Contagious Infectious Disease Research Articles

Діагностика вірусу інфекційного панкреатичного некрозу (IPNV): Огляд

Purpose. Infectious pancreatic necrosis virus (IPNV) causes highly contagious infectious pancreatic necrosis disease in rainbow trout and other salmonids. The limited number of preventive and curative remedies makes diagnosis and monitoring the only appropriate way to control the disease. Therefore, the aim of our work was to analyze the literature on methods of virus identification. Findings. The article presents data on some methods of diagnosis and study of the pathogenesis of infectious pancreatic necrosis. Emphasis is placed on modern molecular biological methods and sets of methods that are recommended for use in the procedure of disease surveillance, including the method of cell culture. Information on polymerase chain reaction methods is widely covered, both in classical modification (RT-PCR) and in real time PCR. Attention is also paid to the latest techniques, such as biosensors and loop isothermal amplification (LAMP). Originality. Data on the diagnosis and study of infectious pancreatic necrosis virus by modern molecular biological methods and disease monitoring are summarized. Promising ways to identify the virus in aquaculture farms in situ (in the field) are briefly presented. Practical value. The presented information will help fish pathologists to choose the appropriate method for diagnosing the disease, selection of constituent reactions or a combination of several techniques to improve sensitivity. Key words. IPNV, diagnostics, RT-PCR, cell culture, biosensors.

State‐of‐the‐art electrochemistry for the assessment of oxidative stress and integral antioxidant activity of biological environments

AbstractThe present review focuses on the interplay between electrochemistry and life, events on the border of electrochemistry‐biology‐life science, electrochemistry as the basis, and the information source on oxidative stress (OS) or Red/Ox state of biological systems and food to be investigated. Electroanalytical chemistry provides rapid, relatively simple, and sensitive approaches to assess the redox characteristics and antioxidant activity of biologically active compounds in various samples.OS is a relatively new physiological response concept, recognized in medicine and biology in the last three decades. This phenomenon is caused by an imbalance between (pro)oxidants and antioxidants in living organisms and it is related to the fundamental redox reactions that underlie health signaling and life processes in general. OS can contribute to many pathological conditions and diseases. In particular, it is recognized that a highly contagious infectious disease, coronavirus disease 2019, is associated with an inflammation process related to OS‐induced cellular changes. Recent years have shown a marked increase in electrochemical studies of OS and quantitation of its reductant‐oxidant markers (signaling agents), such as reactive oxygen species and antioxidants.The goal of this overview is to cover the brief scope of modern electrochemical analysis and sensor devices for monitoring biomarkers of OS and antioxidant status of biological systems. By discussing the great potential of potentiometric and voltammetric methods for human health assessment, it is hoped to bridge between recent electrochemical research and medical diagnostic treatment in the 21st century.

Time for Isoniazid Pharmacogenomic-Guided Therapy of Tuberculosis Based on NAT2 Acetylation Profiles in India.

Tuberculosis (TB), caused by a bacterial pathogen Mycobacterium tuberculosis, is a highly contagious infectious disease. India ranks top in TB burden among other countries in the world. The current treatment of TB in India includes isoniazid (INH) as the first-line drug, based on the "one dose fits all'' concept. It is widely known that INH is not equally tolerated by all patients, as the metabolization process of INH is highly dependent on the acetylation profile of an individual based on the pharmacogenomic profile of the N-acetyltransferase (NAT2) gene. Also, several experimental studies in several TB endemic countries have established that redosing of INH based on genetic profiles of TB patients of the NAT2 can help in effective TB treatment and minimize adverse drug reactions (ADRs). Moreover, acetylation phenotype-based INH dosing has been shown to be cost-effective as well as successful in terms of treatment outcomes and the increase in the quality of life of the patients. Considering a genetically heterogenous population with high vulnerability to TB, we here argue that India could lead in establishing a pharmacogenomic-guided therapy (PGT) under the INH-NAT2 model. With such an approach, not only could an innovative step towards 'End-TB' by the year 2025 be taken but a personalized medicine approach for TB treatment be initiated in India, particularly in tribal communities.

Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice.

Coronavirus disease-2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2; CoV2) is a deadly contagious infectious disease. For those who survive COVID-19, post-COVID cardiac damage greatly increases the risk of cardiomyopathy and heart failure. Currently, the number of COVID-related cases are increasing in Latin America, where a major COVID comorbidity is Chagas' heart disease, which is caused by the parasite Trypanosoma cruzi. However, the interplay between indeterminate Chagas disease and COVID-19 is unknown. We investigated the effect of CoV2 infection on heart pathology in T. cruzi infected mice (coinfected with CoV2 during the indeterminate stage of T. cruzi infection). We used transgenic human angiotensin-converting enzyme 2 (huACE2/hACE2) mice infected with CoV2, T. cruzi, or coinfected with both in this study. We found that the viral load in the hearts of coinfected mice is lower compared to the hearts of mice infected with CoV2 alone. We demonstrated that CoV2 infection significantly alters cardiac immune and energy signaling via adiponectin (C-ApN) and AMP-activated protein kinase (AMPK) signaling. Our studies also showed that increased β-adrenergic receptor (b-AR) and peroxisome proliferator-activated receptors (PPARs) play a major role in shifting the energy balance in the hearts of coinfected female mice from glycolysis to mitochondrial β-oxidation. Our findings suggest that cardiac metabolic signaling may differently regulate the pathogenesis of Chagas cardiomyopathy (CCM) in coinfected mice. We conclude that the C-ApN/AMPK and b-AR/PPAR downstream signaling may play major roles in determining the progression, severity, and phenotype of CCM and heart failure in the context of COVID.

Detection of Treponema phylotypes from digital dermatitis lesions and effect of different phylotypes on lesion size.

Bovine digital dermatitis (BDD) is a contagious infectious disease which causes lameness in dairy cows. It has a multifactorial etiology which is not yet fully understood but Treponema spp. seem to play a significant role in development of BDD lesions. This study evaluated the presence of Treponema phylotypes commonly associated with BDD (T. medium/T. vincentii, T. phagedenis and T. putidum/T. denticola), in four farms different areas in Iran. Single biopsies were taken from 113 Holstein cows with active BDD lesions (scored according to size) on the farms and polymerase chain reaction assays used to detect 16S rRNA nucleotide fragments of three BDD Treponema phylotype groups: ‘‘T. medium/T. vincentii’’, ‘‘T. phagedenis’’ and ‘‘T. putidum/T. denticola’’ (now T. pedis). Over 95.00% of samples were positive for at least one of phylotypes, with 89.00%, 91.00 %, and 66.00% of samples were positive for T. putidum/T. denticola, T. phagedenis and T. medium/T. vincentii, respectively. Out of the 113 samples, 60.00% were positive for all three phylotypes, the detection of T. putidum/T. denticola was positively associated with detection of both T. phagedenis and T. medium/T. vincentii. No association between lesion size and phylotypes identified was found but there were significant differences between farms in the proportion of each phylotypes identified. Further research is required to establish the factors influencing the proportions of individual phylotypes, especially at the farm level.

Pulmonary tularemia in a person taking immunosuppressive drugs: a literature review and a clinical case report

Ieva Radavičiūtė 1, Gintarė Žilinskaitė 1, Birutė Zablockienė 2, 3 1 Vilnius University Faculty of Medicine, Vilnius, Lithuania 2 Vilnius University Faculty of Medicine, Institute of Clinical Medicine, Clinic of Infectious diseases and Dermatovenerology, Vilnius, Lithuania 3 Vilnius University Hospital Santaros Klinikos, Center of Infectious Diseases, Vilnius, Lithuania Abstract Introduction: Aerobic Gram negative bacterium – Francisella tularensis may cause a rare zoonotic disease tularemia. The reservoir of infection are various animals and arthropods. The infection can be transmitted through the respiratory tract, skin, mucous membranes, gastrointestinal tract or by insect bites. Consequently the clinical forms of tularemia are ulcerative glands, lymphadenopathy, ocular hemorrhage, oral and pharyngeal tularemia, gastrointestinal, respiratory and typhoidal tularemia. One of the most severe forms of the disease is pulmonary tularemia, which has a high mortality rate and is particularly dangerous in patients taking immunosuppressive drugs. Clinical case: This article presents a clinical case of a patient treated for rheumatoid arthritis with a TNF-alpha inhibitor (Infliximab). He contacted a doctor in the spring of 2020 due to a dry cough. In September of 2020 a diagnosis of tularemia was confirmed after various diagnostic tests. Antibacterial treatment was effective, but the patient was recommended not to resume biologic therapy with TNF-alpha inhibitors in the setting of tularemia. Conclusion: Tularemia is a rare but easily contagious infectious disease that often takes time to diagnose. In this clinical case, tularemia was more severe in an immunosuppressed patient who developed severe lesions such as lung lesions and lymphadenopathy than in a normal tularemia case patient. For such patients it is particularly important to recognize tularemia, perform laboratory tests to diagnose the zoonosis, and decide on further treatment with immunosuppressive therapy. Keywords: Immunodeficiency; biological therapy; pneumonia, tularemia.

FAKTOR-FAKTOR YANG MEMPENGARUHI KETIDAKPATUHAN MINUM OBAT PADA PASIEN TUBERKULOSIS PARU

Background: Pulmonary tuberculosis is a contagious infectious disease caused by bacteria, which can attack the respiratory system, namely the lungs. This disease every year accounts for the highest number of deaths in the world, including developing countries. To achieve successful treatment, patients must be patient to take TB drugs to completion.Methods: This study uses a correlation approach. a total of 20 respondents with pulmonary tb, using the total sampling technique. Kai kuadrat data analysis.Result: There was a significant effect between education, knowledge, length of illness, and side effects of drugs with a value (p < 0.05).Conclusion: Education, knowledge, length of illness, and side effects of drugs have a significant effect on uncompliance to taking medication in pulmonary tb patients in the kapongan health center work area

HUBUNGAN PERILAKU DAN LINGKUNGAN RUMAH DENGAN TB PARU DI WILAYAH KERJA PUSKESMAS PANYABUNGAN JAE

ABSTRACT
 Pulmonary tuberculosis or pulmonary tuberculosis is a contagious infectious disease that attacks the lungs and bronchi. This disease is caused by mycobacterium tuberculosis which spreads through the air of human respiratory discharges. In 2017, the figure of Case Notification Rate / CNR (new case) of pulmonary TB (+) in North Sumatra was 104.3 / 100,000 population and Mandailing Natal District became an area second place with CNR of 187 / 100,000 population. One of the factors that can affect the recovery of pulmonary TB include behaviors such as knowledge, attitudes and actions as well as the environment such as ventilation area and occupancy density. The purpose of this study was to determine the relationship of behavior and home environment to the incidence of pulmonary TB in Panyabungan Jae Public Health Center in 2019. This type of research uses analytic survey method with cross sectional approach. The sample in this study were 56 people taken by total sampling technique. The results showed that the majority of respondents have less knowledge, negative attitudes, actions are not good, the area of ​​ventilation of the house does not meet the requirements and the density of dense occupancy with the majority of the occurrence of pulmonary TB. Bivariate test results found that there is a relationship between knowledge (p = 0.003), attitude (p = 0.010), actions (p = 0.013), ventilation area (p = 0.003) and density (p = 0.017) with the incidence of pulmonary TB in the Panyabungan Jae Public Health Center. It is expected that the community in the Panyabungan Jae Puskesmas area will be more active in carrying out the examination and treatment of new TB and following the management of pulmonary TB provided by health care workers. The next researcher is expected to be able to develop research on the factors that cause pulmonary TB in a region

BAG2-activated cell autophagy and mir-27b dynamic regulation mechanism during Mycobacterium tuberculosis infection.

Tuberculosis is a highly contagious infectious disease. Mycobacterium tuberculosis infection is the main cause of tuberculosis. During the infection of M. tuberculosis, the expression of the resistance gene BAG2 will change, and miR-27b will play a certain role in dynamic regulation. The purpose of this article is to explore in-depth the effect of BAG2 on cell autophagy during M. tuberculosis infection and the dynamic regulatory mechanism of miR-27b on BAG2 activated cell autophagy. Fifty rats were used as experimental subjects, and M. tuberculosis strains H37Ra and H37Rv were implanted into the rats. Fluorescence quantitative PCR was used to detect the dynamic changes of BAG2 and miR-27b expression levels in rats and the regulatory effect of miR-27b on BAG2, and the effect of changes in BAG2 expression levels on cell autophagy was studied by immunoblotting. The results showed that after M. tuberculosis-infected macrophages, the expression level of BAG2 decreased from (284.24±6.31) to (156.48.24±4.49), and the expression level of miR-27b was increased from (43.72±3.35) to (78.35± 4.17), the apoptosis rate increased by 17.8%, and the autophagy rate increased by 20.6%. Therefore, it can be seen that the up-regulation of miR-27b expression level during M. tuberculosis infection will inhibit BAG2 expression, thereby promoting cell autophagy and apoptosis to reduce the survival rate of M. tuberculosis.

CME: Fever in Travelers Returning from the Tropics

CME: Fever in Travelers Returning from the Tropics Abstract. With rising numbers of travelers to tropic countries consultations for suspected tropical infections are on the rise in primary care and emergency units. The aim is to identify potentially life-threatening and highly contagious infectious diseases. In addition, counseling on the prevention of tropical diseases is taking on an important role. This article intends to provide an overview of the most important infections acquired in the tropics and support for clinical practice.

Experience with COVID-19 convalescent plasma provides vital guidance to future pandemics.

Experience with COVID-19 convalescent plasma provides vital guidance to future pandemics.

Population density impact on COVID-19 mortality rate: A multifractal analysis using French data

The current COVID-19 pandemic caught everyone off guard and is an excellent case study to investigate the real impact of population density on emerging highly contagious infectious diseases. The relationship between the threat of COVID-19 and population density has been widely debated not only in scientific articles, but also in magazines and reports around the world. It appeared both in the columns of experts and in the speeches of politicians, yet without reaching any consensus. In this study, using COVID-19 data from France, we try to shed light on this debate. An alternative density measure, weighted by population, is used. This novel density measure clearly outperforms the commonly used density in terms of relationship with COVID-19 deaths and proved to be competitive with some of the best known predictors, including population. A multifractal analysis, characterizing different space distributions of population in France, is used to further understand the relation between density and COVID-19 mortality rate.

Use of Lactobacillus plantarum in Preventing Clinical Cases of American and European Foulbrood in Central Italy

American and European Foulbrood (AFB and EFB) are considered the most contagious infectious diseases affecting honeybees worldwide. New sustainable strategies need to be implemented for their prevention and control, and probiotics may represent one solution to investigate. In our study, we evaluated the efficacy of one strain of Lactobacillus plantarum (L. plantarum) isolated from northern Italy, orally administered to the bees for AFB and EFB prevention. From March to September 2014, a total of 979 honeybee colonies (9.6% of Viterbo province—Central Italy) were taken under observation from 22 apiaries. Overall prevalence of AFB was 5.3% in treated colonies and 5.1% in the untreated ones. On the contrary, EFB prevalence was lower in the treated colonies (2.5%) compared to the untreated ones (4.5%). L. plantarum showed a significant effect in reducing insurgence of cases of EFB up to 35 days after the end of the treatment (p-value: 0.034). Thanks to this study we could investigate the preventive efficacy of L. plantarum in controlling AFB and EFB, and obtain official data on their clinical prevalence in Central Italy.

Brucella induces heme oxygenase-1 expression to promote its infection.

Brucellosis is a zoonotic and contagious infectious disease caused by Brucella spp, which causes substantial economic losses to animal husbandry and leads to severe public health problems. Brucella have evolved multiple strategies to escape host immunity and survive within host cells. Elucidating the immune evasion strategies during Brucella infection will facilitate the control of brucellosis. The host enzyme, heme oxygenase-1 (HO-1), is a multifunctional protein that functions during inflammatory diseases and microbial infections. However, how HO-1 functions during Brucella infection is rarely studied. In this study, we evaluated the role of HO-1 during Brucella infection. We found that Brucella infection induced HO-1 expression in macrophages. We further showed that HO-1 was regulated by PI3K, AMPK kinase, and nuclear erythroid-related factor 2 (Nrf2) in macrophages. Interestingly, knocking out HO-1 or inhibiting the activity of HO-1 significantly decreased Brucella intracellular growth. Inducing the expression of HO-1 by treatment with CoPP promoted Brucella intracellular growth. Mechanistic analyses indicated that the effect of HO-1 was not meditated by HO-1 metabolites, but by decreasing the production of reactive oxygen species (ROS), TNF-α, and IL-1β. Moreover, Brucella induced HO-1 expression in bone marrow-derived macrophages (BMDMs) and mice. When the expression of HO-1 was knocked down in BMDMs, the intracellular survival of Brucella was reduced. Furthermore, the induction of HO-1 by CoPP significantly increased bacterial loads in vivo. Thus, we demonstrated that Brucella induced HO-1 expression to promote its survival and growth in vitro and in vivo. This study also identified HO-1 as a novel innate immune evasion factor during Brucella infection.

Presence of SARS-CoV-2 on food surfaces and public space surfaces in three districts of Lima, Peru.

The aim of this study was to determine the presence of SARS-CoV-2 on food surfaces and surfaces in public spaces in 3 districts of Lima, Peru. A cross-sectional descriptive study was carried out in three districts of the Lima metropolitan area. Surfaces that were most exposed to users were selected. Samples were swabbed for 4 weeks and transported to the laboratory to determine the presence of the virus. One thousand ninety-five inert surface samples and 960 food surface samples were evaluated for the identification of SARS-CoV-2 by the real time-PCR molecular test, whereby only one sample from an automated teller machine was positive. Most of the inert and food surfaces evaluated did not show the presence of SARS-CoV-2 during the time of sample collection. Despite the negative results, the frequency of disinfection and hygiene measures on high-contact surfaces should be maintained and increased to prevent other highly contagious infectious diseases.

Cell lysis analysis for respiratory viruses through simulation modeling

An ordinary system of differential equations leading to a simulation model is propose as methodological approach to analysis the incidence of infectious-contagious diseases, in this case using SARS-CoV-2 virus as pathogenic model. The dynamics of the model are drive by the interaction between susceptible cells contemplating respiratory epithelial cells and viral infection mediated by two types of lysis response. To perform the simulations, values of some variables and parameters were selected from referenced sources, considering that previous reports suggested that the viral load in the lower respiratory tract might reach its peak in the second week after the beginning of disease symptoms. The scenarios described in the simulations evidence the performance of the cell lysis response from susceptible cells that have been infected. The recommend model shows that an excess response from both the original virus and the mutated virus leads to an increase in the approximate time to control viral infection within the organism.

Sarampo: protocolo de atuação num Hospital Português

Introduction Measles is a highly contagious infectious disease caused by a paramyxovirus. In the last six decades, the promotion of vaccination against this disease has drastically reduced its incidence. Despite this, in 2016, around seven million new infections were diagnosed. Since healthcare workers have an increased risk of infection, the promotion of vaccination by the Occupational Health Services represents one of the main strategies to consolidate the eradication of this pathology in Portugal. Objectives Review the clinical aspects and prevention measures of Measles with a focus on vaccination and present post-exposure prophylaxis measures for healthcare workers in a Portuguese hospital. Methodology It was performed a literature search about the clinical and preventive aspects of Measles using the main guidelines of organizations, such as the Direção-Geral da Saúde and Centers for Disease Control and Prevention, and through identification of scientific articles on PubMed/MEDLINE databases. Results/Discussion This infection has an incubation period that can last between seven to twenty-one days, and it’s followed by the prodromal, exanthematic, and recovery phases. Infected patients are contagious four days before and four days after the rash appears. The diagnosis is based on clinical, laboratory and epidemiological criteria. A confirmed case of Measles must meet clinical and laboratory criteria. The treatment is based on supportive care, to prevent dehydration. Vaccination is the best preventive measure against this disease. All healthcare workers without a credible history of Measles should have two vaccine doses. In an outbreak context, exposed workers must be identified and the Occupational Health Departments must apply appropriate post-exposure prophylaxis measures, as well as define which employees require work avoidance and for how long. Conclusion The existence of well-defined action plans for healthcare workers who had contact with infected patients is essential to minimize the occurrence of intra-hospital transmission chains and the subsequent infection of people at higher risk of complications from the disease. The presented protocol allows acting clearly and concisely when dealing with a suspected or confirmed Measles case and its contacts and provides a succinct review of the clinical, therapeutic and preventive data of this pathology. Keywords: Measles; Measles vaccine; Vaccination; Outbreak; Prevention; Occupational Health.

Андрологические аспекты новой коронавирусной инфекции COVID-19

COVID-19 is a new highly contagious infectious disease caused by the SARS-CoV-2. The World Health Organization (WHO) on March 11, 2020, has declared the novel coronavirus (COVID-19) outbreak a global pandemic. More attention is currently paid to the fact that men are more at risk for worse outcomes. In addition, SARS-CoV-2 can infect the testes, potentially affecting testosterone production, as well as having a negative influence on the reproductive potential. Our aim was to review the current concepts of the possible influence of testosterone levels on the pathogenesis of COVID-19 in men and to present the available data on the impact of COVID-19 on the structure and function of the testis. Based on the analysis of 72 articles using the MEDLINE database (PubMed), it can be concluded that testosterone is involved in the co-regulation of the synthesis of angiotensin-converting enzyme-2 and transmembrane serine protease-2, facilitating the penetration of SARS-CoV-2 into target cells and promoting easier infection in men. On the other hand, low testosterone levels increase the risk of cardiopulmonary complications. Hypogonadism appears to be an important unfavorable prognostic factor for the disease. Orchitis is a reported complication of COVID-19. Damage to testicular tissue is possible due to direct invasion by a virus, a secondary autoimmune reaction, hyperthermia and thrombosis of testicular microvessels. Prophylaxis of possible vertical and sexual transmission of infection is recommended. Despite the available data, further studies are required to assess the definite role of androgens in the course of infection and the influence of SARS-CoV-2 on male reproductive potential.

Энтеровирусный везикулярный стоматит как проявление энтеровирусной инфекции. Лекция

Lesions of the oral mucosa may be a sign of systemic human diseases. Most of manifested in the oral cavity diseases are benign, but it is necessary to differentiate them from more serious diseases. Dentists should know the manifestations and recent advances in the diagnosis and treatment of oral diseases, among which are viral infections, especially highly contagious, which include hand-foot-and-mouth disease. In order to prevent its spread and to identify in time possible complications, dentist should have deep knowledge about it. Hand-foot-and-mouth disease is a highly contagious viral infectious disease caused by various enteroviruses. Viruses of the genus Enterovirus are RNA genomic. It is necessary to know the main stages of the reproduction of RNA genomic viruses in the human body. 1. Adsorption on a cell (adhesion) – an interaction between specific receptors of virions and surface molecules of sensitive cells. 2. Penetration into the cell and "undressing" can be carried out in two ways: endocytosis and translocation. 3. The synthesis of viral particles of RNA-viruses (translation) – as a result specific proteins for viral replication are synthesized. 4. Complete assembly of RNA-viruses. 5. Release of daughter virions from the cell. The source of infection can be both a sick person and a virus carrier. Asymptomatic carriage can last for weeks, sometimes months. The most intense pathogen is released in the first days of the disease. The predominant morbidity of children speaks about the high natural susceptibility of a human. For diseases, which are caused by enteroviruses, polymorphism of clinical manifestations is specific. In most cases, the violation of the general condition is insignificant and the course of the disease is benign. The initial phase is usually followed by enanthema and erythematous, papular or vesicular lesions of the skin, localized mainly on the palms and soles, as well as vesicles in the oral cavity. Enanthema usually precedes the appearance of a skin rash. Oral lesions may occur without skin symptoms. Diagnosis of enteroviral vesicular stomatitis is based on anamnestic and clinical data. There are many diagnostic tests available, but PCR of throat and vesicular fluid swabs, if available, is one of the most effective. After an illness, in most cases, immunity is formed, the possibility of developing cross-immunity to other types of the virus is not excluded

Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies.

African swine fever (ASF) is an acute and highly contagious infectious disease caused by the African swine fever virus (ASFV). Currently, there is no vaccine against ASF worldwide, and no effective treatment measures are available. For this reason, developing a simple, rapid, specific, and sensitive serological detection method for ASFV antibodies is crucial for the prevention and control of ASF. In this study, a 1:1 mixture of gold-labeled p30 and p72 probes was used as the gold-labeled antigen. The p30 and p72 proteins and their monoclonal antibodies were coated on a nitrocellulose membrane (NC) as a test (T) line and control (C) line, respectively. A colloidal-gold dual immunochromatography strip (ICS) for ASFV p30 and p72 protein antibodies was established. The results showed that the colloidal-gold dual ICS could specifically detect ASFV antibodies within 5-10min. There was no cross-reaction after testing healthy pig serum; porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease type A virus (FMDV-A), foot-and-mouth disease type O virus (FMDV-O), porcine circovirus type 2 (PCV-2), and classical swine fever virus (CSFV) positive sera. A positive result was obtained only for the positive control P1. The sensitivity of the test strips was 1:256, which was equivalent to that of commercially ELISA kits. Their coincidence rate with the two commercial ASFV ELISA antibodies detection kits was higher than 98%. The test strips were stably stored at 18-25°C and 4°C for 4 and 6months, respectively. The colloidal-gold dual ICS prepared in this study had high sensitivity and specificity and were characterized by rapid detection, simple operation, and easy interpretation of results. Therefore, they are of great significance to diagnose, prevent, and control African swine fever. KEY POINTS: • We establish an antibody detection that is quick and can monitor an ASF infection. • We observe changes in two protein antibodies to dynamically monitor ASF infection. • We use diversified detection on a single test strip to detect both antibodies.