects, according to the Nash and Sutcliffe efficiency criterion, the simulated flows seem close to the measurements. The simulated continuous flows appeared as a useful complement to intermittent water quality samplings and streamflow measures resulting from water monitoring networks. Moreover, by identifying the most contributory sub-watersheds, the simulations could be used to suggest priority areas of intervention for decreasing the coastal watershed loadings.

Factors controlling physico-chemical characteristics in the coastal waters off Mangalore—A multivariate approach

Water quality parameters (temperature, pH, salinity, DO, BOD, suspended solids, nutrients, PHc, phenols, trace metals—Pb, Cd and Hg, chlorophyll- a (chl- a) and phaeopigments) and the sediment quality parameters (total phosphorous, total nitrogen, organic carbon and trace metals) were analysed from samples collected at 15 stations along 3 transects off Karnataka coast (Mangalore harbour in the south to Suratkal in the north), west coast of India during 2007. The analyses showed high ammonia off Suratkal, high nitrite (NO 2-N) and nitrate (NO 3-N) in the nearshore waters off Kulai and high nitrite (NO 2-N) and ammonia (NH 3-N) in the harbour area. Similarly, high petroleum hydrocarbon (PHc) values were observed near the harbour, while phenols remained high in the nearshore waters of Kulai and Suratkal. Significantly, high concentrations of cadmium and mercury with respect to the earlier studies were observed off Kulai and harbour regions, respectively. R-mode varimax factor analyses were applied separately to surface and bottom water data sets due to existing stratification in the water column caused by riverine inflow and to sediment data. This helped to understand the interrelationships between the variables and to identify probable source components for explaining the environmental status of the area. Six factors (each for surface and bottom waters) were found responsible for variance (86.9% in surface and 82.4% in bottom) in the coastal waters between Mangalore and Suratkal. In sediments, 4 factors explained 86.8% of the observed total variance. The variances indicated addition of nutrients and suspended solids to the coastal waters due to weathering and riverine transport and are categorized as natural sources. The observed contamination of coastal waters indicated anthropogenic inputs of Cd and phenol from industrial effluent sources at Kulai and Suratkal, ammonia from wastewater discharges off Kulai and harbour, PHc and Hg from boat traffic and harbour activities of New Mangalore harbour. However, the strong seasonal currents and the seasonal winds keep the coastal waters well mixed and aerated, which help to disperse the contaminants, without significantly affecting chlorophyll- a concentrations. The interrelationship between the stations as shown by cluster analyses and depicted in dendograms, categorize the contamination levels sector-wise.

Surveillance du milieu marin et protection sanitaire des coquillages

Monitoring of marine environment and shellfish health protection. Shellfish farming is a major economic activity in the European Union, France being one of the main producing countries. Regulations were introduced to meet society’s current expectations in terms of food safety at the fishing, farming and harvesting stages, as well as during processing and marketing. Toxic algae, pathogens or pollutants may contaminate shellfish products during production and harvesting, partly because of the particular environmental conditions in which these animals are grown. Monitoring programmes were thus developed to detect the presence of biological and chemical contaminants in coastal waters and in shellfish, and to monitor the food safety of the latter.

Surfer and Beachgoer Responsiveness to Coastal Water Quality Warnings

Fecal contamination of coastal waters has led local public health agencies to issue tens of thousands of beach advisories annually and to advise against ocean swimming and surfing for 72 hours following a rain. The responsiveness of recreationists to beach advisories and the “72 hour rule” is estimated by merging four years of multi-daily surfer and beachgoer counts at one beach in Southern California with hourly data on beach advisories, recent precipitation, meteorological conditions, and the UCSB academic calendar. Both surfing and beach-going decreased significantly following rainfall in response to the 72 hour rule, showing that recreationists are willing to engage in health risk-reducing avoiding behavior. However, neither surfing nor beach-going decreased significantly during beach advisories. Surfers were significantly more responsive to beach advisories during dry periods than during wet periods, consistent with the hypothesis that recreationists are most likely to respond to a risk warning that they believe provides accurate and additional health risk information beyond what they can observe personally. Additional studies of beach advisories elsewhere could clarify whether responsiveness to beach advisories varies by recreationist demographic, recreational activity, frequency of advisories, or frequency of repeat visitation. Increasing the accuracy and additional information provided by beach advisories, as proposed by numerous authors, could lead to increased responsiveness.

The sandhopper Talitrus saltator (Crustacea: Amphipoda) as a biomonitor of trace metal bioavailabilities in European coastal waters

The amphipod crustacean Talitrus saltator is an established, easily accessible, biomonitor of trace metal bioavailabilities in coastal waters. We have carried out a geographically widespread collection of T. saltator from European shores, stretching from the north-west Atlantic through the Baltic to the Mediterranean. A primary aim of the work was to establish a database of accumulated trace metal concentrations (Cd, Cr, Cu, Fe, Mn and Zn) in this biomonitor. Statistical analysis has shown significant geographical differences in the bioavailabilities of all the metals, the most distinct being copper, iron and manganese. It has proved possible to identify unusually high accumulated concentrations of Cd, Cr, Cu, Fe, Mn and Zn in this biomonitor, indicative of high metal bioavailability at a particular site. These may serve as reference points for future biomonitoring programmes seeking to identify metal contamination in coastal waters.